##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934806.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	304917
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.624140339830184	31.0	31.0	34.0	30.0	34.0
2	31.718083937596134	31.0	31.0	34.0	30.0	34.0
3	31.74102132711525	33.0	31.0	34.0	30.0	34.0
4	35.56386492061774	37.0	35.0	37.0	33.0	37.0
5	33.08932266813592	37.0	35.0	37.0	28.0	37.0
6	34.06564409331064	37.0	35.0	37.0	28.0	37.0
7	35.343870627088684	37.0	35.0	37.0	32.0	37.0
8	35.29469330998272	37.0	35.0	37.0	33.0	37.0
9	37.279249763050274	39.0	37.0	39.0	34.0	39.0
10	36.80801988737919	39.0	37.0	39.0	32.0	39.0
11	36.605456566869016	39.0	35.0	39.0	32.0	39.0
12	36.869439880360886	39.0	37.0	39.0	33.0	39.0
13	36.785997500959276	39.0	37.0	39.0	33.0	39.0
14	37.59189877901199	39.0	37.0	41.0	32.0	41.0
15	37.76784502012023	39.0	37.0	41.0	33.0	41.0
16	37.77676548044222	40.0	37.0	41.0	33.0	41.0
17	37.78228501526645	39.0	37.0	41.0	33.0	41.0
18	37.7058150250724	39.0	37.0	40.0	33.0	41.0
19	37.52881275888193	39.0	36.0	40.0	33.0	41.0
20	37.35364049888986	39.0	35.0	40.0	33.0	41.0
21	37.27168376968159	39.0	35.0	40.0	33.0	41.0
22	37.270480163454316	39.0	35.0	40.0	33.0	41.0
23	37.04431041890088	39.0	35.0	40.0	32.0	41.0
24	36.74256273018559	38.0	35.0	40.0	31.0	41.0
25	36.55107455471489	38.0	35.0	40.0	31.0	41.0
26	36.80354325931319	38.0	35.0	40.0	32.0	41.0
27	36.724764444094625	38.0	35.0	40.0	32.0	41.0
28	36.79793517580194	39.0	35.0	40.0	32.0	41.0
29	36.83194115119852	39.0	35.0	40.0	32.0	41.0
30	36.58448036678834	39.0	35.0	40.0	31.0	41.0
31	36.44400935336501	39.0	35.0	40.0	31.0	41.0
32	36.256623933726225	39.0	35.0	40.0	30.0	41.0
33	36.09040492986616	39.0	35.0	40.0	30.0	41.0
34	35.952669086997446	39.0	35.0	40.0	29.0	41.0
35	35.73172371497817	39.0	35.0	40.0	27.0	41.0
36	35.543042860844096	39.0	35.0	40.0	26.0	41.0
37	35.580223470649386	39.0	35.0	40.0	26.0	41.0
38	35.53667063495968	39.0	35.0	40.0	26.0	41.0
39	35.47873355700076	39.0	35.0	40.0	25.0	41.0
40	35.38865986481567	39.0	35.0	40.0	25.0	41.0
41	35.21715089680143	38.0	35.0	40.0	24.0	41.0
42	35.23238127096882	38.0	35.0	40.0	24.0	41.0
43	35.15098207053067	38.0	35.0	40.0	24.0	41.0
44	35.098862969267046	38.0	35.0	40.0	23.0	41.0
45	35.121318260379056	38.0	35.0	40.0	23.0	41.0
46	35.020274369746524	38.0	35.0	40.0	23.0	41.0
47	34.91936822151602	38.0	35.0	40.0	23.0	41.0
48	34.8982149240613	38.0	34.0	40.0	23.0	41.0
49	34.902986714417366	38.0	34.0	40.0	23.0	41.0
50	34.82272552858647	38.0	34.0	40.0	23.0	41.0
51	33.8018214792878	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	7.0
10	7.0
11	10.0
12	14.0
13	15.0
14	16.0
15	24.0
16	47.0
17	73.0
18	159.0
19	271.0
20	451.0
21	687.0
22	1157.0
23	1763.0
24	2517.0
25	3636.0
26	4783.0
27	5102.0
28	5278.0
29	5526.0
30	6114.0
31	7403.0
32	9592.0
33	13431.0
34	20136.0
35	24948.0
36	31425.0
37	43313.0
38	64949.0
39	52046.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.38060849345887	21.190028761925376	23.08103516694707	17.348327577668677
2	35.44505553970424	22.505140743218647	24.892675711751068	17.15712800532604
3	27.728529403083463	23.40309002121889	33.4094196125503	15.458960963147348
4	24.682454569604186	24.244958464106624	34.020733511086625	17.051853455202565
5	19.331490208810923	31.206853012459128	31.92606512591951	17.535591652810435
6	20.73187129612321	31.029755638419637	34.289659153146594	13.948713912310563
7	64.57822948540094	7.046507738171372	21.820692188366014	6.554570588061669
8	61.526907322320504	6.7267485906000655	22.71667371776582	9.029670369313617
9	55.26454740142399	9.436338413404304	25.545968247096752	9.753145938074951
10	31.74667204517951	21.779697425856874	31.594499486745576	14.879131042218047
11	21.547503091005094	22.62582932404556	35.63658307014696	20.19008451480239
12	20.476391936166234	19.917879291741684	41.31419369861307	18.29153507347901
13	20.51377915957457	21.627524867422938	42.447288934365744	15.411407038636742
14	15.679676764496566	24.41812034094524	40.43461007421692	19.467592820341274
15	13.397088387987552	23.567069071255457	44.71741490307198	18.31842763768501
16	14.535102995241328	24.80084744373059	39.061777467310776	21.602272093717307
17	15.27071301370537	24.580787558581516	36.874952856023114	23.27354657169
18	16.278856213330183	23.31421337609907	41.454887723544445	18.952042687026307
19	15.237917203698057	25.47676908798132	37.94114463936087	21.34416906895975
20	14.910287061724995	28.16143409518	38.75120114654152	18.17707769655349
21	14.998179832544595	26.717762538658064	40.29949133698679	17.98456629181056
22	13.087495941518512	25.8188293863576	36.414827641620505	24.678847030503384
23	12.914006106579823	24.467969972156357	38.286156560637814	24.331867360626006
24	16.647153159712317	24.484695835260087	36.03538011983589	22.832770885191707
25	13.692578636153446	27.00308608572169	34.498896420993255	24.805438857131612
26	12.950409455687941	27.91644939442537	36.59159705755993	22.541544092326767
27	15.660983152792399	25.213090775522517	36.22985927317926	22.896066798505824
28	11.584463968883336	24.65228242439746	35.44079208440329	28.322461522315912
29	14.049397049033017	25.278354437437073	34.1735619857207	26.498686527809205
30	16.64452949491173	25.492183118684757	32.41078719782761	25.452500188575904
31	13.846718943187819	28.131261949973275	28.936727043752892	29.085292063086023
32	14.374075568105418	27.12574241514904	30.087859975009597	28.412322041735948
33	15.098862969267046	24.91530482065611	30.352522161768615	29.633310048308225
34	13.306899910467438	23.93667785003788	32.110377578160616	30.646044661334066
35	12.86874788876973	26.616751443835536	31.216035839261174	29.298464828133557
36	14.965711980637353	28.004670123345043	30.153451595024222	26.876166300993386
37	13.52761571181666	25.273435065935978	31.03270726132029	30.16624196092707
38	14.192386780664904	25.48693578908359	30.697862041145623	29.622815389105888
39	17.05152549710249	23.4358858312262	32.768589485007396	26.743999186663913
40	17.70317824194781	22.128644844334687	34.76487044015257	25.403306473564935
41	14.889297743320313	26.340938681674032	30.38728572037637	28.38247785462929
42	16.22638291731848	26.20516402824375	32.33699662531115	25.231456429126613
43	18.170190576451954	24.072124545368084	31.39870850100191	26.35897637717805
44	17.28044025095354	21.587513979214016	30.213139969237528	30.918905800594914
45	18.411239780005705	19.60500726427192	30.214779759737898	31.76897319598448
46	19.795222962314334	20.974888248277402	32.91223513283943	26.317653656568833
47	13.295093418864806	23.618230534866864	36.67686616357894	26.409809882689387
48	15.114604958070558	21.59407314121548	34.64516573362587	28.646156167088094
49	18.835617561500342	18.709353692972186	37.00416834745193	25.45086039807554
50	17.398177208879794	17.61036609962711	35.63559919584674	29.355857495646354
51	16.016161775171604	18.527008989331524	31.45544525231456	34.00138398318231
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1185.0
1	2479.0
2	3773.0
3	6651.0
4	9529.0
5	6320.0
6	3111.0
7	3021.5
8	2932.0
9	3023.0
10	3114.0
11	3091.5
12	3069.0
13	3175.0
14	3281.0
15	3025.0
16	2769.0
17	2769.5
18	2770.0
19	2678.5
20	2587.0
21	2769.5
22	2952.0
23	3010.5
24	3069.0
25	2782.5
26	2682.0
27	2868.0
28	3200.0
29	3532.0
30	3831.0
31	4130.0
32	4233.0
33	4336.0
34	4727.5
35	5119.0
36	5297.0
37	5475.0
38	6297.0
39	7119.0
40	7811.5
41	8504.0
42	10874.0
43	13244.0
44	15816.5
45	18389.0
46	24712.5
47	31036.0
48	36634.5
49	42233.0
50	41735.0
51	41237.0
52	32723.5
53	24210.0
54	19178.5
55	14147.0
56	11355.5
57	8564.0
58	7309.5
59	6055.0
60	5420.0
61	4785.0
62	4417.0
63	4049.0
64	3572.5
65	3096.0
66	2617.5
67	2139.0
68	1812.5
69	1486.0
70	1218.0
71	950.0
72	757.5
73	565.0
74	539.5
75	373.5
76	233.0
77	190.5
78	148.0
79	107.0
80	66.0
81	43.0
82	20.0
83	13.0
84	6.0
85	8.0
86	10.0
87	7.5
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	1.5
94	3.0
95	4.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	304917.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.065582443329596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.10453245248202	32.944983342183924
2	10.87316335911774	9.800108795602222
3	4.820546624602879	6.517222239988657
4	2.88146889213072	5.194202956648262
5	1.9242124165443582	4.335787664812912
6	1.4220363683623858	3.845093831750887
7	1.1140948708582754	3.514513397664797
8	0.8521057887544512	3.072051493884169
9	0.63734745926372	2.5850191023446243
>10	2.233508117541574	15.461911872217218
>50	0.07471835473193007	2.306549930013192
>100	0.053474900935597015	4.638519116447026
>500	0.003662664447643631	1.0055461695749512
>1k	0.004395197337172358	2.9895686512379376
>5k	7.325328895287262E-4	1.7889214356292384
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5419	1.7772049442963167	No Hit
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	2525	0.8280942026846649	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	1672	0.5483459433222811	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCC	1471	0.48242636520758103	No Hit
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	1309	0.4292971529957333	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	1051	0.34468396317686456	TruSeq Adapter, Index 16 (95% over 21bp)
ATAAGAGGAGAATTTTTGCATACATTTTTACTCTTTAAATAAAGACACTTA	1028	0.33714092687518243	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	712	0.23350616725207185	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	682	0.22366742424987784	No Hit
ACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	590	0.19349527904314945	TruSeq Adapter, Index 13 (95% over 21bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	535	0.17545758353912705	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC	527	0.17283391873854195	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG	499	0.1636510919364942	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG	468	0.15348439083422702	No Hit
ACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	455	0.14922093553327628	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	445	0.14594135453254492	No Hit
GCCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	366	0.12003266462676729	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	364	0.11937674842662101	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC	340	0.11150575402486579	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC	339	0.11117779592479266	No Hit
GGCAATGTTTTTCATTATATAATTATTCTATTTTTGTAAATTATATACTAC	318	0.10429067582325682	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4571735915019497	0.0
2	0.0	0.0	0.0	2.493793392956116	0.0
3	0.0	0.0	0.0	3.3543554475480213	0.0
4	0.0	0.0	0.0	4.584854239022422	0.0
5	0.0	0.0	0.0	7.9595430887749785	0.0
6	0.0	0.0	0.0	9.84825378709616	0.0
7	0.0	0.0	0.0	12.022943948681117	0.0
8	0.0	0.0	0.0	14.955545279535087	0.0
9	0.0	0.0	0.0	16.085688892387108	0.0
10	0.0	0.0	0.0	19.04649461984737	0.0
11	0.0	0.0	0.0	23.59363367736138	0.0
12	0.0	0.0	0.0	26.918800854002892	0.0
13	0.0	0.0	0.0	28.19521377948753	0.0
14	0.0	0.0	0.0	28.764549041214494	0.0
15	0.0	0.0	0.0	29.835988154153426	0.0
16	0.0	0.0	0.0	32.009694441438164	0.0
17	0.0	0.0	0.0	34.305073183850034	0.0
18	0.0	0.0	0.0	36.64702197647229	0.0
19	0.0	0.0	0.0	38.11856997150044	0.0
20	0.0	0.0	0.0	39.634720268138544	0.0
21	0.0	0.0	0.0	41.312225950012625	0.0
22	0.0	0.0	0.0	42.91922064037099	0.0
23	0.0	0.0	0.0	44.29697261877823	0.0
24	0.0	0.0	0.0	45.31856210050604	0.0
25	0.0	0.0	0.0	46.046629082668396	0.0
26	0.0	0.0	0.0	46.749443291125125	0.0
27	0.0	0.0	0.0	47.38305834046642	0.0
28	0.0	0.0	0.0	47.89073747937964	0.0
29	0.0	0.0	0.0	48.389561749590875	0.0
30	0.0	0.0	0.0	48.911015128707156	0.0
31	0.0	0.0	0.0	49.308500345995796	0.0
32	0.0	0.0	0.0	49.72631896548897	0.0
33	0.0	0.0	0.0	50.05263727506174	0.0
34	0.0	0.0	0.0	50.429789090145846	0.0
35	0.0	0.0	0.0	50.786607503025415	0.0
36	0.0	0.0	0.0	51.05815680988597	0.0
37	0.0	0.0	0.0	51.35167930945143	0.0
38	0.0	0.0	0.0	51.645529767116955	0.0
39	0.0	0.0	0.0	52.02136974980077	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGTCA	50	2.1827873E-11	45.000004	6
TACGGGA	50	2.1827873E-11	45.000004	4
ACTAAGG	25	3.8840415E-5	45.000004	2
CAAGCGG	25	3.8840415E-5	45.000004	2
TGCACGG	25	3.8840415E-5	45.000004	2
CAACGAG	20	7.025125E-4	45.0	14
CCGATGG	20	7.025125E-4	45.0	11
GCGCGAC	20	7.025125E-4	45.0	9
GGGTATC	20	7.025125E-4	45.0	7
TAGCGAG	20	7.025125E-4	45.0	1
ATGTAGG	40	6.7866495E-9	45.0	2
ACAGGTC	20	7.025125E-4	45.0	23
ACAATAC	20	7.025125E-4	45.0	17
TAGGCCG	35	1.208009E-7	45.0	1
GGCGATT	20	7.025125E-4	45.0	8
GGTAGCG	20	7.025125E-4	45.0	1
GACTAAG	20	7.025125E-4	45.0	1
CGTTATT	150	0.0	43.5	1
ATAGGGA	135	0.0	43.333336	4
CGTTTTT	2035	0.0	42.7887	1
>>END_MODULE