##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934815.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	199601
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70378905917305	31.0	31.0	34.0	30.0	34.0
2	31.8131221787466	33.0	31.0	34.0	30.0	34.0
3	31.804815607136238	33.0	31.0	34.0	30.0	34.0
4	35.60723142669626	37.0	35.0	37.0	33.0	37.0
5	33.187604270519685	37.0	35.0	37.0	28.0	37.0
6	34.161802796579174	37.0	35.0	37.0	28.0	37.0
7	35.42986758583374	37.0	35.0	37.0	32.0	37.0
8	35.42146582431952	37.0	35.0	37.0	33.0	37.0
9	37.4360398995997	39.0	37.0	39.0	34.0	39.0
10	36.80853302338165	39.0	37.0	39.0	32.0	39.0
11	36.65944058396501	39.0	37.0	39.0	32.0	39.0
12	36.98787080225049	39.0	37.0	39.0	33.0	39.0
13	36.96115249923598	39.0	37.0	39.0	33.0	39.0
14	37.887159883968515	40.0	37.0	41.0	33.0	41.0
15	38.072514666760185	40.0	37.0	41.0	33.0	41.0
16	38.041372538213736	40.0	37.0	41.0	33.0	41.0
17	38.03795572166472	40.0	37.0	41.0	33.0	41.0
18	37.91334211752446	39.0	37.0	41.0	33.0	41.0
19	37.72541219733368	39.0	37.0	41.0	33.0	41.0
20	37.53807345654581	39.0	35.0	40.0	33.0	41.0
21	37.455228180219535	39.0	35.0	40.0	33.0	41.0
22	37.421049994739505	39.0	35.0	40.0	33.0	41.0
23	37.1961062319327	39.0	35.0	40.0	33.0	41.0
24	36.930521390173396	39.0	35.0	40.0	32.0	41.0
25	36.72225590052154	38.0	35.0	40.0	31.0	41.0
26	36.974489105766004	39.0	35.0	40.0	32.0	41.0
27	36.93642817420754	39.0	35.0	40.0	32.0	41.0
28	36.9611925791955	39.0	35.0	40.0	32.0	41.0
29	37.017214342613514	39.0	35.0	40.0	33.0	41.0
30	36.77877365343861	39.0	35.0	40.0	32.0	41.0
31	36.53376987089243	39.0	35.0	40.0	31.0	41.0
32	36.25108090640828	39.0	35.0	40.0	30.0	41.0
33	36.111617677266146	39.0	35.0	40.0	30.0	41.0
34	35.922740868031724	39.0	35.0	40.0	29.0	41.0
35	35.62784254587903	39.0	35.0	40.0	27.0	41.0
36	35.49990230509867	38.0	35.0	40.0	26.0	41.0
37	35.4318715838097	38.0	35.0	40.0	25.0	41.0
38	35.39877555723669	38.0	35.0	40.0	25.0	41.0
39	35.39079463529742	38.0	35.0	40.0	25.0	41.0
40	35.26395659340384	38.0	35.0	40.0	24.0	41.0
41	35.01946383034153	38.0	35.0	40.0	24.0	41.0
42	35.060490678904415	38.0	35.0	40.0	23.0	41.0
43	35.03393770572292	38.0	35.0	40.0	23.0	41.0
44	35.02103696875266	38.0	35.0	40.0	23.0	41.0
45	35.0464025731334	38.0	35.0	40.0	23.0	41.0
46	34.93058652010762	38.0	35.0	40.0	23.0	41.0
47	34.81196486991548	38.0	35.0	40.0	23.0	41.0
48	34.80411420784465	38.0	35.0	40.0	23.0	41.0
49	34.81975541204704	38.0	34.0	40.0	23.0	41.0
50	34.77868347352969	38.0	34.0	40.0	23.0	41.0
51	33.763282749084425	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	2.0
12	6.0
13	9.0
14	12.0
15	18.0
16	25.0
17	45.0
18	72.0
19	167.0
20	272.0
21	461.0
22	738.0
23	1053.0
24	1558.0
25	2215.0
26	3190.0
27	3657.0
28	3709.0
29	3592.0
30	3692.0
31	4619.0
32	5833.0
33	7914.0
34	12415.0
35	17183.0
36	20875.0
37	28327.0
38	43390.0
39	34535.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.30043937655623	23.960300800096192	24.37262338365038	19.366636439697196
2	37.70171492126793	22.864614906738943	23.972825787445956	15.46084438454717
3	26.51489721995381	22.983852786308688	36.423665212098136	14.077584781639372
4	24.4668112885206	24.687251065876424	34.79742085460494	16.04851679099804
5	18.445298370248647	32.432202243475736	32.919674751128504	16.202824635147117
6	21.465323320023447	31.157158531269886	34.00083165916003	13.376686489546646
7	66.92601740472243	6.552572381901895	21.079052710156763	5.442357503218922
8	65.40698693894319	5.64676529676705	21.3095124773924	7.63673528689736
9	60.37745301877246	7.574110350148547	23.760903001487968	8.287533629591035
10	29.349051357458126	24.24386651369482	31.49683618819545	14.9102459406516
11	19.77044203185355	24.81250093937405	35.17767947054373	20.239377558228668
12	19.073551735712748	21.70931007359683	41.21271937515343	18.004418815536997
13	19.081567727616594	23.216316551520283	41.564922019428764	16.13719370143436
14	14.561049293340215	26.56549816884685	39.61302799084173	19.26042454697121
15	13.130194738503315	24.886147864990658	43.11401245484742	18.86964494165861
16	13.33309953356947	25.74285699971443	40.2007003972926	20.7233430694235
17	13.823077038692192	25.50939123551485	36.63508699856213	24.032444727230825
18	13.793017069052759	25.10358164538254	42.21572036212243	18.887680923442268
19	14.874674976578273	25.83203490964474	39.46072414466861	19.83256596910837
20	15.69531214773473	28.755366957079374	37.87355774770668	17.67576314747922
21	15.083090766078328	26.82251090926398	40.21272438514837	17.88167393950932
22	13.430794434897619	26.65517707827115	36.15763448078918	23.756394006042054
23	13.4583494070671	25.079032670177003	37.83698478464537	23.62563313811053
24	16.35262348385028	24.470819284472523	37.209232418675256	21.96732481300194
25	13.1572487111788	27.394652331401144	35.2007254472673	24.247373510152755
26	12.532001342678644	29.741333961252696	36.782380849795345	20.944283846273315
27	14.723373129393138	27.64364907991443	36.7949058371451	20.838071953547328
28	11.520984363805793	28.026913692817168	36.095009544040366	24.35709239933668
29	13.297027570002154	27.542948181622336	36.966247664089856	22.193776584285647
30	18.610127203771523	27.383129343039364	32.51486715998417	21.491876293204946
31	16.444306391250546	33.01436365549271	30.170690527602567	20.37063942565418
32	16.22086061693078	29.772395929880112	32.47929619591084	21.527447257278272
33	21.023942765817807	26.396661339372045	30.424196271561765	22.155199623248382
34	18.648203165314804	26.52942620527953	32.042424637151115	22.779945992254547
35	17.165745662596883	27.621104102684857	32.37358530267884	22.83956493203942
36	21.690272092825186	28.196752521279954	28.854063857395506	21.258911528499354
37	18.73187008081122	28.83151888016593	33.31446235239303	19.122148686629824
38	18.873151938116543	27.671204052083908	33.03340163626435	20.4222423735352
39	25.81950992229498	23.69527206777521	31.750842931648638	18.73437507828117
40	25.61660512722882	23.138661629951756	32.505849169092336	18.738884073727085
41	20.749896042605	25.976322763914006	30.425198270549746	22.84858292293125
42	22.381651394532092	25.30999343690663	31.206757481174947	21.101597687386338
43	22.228345549370996	24.218315539501305	32.43721223841564	21.11612667271206
44	20.251902545578428	24.81250093937405	29.857064844364505	25.078531670683013
45	23.454792310659766	22.452292323184754	29.80846789344743	24.284447472708052
46	24.367112389216487	22.96782080250099	32.590017084082746	20.07504972419978
47	19.640182163416014	24.653684099779056	35.856533784900876	19.849599951904047
48	19.74288705968407	21.800992980997087	35.981783658398506	22.474336300920335
49	23.386155379983066	19.80150400048096	36.59600903803087	20.216331581505102
50	22.01842676138897	18.26894654836399	35.10102654796319	24.61160014228386
51	20.083566715597616	18.469847345454184	31.93921874138907	29.50736719755913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1604.0
1	2100.5
2	2597.0
3	4676.0
4	6755.0
5	4471.0
6	2187.0
7	2190.0
8	2193.0
9	2216.5
10	2240.0
11	2333.0
12	2426.0
13	2358.5
14	2291.0
15	2218.5
16	2146.0
17	2133.5
18	2121.0
19	1886.5
20	1652.0
21	1649.0
22	1646.0
23	1600.5
24	1555.0
25	1725.5
26	2017.5
27	2139.0
28	2212.5
29	2286.0
30	2454.5
31	2623.0
32	2966.5
33	3310.0
34	3453.5
35	3597.0
36	4043.0
37	4489.0
38	4906.0
39	5323.0
40	6235.0
41	7147.0
42	8769.0
43	10391.0
44	12131.0
45	13871.0
46	16924.5
47	19978.0
48	23606.5
49	27235.0
50	26154.5
51	25074.0
52	19660.0
53	14246.0
54	10991.0
55	7736.0
56	6104.0
57	4472.0
58	3987.0
59	3502.0
60	3244.0
61	2986.0
62	2523.0
63	2060.0
64	1767.0
65	1474.0
66	1185.5
67	897.0
68	752.5
69	608.0
70	477.5
71	347.0
72	301.0
73	255.0
74	173.0
75	83.5
76	76.0
77	56.5
78	37.0
79	26.5
80	16.0
81	16.5
82	17.0
83	10.0
84	3.0
85	3.0
86	3.0
87	3.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	199601.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.307383229542936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.38310072487288	33.981793678388385
2	10.777885967759387	9.981913918266942
3	4.682462403981392	6.504977429972795
4	2.6766201449745752	4.957890992530097
5	1.9506653683868873	4.516510438324457
6	1.4627285513361463	4.064107895251025
7	1.1240939089040356	3.6437693197929866
8	0.9109596451368603	3.3747325915200825
9	0.6469760900140646	2.6963792766569306
>10	2.2687439143135344	15.158240690176902
>50	0.06924158822893	2.1547988236531883
>100	0.03894839337877313	3.7990791629300453
>500	0.0032456994482310936	1.293079693989509
>1k	0.004327599264308125	3.8727260885466506
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3853	1.9303510503454393	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCC	1383	0.6928823001888769	No Hit
CCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC	1318	0.6603173330794936	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC	1176	0.589175404932841	TruSeq Adapter, Index 14 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	997	0.49949649550853953	No Hit
CTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGCT	926	0.4639255314352132	TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC	658	0.3296576670457563	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCG	449	0.22494877280173947	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	444	0.2224437753317869	No Hit
ACTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC	426	0.21342578443995772	TruSeq Adapter, Index 21 (95% over 22bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	399	0.19989879810221392	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	398	0.19939779860822343	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	360	0.180359817836584	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGC	316	0.1583158401010015	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	297	0.14879684971518178	No Hit
ACCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG	286	0.14328585528128615	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	265	0.13276486590748543	No Hit
AGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG	260	0.13025986843753287	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTC	247	0.12374687501565623	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGT	241	0.12074087805171317	No Hit
CGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG	227	0.11372688513584601	TruSeq Adapter, Index 21 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG	223	0.11172288715988396	No Hit
GGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG	208	0.1042078947500263	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.49097950411070085	0.0
2	0.0	0.0	0.0	2.3942765817806526	0.0
3	0.0	0.0	0.0	3.3346526320008416	0.0
4	0.0	0.0	0.0	4.476931478299207	0.0
5	0.0	0.0	0.0	7.444351481205004	0.0
6	0.0	0.0	0.0	9.119192789615283	0.0
7	0.0	0.0	0.0	10.821088070701048	0.0
8	0.0	0.0	0.0	13.206346661589873	0.0
9	0.0	0.0	0.0	14.170269688027615	0.0
10	0.0	0.0	0.0	16.412743423129143	0.0
11	0.0	0.0	0.0	20.969834820466833	0.0
12	0.0	0.0	0.0	23.805491956453125	0.0
13	0.0	0.0	0.0	24.984343765812795	0.0
14	0.0	0.0	0.0	25.432237313440314	0.0
15	0.0	0.0	0.0	26.358585377828767	0.0
16	0.0	0.0	0.0	28.461781253600932	0.0
17	0.0	0.0	0.0	30.93872275189002	0.0
18	0.0	0.0	0.0	33.36756829875602	0.0
19	0.0	0.0	0.0	34.92868272203045	0.0
20	0.0	0.0	0.0	36.37857525763899	0.0
21	0.0	0.0	0.0	38.11403750482212	0.0
22	0.0	0.0	0.0	39.78286681930451	0.0
23	0.0	0.0	0.0	41.28035430684215	0.0
24	0.0	0.0	0.0	42.24978832771379	0.0
25	0.0	0.0	0.0	42.97623759400003	0.0
26	0.0	0.0	0.0	43.612506951367976	0.0
27	5.009994939905111E-4	0.0	0.0	44.13454842410609	0.0
28	5.009994939905111E-4	0.0	0.0	44.65107890241031	0.0
29	5.009994939905111E-4	0.0	0.0	45.188150359968134	0.0
30	5.009994939905111E-4	0.0	0.0	45.722716820056014	0.0
31	5.009994939905111E-4	0.0	0.0	46.18664235149122	0.0
32	5.009994939905111E-4	0.0	0.0	46.60096893302138	0.0
33	5.009994939905111E-4	0.0	0.0	46.98723954288806	0.0
34	5.009994939905111E-4	0.0	0.0	47.33693718969344	0.0
35	5.009994939905111E-4	0.0	0.0	47.726213796524064	0.0
36	5.009994939905111E-4	0.0	0.0	48.01579150405058	0.0
37	5.009994939905111E-4	0.0	0.0	48.341942174638405	0.0
38	5.009994939905111E-4	0.0	0.0	48.63202088165891	0.0
39	5.009994939905111E-4	0.0	0.0	48.949153561354905	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	20	7.019268E-4	45.000004	2
CGAAAGG	20	7.019268E-4	45.000004	2
TACGGGA	85	0.0	45.000004	4
CTGTTAA	20	7.019268E-4	45.000004	36
GCTAACG	20	7.019268E-4	45.000004	1
TGCGTGG	20	7.019268E-4	45.000004	2
GACTAGG	20	7.019268E-4	45.000004	2
ATGGGCA	90	0.0	45.0	5
GTACAGG	35	1.2051896E-7	45.0	2
TCGATAG	35	1.2051896E-7	45.0	1
TCAGGGT	25	3.8791914E-5	45.0	4
CGTTATT	90	0.0	45.0	1
CGTTAGG	25	3.8791914E-5	45.0	2
CTAACGG	25	3.8791914E-5	45.0	2
GGGACAC	35	1.2051896E-7	45.0	7
CGGGATG	35	1.2051896E-7	45.0	6
GACGGGA	45	3.8198777E-10	45.0	4
AGGGCCC	35	1.2051896E-7	45.0	6
GCGTAAG	25	3.8791914E-5	45.0	1
GTACGGG	45	3.8198777E-10	45.0	3
>>END_MODULE