FastQCFastQC Report
Sat 14 Jan 2017
SRR2934814.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934814.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312858
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47821.5284889630439369No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCC16510.5277154491814178No Hit
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC15740.5031036444649011TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC15720.5024643768099265TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT10760.34392599837626014TruSeq Adapter, Index 15 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7830.2502732869225016No Hit
TCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC6860.21926880565624024TruSeq Adapter, Index 21 (95% over 22bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.17963421104782362No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.177396774255413No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCG5490.17547897129048962No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCG5410.17292190067059177No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGC4850.15502240633130684No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTC4650.14862972978156225No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4580.14639229298915163No Hit
ACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC4520.14447449002422824TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGC4030.12881243247735397No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.11059330431058179No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAC302.1604192E-645.00000416
CCGGGAT207.025407E-445.0000045
ACACGAC207.025407E-445.00000426
GGTAATA207.025407E-445.0000048
CGCGGGC207.025407E-445.0000044
GACACGA207.025407E-445.00000425
GCGAAAG207.025407E-445.0000041
TTTCGCA406.7884685E-945.00000414
CACGACC207.025407E-445.00000427
CCGTCAG302.1604192E-645.00000430
ACTACGG207.025407E-445.0000042
CGGGACA406.7884685E-945.0000046
ACGGGTC351.2081364E-745.05
AATGCGG253.884277E-545.02
TAGGGCG253.884277E-545.05
CTCTATT253.884277E-545.033
CGTTATT1600.043.5937541
GACTAAT850.042.352949
AACTGGG650.041.5384643
CTAGATA603.6379788E-1241.25000419
Produced by FastQC (version 0.11.5)