FastQCFastQC Report
Sat 14 Jan 2017
SRR2934815.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934815.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199601
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38531.9303510503454393No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCC13830.6928823001888769No Hit
CCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC13180.6603173330794936TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC11760.589175404932841TruSeq Adapter, Index 14 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9970.49949649550853953No Hit
CTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGCT9260.4639255314352132TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC6580.3296576670457563TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCG4490.22494877280173947No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4440.2224437753317869No Hit
ACTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC4260.21342578443995772TruSeq Adapter, Index 21 (95% over 22bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.19989879810221392No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.19939779860822343No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.180359817836584No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGC3160.1583158401010015No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.14879684971518178No Hit
ACCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG2860.14328585528128615TruSeq Adapter, Index 21 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.13276486590748543No Hit
AGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG2600.13025986843753287TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTC2470.12374687501565623No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGT2410.12074087805171317No Hit
CGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG2270.11372688513584601TruSeq Adapter, Index 21 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG2230.11172288715988396No Hit
GGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG2080.1042078947500263No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG207.019268E-445.0000042
CGAAAGG207.019268E-445.0000042
TACGGGA850.045.0000044
CTGTTAA207.019268E-445.00000436
GCTAACG207.019268E-445.0000041
TGCGTGG207.019268E-445.0000042
GACTAGG207.019268E-445.0000042
ATGGGCA900.045.05
GTACAGG351.2051896E-745.02
TCGATAG351.2051896E-745.01
TCAGGGT253.8791914E-545.04
CGTTATT900.045.01
CGTTAGG253.8791914E-545.02
CTAACGG253.8791914E-545.02
GGGACAC351.2051896E-745.07
CGGGATG351.2051896E-745.06
GACGGGA453.8198777E-1045.04
AGGGCCC351.2051896E-745.06
GCGTAAG253.8791914E-545.01
GTACGGG453.8198777E-1045.03
Produced by FastQC (version 0.11.5)