##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934797.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	637033
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17606466227024	33.0	31.0	34.0	30.0	34.0
2	32.34699458269823	34.0	31.0	34.0	30.0	34.0
3	32.34860360452284	34.0	31.0	34.0	30.0	34.0
4	35.915527139096405	37.0	35.0	37.0	35.0	37.0
5	35.805353882765885	37.0	35.0	37.0	35.0	37.0
6	35.72668134931785	37.0	35.0	37.0	33.0	37.0
7	36.02888233419619	37.0	35.0	37.0	35.0	37.0
8	35.85931184098783	37.0	35.0	37.0	35.0	37.0
9	37.58058530719759	39.0	38.0	39.0	35.0	39.0
10	37.36869361555838	39.0	37.0	39.0	34.0	39.0
11	37.193727797461044	39.0	37.0	39.0	34.0	39.0
12	36.957049320835814	39.0	35.0	39.0	33.0	39.0
13	36.73168422985936	39.0	35.0	39.0	33.0	39.0
14	37.788897906387895	40.0	36.0	41.0	33.0	41.0
15	37.929162225504804	40.0	36.0	41.0	33.0	41.0
16	38.066459665354856	40.0	36.0	41.0	34.0	41.0
17	38.079355386612626	40.0	36.0	41.0	34.0	41.0
18	38.065261925206386	40.0	36.0	41.0	34.0	41.0
19	38.022731946382684	40.0	36.0	41.0	34.0	41.0
20	37.93721832306961	40.0	35.0	41.0	34.0	41.0
21	37.87599543508735	40.0	35.0	41.0	34.0	41.0
22	37.790608963742855	39.0	35.0	41.0	34.0	41.0
23	37.639822426781656	39.0	35.0	41.0	33.0	41.0
24	37.413300409868874	39.0	35.0	41.0	33.0	41.0
25	37.31966161878584	39.0	35.0	41.0	33.0	41.0
26	37.42991493376324	39.0	35.0	41.0	33.0	41.0
27	37.500810790021866	39.0	35.0	41.0	33.0	41.0
28	37.48832164110807	39.0	35.0	41.0	33.0	41.0
29	37.47526266300176	39.0	35.0	41.0	33.0	41.0
30	37.278965139953506	39.0	35.0	41.0	33.0	41.0
31	37.204319085510484	39.0	35.0	41.0	33.0	41.0
32	37.03727122456765	39.0	35.0	41.0	32.0	41.0
33	36.84450099131442	39.0	35.0	41.0	32.0	41.0
34	36.86072778019349	39.0	35.0	41.0	32.0	41.0
35	36.745975483216725	39.0	35.0	41.0	32.0	41.0
36	36.60713495219243	39.0	35.0	41.0	31.0	41.0
37	36.58298706660408	39.0	35.0	41.0	31.0	41.0
38	36.509452414553095	39.0	35.0	41.0	31.0	41.0
39	36.517678048076	39.0	35.0	41.0	31.0	41.0
40	36.461696646798515	39.0	35.0	40.0	31.0	41.0
41	36.424806878136614	39.0	35.0	40.0	31.0	41.0
42	36.319110626922	39.0	35.0	40.0	31.0	41.0
43	36.24332334431654	38.0	35.0	40.0	31.0	41.0
44	36.161048171758765	38.0	35.0	40.0	30.0	41.0
45	36.12578155291798	38.0	35.0	40.0	30.0	41.0
46	36.06656484043998	38.0	35.0	40.0	30.0	41.0
47	35.93121235477597	38.0	35.0	40.0	30.0	41.0
48	35.922534625364776	38.0	35.0	40.0	30.0	41.0
49	35.92242945027966	38.0	35.0	40.0	30.0	41.0
50	35.81845681463911	38.0	35.0	40.0	30.0	41.0
51	34.863154656038226	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	12.0
10	8.0
11	14.0
12	12.0
13	6.0
14	15.0
15	22.0
16	34.0
17	68.0
18	133.0
19	284.0
20	475.0
21	748.0
22	1167.0
23	1789.0
24	2660.0
25	3878.0
26	5263.0
27	6167.0
28	6900.0
29	7772.0
30	9475.0
31	11929.0
32	16427.0
33	23345.0
34	46537.0
35	69101.0
36	48541.0
37	74289.0
38	131540.0
39	168298.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.805107427715676	21.009272675041952	24.459957333450543	13.725662563791829
2	33.24741418419454	24.21161855037337	24.13469945826982	18.406267807162266
3	31.04062112951762	23.95935532382153	27.654454321832624	17.34556922482823
4	28.10702114333166	25.29963125929112	27.16766635323445	19.425681244142766
5	24.24992111868616	27.951613181734697	27.166410531322555	20.63205516825659
6	25.15128729594856	33.35478695766154	25.394759769117144	16.099165977272765
7	76.40420511967197	6.5048435481364395	11.123599562346064	5.967351769845519
8	75.831550327848	4.704151904218463	11.821679567620516	7.642618200313013
9	71.18783485313948	6.488831818759781	14.44273687548369	7.880596452617055
10	41.66989779179415	22.752196511012773	21.13893628744508	14.438969409748005
11	30.00880645115716	23.838168509323694	25.485806857729504	20.66721818178964
12	28.394918316633515	20.339605640524116	31.32914621377542	19.93632982906694
13	22.472776135616208	27.977357530928536	31.740898823137893	17.808967510317363
14	17.59123938634262	31.10372618059033	30.13674330843143	21.168291124635616
15	15.79886756259095	24.85287261413459	39.57110542154017	19.77715440173429
16	17.711170378928564	23.915401556905216	36.434219263366266	21.93920880079996
17	17.351534378909726	24.937012682231533	28.618925550167727	29.092527388691007
18	18.906241905835333	25.404963322151286	34.40952038591407	21.279274386099306
19	21.72775978638469	26.084676931964278	29.0973936985996	23.090169583051427
20	23.462363802189213	25.78845993849612	29.708037103258388	21.04113915605628
21	21.243797417088285	26.578685876555845	31.24265147959368	20.93486522676219
22	19.352372640035917	25.06400767307188	29.283883252515963	26.299736434376243
23	18.639850682774675	26.227055741225335	30.50328632896569	24.6298072470343
24	21.715358545004733	23.23882750187196	31.755497752863665	23.290316200259642
25	18.036428254109286	26.4242197813928	29.97960859170561	25.559743372792305
26	17.013247351393098	31.62552018498257	28.632425635720598	22.728806827903735
27	20.371472121538446	30.267819720485438	28.395703205328456	20.965004952647664
28	18.038311986977128	26.873019137156163	31.9685165446688	23.120152331197914
29	19.84104434150193	25.439812380206362	31.052865393158598	23.66627788513311
30	20.054848022002002	31.034969930914098	27.647704279056185	21.262477768027715
31	24.244269920082633	28.950619512646913	23.095192870699005	23.709917696571452
32	25.880919826759367	29.809758678121856	23.090169583051427	21.21915191206735
33	23.348240985945782	31.025237311096916	23.89028511866732	21.73623658428998
34	21.19450640704642	25.965059894856314	26.887932022359912	25.952501675737366
35	21.58365422199478	28.363365791097166	25.546400264978423	24.506579721929633
36	24.05589663329843	30.400936843146276	25.316427877362713	20.226738646192583
37	23.35232240715944	27.603436556661897	28.416424266874714	20.627816769303944
38	23.423747278398448	28.90713667894756	24.588679079419748	23.080436963234245
39	22.525520655915784	28.052392890164246	27.240661001863327	22.181425452056644
40	23.277130070184747	24.975158272805334	27.562936300003297	24.18477535700662
41	19.879032954336743	26.88557735627511	25.974792514673496	27.26059717471465
42	23.0046167153036	26.565342768741967	25.94402487783207	24.486015638122357
43	24.372206777356904	26.00854272855566	27.30282418650211	22.31642630758532
44	22.09084929666124	26.052339517732992	27.20534101059129	24.65147017501448
45	22.22475130801701	25.878879116152536	24.969821029679782	26.92654854615067
46	23.915244579166227	26.51746455835098	26.70191340166051	22.86537746082228
47	20.10005761083021	25.18911893104439	29.582611889807907	25.1282115683175
48	21.54488072046503	23.957157635475713	28.8350838967526	25.662877747306656
49	22.317211196280258	21.94313324427463	31.996615559947443	23.743039999497668
50	21.324954908144473	23.217635507108735	30.058725372154978	25.39868421259181
51	19.344366775347588	24.04647796895922	26.477906168126296	30.131249087566893
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	574.0
1	720.0
2	866.0
3	3172.0
4	5478.0
5	3645.5
6	1813.0
7	1771.5
8	1730.0
9	1806.5
10	1883.0
11	1880.0
12	1877.0
13	1813.0
14	1749.0
15	1721.5
16	1694.0
17	1545.5
18	1397.0
19	1433.5
20	1470.0
21	1714.0
22	1958.0
23	1979.0
24	2000.0
25	2359.5
26	2988.5
27	3258.0
28	3823.0
29	4388.0
30	5611.0
31	6834.0
32	8184.0
33	9534.0
34	10819.5
35	12105.0
36	14501.5
37	16898.0
38	18494.0
39	20090.0
40	23759.5
41	27429.0
42	31988.5
43	36548.0
44	44358.5
45	52169.0
46	60378.0
47	68587.0
48	77769.0
49	86951.0
50	83458.0
51	79965.0
52	65376.5
53	50788.0
54	42241.5
55	33695.0
56	29534.5
57	25374.0
58	22996.5
59	20619.0
60	18740.0
61	16861.0
62	15045.5
63	13230.0
64	11208.0
65	9186.0
66	7376.0
67	5566.0
68	4448.5
69	3331.0
70	3311.5
71	3292.0
72	2323.0
73	1354.0
74	1155.5
75	606.0
76	255.0
77	275.5
78	296.0
79	248.0
80	200.0
81	108.0
82	16.0
83	21.0
84	26.0
85	20.5
86	15.0
87	10.5
88	6.0
89	3.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	637033.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.087041663439088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.06245271925619	15.912490510376987
2	12.485712227907376	6.014877412626317
3	6.128719585471387	4.428681719963533
4	3.424922499875265	3.2998500379418183
5	2.250247681441933	2.7100904827974523
6	1.5059877897090943	2.17648743812121
7	1.0832538779155803	1.8264666902604179
8	0.8219674745005704	1.5839011843429638
9	0.5989637113914862	1.298453748405735
>10	3.848592713837564	19.303718113064665
>50	1.3711778344513121	24.2457279007456
>100	0.407469553300702	13.154917541973003
>500	0.005924436154614407	0.9172576474738304
>1k	0.004607894786922316	3.127079571906462
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC	4567	0.7169173339528722	No Hit
CCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC	4517	0.7090684470035304	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3408	0.5349801344671312	No Hit
CTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGCT	2872	0.4508400663701881	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC	1686	0.2646644679318026	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTG	1392	0.2185130126696733	No Hit
ACTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC	1280	0.20093150590314787	No Hit
GCCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTG	742	0.11647748232823103	No Hit
ACCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTG	726	0.1139658385044417	No Hit
TGAGATGGGGGGAGGAAAGCAAGCCAGGTAAATCTGTCACTTTGGTCTGAA	681	0.10690184025003413	No Hit
AGCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTG	658	0.10329135225333695	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTC	639	0.1003087752125871	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4869449463371599	0.0
2	0.0	0.0	0.0	2.537388172983189	0.0
3	0.0	0.0	0.0	3.535766592939455	0.0
4	0.0	0.0	0.0	4.579511579462916	0.0
5	0.0	0.0	0.0	7.351895427709397	0.0
6	0.0	0.0	0.0	8.69154345222304	0.0
7	0.0	0.0	0.0	10.155203890536283	0.0
8	1.5697773898683428E-4	0.0	0.0	12.27518825555348	0.0
9	1.5697773898683428E-4	0.0	0.0	12.944698312332328	0.0
10	1.5697773898683428E-4	0.0	0.0	14.52201063367204	0.0
11	1.5697773898683428E-4	0.0	0.0	17.017799705823716	0.0
12	1.5697773898683428E-4	0.0	0.0	18.88018360116352	0.0
13	1.5697773898683428E-4	0.0	0.0	19.665386251575665	0.0
14	1.5697773898683428E-4	0.0	0.0	19.930992585941386	0.0
15	1.5697773898683428E-4	0.0	0.0	20.509769509585844	0.0
16	1.5697773898683428E-4	0.0	0.0	21.70609685840451	0.0
17	1.5697773898683428E-4	0.0	0.0	23.166146808721056	0.0
18	1.5697773898683428E-4	0.0	0.0	24.798558316445146	0.0
19	1.5697773898683428E-4	0.0	0.0	25.68689534137164	0.0
20	1.5697773898683428E-4	0.0	0.0	26.479161990038193	0.0
21	1.5697773898683428E-4	0.0	0.0	27.537662884026417	0.0
22	1.5697773898683428E-4	0.0	0.0	28.571047339776747	0.0
23	1.5697773898683428E-4	0.0	0.0	29.60615855065593	0.0
24	1.5697773898683428E-4	0.0	0.0	30.38571000246455	0.0
25	1.5697773898683428E-4	0.0	0.0	31.054906103765425	0.0
26	1.5697773898683428E-4	0.0	0.0	31.69835785587246	0.0
27	1.5697773898683428E-4	0.0	0.0	32.28718135481208	0.0
28	1.5697773898683428E-4	0.0	0.0	32.93691221647858	0.0
29	1.5697773898683428E-4	0.0	0.0	33.57753836928385	0.0
30	1.5697773898683428E-4	0.0	0.0	34.362741019695996	0.0
31	1.5697773898683428E-4	0.0	0.0	35.08373977486253	0.0
32	1.5697773898683428E-4	0.0	0.0	35.691086647002585	0.0
33	1.5697773898683428E-4	0.0	0.0	36.2656251716944	0.0
34	3.1395547797366855E-4	0.0	0.0	36.837338097084455	0.0
35	3.1395547797366855E-4	0.0	0.0	37.59334288804504	0.0
36	3.1395547797366855E-4	0.0	0.0	38.21340495704304	0.0
37	3.1395547797366855E-4	0.0	0.0	38.825932094569666	0.0
38	3.1395547797366855E-4	0.0	0.0	39.414284660292324	0.0
39	3.1395547797366855E-4	0.0	0.0	40.01299775678811	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	35	1.2107921E-7	45.000004	25
CTCACGA	35	1.2107921E-7	45.000004	24
ACACGCG	30	2.1639717E-6	45.000004	1
CGCTGTT	30	2.1639717E-6	45.000004	29
TAACGGA	30	2.1639717E-6	45.000004	29
ACGGGAA	125	0.0	45.000004	5
ATCGTGT	30	2.1639717E-6	45.000004	40
CGTTATC	30	2.1639717E-6	45.000004	42
CGCCTAG	35	1.2107921E-7	45.000004	11
GCCGATT	35	1.2107921E-7	45.000004	9
TTGACGG	30	2.1639717E-6	45.000004	2
CGCACGT	35	1.2107921E-7	45.000004	15
CGTCCGC	30	2.1639717E-6	45.000004	29
TCGACCG	35	1.2107921E-7	45.000004	1
ATACCGG	35	1.2107921E-7	45.000004	2
AATCGTG	30	2.1639717E-6	45.000004	39
TCTGCGC	20	7.030917E-4	45.0	12
CTTCGTT	20	7.030917E-4	45.0	25
TATCAGC	20	7.030917E-4	45.0	19
TGATATC	20	7.030917E-4	45.0	45
>>END_MODULE