FastQCFastQC Report
Sat 14 Jan 2017
SRR2934822.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934822.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194612
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33611.7270260826670503No Hit
GCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC15020.7717920785974143TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC13300.683411094896512TruSeq Adapter, Index 21 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11500.5909193677676607No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCC9820.504593755780733No Hit
CTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGCT9530.489692310854418Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC7620.391548311512137TruSeq Adapter, Index 21 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC4880.2507553491048856No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4410.22660473146568558No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.20707870018292807No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.18035886790125993No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCG3370.17316506690234929No Hit
GCAATCGGGAAATTTTAAACATTTAAATTTTTTCATAATCTTTCATAAAGA3240.16648510883193224No Hit
GCCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTG2860.14695907754917475TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGC2410.12383614576696195No Hit
CGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTG2230.11458697305407683No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.10790701498365979No Hit
AGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTG2020.10379627155571085No Hit
GATAATGGGATGCATCTCTTTTTTTAAAAAAGATTTTACTTTTAAGTACTG2020.10379627155571085No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATC351.2049895E-745.0000046
CGGGACT351.2049895E-745.0000046
GTGCATG207.018835E-445.01
CGTATGG207.018835E-445.02
AATCAGG207.018835E-445.02
AATCAAT207.018835E-445.030
ACGTTGG207.018835E-445.02
TAGACGG253.8788305E-545.02
GTTTAGG207.018835E-445.02
TTAGATG253.8788305E-545.01
GAGTACG253.8788305E-545.01
TGAACGG207.018835E-445.02
TGTATTG207.018835E-445.01
GATTTAC207.018835E-445.09
ACGGGCA253.8788305E-545.05
CGTTATT750.045.01
TATTGTG207.018835E-445.015
AACGGGC207.018835E-445.04
CAGGGCA207.018835E-445.05
TACGACG253.8788305E-545.01
Produced by FastQC (version 0.11.5)