##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934824.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	116955
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81897310931555	33.0	31.0	34.0	30.0	34.0
2	31.932546705998032	33.0	31.0	34.0	30.0	34.0
3	31.927134367919287	33.0	31.0	34.0	30.0	34.0
4	35.69172758753367	37.0	35.0	37.0	33.0	37.0
5	33.202624941216705	37.0	35.0	37.0	30.0	37.0
6	34.218511393270916	37.0	35.0	37.0	28.0	37.0
7	35.47959471591638	37.0	35.0	37.0	32.0	37.0
8	35.44917275875336	37.0	35.0	37.0	33.0	37.0
9	37.539489547261766	39.0	37.0	39.0	35.0	39.0
10	37.006823137104014	39.0	37.0	39.0	33.0	39.0
11	36.84306784660767	39.0	37.0	39.0	33.0	39.0
12	37.06619640032491	39.0	37.0	39.0	34.0	39.0
13	36.95580351417212	39.0	37.0	39.0	33.0	39.0
14	37.97598221538198	40.0	37.0	41.0	33.0	41.0
15	38.161566414432905	40.0	37.0	41.0	33.0	41.0
16	38.142422299174896	40.0	37.0	41.0	33.0	41.0
17	38.058304476080544	40.0	37.0	41.0	33.0	41.0
18	37.894335428156126	39.0	37.0	40.0	34.0	41.0
19	37.59973494078919	39.0	36.0	40.0	34.0	41.0
20	37.36782523192681	39.0	35.0	40.0	33.0	41.0
21	37.24738574665469	39.0	35.0	40.0	33.0	41.0
22	37.16861185926211	39.0	35.0	40.0	33.0	41.0
23	36.9580436920183	39.0	35.0	40.0	33.0	41.0
24	36.66618785002779	38.0	35.0	40.0	32.0	41.0
25	36.456431961010644	38.0	35.0	40.0	31.0	41.0
26	36.686058740541235	38.0	35.0	40.0	32.0	41.0
27	36.59384378607157	38.0	35.0	40.0	32.0	41.0
28	36.630644264888204	38.0	35.0	40.0	32.0	41.0
29	36.61191911418922	38.0	35.0	40.0	32.0	41.0
30	36.265426873583856	38.0	35.0	40.0	31.0	41.0
31	36.064794151596764	38.0	35.0	40.0	31.0	41.0
32	35.758659313411144	38.0	35.0	40.0	30.0	41.0
33	35.45756915052798	38.0	35.0	40.0	27.0	41.0
34	35.302475311017055	38.0	35.0	40.0	25.0	41.0
35	35.0828267282288	38.0	35.0	40.0	23.0	41.0
36	34.856765422598436	38.0	35.0	40.0	22.0	41.0
37	34.83226882134154	38.0	35.0	40.0	22.0	41.0
38	34.70298832884443	38.0	35.0	40.0	21.0	41.0
39	34.519900816553374	38.0	34.0	40.0	20.0	41.0
40	34.49332649309564	38.0	35.0	40.0	20.0	41.0
41	34.37062117908597	38.0	34.0	40.0	18.0	41.0
42	34.35194733016972	38.0	34.0	40.0	18.0	41.0
43	34.240819118464366	38.0	34.0	40.0	18.0	41.0
44	34.12788679406609	38.0	34.0	40.0	18.0	41.0
45	34.13683040485657	38.0	34.0	40.0	18.0	41.0
46	34.01639946988158	38.0	34.0	40.0	18.0	41.0
47	33.94762087982557	38.0	34.0	40.0	17.0	41.0
48	33.92003762130734	38.0	34.0	40.0	17.0	41.0
49	33.89631909708862	37.0	34.0	40.0	16.0	41.0
50	33.79899106493951	37.0	34.0	40.0	15.0	41.0
51	32.92022572784404	36.0	32.0	40.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	5.0
11	1.0
12	4.0
13	4.0
14	6.0
15	11.0
16	24.0
17	32.0
18	77.0
19	108.0
20	219.0
21	359.0
22	567.0
23	900.0
24	1315.0
25	1854.0
26	2316.0
27	2672.0
28	2587.0
29	2401.0
30	2299.0
31	2717.0
32	3254.0
33	4473.0
34	7498.0
35	11112.0
36	11996.0
37	14512.0
38	22023.0
39	21601.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.849771279551966	22.86434953614638	23.025950151767773	20.25992903253388
2	39.27578983369672	22.66341755375999	23.334615877901758	14.72617673464153
3	25.971527510580994	22.615535889872174	38.14544055405925	13.267496045487581
4	23.511607028344237	23.600530118421613	37.076653413706126	15.811209439528023
5	17.623872429566926	32.19699884570989	34.95788978667009	15.221238938053098
6	20.50617758967124	31.02475311017058	36.40631011927665	12.062759180881535
7	62.677953058868795	8.135607712368005	24.140053866871874	5.046385361891326
8	61.20046171604463	7.254072079004746	24.566713693300844	6.97875251164978
9	56.402034970715235	9.454063528707621	27.562737805138728	6.581163695438416
10	30.013252960540378	23.880124834337995	33.056303706553805	13.050318498567826
11	21.10726347740584	24.021204736864608	36.47043734769783	18.40109443803172
12	20.391603608225388	21.33641144029755	41.51425762045231	16.757727331024753
13	17.593091359924756	25.433713821555298	42.408618699499804	14.564576119020137
14	14.133641144029754	28.078320721645078	39.99828994057544	17.789748193749734
15	12.335513659099654	25.45081441580095	45.48929075285366	16.724381172245735
16	13.296567055705186	25.92193578726861	42.17605061775897	18.60544653926724
17	13.799324526527295	26.218631097430634	37.58967124107563	22.39237313496644
18	14.728741823778376	25.862938737121116	42.19828139027831	17.210038048822195
19	15.760762686503357	27.074515839425423	39.35872771578812	17.8059937582831
20	16.904792441537346	28.296353298277115	38.77901757086059	16.019836689324954
21	15.14001111538626	28.04155444401693	40.14193493223889	16.676499508357914
22	13.562481296225043	28.121927236971484	36.47043734769783	21.84515411910564
23	14.206318669573767	26.956521739130434	37.239109059039805	21.598050532255996
24	16.791928519516052	25.672267111282114	38.04882219657133	19.486982172630498
25	13.587277157881235	28.08772604848018	36.4721474071224	21.852849386516183
26	13.5547860288145	29.18301910991407	38.51994356803899	18.74225129323244
27	17.370783634731307	27.612329528451113	37.687999657988115	17.328887178829465
28	14.66461459535719	26.953101620281306	37.80257363943397	20.579710144927535
29	14.882647171989227	25.402932751913127	38.611431747253214	21.102988328844425
30	19.474156726946262	28.25958702064897	34.447437048437436	17.818819203967337
31	18.228378436150656	29.133427386601685	33.24526527296823	19.392928904279426
32	16.809884143473987	28.381001239793086	32.50309948270703	22.30601513402591
33	19.28947030909324	30.158608011628402	30.309948270702407	20.241973408575948
34	16.125860373647985	26.352015732546707	34.36535419605831	23.156769697746995
35	15.67696977469967	26.70941815228079	33.51203454320037	24.101577529819163
36	21.24150314223419	29.67551622418879	31.2778419050062	17.805138728570817
37	19.94185797956479	27.663631311188063	33.84378607156599	18.55072463768116
38	17.571715617117693	29.717412680090632	28.37330597238254	24.337565730409132
39	17.137360523278183	30.11585652601428	32.50737463126844	20.2394083194391
40	18.94061818648198	25.981787867128382	35.36915907827797	19.708434868111667
41	16.658543884399982	27.482365012184175	31.338549014578255	24.520542088837587
42	19.389508785430294	27.220725920225732	31.96870591253046	21.421059381813517
43	21.468941045701335	26.08439143260228	32.21580949938011	20.230858022316276
44	19.115044247787612	26.375101534778334	30.79304005814202	23.716814159292035
45	19.414304647086485	25.036125005343933	31.693386345175494	23.85618400239408
46	20.260784062246163	25.807361805822755	33.67791030738318	20.253943824547903
47	16.63716814159292	26.372536445641487	35.911247915865076	21.079047496900515
48	17.079218502842973	24.058826044205038	34.96045487580693	23.901500577145054
49	19.293745457654655	23.235432431276987	35.95143431234236	21.519387798726004
50	17.488777735026293	23.154204608610147	35.004916420845625	24.352101235517935
51	16.321662177760675	22.972083279893976	31.858407079646017	28.847847462699328
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1284.0
1	1800.0
2	2316.0
3	3835.0
4	5354.0
5	3524.0
6	1694.0
7	1803.5
8	1913.0
9	2001.0
10	2089.0
11	2054.0
12	2019.0
13	1962.5
14	1906.0
15	1846.0
16	1786.0
17	1499.5
18	1213.0
19	1208.0
20	1203.0
21	1064.0
22	925.0
23	1024.5
24	1124.0
25	1172.0
26	1224.0
27	1228.0
28	1287.0
29	1346.0
30	1440.5
31	1535.0
32	1670.5
33	1806.0
34	2065.0
35	2324.0
36	2378.5
37	2433.0
38	2653.5
39	2874.0
40	3386.5
41	3899.0
42	5088.5
43	6278.0
44	6687.5
45	7097.0
46	8530.5
47	9964.0
48	11595.5
49	13227.0
50	12849.0
51	12471.0
52	9918.5
53	7366.0
54	5970.0
55	4574.0
56	3780.0
57	2986.0
58	2738.0
59	2490.0
60	2321.5
61	2153.0
62	1927.5
63	1702.0
64	1430.0
65	1158.0
66	964.0
67	770.0
68	645.0
69	520.0
70	420.5
71	321.0
72	252.5
73	184.0
74	154.0
75	83.0
76	42.0
77	33.0
78	24.0
79	15.0
80	6.0
81	5.5
82	5.0
83	3.0
84	1.0
85	0.5
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	116955.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.959941857979565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.38469101635046	35.87020648967552
2	9.193037398492407	8.634090034628704
3	3.856378136265977	5.4328587918430165
4	2.2158697789592514	4.162284639391219
5	1.6659990532027238	3.9117609336924457
6	1.2454025709187575	3.5090419392073877
7	1.061505407669058	3.48937625582489
8	0.901278176322785	3.385917660638707
9	0.7355886529987983	3.1088880338591767
>10	2.6546739011689304	18.11722457355393
>50	0.04551909981428207	1.4321747680731907
>100	0.027311459888569242	2.5873199093668506
>500	0.010924583955427697	3.6065153264075924
>1k	0.001820763992571283	2.7523406438373734
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3219	2.7523406438373734	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCC	901	0.7703817707665341	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	892	0.7626865033559916	No Hit
CCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	736	0.6293018682399214	No Hit
GCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	627	0.5361036296011287	No Hit
CTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGCT	534	0.456585866358856	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	528	0.451455688085161	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	290	0.2479586165619255	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCG	278	0.2376982600145355	No Hit
TCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	266	0.22743790346714549	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	248	0.21204736864606044	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGC	236	0.20178701209867042	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	224	0.1915266555512804	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	220	0.1881065367021504	No Hit
ACTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	218	0.1863964772775854	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	212	0.18126629900389038	No Hit
TGGTTTTTTTTTTGATTTTTCTTTTCTTCTTTAAAAGATTTATTTATGTAT	194	0.16587576418280534	No Hit
ACCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTG	154	0.13167457569150529	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTC	133	0.11371895173357274	No Hit
CGCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTG	127	0.10858877345987772	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGT	119	0.10174853576161771	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.48480184686417854	0.0
2	0.0	0.0	0.0	2.1700654097729895	0.0
3	0.0	0.0	0.0	2.9507075370869136	0.0
4	0.0	0.0	0.0	3.9245863793766835	0.0
5	0.0	0.0	0.0	6.218631097430635	0.0
6	0.0	0.0	0.0	7.5199863195246035	0.0
7	0.0	0.0	0.0	8.889743918601171	0.0
8	0.0	0.0	0.0	11.085460219742636	0.0
9	0.0	0.0	0.0	11.847291693386346	0.0
10	0.0	0.0	0.0	13.739472446667522	0.0
11	0.0	0.0	0.0	17.404129793510325	0.0
12	0.0	0.0	0.0	19.516053182848104	0.0
13	0.0	0.0	0.0	20.305245607284853	0.0
14	0.0	0.0	0.0	20.588260442050363	0.0
15	0.0	0.0	0.0	21.16540549784105	0.0
16	0.0	0.0	0.0	22.502671967850883	0.0
17	0.0	0.0	0.0	24.099012440682312	0.0
18	0.0	0.0	0.0	25.83814287546492	0.0
19	0.0	0.0	0.0	26.907785045530332	0.0
20	0.0	0.0	0.0	27.808131332563807	0.0
21	0.0	0.0	0.0	29.023983583429523	0.0
22	0.0	0.0	0.0	30.135522209396775	0.0
23	0.0	0.0	0.0	31.152152537300672	0.0
24	0.0	0.0	0.0	31.813945534607328	0.0
25	0.0	0.0	0.0	32.35688940190671	0.0
26	0.0	0.0	0.0	32.82801077337437	0.0
27	0.0	0.0	0.0	33.259800778077036	0.0
28	0.0	0.0	0.0	33.675345218246335	0.0
29	0.0	0.0	0.0	34.07549912359455	0.0
30	0.0	0.0	0.0	34.50130392031123	0.0
31	0.0	0.0	0.0	34.86298148860673	0.0
32	0.0	0.0	0.0	35.20328331409517	0.0
33	0.0	0.0	0.0	35.50510880253089	0.0
34	0.0	0.0	0.0	35.818049677226284	0.0
35	0.0	0.0	0.0	36.12158522508657	0.0
36	0.0	0.0	0.0	36.340472831430894	0.0
37	0.0	0.0	0.0	36.617502458210424	0.0
38	0.0	0.0	0.0	36.898807233551366	0.0
39	0.0	0.0	0.0	37.18866230601513	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAGGGA	30	2.1487576E-6	45.000004	4
GTTTAGG	30	2.1487576E-6	45.000004	2
TACGGGG	30	2.1487576E-6	45.000004	4
AGAACAG	30	2.1487576E-6	45.000004	1
CAGGGCC	30	2.1487576E-6	45.000004	5
GGTATTA	30	2.1487576E-6	45.000004	8
GATAATA	25	3.8692793E-5	45.0	9
CGAAAGG	25	3.8692793E-5	45.0	2
AACTTCC	20	7.007296E-4	45.0	23
GAACTAT	25	3.8692793E-5	45.0	9
GCCGGTA	20	7.007296E-4	45.0	22
ACGGGTA	20	7.007296E-4	45.0	5
TCCTAGA	20	7.007296E-4	45.0	44
ACGGGGC	20	7.007296E-4	45.0	5
CGTTATT	80	0.0	45.0	1
CGGGGCA	25	3.8692793E-5	45.0	6
TCGTGGG	20	7.007296E-4	45.0	3
CGAGGGT	25	3.8692793E-5	45.0	4
GACGGGT	20	7.007296E-4	45.0	4
TAGGGCA	35	1.1994598E-7	45.0	5
>>END_MODULE