SRR2934809.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934809.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	249478
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3860	1.5472306175294013	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	2309	0.9255325118848156	No Hit
GCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	2118	0.8489726549034383	No Hit
CTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGCT	1655	0.6633851481894195	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCC	1474	0.5908336606835072	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1353	0.5423323900303835	No Hit
TCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	1249	0.5006453474855498	No Hit
ACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	704	0.2821892110727199	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	643	0.2577381572723847	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTG	520	0.20843521272416807	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCG	472	0.18919503924193717	No Hit
AGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTG	409	0.16394231154650912	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGC	392	0.15712808343821902	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTG	384	0.1539213878578472	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	379	0.1519172031201148	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	376	0.1507146922774754	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	281	0.11263518226056005	No Hit
AGCCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	275	0.11023016057528118	No Hit
GGCCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	262	0.10501928025717698	No Hit
GGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTG	259	0.10381676941453755	No Hit
TGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTG	257	0.1030150955194446	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	30	2.1586457E-6	45.000004	6
CCGGGAT	30	2.1586457E-6	45.000004	5
TGAACGG	35	1.2068267E-7	45.000004	2
GGATCAC	30	2.1586457E-6	45.000004	8
CGTTAGG	35	1.2068267E-7	45.000004	2
CTAACGG	30	2.1586457E-6	45.000004	2
AATCTGG	30	2.1586457E-6	45.000004	2
GTATAAG	35	1.2068267E-7	45.000004	1
TAGGCAG	30	2.1586457E-6	45.000004	1
GCTACCA	30	2.1586457E-6	45.000004	2
AGCTACC	30	2.1586457E-6	45.000004	1
TATACGG	30	2.1586457E-6	45.000004	2
GTGTTAG	45	3.8198777E-10	45.0	1
CGGGTAT	20	7.022659E-4	45.0	6
TCGCAGG	25	3.8819977E-5	45.0	2
CGAACGG	20	7.022659E-4	45.0	2
GATGTCG	20	7.022659E-4	45.0	9
CAAGTCG	20	7.022659E-4	45.0	1
TTGTGAC	20	7.022659E-4	45.0	18
TGGGTTA	25	3.8819977E-5	45.0	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content