##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934813.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	170294
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74889309077243	31.0	31.0	34.0	30.0	34.0
2	31.84266621255006	33.0	31.0	34.0	30.0	34.0
3	31.841550495026247	33.0	31.0	34.0	30.0	34.0
4	35.63141977991004	37.0	35.0	37.0	33.0	37.0
5	33.016718146264694	37.0	35.0	37.0	28.0	37.0
6	34.068645988701896	37.0	35.0	37.0	27.0	37.0
7	35.39053049432159	37.0	35.0	37.0	32.0	37.0
8	35.30950004110538	37.0	35.0	37.0	33.0	37.0
9	37.37529801402281	39.0	37.0	39.0	34.0	39.0
10	36.9199971813452	39.0	37.0	39.0	33.0	39.0
11	36.7475131243614	39.0	37.0	39.0	32.0	39.0
12	37.01925493558199	39.0	37.0	39.0	33.0	39.0
13	36.92521756491714	39.0	37.0	39.0	33.0	39.0
14	37.872473487028316	40.0	37.0	41.0	33.0	41.0
15	38.06155237412945	40.0	37.0	41.0	33.0	41.0
16	38.0659154168673	40.0	37.0	41.0	33.0	41.0
17	38.049185526207616	40.0	37.0	41.0	33.0	41.0
18	37.93028527135424	39.0	37.0	41.0	34.0	41.0
19	37.72616768647163	39.0	36.0	41.0	34.0	41.0
20	37.51376443092534	39.0	35.0	40.0	33.0	41.0
21	37.40615641185244	39.0	35.0	40.0	33.0	41.0
22	37.36105206290298	39.0	35.0	40.0	33.0	41.0
23	37.1419662466088	39.0	35.0	40.0	33.0	41.0
24	36.866806816446854	39.0	35.0	40.0	32.0	41.0
25	36.66502636616675	38.0	35.0	40.0	32.0	41.0
26	36.918276627479536	39.0	35.0	40.0	32.0	41.0
27	36.80476117772793	39.0	35.0	40.0	32.0	41.0
28	36.90733085135119	39.0	35.0	40.0	32.0	41.0
29	36.93086074670863	39.0	35.0	40.0	33.0	41.0
30	36.58238693083726	38.0	35.0	40.0	31.0	41.0
31	36.48342865867265	39.0	35.0	40.0	31.0	41.0
32	36.237225034352356	39.0	35.0	40.0	30.0	41.0
33	35.98398651743455	39.0	35.0	40.0	30.0	41.0
34	35.78190658508227	39.0	35.0	40.0	29.0	41.0
35	35.52313645812536	39.0	35.0	40.0	25.0	41.0
36	35.267073414213066	38.0	35.0	40.0	24.0	41.0
37	35.15480287033014	38.0	35.0	40.0	24.0	41.0
38	35.10955171644333	38.0	35.0	40.0	23.0	41.0
39	35.01438688385968	38.0	35.0	40.0	23.0	41.0
40	34.86096397994057	38.0	35.0	40.0	22.0	41.0
41	34.81349313540113	38.0	35.0	40.0	22.0	41.0
42	34.81138501650088	38.0	35.0	40.0	21.0	41.0
43	34.62955829330452	38.0	35.0	40.0	21.0	41.0
44	34.549714023982055	38.0	34.0	40.0	20.0	41.0
45	34.599604213888924	38.0	34.0	40.0	20.0	41.0
46	34.51406391299752	38.0	34.0	40.0	20.0	41.0
47	34.45800204352473	38.0	34.0	40.0	20.0	41.0
48	34.4035726449552	38.0	34.0	40.0	20.0	41.0
49	34.3829142541722	38.0	34.0	40.0	20.0	41.0
50	34.268424019636626	38.0	34.0	40.0	20.0	41.0
51	33.394964003429365	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	4.0
11	5.0
12	4.0
13	2.0
14	9.0
15	12.0
16	26.0
17	35.0
18	78.0
19	149.0
20	263.0
21	422.0
22	613.0
23	948.0
24	1426.0
25	2182.0
26	3030.0
27	3594.0
28	3452.0
29	3431.0
30	3583.0
31	4052.0
32	4955.0
33	6689.0
34	10670.0
35	14915.0
36	17226.0
37	23065.0
38	35499.0
39	29941.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.23384264859595	22.031897776786032	22.96557717829166	17.768682396326355
2	37.2767096903003	24.65442117749304	23.26975700846771	14.799112123738944
3	27.860641009078417	23.27739086520958	35.35062891235158	13.511339213360424
4	24.78184786310733	25.53818690030183	34.64831409209955	15.031651144491292
5	19.28136047071535	33.45156024287409	31.77504785840957	15.492031428000985
6	20.3395304590884	34.272493452499795	33.33822683124479	12.049749257167017
7	64.16139147591812	8.551093990393085	21.71362467262499	5.573889861063807
8	61.14014586538574	9.074894006835239	22.441777161849508	7.343182965929509
9	55.767672378357425	10.654515132652943	25.363195414988198	8.214617074001433
10	29.498984109833582	27.204129329277606	30.600021139910975	12.696865420977838
11	22.427683887864518	23.536941994433157	35.04644908217553	18.988925035526794
12	20.797561863600595	22.284989488766485	40.115329958777174	16.802118688855742
13	20.004815201944872	24.51055233889626	40.30793803657205	15.17669442258682
14	14.66463880113216	28.326306270332484	38.73301466874934	18.276040259786015
15	13.427366789199855	25.826511797244766	43.6721199807392	17.07400143281619
16	13.49313540112981	26.711451959552303	39.9597167251929	19.835695914124983
17	14.21659013235933	25.728446099099205	37.03183905481109	23.023124713730372
18	13.721563883636534	26.448964731581853	41.557541663241224	18.271929721540396
19	16.331168449857305	26.248135577295734	38.89508731957673	18.525608653270226
20	16.586609040835263	28.34627174181122	38.30493147145525	16.76218774589827
21	15.82615946539514	27.075528204164563	39.72717770444056	17.371134625999744
22	12.646364522531622	27.988654914442083	36.22323745992225	23.141743103104044
23	14.153757619176249	25.790691392532917	37.84690006694305	22.208650921347786
24	15.632376948101518	25.64095035644239	37.75940432428623	20.967268371169858
25	13.61410266950098	27.59991544035609	36.50451571987269	22.28146617027024
26	12.079110244635748	29.771454073543403	39.00900795095541	19.140427730865444
27	15.523741294467216	29.073249791536988	37.12520699496166	18.277801919034143
28	11.542978613456729	27.258740765969442	38.53747049220759	22.660810128366236
29	14.168438112910614	24.909861768470996	39.70721223296182	21.21448788565657
30	15.903085252563216	31.161990440062482	34.00002348878998	18.934900818584328
31	16.52671262639905	30.644649840863448	31.51373506993787	21.31490246279963
32	17.192619822189858	29.861885914947088	33.05988467004122	19.885609592821826
33	18.913760907606843	30.050970674245715	30.278811937003063	20.756456481144372
34	17.295383278330416	26.870588511632825	33.349971226232284	22.48405698380448
35	19.049995889461755	26.625130656394237	33.431007551645976	20.89386590249803
36	20.50395198891329	31.063337522167544	30.102058792441305	18.330651696477858
37	20.318390548110916	28.241159406673166	31.957085980715703	19.483364064500215
38	18.632482647656406	30.019848027528862	30.26119534452183	21.086473980292904
39	23.073038392427215	26.480674598048083	31.696360411993375	18.749926597531328
40	24.563402116339976	25.750760449575438	32.100954819312484	17.5848826147721
41	24.02139828766721	26.423714282358745	28.75908722562157	20.79580020435247
42	23.313798489670802	25.702608430126723	31.290591565175518	19.693001515026953
43	22.591518197940033	24.628583508520556	32.52199137961408	20.257906913925332
44	18.75932211352132	25.357910437243824	31.675807720765263	24.206959728469588
45	21.28847757407777	24.39839336676571	31.33052250813299	22.982606551023522
46	22.86222650240173	26.823610931682857	31.864305260314513	18.449857305600904
47	20.752933162648127	25.34146828426134	34.607208709643324	19.298389843447215
48	20.633140333775707	23.761259938694256	33.61656899244835	21.989030735081684
49	22.923884576086063	21.586785206760073	35.50330604718898	19.98602416996488
50	20.377112523048375	21.436456950920174	34.17618941360236	24.010241112429092
51	20.664850200241936	20.546231810868264	30.59473616216661	28.194181826723195
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1213.0
1	2034.0
2	2855.0
3	4750.0
4	6645.0
5	4420.0
6	2195.0
7	2226.5
8	2258.0
9	2189.0
10	2120.0
11	2212.5
12	2305.0
13	2306.0
14	2307.0
15	2070.5
16	1834.0
17	1743.5
18	1653.0
19	1710.5
20	1768.0
21	1948.0
22	2128.0
23	1890.5
24	1653.0
25	2265.5
26	2796.5
27	2715.0
28	2573.5
29	2432.0
30	2533.0
31	2634.0
32	2872.0
33	3110.0
34	3122.0
35	3134.0
36	3352.5
37	3571.0
38	3941.5
39	4312.0
40	4938.0
41	5564.0
42	7133.0
43	8702.0
44	9986.0
45	11270.0
46	13611.5
47	15953.0
48	18174.5
49	20396.0
50	19732.5
51	19069.0
52	15010.5
53	10952.0
54	8473.0
55	5994.0
56	4844.5
57	3695.0
58	3336.0
59	2977.0
60	3017.5
61	3058.0
62	2612.0
63	2166.0
64	1960.5
65	1755.0
66	1462.0
67	1169.0
68	970.5
69	772.0
70	601.5
71	431.0
72	356.0
73	281.0
74	228.5
75	141.5
76	107.0
77	67.0
78	27.0
79	31.5
80	36.0
81	26.5
82	17.0
83	9.0
84	1.0
85	2.0
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	170294.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.82545480169589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.13439606345626	33.97242416056937
2	10.558958456136754	9.677381469693588
3	4.469617365898665	6.144667457455929
4	2.5910453881442375	4.749433332941853
5	1.8311591788615804	4.195685109281595
6	1.3198698070170942	3.629018051135096
7	1.0597401266049873	3.399415129129623
8	0.8406161101002075	3.0817292447179585
9	0.6637791844998591	2.7376184715844363
>10	2.394987057587329	16.416315313516623
>50	0.08841846280017428	2.7769621947925356
>100	0.03716138291601527	3.4640093015608304
>500	0.0051257079884159005	1.6583085722339013
>1k	0.0051257079884159005	4.097032191386661
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3580	2.102246702761107	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCC	1187	0.6970298425076632	No Hit
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	1118	0.656511679800815	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1092	0.6412439663170751	No Hit
CCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	820	0.481520194487181	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	794	0.46625248100344113	No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	650	0.38169283709349716	Illumina Single End Adapter 2 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	560	0.3288430596497821	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	473	0.277754941454191	No Hit
TCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	440	0.2583766897248288	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	372	0.2184457467673553	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	316	0.18556144080237705	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCG	305	0.17910202355925633	No Hit
ACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	302	0.1773403643111325	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	272	0.1597237718298942	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGC	234	0.13740942135365897	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	230	0.13506054235616052	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	216	0.12683946586491596	No Hit
GCCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTG	207	0.12155448812054447	No Hit
AAGGAGGGAACACTAGTATATTTATATTTTAATCTATTTTAGTTTTTACTT	193	0.11333341162929993	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4010710888228593	0.0
2	0.0	0.0	0.0	2.074060154791126	0.0
3	0.0	0.0	0.0	2.798689325519396	0.0
4	0.0	0.0	0.0	3.720624332037535	0.0
5	0.0	0.0	0.0	6.219831585375879	0.0
6	0.0	0.0	0.0	7.619763467884952	0.0
7	0.0	0.0	0.0	9.189989077712662	0.0
8	0.0	0.0	0.0	11.511855966739873	0.0
9	0.0	0.0	0.0	12.408540524034905	0.0
10	0.0	0.0	0.0	14.826124232210178	0.0
11	0.0	0.0	0.0	18.863260009160626	0.0
12	0.0	0.0	0.0	21.563883636534463	0.0
13	0.0	0.0	0.0	22.626164163153135	0.0
14	0.0	0.0	0.0	23.069515073930965	0.0
15	0.0	0.0	0.0	23.9638507522285	0.0
16	0.0	0.0	0.0	25.858221663710992	0.0
17	0.0	0.0	0.0	28.103162765570133	0.0
18	0.0	0.0	0.0	30.55715409820663	0.0
19	0.0	0.0	0.0	31.939469388234464	0.0
20	0.0	0.0	0.0	33.19083467415176	0.0
21	0.0	0.0	0.0	34.769281360470714	0.0
22	0.0	0.0	0.0	36.276087237365964	0.0
23	0.0	0.0	0.0	37.61847158443633	0.0
24	0.0	0.0	0.0	38.56448260067883	0.0
25	0.0	0.0	0.0	39.298507287397086	0.0
26	0.0	0.0	0.0	39.92330910073168	0.0
27	0.0	0.0	0.0	40.445347457925706	0.0
28	0.0	0.0	0.0	40.932152630157255	0.0
29	0.0	0.0	0.0	41.43598717512067	0.0
30	0.0	0.0	0.0	41.89460579938225	0.0
31	0.0	0.0	0.0	42.31975289792946	0.0
32	0.0	0.0	0.0	42.709079591764834	0.0
33	0.0	0.0	0.0	43.05612646364523	0.0
34	5.87219749374611E-4	0.0	0.0	43.38790562204188	0.0
35	5.87219749374611E-4	0.0	0.0	43.76724958013788	0.0
36	5.87219749374611E-4	0.0	0.0	44.102552057030785	0.0
37	5.87219749374611E-4	0.0	0.0	44.409080766204326	0.0
38	5.87219749374611E-4	0.0	0.0	44.69975454214476	0.0
39	5.87219749374611E-4	0.0	0.0	44.92877024440086	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTCG	25	3.8767706E-5	45.000004	10
GTCATCG	25	3.8767706E-5	45.000004	34
GAGTACG	25	3.8767706E-5	45.000004	1
TCCTCTA	25	3.8767706E-5	45.000004	21
TACGGGC	25	3.8767706E-5	45.000004	4
ACGGGAC	25	3.8767706E-5	45.000004	5
CCCACGG	25	3.8767706E-5	45.000004	28
GTAGACC	25	3.8767706E-5	45.000004	16
CGTAGAC	25	3.8767706E-5	45.000004	15
TGTTCGT	25	3.8767706E-5	45.000004	11
TCGTAGA	25	3.8767706E-5	45.000004	14
TCATCTC	25	3.8767706E-5	45.000004	16
TCATCGA	25	3.8767706E-5	45.000004	35
CATAAGG	25	3.8767706E-5	45.000004	2
CATCGAT	25	3.8767706E-5	45.000004	36
GTATAAG	25	3.8767706E-5	45.000004	1
TAAGGTA	25	3.8767706E-5	45.000004	4
TGCTAAG	25	3.8767706E-5	45.000004	1
TCATACA	25	3.8767706E-5	45.000004	8
AGTACGG	50	2.1827873E-11	45.000004	2
>>END_MODULE