##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934801.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	121585
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76430480733643	31.0	31.0	34.0	30.0	34.0
2	31.877838549163137	33.0	31.0	34.0	30.0	34.0
3	31.8843525105893	33.0	31.0	34.0	30.0	34.0
4	35.651190525147015	37.0	35.0	37.0	33.0	37.0
5	33.24683965949747	37.0	35.0	37.0	30.0	37.0
6	34.22810379569848	37.0	35.0	37.0	28.0	37.0
7	35.44997326972899	37.0	35.0	37.0	32.0	37.0
8	35.433071513755806	37.0	35.0	37.0	33.0	37.0
9	37.50507875149073	39.0	37.0	39.0	35.0	39.0
10	36.95559485133857	39.0	37.0	39.0	33.0	39.0
11	36.760217132047536	39.0	37.0	39.0	32.0	39.0
12	37.00065797590163	39.0	37.0	39.0	33.0	39.0
13	36.90130361475511	39.0	37.0	39.0	33.0	39.0
14	37.904593494263274	40.0	37.0	41.0	33.0	41.0
15	38.10843442858905	40.0	37.0	41.0	33.0	41.0
16	38.08944359912818	40.0	37.0	41.0	33.0	41.0
17	38.00518156022536	40.0	37.0	41.0	33.0	41.0
18	37.85434058477608	39.0	37.0	40.0	33.0	41.0
19	37.54871900316651	39.0	36.0	40.0	33.0	41.0
20	37.3393263971707	39.0	35.0	40.0	33.0	41.0
21	37.21035489575194	39.0	35.0	40.0	33.0	41.0
22	37.16440350372168	39.0	35.0	40.0	33.0	41.0
23	36.938742443558006	39.0	35.0	40.0	32.0	41.0
24	36.660854546202245	38.0	35.0	40.0	32.0	41.0
25	36.44735781552001	38.0	35.0	40.0	31.0	41.0
26	36.68436073528807	38.0	35.0	40.0	32.0	41.0
27	36.581691820537074	38.0	35.0	40.0	32.0	41.0
28	36.61800386560842	38.0	35.0	40.0	32.0	41.0
29	36.6013077271045	38.0	35.0	40.0	32.0	41.0
30	36.32558292552535	38.0	35.0	40.0	31.0	41.0
31	36.11875642554592	38.0	35.0	40.0	31.0	41.0
32	35.735666406217874	38.0	35.0	40.0	30.0	41.0
33	35.481580787103674	38.0	35.0	40.0	27.0	41.0
34	35.26013899740922	38.0	35.0	40.0	25.0	41.0
35	34.952124028457455	38.0	35.0	40.0	23.0	41.0
36	34.80031253855328	38.0	35.0	40.0	22.0	41.0
37	34.683661635892584	38.0	35.0	40.0	21.0	41.0
38	34.56898466093679	38.0	34.0	40.0	21.0	41.0
39	34.50957766171814	38.0	35.0	40.0	20.0	41.0
40	34.40263190360653	38.0	34.0	40.0	19.0	41.0
41	34.22478101739524	38.0	34.0	40.0	18.0	41.0
42	34.25607599621664	38.0	34.0	40.0	18.0	41.0
43	34.10584364847637	38.0	34.0	40.0	18.0	41.0
44	33.992655343998024	38.0	34.0	40.0	17.0	41.0
45	33.97123822839988	38.0	34.0	40.0	18.0	41.0
46	33.89224822140889	37.0	34.0	40.0	17.0	41.0
47	33.79573138133816	37.0	33.0	40.0	17.0	41.0
48	33.735666406217874	37.0	33.0	40.0	17.0	41.0
49	33.72746638154378	37.0	34.0	40.0	15.0	41.0
50	33.61686063247934	37.0	33.0	40.0	15.0	41.0
51	32.71954599662787	36.0	32.0	40.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	3.0
9	3.0
10	2.0
11	4.0
12	4.0
13	10.0
14	3.0
15	17.0
16	16.0
17	38.0
18	82.0
19	162.0
20	249.0
21	419.0
22	625.0
23	935.0
24	1364.0
25	2062.0
26	2425.0
27	2761.0
28	2544.0
29	2479.0
30	2598.0
31	2732.0
32	3482.0
33	4865.0
34	8161.0
35	11537.0
36	12212.0
37	15126.0
38	23106.0
39	21551.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.711518690627955	21.23370481556113	23.62380227824156	20.430974215569357
2	37.9051692231772	23.589258543405847	24.155117818809885	14.350454414607064
3	25.166755767570017	23.08837438828803	38.625652835464905	13.119217008677056
4	23.435456676399227	24.526051733355267	37.59016326027059	14.448328329974917
5	17.62964181436855	32.40284574577456	35.47230332689065	14.495209112966236
6	20.676070238927498	32.16761936094091	35.19184109881975	11.964469301311839
7	64.24065468602213	8.407287083110582	22.554591438088583	4.797466792778715
8	63.218324628860465	7.557675700127482	22.908253485216104	6.315746185795945
9	58.64621458239092	9.561212320598758	25.187317514496034	6.60525558251429
10	30.88785623226549	24.260393963071103	31.517045688201666	13.334704116461735
11	21.775712464530987	25.103425587037876	35.21733766500802	17.90352428342312
12	19.73927704897808	22.033145536044742	41.57009499527079	16.65748241970638
13	18.550808076654192	25.792655343998028	41.04453674384176	14.611999835506023
14	14.250935559485134	28.21236172225192	39.45634741127606	18.08035530698688
15	12.482625323847515	26.4843525105893	44.05313155405683	16.979890611506356
16	14.384998149442776	25.64378829625365	40.3734013241765	19.597812230127072
17	13.500843031623965	25.819796849940367	38.097627174404735	22.581732944030925
18	14.194185137969322	26.0081424517827	41.79709668133405	18.00057572891393
19	15.18608380968047	27.505037627996877	38.78521199161081	18.523666570711846
20	16.858987539581364	28.01250154213102	38.8214006662006	16.307110252087018
21	15.239544351688119	28.423736480651396	39.20467162890159	17.132047538758894
22	13.521404778549986	28.251840276349878	36.066949047991116	22.15980589710902
23	13.379939959698975	27.80441666323971	36.90093350331044	21.914709873750873
24	16.070238927499283	25.272031911831228	38.37562199284451	20.28210716782498
25	13.355265863387752	28.76506147962331	35.92877410864827	21.950898548340668
26	14.72632314841469	29.53242587490233	37.6469136817864	18.094337294896572
27	15.46901344738249	28.352181601348853	38.28350536661595	17.89529958465271
28	14.532220257433071	26.843771846856107	38.59275404038327	20.03125385532755
29	16.15577579471152	26.070650162437804	38.20948307768228	19.5640909651684
30	18.09022494551137	27.041987087222928	37.666652958835385	17.201135008430317
31	18.719414401447548	29.446889007690093	32.02121972282765	19.812476868034707
32	18.54916313690011	31.25221038779455	31.236583460130774	18.962043015174572
33	19.272936628695973	27.234445038450467	33.43915779084591	20.05346054200765
34	18.700497594275607	26.857753834765802	31.709503639429204	22.732244931529383
35	18.579594522350618	28.237035818563143	32.21532261380927	20.968047045276965
36	22.122794752642186	26.76810461816836	33.78541760908006	17.32368302010939
37	19.42015873668627	29.267590574495213	33.76403339227701	17.548217296541512
38	18.16424723444504	31.588600567504216	30.59423448616194	19.6529177118888
39	19.752436567010733	27.294485339474438	33.35197598387959	19.601102109635235
40	19.70555578401941	25.879014681087302	33.46383188715713	20.95159764773615
41	19.096105605132212	28.256775095612124	29.935436114652305	22.711683184603366
42	23.14265740017272	25.955504379652094	31.42904141135831	19.472796808816877
43	22.974873545256404	25.243245466134802	33.0756261051939	18.706254883414893
44	20.660443311263723	25.863387753423535	31.56968376033228	21.906485174980467
45	20.42521692643007	25.336184562240412	31.16831846033639	23.070280050993134
46	20.957354936875436	25.581280585598552	34.189250318707074	19.272114158818933
47	17.877205247357818	26.946580581486202	35.350577785088625	19.825636386067362
48	18.742443558004688	24.512892215322612	35.13344573754986	21.611218489122834
49	21.295390056339187	22.187769872928403	36.15084097544927	20.365999095283136
50	18.74491096763581	21.979684994037093	35.422132664391164	23.85327137393593
51	17.817987416210883	22.725665172513057	31.56968376033228	27.88666365094378
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1233.0
1	1692.0
2	2151.0
3	3885.0
4	5619.0
5	3699.5
6	1780.0
7	1775.0
8	1770.0
9	1947.5
10	2125.0
11	2026.5
12	1928.0
13	1924.5
14	1921.0
15	1871.5
16	1822.0
17	1635.5
18	1449.0
19	1414.5
20	1380.0
21	1370.0
22	1360.0
23	1180.0
24	1000.0
25	1117.0
26	1256.0
27	1278.0
28	1368.0
29	1458.0
30	1556.0
31	1654.0
32	1936.5
33	2219.0
34	2324.0
35	2429.0
36	2433.5
37	2438.0
38	2616.0
39	2794.0
40	3444.0
41	4094.0
42	5352.0
43	6610.0
44	7086.5
45	7563.0
46	8911.0
47	10259.0
48	11940.0
49	13621.0
50	13379.0
51	13137.0
52	10505.5
53	7874.0
54	6168.0
55	4462.0
56	3846.0
57	3230.0
58	2851.0
59	2472.0
60	2269.0
61	2066.0
62	1837.0
63	1608.0
64	1427.5
65	1247.0
66	1027.5
67	808.0
68	692.5
69	577.0
70	437.5
71	298.0
72	277.5
73	257.0
74	196.0
75	121.0
76	107.0
77	80.5
78	54.0
79	50.0
80	46.0
81	26.5
82	7.0
83	5.5
84	4.0
85	5.0
86	6.0
87	3.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	121585.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.958095159764774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.15336112376082	35.76016778385492
2	9.333730339440221	8.765883949500349
3	3.879567029810488	5.465312332935806
4	2.18061442533366	4.0958999876629525
5	1.613129225487792	3.7874737837726693
6	1.2260482712719374	3.4543734835711644
7	1.0981889515535783	3.6098202903318666
8	0.9072757207412339	3.408315170456882
9	0.7268714751112201	3.071924990747214
>10	2.790135565908852	18.500637414154706
>50	0.05604792097243143	1.7962742114570056
>100	0.02627246295582723	2.9164781839865115
>500	0.0070059901215539285	2.611341859604392
>1k	0.0017514975303884821	2.7560965579635646
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3351	2.7560965579635646	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCC	943	0.7755890940494304	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	879	0.7229510219188222	No Hit
GCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	688	0.5658592754040384	No Hit
CCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	665	0.546942468232101	No Hit
CTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGCT	415	0.34132499897191265	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	360	0.2960891557346712	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	333	0.27388246905457087	No Hit
TCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	302	0.24838590286630752	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	291	0.23933873421885923	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCG	276	0.22700168606324794	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	228	0.18752313196529177	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGC	225	0.1850557223341695	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	224	0.18423325245712877	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	198	0.16284903565406916	No Hit
ACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	194	0.15955915614590616	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG	142	0.11679072253978698	No Hit
GCCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG	130	0.10692108401529794	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGT	124	0.10198626475305342	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.36599909528313523	0.0
2	0.0	0.0	0.0	1.9722827651437267	0.0
3	0.0	0.0	0.0	2.6360159559156147	0.0
4	0.0	0.0	0.0	3.551424929061973	0.0
5	0.0	0.0	0.0	5.787720524735781	0.0
6	0.0	0.0	0.0	7.035407328206604	0.0
7	0.0	0.0	0.0	8.42867129991364	0.0
8	0.0	0.0	0.0	10.499650450302259	0.0
9	0.0	0.0	0.0	11.291688941892502	0.0
10	0.0	0.0	0.0	13.388164658469384	0.0
11	0.0	0.0	0.0	16.927252539375747	0.0
12	0.0	0.0	0.0	19.199736809639347	0.0
13	0.0	0.0	0.0	20.03536620471275	0.0
14	0.0	0.0	0.0	20.344614878480076	0.0
15	0.0	0.0	0.0	20.987786322325945	0.0
16	0.0	0.0	0.0	22.349796438705432	0.0
17	0.0	0.0	0.0	24.145248180285396	0.0
18	0.0	0.0	0.0	25.943167331496483	0.0
19	0.0	0.0	0.0	26.992638894600486	0.0
20	0.0	0.0	0.0	27.93765678332031	0.0
21	0.0	0.0	0.0	29.140930213430934	0.0
22	0.0	0.0	0.0	30.265246535345643	0.0
23	0.0	0.0	0.0	31.294978821400665	0.0
24	0.0	0.0	0.0	32.00970514454908	0.0
25	0.0	0.0	0.0	32.61504297405108	0.0
26	0.0	0.0	0.0	33.09947773162808	0.0
27	0.0	0.0	0.0	33.55512604350866	0.0
28	0.0	0.0	0.0	33.97129580129128	0.0
29	0.0	0.0	0.0	34.343052185713695	0.0
30	0.0	0.0	0.0	34.79623308796315	0.0
31	0.0	0.0	0.0	35.14002549656619	0.0
32	0.0	0.0	0.0	35.4813504955381	0.0
33	0.0	0.0	0.0	35.8062260969692	0.0
34	0.0	0.0	0.0	36.13110169840029	0.0
35	0.0	0.0	0.0	36.43212567339721	0.0
36	0.0	0.0	0.0	36.68791380515689	0.0
37	0.0	0.0	0.0	36.98235802113748	0.0
38	0.0	0.0	0.0	37.264465188962454	0.0
39	0.0	0.0	0.0	37.530945429123655	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGAG	75	0.0	45.000004	5
CTCGTCC	20	7.0083904E-4	45.0	37
CTCGTAC	20	7.0083904E-4	45.0	29
TTTACGG	20	7.0083904E-4	45.0	2
AGATAGG	25	3.870184E-5	45.0	2
TTAGCAA	20	7.0083904E-4	45.0	11
TATGGGT	20	7.0083904E-4	45.0	4
ATTACTT	20	7.0083904E-4	45.0	23
TAGAAGG	20	7.0083904E-4	45.0	2
TCCTCGT	20	7.0083904E-4	45.0	35
GTTAGAG	20	7.0083904E-4	45.0	1
AGGGTCC	25	3.870184E-5	45.0	6
AGGGTAC	20	7.0083904E-4	45.0	6
TTTCCGC	20	7.0083904E-4	45.0	33
CACGAAC	20	7.0083904E-4	45.0	18
CCTCGTC	20	7.0083904E-4	45.0	36
CCAGATT	20	7.0083904E-4	45.0	19
GACTTTA	35	1.1999873E-7	45.0	9
CCCAAGC	20	7.0083904E-4	45.0	39
CAGATTA	20	7.0083904E-4	45.0	20
>>END_MODULE