FastQCFastQC Report
Sat 14 Jan 2017
SRR2934821.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934821.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196633
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47392.410073588868603No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15490.787761972812295No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC14630.7440256721913412No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC12320.6265479344769189RNA PCR Primer, Index 14 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC11980.6092568388825883Illumina PCR Primer Index 7 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC10740.5461951961267946No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCG10600.5390753332350114No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGC10090.5131386898435156No Hit
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT6670.33921061062995533Illumina PCR Primer Index 7 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC5380.27360615969852464RNA PCR Primer, Index 14 (95% over 24bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCG4680.2380068452396088No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4580.2329212288883351No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.20291609241582034No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.18003081883508873No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGC3510.1785051339297066No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.16680821632177711No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.16477396978126765No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTCCATAT2550.12968321695747917No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.12917465532235178No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCT2540.12917465532235178No Hit
ACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC2500.12714040878184232RNA PCR Primer, Index 14 (95% over 24bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGT2300.11696917607929493No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTC2250.11442636790365808No Hit
CGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG2120.10781506664700227Illumina PCR Primer Index 7 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG2080.10578082010649281Illumina PCR Primer Index 7 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG2070.10527225847136543RNA PCR Primer, Index 14 (95% over 23bp)
AAGATAGGGAAATTTTTTCATAATCTTTCATAAAGAAGTTTAATAATAGGT2020.10272945029572858No Hit
TGAGCCTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1970.10018664212009173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG551.8189894E-1245.0000041
ATGGGTA207.0190104E-445.05
CTATGGT207.0190104E-445.013
CCGGGTG207.0190104E-445.031
GTACCCC207.0190104E-445.014
TTGTGCG207.0190104E-445.01
TGACGAG207.0190104E-445.02
CGTTATT1000.045.01
AGAGTAC253.8789767E-545.011
ATATCTA253.8789767E-545.010
TGCGAAG253.8789767E-545.01
TTGACGA207.0190104E-445.01
TCAGAGG207.0190104E-445.01
AGTCTTG207.0190104E-445.01
AATCTAT406.7648216E-945.021
CATACGG207.0190104E-445.02
GCGATGA207.0190104E-445.09
AGTTATG207.0190104E-445.01
CGACTGG253.8789767E-545.02
TATACGG207.0190104E-445.02
Produced by FastQC (version 0.11.5)