FastQCFastQC Report
Sat 14 Jan 2017
SRR2934824.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934824.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116955
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32192.7523406438373734No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCC9010.7703817707665341No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8920.7626865033559916No Hit
CCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC7360.6293018682399214No Hit
GCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC6270.5361036296011287No Hit
CTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGCT5340.456585866358856No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5280.451455688085161No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.2479586165619255No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCG2780.2376982600145355No Hit
TCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC2660.22743790346714549No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.21204736864606044No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGC2360.20178701209867042No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.1915266555512804No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.1881065367021504No Hit
ACTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC2180.1863964772775854No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.18126629900389038No Hit
TGGTTTTTTTTTTGATTTTTCTTTTCTTCTTTAAAAGATTTATTTATGTAT1940.16587576418280534No Hit
ACCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTG1540.13167457569150529No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTC1330.11371895173357274No Hit
CGCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTG1270.10858877345987772No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGT1190.10174853576161771No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGGA302.1487576E-645.0000044
GTTTAGG302.1487576E-645.0000042
TACGGGG302.1487576E-645.0000044
AGAACAG302.1487576E-645.0000041
CAGGGCC302.1487576E-645.0000045
GGTATTA302.1487576E-645.0000048
GATAATA253.8692793E-545.09
CGAAAGG253.8692793E-545.02
AACTTCC207.007296E-445.023
GAACTAT253.8692793E-545.09
GCCGGTA207.007296E-445.022
ACGGGTA207.007296E-445.05
TCCTAGA207.007296E-445.044
ACGGGGC207.007296E-445.05
CGTTATT800.045.01
CGGGGCA253.8692793E-545.06
TCGTGGG207.007296E-445.03
CGAGGGT253.8692793E-545.04
GACGGGT207.007296E-445.04
TAGGGCA351.1994598E-745.05
Produced by FastQC (version 0.11.5)