##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934804.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 152505 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.784780826858135 31.0 31.0 34.0 30.0 34.0 2 31.889964263466773 33.0 31.0 34.0 30.0 34.0 3 31.866797809907872 33.0 31.0 34.0 30.0 34.0 4 35.66356512901216 37.0 35.0 37.0 33.0 37.0 5 33.243769056752235 37.0 35.0 37.0 30.0 37.0 6 34.22043211697977 37.0 35.0 37.0 28.0 37.0 7 35.43866758466935 37.0 35.0 37.0 32.0 37.0 8 35.38565948657421 37.0 35.0 37.0 33.0 37.0 9 37.47021409134127 39.0 37.0 39.0 35.0 39.0 10 36.97070259991476 39.0 37.0 39.0 33.0 39.0 11 36.789082325169666 39.0 37.0 39.0 33.0 39.0 12 37.0085636536507 39.0 37.0 39.0 33.0 39.0 13 36.899341005212946 39.0 37.0 39.0 33.0 39.0 14 37.902462214353626 40.0 37.0 41.0 33.0 41.0 15 38.087852857283366 40.0 37.0 41.0 33.0 41.0 16 38.052686797154195 40.0 37.0 41.0 33.0 41.0 17 38.00363266778138 40.0 37.0 41.0 33.0 41.0 18 37.852129438379066 39.0 37.0 40.0 33.0 41.0 19 37.61338972492705 39.0 36.0 40.0 33.0 41.0 20 37.43346119799351 39.0 35.0 40.0 33.0 41.0 21 37.31278318743648 39.0 35.0 40.0 33.0 41.0 22 37.23003835939805 39.0 35.0 40.0 33.0 41.0 23 36.9900331136684 39.0 35.0 40.0 32.0 41.0 24 36.716828956427655 38.0 35.0 40.0 32.0 41.0 25 36.534198878725284 38.0 35.0 40.0 31.0 41.0 26 36.769823940198684 38.0 35.0 40.0 32.0 41.0 27 36.703963804465424 38.0 35.0 40.0 32.0 41.0 28 36.76506999770499 38.0 35.0 40.0 32.0 41.0 29 36.79771810760303 39.0 35.0 40.0 33.0 41.0 30 36.53184485754566 38.0 35.0 40.0 31.0 41.0 31 36.35328022032065 38.0 35.0 40.0 31.0 41.0 32 35.9973443493656 38.0 35.0 40.0 30.0 41.0 33 35.801783548080394 38.0 35.0 40.0 29.0 41.0 34 35.648719714107735 39.0 35.0 40.0 27.0 41.0 35 35.33695944395266 38.0 35.0 40.0 25.0 41.0 36 35.18060391462575 38.0 35.0 40.0 24.0 41.0 37 35.069119045277205 38.0 35.0 40.0 23.0 41.0 38 34.86582734992295 38.0 35.0 40.0 23.0 41.0 39 34.83473984459526 38.0 35.0 40.0 22.0 41.0 40 34.69580669486246 38.0 35.0 40.0 21.0 41.0 41 34.44091669125602 38.0 34.0 40.0 20.0 41.0 42 34.50546539457723 38.0 34.0 40.0 20.0 41.0 43 34.4387265991279 38.0 34.0 40.0 20.0 41.0 44 34.37630897347628 38.0 34.0 40.0 19.0 41.0 45 34.330867840398675 38.0 34.0 40.0 20.0 41.0 46 34.194826399134456 38.0 34.0 40.0 20.0 41.0 47 34.08727582702206 38.0 34.0 40.0 20.0 41.0 48 34.00232123536933 37.0 33.0 40.0 20.0 41.0 49 34.001632733353006 37.0 34.0 40.0 18.0 41.0 50 33.86380118684633 37.0 33.0 40.0 18.0 41.0 51 32.80377036818465 36.0 32.0 39.0 15.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 6.0 10 3.0 11 3.0 12 8.0 13 6.0 14 6.0 15 15.0 16 30.0 17 42.0 18 84.0 19 166.0 20 315.0 21 467.0 22 713.0 23 1024.0 24 1447.0 25 2068.0 26 2789.0 27 3127.0 28 3165.0 29 2939.0 30 3242.0 31 3736.0 32 4579.0 33 5956.0 34 10258.0 35 14247.0 36 15061.0 37 19721.0 38 29947.0 39 27320.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.72994328054818 21.578964624110686 22.52778597423035 19.163306121110782 2 37.67876463066785 23.9572473033671 23.782171076358154 14.5818169896069 3 26.955837513524145 24.510671781253073 35.171305858824304 13.362184846398478 4 25.052949083636598 26.295531294055934 33.50644241172421 15.14507721058326 5 18.306285039834762 33.2166158486607 33.12678272843513 15.350316383069407 6 20.55539162650405 33.54250680305564 33.93987082390741 11.962230746532901 7 63.94151011442247 9.581325202452378 21.793383823481197 4.683780859643946 8 62.17697780400643 9.040359332480902 22.317301072095997 6.465361791416675 9 57.15484738205304 10.641618307596472 25.007704665420803 7.195829644929674 10 30.563588079079373 25.435887347955806 30.854070358348906 13.146454214615913 11 23.641847808268583 24.678535129995737 33.54381823546769 18.13579882626799 12 21.05439165928986 22.675977836792235 40.022950067210914 16.246680436706992 13 20.066883053014656 25.925051637651226 39.90098685289007 14.10707845644405 14 14.89721648470542 28.99904921150126 38.94364119209206 17.160093111701254 15 13.453985115242123 26.00832759581653 43.72315661781581 16.81453067112554 16 12.97859086587325 26.555850627848265 40.70817350250812 19.757385003770366 17 13.904462148782008 25.362447132880888 37.19222320579653 23.540867512540572 18 14.076915510966852 26.104717878102356 41.85174256581751 17.966624045113274 19 16.122750073768074 26.70141962558605 38.687256155535884 18.488574145109997 20 16.904363791351106 28.866594537884005 37.6348316448641 16.59421002590079 21 15.872922199272155 27.889577390905217 39.33313661847153 16.904363791351106 22 13.916920756696502 28.056785023441854 35.15032294023147 22.875971279630175 23 14.10707845644405 26.94665748663978 36.6801088488902 22.266155208025967 24 15.655880135077538 26.343398577095833 36.92010098029573 21.0806203075309 25 13.6926658142356 28.09416084718534 35.8840693747746 22.329103963804464 26 12.404183469394447 30.099341005212942 37.90629815415888 19.59017737123373 27 15.499819678043341 28.219402642536313 36.35028359725911 19.93049408216124 28 13.214648700042622 27.12370086226681 37.47680403921183 22.18484639847874 29 14.84082489098718 24.895577194190356 39.11150454083473 21.15209337398774 30 16.819776400773744 27.602373692665815 33.372676305694895 22.205173600865546 31 19.26297498442674 29.546572243533 30.69341988787253 20.49703288416773 32 16.501098324645096 29.528212189764268 32.60811120946855 21.362578276122093 33 18.029572800891774 26.388642995311628 31.621914035605393 23.95987016819121 34 18.211206189960986 25.17819087898757 33.802826136847976 22.80777679420347 35 16.360119340349495 25.92242877282712 33.60348841021606 24.113963476607324 36 18.946919773122193 26.146027999082 32.478935116881416 22.428117110914396 37 21.709452149109865 25.87980721943543 33.32546473886102 19.085275892593685 38 17.680731779285924 28.27776138487263 32.39238057768598 21.64912625815547 39 21.09898036129963 23.28185961116029 33.34120192780565 22.277958099734434 40 21.745516540441297 24.871971410773416 32.44090357693191 20.94160847185338 41 19.289859348873808 26.978131864529033 30.930133438247925 22.801875348349235 42 18.735123438575783 25.755876856496506 34.89197075505721 20.617028949870498 43 20.67538769220681 22.340251139306908 35.16147011573391 21.822891052752368 44 19.632143208419393 23.41693714960165 32.53729385921773 24.41362578276122 45 21.516015868332186 22.094357562047147 31.500606537490572 24.889020032130095 46 21.67076489295433 24.624766401101603 33.06580112127471 20.638667584669353 47 18.059735746368972 24.816235533261207 36.611258647257465 20.512770073112357 48 17.4584439854431 23.809711157011247 36.26504049047572 22.46680436706993 49 20.59473459886561 20.390151142585488 37.54893282187469 21.46618143667421 50 18.539720009180026 21.394052654011343 35.15884725090981 24.907380085898822 51 18.276777810563587 20.993410052129438 31.46650929477722 29.263302842529754 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1331.0 1 1965.0 2 2599.0 3 4306.0 4 6013.0 5 3937.5 6 1862.0 7 1842.0 8 1822.0 9 1935.0 10 2048.0 11 2292.0 12 2536.0 13 2244.5 14 1953.0 15 1932.5 16 1912.0 17 1687.5 18 1463.0 19 1596.5 20 1730.0 21 1620.5 22 1511.0 23 1504.5 24 1498.0 25 2160.5 26 2588.5 27 2354.0 28 2236.5 29 2119.0 30 2261.0 31 2403.0 32 2472.5 33 2542.0 34 2573.5 35 2605.0 36 2686.5 37 2768.0 38 3015.5 39 3263.0 40 3949.0 41 4635.0 42 5881.5 43 7128.0 44 8133.5 45 9139.0 46 11019.0 47 12899.0 48 15495.0 49 18091.0 50 17841.0 51 17591.0 52 14430.5 53 11270.0 54 8773.5 55 6277.0 56 5199.5 57 4122.0 58 3588.0 59 3054.0 60 2811.0 61 2568.0 62 2396.5 63 2225.0 64 1831.0 65 1437.0 66 1275.5 67 1114.0 68 873.0 69 632.0 70 529.0 71 426.0 72 371.0 73 316.0 74 265.5 75 174.0 76 133.0 77 81.0 78 29.0 79 28.0 80 27.0 81 19.0 82 11.0 83 9.5 84 8.0 85 5.5 86 3.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 152505.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.204419527228616 #Duplication Level Percentage of deduplicated Percentage of total 1 74.69356967754102 33.76479459689846 2 10.177113099986945 9.20100980295728 3 4.257386965288154 5.7735811940592106 4 2.407925847488359 4.3539556080128525 5 1.6913503241996548 3.8228254811317663 6 1.2025123660047288 3.261532408773483 7 1.056006034320196 3.341529785908659 8 0.8688840859310406 3.1421920592767454 9 0.6991688304152947 2.8444969017409267 >10 2.825686476450195 18.7279105603095 >50 0.0768795601908934 2.2884495590308513 >100 0.030461712528467192 3.079243303498246 >500 0.010153904176155732 3.338906921084554 >1k 0.0029011154789016376 3.059571817317465 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3607 2.3651683551358973 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCC 1059 0.6944034621815678 No Hit GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA 961 0.6301432739910167 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 936 0.6137503688403658 No Hit CCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC 898 0.5888331530113766 No Hit GCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC 745 0.4885085734893938 No Hit CTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGCT 536 0.35146388642995313 No Hit TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 512 0.33572669748532835 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 504 0.3304809678371201 No Hit GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 415 0.2721222255008033 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 397 0.2603193337923347 No Hit TTAGTTTTTTTTTTTATATTTGTTTTACTATTACTAGTATAACTATTTTAT 357 0.23409068555129342 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 339 0.22228779384282482 No Hit CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCG 339 0.22228779384282482 No Hit TCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC 338 0.22163207763679882 No Hit CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGC 258 0.16917478115471624 No Hit ACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC 256 0.16786334874266418 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 246 0.16130618668240387 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 244 0.15999475427035179 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 179 0.11737320087865971 No Hit CGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTG 163 0.1068817415822432 No Hit AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 158 0.10360316055211304 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.38228254811317663 0.0 2 0.0 0.0 0.0 1.9428871184551326 0.0 3 0.0 0.0 0.0 2.644503458902987 0.0 4 0.0 0.0 0.0 3.4589029867873187 0.0 5 0.0 0.0 0.0 5.767024031998951 0.0 6 0.0 0.0 0.0 7.083702173699223 0.0 7 0.0 0.0 0.0 8.509229205599816 0.0 8 0.0 0.0 0.0 10.594406740762597 0.0 9 0.0 0.0 0.0 11.473066456837481 0.0 10 0.0 0.0 0.0 13.945116553555621 0.0 11 0.0 0.0 0.0 18.077440083931673 0.0 12 0.0 0.0 0.0 20.79800662273368 0.0 13 0.0 0.0 0.0 21.851086849611487 0.0 14 0.0 0.0 0.0 22.308776761417658 0.0 15 0.0 0.0 0.0 23.186780761286514 0.0 16 0.0 0.0 0.0 24.932953017933837 0.0 17 0.0 0.0 0.0 27.016819120684566 0.0 18 0.0 0.0 0.0 29.26920428838399 0.0 19 0.0 0.0 0.0 30.461296350939314 0.0 20 0.0 0.0 0.0 31.65010983246451 0.0 21 0.0 0.0 0.0 32.92744500180322 0.0 22 0.0 0.0 0.0 34.278220386216844 0.0 23 0.0 0.0 0.0 35.55227697452543 0.0 24 0.0 0.0 0.0 36.37585652929412 0.0 25 0.0 0.0 0.0 36.96075538506934 0.0 26 0.0 0.0 0.0 37.53385134913609 0.0 27 0.0 0.0 0.0 37.97121405855546 0.0 28 0.0 0.0 0.0 38.41447821382906 0.0 29 0.0 0.0 0.0 38.842005180158026 0.0 30 0.0 0.0 0.0 39.29182649749188 0.0 31 0.0 0.0 0.0 39.720009180026885 0.0 32 0.0 0.0 0.0 40.04917871545195 0.0 33 0.0 0.0 0.0 40.414412642208454 0.0 34 0.0 0.0 0.0 40.72653355627684 0.0 35 0.0 0.0 0.0 41.062915969968195 0.0 36 0.0 0.0 0.0 41.38618405953903 0.0 37 0.0 0.0 0.0 41.724533621848465 0.0 38 0.0 0.0 0.0 42.08321038654471 0.0 39 0.0 0.0 0.0 42.410412773351695 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACGAG 20 7.014034E-4 45.000004 14 TCGGGAG 20 7.014034E-4 45.000004 45 ACACAAC 20 7.014034E-4 45.000004 11 AGGGTCC 20 7.014034E-4 45.000004 6 AGGGTAC 40 6.7484507E-9 45.000004 6 TAGTTGG 20 7.014034E-4 45.000004 2 TACATGG 20 7.014034E-4 45.000004 2 GGCCATG 20 7.014034E-4 45.000004 1 TACATAG 20 7.014034E-4 45.000004 1 AATGCTT 20 7.014034E-4 45.000004 31 GGGGTTA 20 7.014034E-4 45.000004 7 GCGGGTG 20 7.014034E-4 45.000004 5 TATTAGG 20 7.014034E-4 45.000004 1 CGGGAAC 20 7.014034E-4 45.000004 6 TCGTAGG 20 7.014034E-4 45.000004 2 ACGAGCG 20 7.014034E-4 45.000004 16 GATTAGG 20 7.014034E-4 45.000004 2 TCGAAGG 20 7.014034E-4 45.000004 2 TAGTATG 20 7.014034E-4 45.000004 1 CTAGCGG 20 7.014034E-4 45.000004 2 >>END_MODULE