##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934823.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	276807
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56120690589472	31.0	31.0	34.0	30.0	34.0
2	31.67829932046516	31.0	31.0	34.0	30.0	34.0
3	31.66886314291185	31.0	31.0	34.0	30.0	34.0
4	35.50139266709296	37.0	35.0	37.0	33.0	37.0
5	33.210612448384616	37.0	35.0	37.0	30.0	37.0
6	34.08044232985438	37.0	35.0	37.0	28.0	37.0
7	35.31911765237151	37.0	35.0	37.0	32.0	37.0
8	35.240409382710695	37.0	35.0	37.0	33.0	37.0
9	37.23955319049013	39.0	37.0	39.0	34.0	39.0
10	36.68880483513784	39.0	35.0	39.0	32.0	39.0
11	36.53411944062108	39.0	35.0	39.0	32.0	39.0
12	36.854656854776074	39.0	37.0	39.0	33.0	39.0
13	36.80980972302001	39.0	37.0	39.0	33.0	39.0
14	37.69128670878988	39.0	37.0	41.0	33.0	41.0
15	37.868941175620556	39.0	37.0	41.0	33.0	41.0
16	37.85227252200992	40.0	37.0	41.0	33.0	41.0
17	37.81213986640511	39.0	37.0	41.0	33.0	41.0
18	37.69510525384112	39.0	37.0	40.0	33.0	41.0
19	37.498419476386076	39.0	36.0	40.0	33.0	41.0
20	37.322564097006214	39.0	35.0	40.0	33.0	41.0
21	37.25669871065399	39.0	35.0	40.0	33.0	41.0
22	37.20980683291969	39.0	35.0	40.0	33.0	41.0
23	36.96841842872471	39.0	35.0	40.0	32.0	41.0
24	36.63861463041036	38.0	35.0	40.0	31.0	41.0
25	36.41936800731195	38.0	35.0	40.0	31.0	41.0
26	36.71194731347112	38.0	35.0	40.0	32.0	41.0
27	36.66745060637918	38.0	35.0	40.0	32.0	41.0
28	36.728012658639415	39.0	35.0	40.0	32.0	41.0
29	36.814647028434976	39.0	35.0	40.0	32.0	41.0
30	36.52957114523838	39.0	35.0	40.0	31.0	41.0
31	36.318980372606184	38.0	35.0	40.0	31.0	41.0
32	36.15334149786675	39.0	35.0	40.0	30.0	41.0
33	35.96346551929684	39.0	35.0	40.0	29.0	41.0
34	35.89416452618612	39.0	35.0	40.0	29.0	41.0
35	35.70725090044688	39.0	35.0	40.0	27.0	41.0
36	35.45420455407558	39.0	35.0	40.0	25.0	41.0
37	35.41943303456921	39.0	35.0	40.0	25.0	41.0
38	35.35365073860126	39.0	35.0	40.0	24.0	41.0
39	35.28729403519419	39.0	35.0	40.0	24.0	41.0
40	35.2516049088354	39.0	35.0	40.0	24.0	41.0
41	35.04598872138349	38.0	35.0	40.0	23.0	41.0
42	35.03339872185313	38.0	35.0	40.0	23.0	41.0
43	34.93751964365063	38.0	35.0	40.0	23.0	41.0
44	34.87919380651501	38.0	35.0	40.0	22.0	41.0
45	34.89416452618612	38.0	35.0	40.0	23.0	41.0
46	34.74390821041376	38.0	34.0	40.0	23.0	41.0
47	34.59401676980712	38.0	34.0	40.0	22.0	41.0
48	34.569812179605286	38.0	34.0	40.0	22.0	41.0
49	34.55848659896607	38.0	34.0	40.0	22.0	41.0
50	34.49616881075984	38.0	34.0	40.0	21.0	41.0
51	33.52243259744154	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	7.0
10	5.0
11	8.0
12	6.0
13	12.0
14	14.0
15	18.0
16	34.0
17	87.0
18	182.0
19	264.0
20	466.0
21	752.0
22	1227.0
23	1727.0
24	2429.0
25	3359.0
26	4622.0
27	5078.0
28	5293.0
29	5254.0
30	5880.0
31	6829.0
32	8457.0
33	11572.0
34	17534.0
35	23357.0
36	29080.0
37	38801.0
38	58697.0
39	45748.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.37382002622766	24.941204521561954	24.57921945615537	18.105755996055013
2	37.3856152481693	23.966518187762592	23.061555524246135	15.58631103982197
3	26.498246070366715	23.370434996224805	34.90482538375113	15.226493549657341
4	25.39061512172741	23.634156650662735	33.87631093144321	17.098917296166643
5	19.52118262905201	30.436007759919363	32.13177412420929	17.911035486819333
6	21.151921736083263	31.35469839996821	34.357151372617025	13.136228491331506
7	65.34155566875116	7.295335739341851	21.511377963707563	5.851730628199432
8	62.560556633322136	7.050399737000871	22.608893561217744	7.7801500684592515
9	57.0960994483521	9.237483156134058	24.344760067483843	9.32165732803
10	31.068217205489745	23.00519856795529	31.57217844924442	14.354405777310545
11	21.161675824672063	21.859635052581762	36.070258338842585	20.908430783903587
12	20.68408674636118	19.303341317235475	41.742080221959704	18.270491714443637
13	20.69058947208705	21.461523733142588	42.52421362176534	15.323673173005018
14	14.936038467235294	25.803176942779626	40.56653191573913	18.694252674245952
15	12.76376681225547	24.19483611324858	44.226843974321454	18.81455310017449
16	13.124306827500751	24.86606191317416	41.295198459576525	20.71443279974856
17	13.141286166896068	25.2107063766451	36.69560379614678	24.952403660312058
18	13.242800940727657	25.44155313991337	41.61094191982139	19.704703999537585
19	15.02599283977645	26.226215377501294	39.11714660395149	19.630645178770767
20	15.556687511515243	28.348994064456463	38.36246915721062	17.731849266817672
21	14.354767039850872	27.203069286542608	40.58531756783607	17.856846105770448
22	12.4841496060432	26.31147333701821	36.28737712557847	24.916999931360117
23	13.502909969762324	25.360269068340035	37.77975267966489	23.357068282232746
24	15.756826958855811	24.30176982518506	37.127673794376584	22.813729421582547
25	13.581665203553378	27.140932129606547	34.7942790464114	24.483123620428675
26	14.419433034569213	27.73665405860401	36.72956247493741	21.114350431889367
27	16.812074839147854	25.488156007615416	36.4633119827172	21.236457170519532
28	13.123584302420097	24.156903546514357	35.93442362368004	26.785088527385508
29	17.465598774597463	21.766068054637348	36.63093780142843	24.137395369336758
30	18.762531294367555	24.213983027885856	33.00530694671739	24.018178731029202
31	15.992370135148315	27.34865809029396	31.18382121839404	25.47515055616368
32	16.53534773325819	25.750432611892037	31.91140397461047	25.8028156802393
33	16.22285563587626	24.62726737401872	33.26505471321173	25.88482227689329
34	15.844252493614686	22.100958429519483	35.003811319800434	27.050977757065393
35	18.132489424039132	21.443821868666614	32.12418761086244	28.29950109643181
36	16.969946569270284	25.387725021404805	31.018724237465094	26.623604171859817
37	15.096800297680332	23.767823790583332	34.39219383902864	26.7431820727077
38	14.496743218198963	24.219040703450418	34.189164291365465	27.09505178698516
39	17.92404093827107	22.2628040475855	38.25698049543545	21.55617451870798
40	19.19749139291998	20.22781215792953	39.2175775901621	21.357118858988393
41	16.546908134548623	24.205673989458358	30.84315064286669	28.40426723312633
42	18.28024580303244	25.267063332935948	30.978624095488914	25.474066768542702
43	19.92724172437836	22.4466866806114	32.75964841929576	24.866423175714488
44	18.14983002597478	21.19166061551912	32.612614565383105	28.045894793123004
45	20.955033651605632	17.945716690690624	33.55695484579508	27.542294811908658
46	21.102428768058612	20.134245159985113	35.02223570935706	23.741090362599213
47	15.713475454016699	22.34733948202177	37.83502584833476	24.104159215626773
48	15.878933697485975	20.634955040876857	36.64466577796082	26.841445483676353
49	18.92546070005455	18.702922975213777	38.21760287853992	24.15401344619175
50	17.00679534838353	17.116619160642614	37.871874627448	28.00471086352585
51	16.46454027535431	17.17803379249802	33.079365767484205	33.278060164663465
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1767.0
1	2926.0
2	4085.0
3	6609.5
4	9134.0
5	6076.0
6	3018.0
7	3014.0
8	3010.0
9	3229.0
10	3448.0
11	3268.0
12	3088.0
13	2959.0
14	2830.0
15	2761.0
16	2692.0
17	2538.0
18	2384.0
19	2370.0
20	2356.0
21	2600.0
22	2844.0
23	2647.5
24	2451.0
25	2963.0
26	3414.5
27	3354.0
28	3355.5
29	3357.0
30	3336.0
31	3315.0
32	3636.5
33	3958.0
34	4127.5
35	4297.0
36	4516.5
37	4736.0
38	4880.5
39	5025.0
40	6208.0
41	7391.0
42	9351.0
43	11311.0
44	14028.0
45	16745.0
46	22165.5
47	27586.0
48	33055.0
49	38524.0
50	37787.0
51	37050.0
52	29888.5
53	22727.0
54	17397.0
55	12067.0
56	9387.5
57	6708.0
58	6093.5
59	5479.0
60	5204.5
61	4930.0
62	4366.5
63	3803.0
64	3334.5
65	2866.0
66	2381.0
67	1896.0
68	1504.0
69	1112.0
70	962.0
71	812.0
72	660.5
73	509.0
74	398.5
75	254.5
76	221.0
77	155.0
78	89.0
79	62.0
80	35.0
81	27.5
82	20.0
83	11.0
84	2.0
85	3.5
86	5.0
87	2.5
88	0.0
89	3.0
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	276807.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.716011327069175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.33176333705819	32.34387948686781
2	11.237358428447008	10.049796935455454
3	4.93872106269067	6.625197209415335
4	2.9089991427848205	5.203153544768023
5	1.992133237381073	4.454012620388152
6	1.453741211457849	3.900330508690586
7	1.113187885248736	3.4844125460157067
8	0.8993295238277289	3.2171543339398765
9	0.6422555727931886	2.5847196721104173
>10	2.3413290542229634	15.96551458031858
>50	0.07915959408390406	2.4562607720289193
>100	0.05304508881911098	4.649050993262284
>500	0.006528626316198273	1.9720150367024631
>1k	0.0016321565790495683	1.2162705620520557
>5k	8.160782895247842E-4	1.8782311979843778
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5147	1.859418295057567	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	1818	0.6567752983125427	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCC	1515	0.5473127485937855	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	831	0.3002091710108487	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	815	0.2944289703656338	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	801	0.28937129480107077	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	678	0.24493600234098126	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	619	0.2236215124617513	No Hit
TCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	588	0.2124223737116475	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	548	0.1979718720986102	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	524	0.18930157113078788	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG	480	0.1734060193564469	No Hit
ACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG	427	0.15425910471917256	No Hit
CTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGCT	408	0.14739511645297987	No Hit
ACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	381	0.13764102786417973	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	360	0.13005451451733518	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGC	344	0.12427431387212029	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	332	0.1199391633882091	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	326	0.11777158814625353	No Hit
GGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG	313	0.11307517512201641	No Hit
GCCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG	294	0.10621118685582374	No Hit
AGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG	292	0.10548866177517188	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTC	290	0.10476613669452	No Hit
CGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG	290	0.10476613669452	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15389784217884664	0.0
2	0.0	0.0	0.0	1.5386171592481404	0.0
3	0.0	0.0	0.0	2.4294905836918863	0.0
4	0.0	0.0	0.0	3.7224492155183935	0.0
5	0.0	0.0	0.0	7.207910204582976	0.0
6	0.0	0.0	0.0	9.28516981145708	0.0
7	0.0	0.0	0.0	11.338586090669672	0.0
8	0.0	0.0	0.0	14.208816973559195	0.0
9	0.0	0.0	0.0	15.329092111109906	0.0
10	0.0	0.0	0.0	18.243035761378867	0.0
11	0.0	0.0	0.0	23.5333644019118	0.0
12	0.0	0.0	0.0	27.03074705480714	0.0
13	0.0	0.0	0.0	28.38584284356971	0.0
14	0.0	0.0	0.0	28.930626754381212	0.0
15	0.0	0.0	0.0	29.954083531124574	0.0
16	0.0	0.0	0.0	32.103595646063866	0.0
17	0.0	0.0	0.0	34.5406727431026	0.0
18	0.0	0.0	0.0	37.1529621721994	0.0
19	0.0	0.0	0.0	38.704584782899275	0.0
20	0.0	0.0	0.0	40.19407023666309	0.0
21	0.0	0.0	0.0	41.944025982001904	0.0
22	0.0	0.0	0.0	43.61847785641259	0.0
23	0.0	0.0	0.0	45.095319121265	0.0
24	0.0	0.0	0.0	46.21451047119473	0.0
25	0.0	0.0	0.0	46.99339250813744	0.0
26	0.0	0.0	0.0	47.69243552366812	0.0
27	0.0	0.0	0.0	48.21445989443909	0.0
28	0.0	0.0	0.0	48.76791410621841	0.0
29	0.0	0.0	0.0	49.27657176299732	0.0
30	0.0	0.0	0.0	49.74910316574364	0.0
31	0.0	0.0	0.0	50.19164977764291	0.0
32	0.0	0.0	0.0	50.56411145671894	0.0
33	0.0	0.0	0.0	50.89394415603652	0.0
34	0.0	0.0	0.0	51.196682164829646	0.0
35	0.0	0.0	0.0	51.530488752090804	0.0
36	0.0	0.0	0.0	51.84767726249697	0.0
37	0.0	0.0	0.0	52.14391254556424	0.0
38	0.0	0.0	0.0	52.416665763510316	0.0
39	0.0	0.0	0.0	52.67460721730303	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGG	25	3.8831087E-5	45.0	24
ATGGGTC	25	3.8831087E-5	45.0	5
ATGGGTA	35	1.2074634E-7	45.0	5
CGGGTGA	20	7.024E-4	45.0	6
AACGTAG	20	7.024E-4	45.0	1
AGGTACG	25	3.8831087E-5	45.0	1
AACCGAG	20	7.024E-4	45.0	1
GCCTTCG	25	3.8831087E-5	45.0	23
ACCGGGA	20	7.024E-4	45.0	4
TTGTGCG	25	3.8831087E-5	45.0	1
GCGAAGG	40	6.7830115E-9	45.0	2
TATGGGT	35	1.2074634E-7	45.0	4
TCGGGTA	25	3.8831087E-5	45.0	27
CCGGAAT	20	7.024E-4	45.0	42
AATGGCC	20	7.024E-4	45.0	19
CCTAAGG	20	7.024E-4	45.0	2
ACGGGTA	25	3.8831087E-5	45.0	5
GCCATCG	25	3.8831087E-5	45.0	22
AACGGGC	25	3.8831087E-5	45.0	4
ACGATAG	25	3.8831087E-5	45.0	1
>>END_MODULE