SRR2934813.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934813.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	170294
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3580	2.102246702761107	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCC	1187	0.6970298425076632	No Hit
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	1118	0.656511679800815	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1092	0.6412439663170751	No Hit
CCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	820	0.481520194487181	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	794	0.46625248100344113	No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	650	0.38169283709349716	Illumina Single End Adapter 2 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	560	0.3288430596497821	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	473	0.277754941454191	No Hit
TCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	440	0.2583766897248288	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	372	0.2184457467673553	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	316	0.18556144080237705	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCG	305	0.17910202355925633	No Hit
ACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	302	0.1773403643111325	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	272	0.1597237718298942	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGC	234	0.13740942135365897	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	230	0.13506054235616052	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	216	0.12683946586491596	No Hit
GCCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTG	207	0.12155448812054447	No Hit
AAGGAGGGAACACTAGTATATTTATATTTTAATCTATTTTAGTTTTTACTT	193	0.11333341162929993	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTCG	25	3.8767706E-5	45.000004	10
GTCATCG	25	3.8767706E-5	45.000004	34
GAGTACG	25	3.8767706E-5	45.000004	1
TCCTCTA	25	3.8767706E-5	45.000004	21
TACGGGC	25	3.8767706E-5	45.000004	4
ACGGGAC	25	3.8767706E-5	45.000004	5
CCCACGG	25	3.8767706E-5	45.000004	28
GTAGACC	25	3.8767706E-5	45.000004	16
CGTAGAC	25	3.8767706E-5	45.000004	15
TGTTCGT	25	3.8767706E-5	45.000004	11
TCGTAGA	25	3.8767706E-5	45.000004	14
TCATCTC	25	3.8767706E-5	45.000004	16
TCATCGA	25	3.8767706E-5	45.000004	35
CATAAGG	25	3.8767706E-5	45.000004	2
CATCGAT	25	3.8767706E-5	45.000004	36
GTATAAG	25	3.8767706E-5	45.000004	1
TAAGGTA	25	3.8767706E-5	45.000004	4
TGCTAAG	25	3.8767706E-5	45.000004	1
TCATACA	25	3.8767706E-5	45.000004	8
AGTACGG	50	2.1827873E-11	45.000004	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content