FastQCFastQC Report
Sat 14 Jan 2017
SRR2934817.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934817.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169950
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45552.6802000588408355No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCC13960.8214180641365106No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10720.6307737569873492No Hit
CCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC10470.6160635481023831Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC9890.5819358634892615Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGCT6290.3701088555457488Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG6210.36540158870255957No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGC5700.3353927625772286No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC5510.32421300382465434No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5420.3189173286260665No Hit
TCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC5120.30126507796410706Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG4140.2436010591350397No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3830.22536040011768166No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.21888790820829657No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.20476610767872902No Hit
TTAGAAAGGGACGTACTTTTAGTTTTAGAAAACTTTTATATTTATGTGTCT3440.20241247425713446No Hit
ACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC3410.20064724919093851Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGC3100.18240659017358046No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3030.17828773168578993No Hit
ACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTG2300.13533392174168873No Hit
GTTCAGAAAAATAAAAAAAGAAAAGAAAGAAGAAGAAGAAGAAGAAGAAGA2060.12121212121212122No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGT1860.10944395410414828No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTG1850.10885554574874964No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCAAAGGT1780.1047366872609591No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAAG351.2037708E-745.0000041
CGTTATT1150.045.0000041
TAGGGTT600.045.0000045
TAGGGCG302.1545584E-645.0000045
CGTAAGG302.1545584E-645.0000042
TAAGGGT650.045.0000044
TGGGACC650.045.0000046
CGTGGGA207.0163136E-445.04
TAATAGT207.0163136E-445.014
GGGCTCA253.8767426E-545.07
CCGATGC207.0163136E-445.011
CCGATGA253.8767426E-545.018
GCGAAGG253.8767426E-545.02
GGCCGAT207.0163136E-445.08
AGGGTCC502.1827873E-1145.06
TAACGCG207.0163136E-445.01
ACGGGAC253.8767426E-545.05
CATTAGG253.8767426E-545.02
GCCGATG207.0163136E-445.09
GCGGGTA207.0163136E-445.05
Produced by FastQC (version 0.11.5)