FastQCFastQC Report
Sat 14 Jan 2017
SRR2934808.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934808.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200778
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30391.5136120491288885No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC20831.0374642640129894TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC16520.8227993106814493TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT11300.5628106665072866TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC10560.5259540387891104TruSeq Adapter, Index 16 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9630.47963422287302393No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8210.408909342657064No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCC8130.40492484236320714No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA7230.36009921405731704No Hit
ACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC6450.3212503361922123TruSeq Adapter, Index 13 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4560.22711651674984312No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4500.22412814152945043No Hit
ACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG3650.1817928259072209No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCG3290.1638625745848649No Hit
AGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG3180.15838388668081163No Hit
GCCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG2940.14643038579924095No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.14493619818904463No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.12650788432995647No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.11853888374224267No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.11505244598511788No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG2250.11206407076472522No Hit
GACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT2150.1070834453974041No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2070.1030989451035472No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATT207.0193666E-445.05
TCGCCGC207.0193666E-445.028
ATCCTAG253.8792725E-545.014
TGTCACG207.0193666E-445.01
ACTATGG253.8792725E-545.02
AGGTAAG207.0193666E-445.01
ACTATAT351.2052442E-745.015
ACCGGGA207.0193666E-445.04
ATAGGGT207.0193666E-445.04
CGCGGGA351.2052442E-745.04
ACGCAGT207.0193666E-445.02
GACAATA207.0193666E-445.09
CGCATGG207.0193666E-445.02
ATGTGAG207.0193666E-445.01
GGACTCC207.0193666E-445.08
TAGCGGG351.2052442E-745.03
TCGGGGT351.2052442E-745.05
TGAACGG253.8792725E-545.02
ATGCTAG253.8792725E-545.01
TAAGTAG207.0193666E-445.01
Produced by FastQC (version 0.11.5)