FastQCFastQC Report
Sat 14 Jan 2017
SRR2934796.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934796.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565438
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC69491.2289587894694025RNA PCR Primer, Index 30 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC52270.9244161163558162Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT41560.7350054294193175RNA PCR Primer, Index 30 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34810.6156289460559778No Hit
TCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC21320.37705283337872586Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG19040.3367301101093312RNA PCR Primer, Index 30 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC16900.29888334353191687Illumina Single End Adapter 2 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG11780.20833407022520595Illumina Single End Adapter 2 (95% over 21bp)
AAAACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC10060.17791517372373275RNA PCR Primer, Index 30 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG9660.17084101174664595Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8950.1582843742373169No Hit
CGTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC8230.1455508826785607No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCC7940.14042211524517276No Hit
AGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG7840.13865357475090107RNA PCR Primer, Index 30 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG7260.12839603988412523RNA PCR Primer, Index 30 (95% over 23bp)
ATAAGCGGGGAAGGAATGTTTTTTGTTTGTTTGTTTTGTCTCTTGATGTTT6500.11495513212766034No Hit
AGCCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT5940.1050513053597388RNA PCR Primer, Index 30 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG351.2104647E-745.0000041
TCACATA302.163537E-645.00000432
GACCGAC302.163537E-645.0000049
TTCGTAG351.2104647E-745.0000041
AAGCGGT302.163537E-645.00000415
CGCGAGG351.2104647E-745.0000042
CCGTCCG351.2104647E-745.00000413
GTGGACG302.163537E-645.0000041
AAACGTA302.163537E-645.00000431
AAGTTCG351.2104647E-745.0000041
TGATTAG351.2104647E-745.0000041
TACGAAG302.163537E-645.0000041
ACATGCG302.163537E-645.0000041
ACGTAAA302.163537E-645.00000433
GCCAGCG302.163537E-645.0000041
GGCGAGT302.163537E-645.00000424
CATCCGC302.163537E-645.00000414
GCTCGGA302.163537E-645.00000413
CGATCTT302.163537E-645.00000410
AGGGCGC302.163537E-645.0000046
Produced by FastQC (version 0.11.5)