##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934817.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	169950
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.693704030597235	31.0	31.0	34.0	30.0	34.0
2	31.811091497499266	33.0	31.0	34.0	30.0	34.0
3	31.811450426596057	33.0	31.0	34.0	30.0	34.0
4	35.58702559576346	37.0	35.0	37.0	33.0	37.0
5	33.15783465725213	37.0	35.0	37.0	28.0	37.0
6	34.14118270079435	37.0	35.0	37.0	28.0	37.0
7	35.43822889085025	37.0	35.0	37.0	32.0	37.0
8	35.44460723742277	37.0	35.0	37.0	33.0	37.0
9	37.50062371285672	39.0	37.0	39.0	35.0	39.0
10	36.78803765813475	39.0	37.0	39.0	32.0	39.0
11	36.65586348926155	39.0	37.0	39.0	32.0	39.0
12	36.99320388349515	39.0	37.0	39.0	33.0	39.0
13	36.89582818476023	39.0	37.0	39.0	33.0	39.0
14	37.89153868784937	40.0	37.0	41.0	33.0	41.0
15	38.068584877905266	40.0	37.0	41.0	33.0	41.0
16	38.08616063548102	40.0	37.0	41.0	33.0	41.0
17	38.02225948808473	40.0	37.0	41.0	33.0	41.0
18	37.88613121506325	39.0	37.0	41.0	33.0	41.0
19	37.64484260076493	39.0	36.0	40.0	33.0	41.0
20	37.415639894086496	39.0	35.0	40.0	33.0	41.0
21	37.35056781406296	39.0	35.0	40.0	33.0	41.0
22	37.35059723448073	39.0	35.0	40.0	33.0	41.0
23	37.06978523095028	39.0	35.0	40.0	32.0	41.0
24	36.81192703736393	39.0	35.0	40.0	32.0	41.0
25	36.651609296852016	38.0	35.0	40.0	31.0	41.0
26	36.88853192115328	38.0	35.0	40.0	32.0	41.0
27	36.74413651073845	38.0	35.0	40.0	32.0	41.0
28	36.82984995586937	39.0	35.0	40.0	32.0	41.0
29	36.874927919976464	39.0	35.0	40.0	33.0	41.0
30	36.54165931156223	38.0	35.0	40.0	31.0	41.0
31	36.40239482200647	38.0	35.0	40.0	31.0	41.0
32	36.11735804648426	38.0	35.0	40.0	30.0	41.0
33	35.837575757575756	38.0	35.0	40.0	30.0	41.0
34	35.609349808767284	38.0	35.0	40.0	27.0	41.0
35	35.39172109443954	38.0	35.0	40.0	25.0	41.0
36	35.18421888790821	38.0	35.0	40.0	25.0	41.0
37	35.057281553398056	38.0	35.0	40.0	24.0	41.0
38	34.98247719917623	38.0	35.0	40.0	24.0	41.0
39	34.842447778758455	38.0	35.0	40.0	23.0	41.0
40	34.75279788172992	38.0	34.0	40.0	23.0	41.0
41	34.76504265960577	38.0	35.0	40.0	23.0	41.0
42	34.8028773168579	38.0	35.0	40.0	23.0	41.0
43	34.56904972050603	38.0	34.0	40.0	22.0	41.0
44	34.4753456899088	38.0	34.0	40.0	22.0	41.0
45	34.51612238893792	38.0	34.0	40.0	22.0	41.0
46	34.46578405413357	38.0	34.0	40.0	22.0	41.0
47	34.418199470432484	38.0	34.0	40.0	22.0	41.0
48	34.39295675198588	38.0	34.0	40.0	22.0	41.0
49	34.36327743453957	38.0	34.0	40.0	21.0	41.0
50	34.219782288908505	37.0	34.0	40.0	20.0	41.0
51	33.370126507796414	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	6.0
12	6.0
13	5.0
14	10.0
15	11.0
16	26.0
17	50.0
18	92.0
19	155.0
20	289.0
21	480.0
22	696.0
23	976.0
24	1405.0
25	2186.0
26	2859.0
27	3242.0
28	3283.0
29	3209.0
30	3390.0
31	4035.0
32	5218.0
33	6945.0
34	10937.0
35	16021.0
36	19086.0
37	23949.0
38	34021.0
39	27355.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.656369520447186	22.533686378346573	24.559576345984112	19.250367755222126
2	36.63724624889673	23.63577522800824	25.049132097675788	14.677846425419242
3	24.241247425713443	24.732568402471315	37.629891144454255	13.39629302736099
4	23.345101500441306	24.5636952044719	38.20653133274492	13.884671962341866
5	19.35686966754928	32.29008531921153	33.53692262430126	14.816122388937922
6	20.055310385407473	32.39894086496029	35.11797587525743	12.427772874374817
7	65.72697852309503	6.880258899676376	22.37422771403354	5.0185348631950575
8	65.88761400411886	6.498381877022654	20.767872903795233	6.846131215063254
9	61.41688731979994	7.33921741688732	22.96675492791998	8.277140335392764
10	30.545454545454547	23.216240070609004	31.66990291262136	14.568402471315093
11	20.620770814945573	26.297146219476314	35.37746395998823	17.70461900558988
12	20.244777875845838	21.02677258017064	40.80906148867314	17.919388055310385
13	18.951456310679614	24.80553103854075	41.47337452191821	14.769638128861429
14	13.964107090320683	28.475433951162106	39.067372756693146	18.493086201824067
15	11.696969696969697	26.4771991762283	44.05648720211827	17.76934392468373
16	13.0867902324213	26.620770814945573	39.81818181818182	20.47425713445131
17	13.267431597528685	26.36834363047955	37.576934392468374	22.78729037952339
18	13.686378346572523	26.27007943512798	41.61753456899088	18.42600764930862
19	14.927331568108269	27.76051779935275	38.313033245072084	18.999117387466903
20	15.622830244189467	29.255075022065313	38.79729332156517	16.324801412180054
21	14.21359223300971	29.477493380406	39.36863783465725	16.940276551927038
22	12.8690791409238	27.693439246837304	35.99293909973522	23.444542512503677
23	13.114445425125037	27.323330391291556	37.458664313033246	22.103559870550164
24	15.858193586348927	25.949396881435717	37.0844365989997	21.107972933215652
25	13.519270373639305	28.594292438952635	34.69079140923801	23.19564577817005
26	14.280082377169755	28.234186525448663	38.19711679905854	19.288614298323036
27	15.411591644601351	28.393056781406294	37.48631950573698	18.709032068255368
28	12.18652544866137	26.85142689026184	37.591644601353345	23.37040305972345
29	15.84819064430715	26.786701971167993	36.47366872609591	20.89143865842895
30	16.681965283907033	30.185937040305973	34.61253309796999	18.519564577817004
31	15.45278022947926	32.7819947043248	30.057664018829065	21.707561047366873
32	18.94851426890262	32.29361576934392	28.802588996763756	19.9552809649897
33	17.85701676963813	30.7825831126802	29.77758164165931	21.582818476022357
34	17.194468961459254	29.870550161812297	30.435422182994998	22.49955869373345
35	20.482494851426893	28.970285378052367	29.764048249485143	20.783171521035598
36	20.731979994115918	30.119446896145924	30.581935863489264	18.566637246248895
37	16.96498970285378	31.393939393939398	32.16416593115623	19.476904972050605
38	17.5204471903501	34.159458664313036	27.06913798175934	21.250956163577523
39	19.651073845248604	33.492203589290966	28.150044130626657	18.706678434833773
40	21.79523389232127	30.437187408061195	29.8699617534569	17.897616946160635
41	23.014416004707268	28.319505736981466	27.76051779935275	20.905560458958515
42	25.10856134157105	24.484848484848484	30.74257134451309	19.664018829067373
43	22.83083259782289	25.70520741394528	31.03206825536923	20.431891732862606
44	18.830832597822887	28.236540158870255	28.830832597822887	24.101794645483963
45	20.912032950867903	27.585172109443956	28.232421300382466	23.270373639305678
46	22.929096793174462	28.626066490144158	30.096498970285378	18.348337746396
47	18.3477493380406	29.0438364224772	33.74404236540159	18.864371874080614
48	20.179464548396588	25.297440423654017	32.637834657252135	21.885260370697264
49	21.515739923506914	24.38246543100912	33.73227419829362	20.36952044719035
50	19.593409826419535	24.141218005295677	32.310679611650485	23.954692556634306
51	18.689614592527214	23.742277140335393	29.029714621947633	28.538393645189764
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1421.0
1	1989.0
2	2557.0
3	4744.0
4	6931.0
5	4366.5
6	1802.0
7	1857.5
8	1913.0
9	2133.0
10	2353.0
11	2646.5
12	2940.0
13	2774.0
14	2608.0
15	2476.0
16	2344.0
17	2179.5
18	2015.0
19	1908.5
20	1802.0
21	1836.5
22	1871.0
23	1837.5
24	1804.0
25	1944.5
26	1688.0
27	1291.0
28	1595.5
29	1900.0
30	2056.5
31	2213.0
32	2363.5
33	2514.0
34	2714.5
35	2915.0
36	3291.0
37	3667.0
38	3936.5
39	4206.0
40	5049.0
41	5892.0
42	7363.5
43	8835.0
44	10346.0
45	11857.0
46	15145.0
47	18433.0
48	20011.0
49	21589.0
50	20756.0
51	19923.0
52	15666.0
53	11409.0
54	8924.0
55	6439.0
56	4998.0
57	3557.0
58	3127.0
59	2697.0
60	2543.5
61	2390.0
62	1999.0
63	1608.0
64	1334.5
65	1061.0
66	793.5
67	526.0
68	373.0
69	220.0
70	200.5
71	181.0
72	131.0
73	81.0
74	59.5
75	36.0
76	34.0
77	27.5
78	21.0
79	13.0
80	5.0
81	2.5
82	0.0
83	0.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	169950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.983818770226534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.32962720732505	33.4363047955281
2	10.409417920209286	9.36510738452486
3	4.508829300196206	6.084730803177405
4	2.562459123610203	4.6107678729037955
5	1.6991497710922172	3.8217122683142097
6	1.2465663832570308	3.3645189761694616
7	1.0202746893394377	3.212709620476611
8	0.8031393067364291	2.8902618417181523
9	0.5977763243950295	2.4201235657546336
>10	2.712884238064094	18.224183583406884
>50	0.061478090255068674	1.9717563989408649
>100	0.034009156311314584	3.25213298028832
>500	0.009156311314584695	2.597234480729626
>1k	0.005232177894048397	4.7484554280670785
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4555	2.6802000588408355	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCC	1396	0.8214180641365106	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1072	0.6307737569873492	No Hit
CCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	1047	0.6160635481023831	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	989	0.5819358634892615	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGCT	629	0.3701088555457488	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG	621	0.36540158870255957	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGC	570	0.3353927625772286	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC	551	0.32421300382465434	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	542	0.3189173286260665	No Hit
TCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	512	0.30126507796410706	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG	414	0.2436010591350397	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	383	0.22536040011768166	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	372	0.21888790820829657	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	348	0.20476610767872902	No Hit
TTAGAAAGGGACGTACTTTTAGTTTTAGAAAACTTTTATATTTATGTGTCT	344	0.20241247425713446	No Hit
ACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	341	0.20064724919093851	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGC	310	0.18240659017358046	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	303	0.17828773168578993	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTG	230	0.13533392174168873	No Hit
GTTCAGAAAAATAAAAAAAGAAAAGAAAGAAGAAGAAGAAGAAGAAGAAGA	206	0.12121212121212122	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGT	186	0.10944395410414828	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTG	185	0.10885554574874964	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCAAAGGT	178	0.1047366872609591	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40129449838187703	0.0
2	0.0	0.0	0.0	2.2218299499852896	0.0
3	0.0	0.0	0.0	3.01735804648426	0.0
4	0.0	0.0	0.0	4.022947925860548	0.0
5	0.0	0.0	0.0	6.80435422182995	0.0
6	0.0	0.0	0.0	8.21300382465431	0.0
7	0.0	0.0	0.0	9.857605177993527	0.0
8	0.0	0.0	0.0	12.254192409532216	0.0
9	0.0	0.0	0.0	13.102677258017064	0.0
10	0.0	0.0	0.0	15.978817299205648	0.0
11	0.0	0.0	0.0	20.258311268020005	0.0
12	0.0	0.0	0.0	23.10856134157105	0.0
13	0.0	0.0	0.0	24.132391879964697	0.0
14	0.0	0.0	0.0	24.4783759929391	0.0
15	0.0	0.0	0.0	25.255075022065313	0.0
16	0.0	0.0	0.0	27.06501912327155	0.0
17	0.0	0.0	0.0	29.055016181229774	0.0
18	0.0	0.0	0.0	31.320388349514563	0.0
19	0.0	0.0	0.0	32.81082671373934	0.0
20	0.0	0.0	0.0	34.00411885848779	0.0
21	0.0	0.0	0.0	35.65931156222418	0.0
22	0.0	0.0	0.0	37.19623418652545	0.0
23	0.0	0.0	0.0	38.68784936746102	0.0
24	0.0	0.0	0.0	39.62165342747867	0.0
25	0.0	0.0	0.0	40.33480435422183	0.0
26	0.0	0.0	0.0	41.03501029714622	0.0
27	0.0	0.0	0.0	41.532803765813476	0.0
28	0.0	0.0	0.0	42.0441306266549	0.0
29	0.0	0.0	0.0	42.55663430420712	0.0
30	0.0	0.0	0.0	43.046778464254196	0.0
31	0.0	0.0	0.0	43.4792586054722	0.0
32	5.884083553986466E-4	0.0	0.0	43.87525742865549	0.0
33	5.884083553986466E-4	0.0	0.0	44.25066195939982	0.0
34	5.884083553986466E-4	0.0	0.0	44.610179464548395	0.0
35	5.884083553986466E-4	0.0	0.0	44.97205060311857	0.0
36	5.884083553986466E-4	0.0	0.0	45.29214474845543	0.0
37	5.884083553986466E-4	0.0	0.0	45.63165636952045	0.0
38	5.884083553986466E-4	0.0	0.0	45.955280964989704	0.0
39	5.884083553986466E-4	0.0	0.0	46.270079435127975	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTAAG	35	1.2037708E-7	45.000004	1
CGTTATT	115	0.0	45.000004	1
TAGGGTT	60	0.0	45.000004	5
TAGGGCG	30	2.1545584E-6	45.000004	5
CGTAAGG	30	2.1545584E-6	45.000004	2
TAAGGGT	65	0.0	45.000004	4
TGGGACC	65	0.0	45.000004	6
CGTGGGA	20	7.0163136E-4	45.0	4
TAATAGT	20	7.0163136E-4	45.0	14
GGGCTCA	25	3.8767426E-5	45.0	7
CCGATGC	20	7.0163136E-4	45.0	11
CCGATGA	25	3.8767426E-5	45.0	18
GCGAAGG	25	3.8767426E-5	45.0	2
GGCCGAT	20	7.0163136E-4	45.0	8
AGGGTCC	50	2.1827873E-11	45.0	6
TAACGCG	20	7.0163136E-4	45.0	1
ACGGGAC	25	3.8767426E-5	45.0	5
CATTAGG	25	3.8767426E-5	45.0	2
GCCGATG	20	7.0163136E-4	45.0	9
GCGGGTA	20	7.0163136E-4	45.0	5
>>END_MODULE