##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934808.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	200778
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.559777465658588	31.0	31.0	34.0	30.0	34.0
2	31.659997609299825	31.0	31.0	34.0	30.0	34.0
3	31.701306916096385	31.0	31.0	34.0	30.0	34.0
4	35.50700773989182	37.0	35.0	37.0	33.0	37.0
5	32.94718544860493	37.0	35.0	37.0	28.0	37.0
6	33.972671308609506	37.0	35.0	37.0	27.0	37.0
7	35.32400462202034	37.0	35.0	37.0	32.0	37.0
8	35.30325533674008	37.0	35.0	37.0	33.0	37.0
9	37.318660411001204	39.0	37.0	39.0	34.0	39.0
10	36.74391616611382	39.0	37.0	39.0	32.0	39.0
11	36.57755829821993	39.0	35.0	39.0	32.0	39.0
12	36.931073125541644	39.0	37.0	39.0	33.0	39.0
13	36.820005179850384	39.0	37.0	39.0	33.0	39.0
14	37.6877546344719	39.0	37.0	41.0	33.0	41.0
15	37.88378208767893	39.0	37.0	41.0	33.0	41.0
16	37.8904312225443	39.0	37.0	41.0	33.0	41.0
17	37.894072059687815	39.0	37.0	41.0	33.0	41.0
18	37.848912729482315	39.0	37.0	40.0	33.0	41.0
19	37.70601858769387	39.0	36.0	40.0	33.0	41.0
20	37.53421191564813	39.0	35.0	40.0	33.0	41.0
21	37.44593033101236	39.0	35.0	40.0	33.0	41.0
22	37.393135702118755	39.0	35.0	40.0	33.0	41.0
23	37.174979330404724	39.0	35.0	40.0	32.0	41.0
24	36.88007152178027	39.0	35.0	40.0	32.0	41.0
25	36.72369482712249	38.0	35.0	40.0	31.0	41.0
26	37.052864357648744	39.0	35.0	40.0	32.0	41.0
27	36.96098676149777	39.0	35.0	40.0	32.0	41.0
28	37.066346910518085	39.0	35.0	40.0	32.0	41.0
29	37.20849395850143	39.0	35.0	40.0	33.0	41.0
30	36.95756507187043	39.0	35.0	40.0	32.0	41.0
31	36.885196585283246	39.0	35.0	40.0	32.0	41.0
32	36.77813306238731	39.0	35.0	40.0	31.0	41.0
33	36.69363675303071	39.0	35.0	40.0	31.0	41.0
34	36.67236948271225	39.0	35.0	41.0	31.0	41.0
35	36.547609797886224	39.0	35.0	41.0	31.0	41.0
36	36.38300511012163	39.0	35.0	41.0	30.0	41.0
37	36.318864616641264	39.0	35.0	41.0	30.0	41.0
38	36.198049587106155	39.0	35.0	40.0	30.0	41.0
39	36.15531084082917	39.0	35.0	40.0	30.0	41.0
40	36.03989978981761	39.0	35.0	40.0	29.0	41.0
41	35.94007311558039	39.0	35.0	40.0	29.0	41.0
42	35.9606929046011	39.0	35.0	40.0	29.0	41.0
43	35.849111954497005	39.0	35.0	40.0	28.0	41.0
44	35.73829304007411	39.0	35.0	40.0	27.0	41.0
45	35.785230453535746	39.0	35.0	40.0	27.0	41.0
46	35.71051609240056	39.0	35.0	40.0	27.0	41.0
47	35.66288637201287	39.0	35.0	40.0	27.0	41.0
48	35.63561246750142	39.0	35.0	40.0	27.0	41.0
49	35.65926047674546	39.0	35.0	40.0	27.0	41.0
50	35.54285330066043	39.0	35.0	40.0	27.0	41.0
51	34.586687784518226	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	2.0
12	2.0
13	6.0
14	7.0
15	13.0
16	26.0
17	35.0
18	80.0
19	155.0
20	234.0
21	387.0
22	639.0
23	932.0
24	1291.0
25	1761.0
26	2294.0
27	2756.0
28	3171.0
29	3248.0
30	3691.0
31	4446.0
32	5831.0
33	8050.0
34	12368.0
35	17074.0
36	20225.0
37	27604.0
38	44293.0
39	40144.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.9659823287412	23.320284094870953	26.157248304097063	15.556485272290788
2	31.857574036996084	24.010100708244927	27.0652163085597	17.067108946199287
3	27.16781719112652	23.82233113189692	34.859895008417254	14.149956668559305
4	25.38823974738268	26.249887935929234	32.92143561545588	15.440436701232208
5	18.817300700275926	33.48922690733048	30.121328033947943	17.572144358445644
6	20.75177559294345	33.72182211198438	31.353534749823186	14.172867545248982
7	67.16373307832532	7.75832013467611	18.948789209973206	6.129157577025372
8	64.32178824373189	7.251790534819552	18.78791501060873	9.638506210839832
9	58.868999591588725	9.94879917122394	22.352050523463728	8.830150713723615
10	28.033947942503662	31.278825369313374	27.843688053471993	12.843538634710974
11	18.4337925469922	22.74850830270249	38.40809252009683	20.409606630208486
12	17.742979808544764	20.617298708025782	43.3523593222365	18.28736216119296
13	18.29234278656028	24.807996892089772	42.08728047893693	14.812379842413012
14	13.218579724870255	28.453814660968828	38.86033330344958	19.467272310711333
15	10.955383557959538	26.607994899839625	44.86198687107153	17.574634671129306
16	11.041050314277461	27.336162328541974	39.32303340007371	22.299753957106855
17	11.346362649294244	27.39592983294982	35.71656257159649	25.541144946159438
18	12.211497275597925	26.678221717518852	41.32474673520007	19.785534271683154
19	13.510942433932005	26.460070326430184	38.15159031367978	21.877396925958024
20	13.707677135941188	30.074510155495126	38.19292950422855	18.024883204335136
21	14.751118150394962	28.264550897010626	38.73880604448694	18.245524908107463
22	10.963850621083983	28.063831694707588	35.09996115112213	25.872356533086293
23	11.672593610853779	26.770861349351026	36.530894819153495	25.025650220641705
24	15.826435167199593	25.777724651107192	35.650320254211124	22.745519927482093
25	12.010280010758152	28.78253593521202	33.44788771678172	25.759296337248106
26	11.56202372769925	29.00965245196187	35.40626961121238	24.022054209126498
27	15.204355058821188	26.917789797686996	37.92796023468707	19.94989490880475
28	9.842711850899999	25.976949665800035	38.78562392293976	25.3947145603602
29	12.269770592395583	22.67180667204574	35.972566715476795	29.08585602008188
30	14.973254041777487	25.876839095916882	36.53936188227794	22.61054498002769
31	13.308230981482035	27.134945063702197	32.70477841197741	26.85204554283836
32	12.335016784707488	26.289732938867804	33.36271902300052	28.01253125342418
33	16.220402633754695	23.88060444869458	35.291217165227266	24.607775752323462
34	12.76235444122364	21.138770183984303	35.34500791919433	30.753867455597728
35	13.536841685842074	20.18149398838518	34.82453256830927	31.45713175746347
36	18.09212164679397	21.079998804649914	38.031557242327345	22.79632230622877
37	15.05742661048521	22.122941756566956	39.841018438275114	22.97861319467272
38	14.58327107551624	25.018179282590726	35.03720527149389	25.36134437039915
39	19.684427576726534	20.266662682166373	34.24727808823676	25.801631652870334
40	18.172807777744573	19.931964657482393	35.349988544561654	26.545239020211376
41	16.97845381466097	21.63882497086334	32.32276444630388	29.059956768171812
42	20.212871928199306	20.090348544163206	35.35048660709839	24.346292920539103
43	22.207612387811412	18.607616372311707	36.08014822341093	23.104623016465947
44	17.060634133221768	20.244249868013426	34.196973772026816	28.49814222673799
45	18.083654583669524	17.63240992539023	32.90250923906006	31.381426251880185
46	19.65255157437568	18.762513821235395	36.76398808634412	24.820946518044806
47	14.966281166263236	19.130582035880426	40.51041448764307	25.392722310213273
48	16.10136568747572	18.055265019075794	38.82845730109872	27.01491199234976
49	20.580442080307606	15.085816175078943	40.21854984111805	24.115191903495404
50	18.75653707079461	15.503192580860453	37.37859725667155	28.36167309167339
51	17.42671009771987	15.619241151919036	32.31130900795904	34.64273974240206
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1693.0
1	1871.0
2	2049.0
3	3623.0
4	5197.0
5	3443.5
6	1690.0
7	1624.5
8	1559.0
9	1633.5
10	1708.0
11	1719.0
12	1730.0
13	1863.5
14	1997.0
15	2016.0
16	2035.0
17	1858.0
18	1681.0
19	1790.0
20	1899.0
21	2004.5
22	2110.0
23	2121.0
24	2132.0
25	2719.0
26	3378.5
27	3451.0
28	3226.0
29	3001.0
30	2939.0
31	2877.0
32	3035.0
33	3193.0
34	3322.0
35	3451.0
36	3881.0
37	4311.0
38	4541.5
39	4772.0
40	6013.5
41	7255.0
42	8620.5
43	9986.0
44	12307.0
45	14628.0
46	18821.0
47	23014.0
48	27122.0
49	31230.0
50	29271.5
51	27313.0
52	20541.5
53	13770.0
54	10054.0
55	6338.0
56	4717.0
57	3096.0
58	2532.5
59	1969.0
60	1901.5
61	1834.0
62	1665.0
63	1496.0
64	1292.5
65	1089.0
66	930.5
67	772.0
68	643.5
69	515.0
70	392.5
71	270.0
72	219.5
73	169.0
74	133.0
75	72.5
76	48.0
77	41.5
78	35.0
79	20.5
80	6.0
81	3.5
82	1.0
83	2.0
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	200778.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.73454262917252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.86176118162032	28.865214316309558
2	11.509304770987699	9.376525316518743
3	5.148802973614066	6.2920240265367715
4	2.938155674565329	4.787377103069061
5	1.9966742474262098	4.066680612417695
6	1.481916220380016	3.621910767115919
7	1.0906512116988236	3.109902479355308
8	0.8387743623603061	2.733367201585831
9	0.7739710952974836	2.8374622717628424
>10	3.1680238671655294	20.095329169530526
>50	0.11371139314797153	3.10342766637779
>100	0.06602597021495121	4.673320782157408
>500	0.006113515760643631	1.9748179581428245
>1k	0.006113515760643631	4.462640329119724
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3039	1.5136120491288885	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	2083	1.0374642640129894	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	1652	0.8227993106814493	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT	1130	0.5628106665072866	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	1056	0.5259540387891104	TruSeq Adapter, Index 16 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	963	0.47963422287302393	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	821	0.408909342657064	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCC	813	0.40492484236320714	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	723	0.36009921405731704	No Hit
ACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	645	0.3212503361922123	TruSeq Adapter, Index 13 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	456	0.22711651674984312	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	450	0.22412814152945043	No Hit
ACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG	365	0.1817928259072209	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCG	329	0.1638625745848649	No Hit
AGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG	318	0.15838388668081163	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG	294	0.14643038579924095	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	291	0.14493619818904463	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	254	0.12650788432995647	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	238	0.11853888374224267	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	231	0.11505244598511788	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG	225	0.11206407076472522	No Hit
GACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT	215	0.1070834453974041	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	207	0.1030989451035472	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5936905437846776	0.0
2	0.0	0.0	0.0	3.4635268804351074	0.0
3	0.0	0.0	0.0	4.623514528484196	0.0
4	0.0	0.0	0.0	6.151072328641584	0.0
5	0.0	0.0	0.0	9.822291286893982	0.0
6	0.0	0.0	0.0	11.839444560659036	0.0
7	0.0	0.0	0.0	14.079729850880076	0.0
8	0.0	0.0	0.0	17.36594646823855	0.0
9	0.0	0.0	0.0	18.561794618932353	0.0
10	0.0	0.0	0.0	21.488410084770244	0.0
11	0.0	0.0	0.0	26.28624650111068	0.0
12	4.980625367321121E-4	0.0	0.0	29.794599009851677	0.0
13	4.980625367321121E-4	0.0	0.0	31.117453107412167	0.0
14	4.980625367321121E-4	0.0	0.0	31.585631891940352	0.0
15	4.980625367321121E-4	0.0	0.0	32.52198946099672	0.0
16	4.980625367321121E-4	0.0	0.0	34.80211975415633	0.0
17	4.980625367321121E-4	0.0	0.0	37.82486128958352	0.0
18	4.980625367321121E-4	0.0	0.0	40.936756019085756	0.0
19	4.980625367321121E-4	0.0	0.0	42.67051170945024	0.0
20	4.980625367321121E-4	0.0	0.0	44.21550169839325	0.0
21	4.980625367321121E-4	0.0	0.0	45.96569345246989	0.0
22	4.980625367321121E-4	0.0	0.0	47.85932721712538	0.0
23	4.980625367321121E-4	0.0	0.0	49.42473777007441	0.0
24	4.980625367321121E-4	0.0	0.0	50.5025450995627	0.0
25	4.980625367321121E-4	0.0	0.0	51.332815348295135	0.0
26	4.980625367321121E-4	0.0	0.0	52.02811064957316	0.0
27	4.980625367321121E-4	0.0	0.0	52.602376754425286	0.0
28	4.980625367321121E-4	0.0	0.0	53.20154598611401	0.0
29	4.980625367321121E-4	0.0	0.0	53.75738377710705	0.0
30	4.980625367321121E-4	0.0	0.0	54.44022751496678	0.0
31	4.980625367321121E-4	0.0	0.0	54.94874936497027	0.0
32	4.980625367321121E-4	0.0	0.0	55.42589327515963	0.0
33	4.980625367321121E-4	0.0	0.0	55.81288786620048	0.0
34	4.980625367321121E-4	0.0	0.0	56.16103357937622	0.0
35	4.980625367321121E-4	0.0	0.0	56.606301487214736	0.0
36	4.980625367321121E-4	0.0	0.0	56.93801113667832	0.0
37	4.980625367321121E-4	0.0	0.0	57.2866549123908	0.0
38	4.980625367321121E-4	0.0	0.0	57.60342268575242	0.0
39	4.980625367321121E-4	0.0	0.0	57.92068852165078	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAATT	20	7.0193666E-4	45.0	5
TCGCCGC	20	7.0193666E-4	45.0	28
ATCCTAG	25	3.8792725E-5	45.0	14
TGTCACG	20	7.0193666E-4	45.0	1
ACTATGG	25	3.8792725E-5	45.0	2
AGGTAAG	20	7.0193666E-4	45.0	1
ACTATAT	35	1.2052442E-7	45.0	15
ACCGGGA	20	7.0193666E-4	45.0	4
ATAGGGT	20	7.0193666E-4	45.0	4
CGCGGGA	35	1.2052442E-7	45.0	4
ACGCAGT	20	7.0193666E-4	45.0	2
GACAATA	20	7.0193666E-4	45.0	9
CGCATGG	20	7.0193666E-4	45.0	2
ATGTGAG	20	7.0193666E-4	45.0	1
GGACTCC	20	7.0193666E-4	45.0	8
TAGCGGG	35	1.2052442E-7	45.0	3
TCGGGGT	35	1.2052442E-7	45.0	5
TGAACGG	25	3.8792725E-5	45.0	2
ATGCTAG	25	3.8792725E-5	45.0	1
TAAGTAG	20	7.0193666E-4	45.0	1
>>END_MODULE