SRR2934806.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934806.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	304917
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5419	1.7772049442963167	No Hit
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	2525	0.8280942026846649	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	1672	0.5483459433222811	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCC	1471	0.48242636520758103	No Hit
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	1309	0.4292971529957333	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	1051	0.34468396317686456	TruSeq Adapter, Index 16 (95% over 21bp)
ATAAGAGGAGAATTTTTGCATACATTTTTACTCTTTAAATAAAGACACTTA	1028	0.33714092687518243	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	712	0.23350616725207185	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	682	0.22366742424987784	No Hit
ACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	590	0.19349527904314945	TruSeq Adapter, Index 13 (95% over 21bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	535	0.17545758353912705	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC	527	0.17283391873854195	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG	499	0.1636510919364942	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG	468	0.15348439083422702	No Hit
ACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	455	0.14922093553327628	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	445	0.14594135453254492	No Hit
GCCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	366	0.12003266462676729	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	364	0.11937674842662101	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC	340	0.11150575402486579	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC	339	0.11117779592479266	No Hit
GGCAATGTTTTTCATTATATAATTATTCTATTTTTGTAAATTATATACTAC	318	0.10429067582325682	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGTCA	50	2.1827873E-11	45.000004	6
TACGGGA	50	2.1827873E-11	45.000004	4
ACTAAGG	25	3.8840415E-5	45.000004	2
CAAGCGG	25	3.8840415E-5	45.000004	2
TGCACGG	25	3.8840415E-5	45.000004	2
CAACGAG	20	7.025125E-4	45.0	14
CCGATGG	20	7.025125E-4	45.0	11
GCGCGAC	20	7.025125E-4	45.0	9
GGGTATC	20	7.025125E-4	45.0	7
TAGCGAG	20	7.025125E-4	45.0	1
ATGTAGG	40	6.7866495E-9	45.0	2
ACAGGTC	20	7.025125E-4	45.0	23
ACAATAC	20	7.025125E-4	45.0	17
TAGGCCG	35	1.208009E-7	45.0	1
GGCGATT	20	7.025125E-4	45.0	8
GGTAGCG	20	7.025125E-4	45.0	1
GACTAAG	20	7.025125E-4	45.0	1
CGTTATT	150	0.0	43.5	1
ATAGGGA	135	0.0	43.333336	4
CGTTTTT	2035	0.0	42.7887	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content