FastQCFastQC Report
Sat 14 Jan 2017
SRR2934823.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934823.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276807
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51471.859418295057567No Hit
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC18180.6567752983125427No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCC15150.5473127485937855No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8310.3002091710108487No Hit
GCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC8150.2944289703656338No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8010.28937129480107077No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6780.24493600234098126No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.2236215124617513No Hit
TCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC5880.2124223737116475No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.1979718720986102No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA5240.18930157113078788No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG4800.1734060193564469No Hit
ACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG4270.15425910471917256No Hit
CTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGCT4080.14739511645297987No Hit
ACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC3810.13764102786417973No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3600.13005451451733518No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGC3440.12427431387212029No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3320.1199391633882091No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.11777158814625353No Hit
GGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG3130.11307517512201641No Hit
GCCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG2940.10621118685582374No Hit
AGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG2920.10548866177517188No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTC2900.10476613669452No Hit
CGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG2900.10476613669452No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGG253.8831087E-545.024
ATGGGTC253.8831087E-545.05
ATGGGTA351.2074634E-745.05
CGGGTGA207.024E-445.06
AACGTAG207.024E-445.01
AGGTACG253.8831087E-545.01
AACCGAG207.024E-445.01
GCCTTCG253.8831087E-545.023
ACCGGGA207.024E-445.04
TTGTGCG253.8831087E-545.01
GCGAAGG406.7830115E-945.02
TATGGGT351.2074634E-745.04
TCGGGTA253.8831087E-545.027
CCGGAAT207.024E-445.042
AATGGCC207.024E-445.019
CCTAAGG207.024E-445.02
ACGGGTA253.8831087E-545.05
GCCATCG253.8831087E-545.022
AACGGGC253.8831087E-545.04
ACGATAG253.8831087E-545.01
Produced by FastQC (version 0.11.5)