##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934811.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	228168
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.780183899582763	31.0	31.0	34.0	30.0	34.0
2	31.891842852634902	33.0	31.0	34.0	30.0	34.0
3	31.900362890501736	33.0	31.0	34.0	30.0	34.0
4	35.65854107499737	37.0	35.0	37.0	33.0	37.0
5	33.04774113810876	37.0	35.0	37.0	28.0	37.0
6	34.129645699659896	37.0	35.0	37.0	28.0	37.0
7	35.48174590652501	37.0	35.0	37.0	32.0	37.0
8	35.48560271378984	37.0	35.0	37.0	33.0	37.0
9	37.572722730619546	39.0	37.0	39.0	35.0	39.0
10	36.93032327057256	39.0	37.0	39.0	32.0	39.0
11	36.70894691630728	39.0	37.0	39.0	32.0	39.0
12	37.05624364503348	39.0	37.0	39.0	33.0	39.0
13	37.07152186108481	39.0	37.0	39.0	33.0	39.0
14	38.11024771221206	40.0	37.0	41.0	33.0	41.0
15	38.272623680796606	40.0	38.0	41.0	34.0	41.0
16	38.22263419936187	40.0	37.0	41.0	33.0	41.0
17	38.192336348655374	40.0	37.0	41.0	34.0	41.0
18	38.06922969040356	39.0	37.0	41.0	34.0	41.0
19	37.87569247221346	39.0	37.0	41.0	34.0	41.0
20	37.69277023947267	39.0	35.0	41.0	34.0	41.0
21	37.608674310157426	39.0	35.0	41.0	33.0	41.0
22	37.56016619333123	39.0	35.0	41.0	33.0	41.0
23	37.34755969285789	39.0	35.0	40.0	33.0	41.0
24	37.11250043827355	39.0	35.0	40.0	32.0	41.0
25	36.91383541951544	39.0	35.0	40.0	32.0	41.0
26	37.15314154482662	39.0	35.0	40.0	33.0	41.0
27	36.98531345324498	39.0	35.0	40.0	33.0	41.0
28	37.0380202307072	39.0	35.0	40.0	33.0	41.0
29	37.099759826093056	39.0	35.0	41.0	33.0	41.0
30	36.824212860699134	39.0	35.0	40.0	32.0	41.0
31	36.61778601732057	39.0	35.0	40.0	31.0	41.0
32	36.28324743171698	39.0	35.0	40.0	30.0	41.0
33	36.06528522842817	39.0	35.0	40.0	30.0	41.0
34	35.93921145822377	39.0	35.0	41.0	29.0	41.0
35	35.69128449212861	39.0	35.0	41.0	26.0	41.0
36	35.549476701377934	39.0	35.0	40.0	25.0	41.0
37	35.43871182637355	39.0	35.0	40.0	24.0	41.0
38	35.30068195364819	39.0	35.0	40.0	24.0	41.0
39	35.22071017846499	39.0	35.0	40.0	23.0	41.0
40	35.16800340100277	39.0	35.0	40.0	23.0	41.0
41	35.098217103187125	39.0	35.0	40.0	23.0	41.0
42	35.15744977385085	39.0	35.0	40.0	23.0	41.0
43	35.01853458854879	39.0	35.0	40.0	22.0	41.0
44	34.906424213737246	39.0	35.0	40.0	22.0	41.0
45	34.88431769573297	38.0	35.0	40.0	22.0	41.0
46	34.7525332211353	38.0	34.0	40.0	22.0	41.0
47	34.65157690824305	38.0	34.0	40.0	21.0	41.0
48	34.62751130745766	38.0	34.0	40.0	20.0	41.0
49	34.60173643981628	38.0	34.0	40.0	20.0	41.0
50	34.512578450965954	38.0	34.0	40.0	20.0	41.0
51	33.53774411836892	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	5.0
11	5.0
12	3.0
13	8.0
14	10.0
15	16.0
16	35.0
17	58.0
18	118.0
19	189.0
20	347.0
21	493.0
22	899.0
23	1267.0
24	1838.0
25	2933.0
26	3917.0
27	4433.0
28	4310.0
29	4156.0
30	4422.0
31	4930.0
32	6265.0
33	8487.0
34	13210.0
35	18595.0
36	23174.0
37	30712.0
38	47016.0
39	46299.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.07839837312857	22.633761088320885	25.431699449528416	17.856141089022124
2	37.12308474457417	23.147855965779602	24.461800077136147	15.267259212510082
3	27.307510255601137	22.798990217734303	35.900301532204345	13.993197994460221
4	23.795186003295818	24.858437642438904	35.87356684548227	15.472809508783003
5	18.673082991479962	33.014708460432665	32.49623084744574	15.815977700641632
6	21.833911854423057	32.29550156025385	33.49505627432418	12.375530310998913
7	69.48388906419831	6.467164545422671	19.701711019950213	4.347235370428807
8	68.67965709477227	5.4617650152519195	20.068545983661163	5.7900319063146455
9	62.873847340556075	8.533624346972406	22.469408506013114	6.1231198064584
10	29.254759650783633	30.43283896076575	28.68894849409207	11.623452894358541
11	18.914133445531363	23.345517338101747	39.87894884471091	17.86140037165597
12	18.41493986886855	21.424126082535675	43.34437782686442	16.816556221731354
13	18.75723151362154	23.360856912450476	43.405297850706496	14.476613723221485
14	15.529346797096878	26.430086602854036	40.81553942708881	17.225027172960274
15	13.851635636899125	25.841485221415798	43.68710774517022	16.61977139651485
16	14.38282318291785	26.788594369061393	40.73796500823954	18.090617439781216
17	14.841695592721152	25.255075207741662	38.64038778443953	21.262841415097647
18	14.45557659268609	25.711756249780866	42.16191578135409	17.670751376178956
19	15.683619087689774	26.403351916131975	39.7264296483293	18.186599347848954
20	16.008379790329933	28.70297324778234	39.193050734546475	16.095596227341257
21	15.750236667718523	27.74928999684443	40.066968198871	16.43350513656604
22	13.892395077311454	26.56595140422846	37.94572420321868	21.595929315241403
23	13.38969531222608	26.241628975141122	39.1132849479331	21.255390764699694
24	15.428543879948108	25.321254514217596	38.83191332702219	20.418288278812103
25	13.958574383787385	27.83037060411627	36.871953998807896	21.339101013288452
26	14.842572139826792	28.829634304547525	37.637617895585706	18.69017566003997
27	17.01377932050068	27.330300480347812	38.217892079520354	17.43802811963115
28	13.724974580133937	26.2644191998878	39.72555310122366	20.2850531187546
29	16.445338522492197	25.69729322253778	37.74236527470986	20.11500298026016
30	19.66445776796045	27.37544265628835	34.89358718137513	18.066512394376076
31	18.054679008449913	30.325900213877492	33.28994425160408	18.32947652606851
32	17.93765996984678	31.923407313909046	31.155113775814314	18.98381894042986
33	18.38995827635777	30.053294064023	31.222607902948706	20.334139756670524
34	18.877756740647243	27.12781809894464	33.154517723782476	20.839907436625644
35	19.829686897373865	29.291136355667753	29.958626976613722	20.92054977034466
36	22.122295852179096	28.11831632831948	30.819396234353636	18.939991585147787
37	19.621068686231197	28.744170961747486	34.34837488166614	17.286385470355174
38	16.618456575856385	32.835892850881805	30.65986466112689	19.885785912134917
39	19.712229585217912	30.386820237719576	29.525174432874024	20.37577574418849
40	22.490007362995687	28.59165176536587	30.620420041373027	18.29792083026542
41	21.09454437081449	29.434451807440134	27.526647032011496	21.94435678973388
42	21.506083236913152	26.273184670944218	31.0302058132604	21.190526278882228
43	21.001192104063673	26.853020581326042	31.794116615826933	20.35167069878335
44	19.399740542056733	27.320658462185758	30.862785316082885	22.416815679674627
45	21.711633533186074	26.59400091160899	30.027435924406575	21.66692963079836
46	23.372690298376636	27.095824129588724	31.21997826163178	18.31150731040286
47	18.608218505662492	28.166088145576946	33.567809684092424	19.657883664668137
48	18.08272851583044	26.73775463693419	32.70747869990534	22.472038147330036
49	19.98395918796676	23.8688159601697	34.30410925283125	21.84311559903229
50	18.976806563584727	23.882402440307143	33.33508642754461	23.805704568563517
51	18.243136636162827	23.9998597524631	30.188720591844604	27.56828301952947
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1316.0
1	2039.5
2	2763.0
3	5495.5
4	8228.0
5	5485.0
6	2742.0
7	2813.5
8	2885.0
9	3076.5
10	3268.0
11	3183.0
12	3098.0
13	3061.5
14	3025.0
15	3260.0
16	3495.0
17	3540.5
18	3586.0
19	3281.0
20	2976.0
21	2691.0
22	2406.0
23	2380.0
24	2354.0
25	2740.0
26	3104.5
27	3083.0
28	3247.0
29	3411.0
30	3700.5
31	3990.0
32	4391.5
33	4793.0
34	4952.5
35	5112.0
36	5755.0
37	6398.0
38	6619.0
39	6840.0
40	7837.5
41	8835.0
42	10642.0
43	12449.0
44	13756.0
45	15063.0
46	17574.5
47	20086.0
48	23215.0
49	26344.0
50	25383.5
51	24423.0
52	19479.0
53	14535.0
54	11616.0
55	8697.0
56	6913.0
57	5129.0
58	4618.5
59	4108.0
60	3766.5
61	3425.0
62	2933.0
63	2441.0
64	2066.5
65	1692.0
66	1268.0
67	844.0
68	675.0
69	506.0
70	415.5
71	325.0
72	251.5
73	178.0
74	140.0
75	81.5
76	61.0
77	36.5
78	12.0
79	7.5
80	3.0
81	5.5
82	8.0
83	7.0
84	6.0
85	3.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	228168.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.07710616646316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.94659304418599	32.88221319134555
2	10.834407922024306	9.767675123037185
3	4.562075744284536	6.169355179936814
4	2.5966905689327113	4.682051858289338
5	1.8610229980276807	4.194476563016166
6	1.4732244901075153	3.9845218048566013
7	1.1513937903838702	3.6331050089978105
8	0.9185163943371205	3.3123248818537068
9	0.7416095367626172	3.0086650640439054
>10	2.8095207651889353	18.509282243482044
>50	0.06896369007397181	2.0949824119991343
>100	0.028984739306451922	2.88702092389042
>500	0.0029984213075639923	1.1439054503367316
>1k	0.00399789507675199	3.7304202949145875
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4841	2.1216822692051474	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCC	1338	0.5864100136741349	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	1088	0.47684162546895276	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	1013	0.443971109007398	TruSeq Adapter, Index 14 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	901	0.39488447109147645	No Hit
TAAACAGGGAGATGGATATAAATATTTTTTTTATTTTTAAATATTGTACTA	834	0.36552014305248764	No Hit
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT	804	0.3523719364678658	TruSeq Adapter, Index 15 (95% over 23bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	472	0.2068651169313839	No Hit
TCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	452	0.1980996458749693	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG	439	0.19240208968829983	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	403	0.17662424178675362	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	342	0.14988955506468918	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	325	0.1424389046667368	No Hit
ACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	313	0.13717962203288805	TruSeq Adapter, Index 15 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	312	0.1367413484800673	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	300	0.13148206584621858	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.38524245292942044	0.0
2	0.0	0.0	0.0	1.781143718663441	0.0
3	0.0	0.0	0.0	2.403053890116055	0.0
4	0.0	0.0	0.0	3.229637810735949	0.0
5	0.0	0.0	0.0	5.335980505592371	0.0
6	0.0	0.0	0.0	6.5096770800462815	0.0
7	0.0	0.0	0.0	7.727201009782266	0.0
8	0.0	0.0	0.0	9.669629395883735	0.0
9	0.0	0.0	0.0	10.443620490165141	0.0
10	0.0	0.0	0.0	12.377283405210196	0.0
11	0.0	0.0	0.0	15.954472143332982	0.0
12	0.0	0.0	0.0	18.38294589951264	0.0
13	0.0	0.0	0.0	19.266067108446407	0.0
14	0.0	0.0	0.0	19.61624767715017	0.0
15	0.0	0.0	0.0	20.30170751376179	0.0
16	0.0	0.0	0.0	21.906226990638476	0.0
17	0.0	0.0	0.0	23.967865783107182	0.0
18	0.0	0.0	0.0	26.039146593737946	0.0
19	0.0	0.0	0.0	27.27463973913958	0.0
20	0.0	0.0	0.0	28.44658321938221	0.0
21	0.0	0.0	0.0	29.82451526945058	0.0
22	0.0	0.0	0.0	31.216033799656394	0.0
23	0.0	0.0	0.0	32.454594859927774	0.0
24	0.0	0.0	0.0	33.328074050699485	0.0
25	0.0	0.0	0.0	34.023614179025984	0.0
26	0.0	0.0	0.0	34.58811051505908	0.0
27	0.0	0.0	0.0	35.110532590021386	0.0
28	0.0	0.0	0.0	35.66319554012833	0.0
29	0.0	0.0	0.0	36.20709301917885	0.0
30	0.0	0.0	0.0	36.779478279162724	0.0
31	0.0	0.0	0.0	37.27911012937835	0.0
32	0.0	0.0	0.0	37.749377651554994	0.0
33	0.0	0.0	0.0	38.15916342344238	0.0
34	0.0	0.0	0.0	38.575523298622066	0.0
35	0.0	0.0	0.0	39.001086918411	0.0
36	0.0	0.0	0.0	39.36047123172399	0.0
37	0.0	0.0	0.0	39.71722590372006	0.0
38	0.0	0.0	0.0	40.089320150064864	0.0
39	0.0	0.0	0.0	40.472371235230185	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATGG	25	3.8809478E-5	45.000004	2
GGCCGGT	25	3.8809478E-5	45.000004	9
TTGACGG	25	3.8809478E-5	45.000004	2
GCGGGCA	25	3.8809478E-5	45.000004	5
TTGCACG	25	3.8809478E-5	45.000004	1
TCGCATG	20	7.021391E-4	45.0	1
CCGGGAT	35	1.2062083E-7	45.0	5
GCGAATG	20	7.021391E-4	45.0	1
ACGTTAG	20	7.021391E-4	45.0	1
AGGACGG	20	7.021391E-4	45.0	2
TCGGGCA	20	7.021391E-4	45.0	5
GTAATGG	35	1.2062083E-7	45.0	2
CAGGGTA	75	0.0	45.0	5
ATCCGGG	20	7.021391E-4	45.0	3
GGGACGA	20	7.021391E-4	45.0	7
GCGATTG	20	7.021391E-4	45.0	9
CAGTGCG	20	7.021391E-4	45.0	1
TAGATTG	20	7.021391E-4	45.0	1
CGTAAGG	35	1.2062083E-7	45.0	2
GGGTTAC	20	7.021391E-4	45.0	8
>>END_MODULE