##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934796.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	565438
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99006787658417	33.0	31.0	34.0	30.0	34.0
2	32.17912662396231	33.0	31.0	34.0	30.0	34.0
3	32.17707157990797	34.0	31.0	34.0	30.0	34.0
4	35.78467842628193	37.0	35.0	37.0	35.0	37.0
5	35.665830736526374	37.0	35.0	37.0	33.0	37.0
6	35.61348016935544	37.0	35.0	37.0	33.0	37.0
7	35.973206611511785	37.0	35.0	37.0	35.0	37.0
8	35.79924589433324	37.0	35.0	37.0	35.0	37.0
9	37.46780725738277	39.0	37.0	39.0	35.0	39.0
10	37.25873040014997	39.0	37.0	39.0	34.0	39.0
11	37.10921444968326	39.0	37.0	39.0	34.0	39.0
12	36.855117625628274	39.0	35.0	39.0	33.0	39.0
13	36.56019934988451	39.0	35.0	39.0	33.0	39.0
14	37.53230416066837	39.0	36.0	41.0	33.0	41.0
15	37.69400535514062	39.0	36.0	41.0	33.0	41.0
16	37.85476568606991	39.0	36.0	41.0	33.0	41.0
17	37.9021908679643	39.0	36.0	41.0	34.0	41.0
18	37.88788868098713	39.0	36.0	41.0	34.0	41.0
19	37.84984030079337	39.0	36.0	41.0	34.0	41.0
20	37.76311814911626	39.0	35.0	41.0	34.0	41.0
21	37.66848708434877	39.0	35.0	41.0	33.0	41.0
22	37.584074646557184	39.0	35.0	41.0	33.0	41.0
23	37.42105412087621	39.0	35.0	41.0	33.0	41.0
24	37.136018449414436	39.0	35.0	41.0	32.0	41.0
25	37.042183227869366	39.0	35.0	40.0	32.0	41.0
26	37.1865385771738	39.0	35.0	40.0	33.0	41.0
27	37.30856256565706	39.0	35.0	40.0	33.0	41.0
28	37.32269850982778	39.0	35.0	41.0	33.0	41.0
29	37.30951934606447	39.0	35.0	41.0	33.0	41.0
30	37.15185572954064	39.0	35.0	41.0	33.0	41.0
31	36.88245749312922	39.0	35.0	40.0	32.0	41.0
32	36.760005517846345	39.0	35.0	40.0	31.0	41.0
33	36.64076698064156	39.0	35.0	40.0	31.0	41.0
34	36.507834634389624	39.0	35.0	40.0	31.0	41.0
35	36.433465738065	39.0	35.0	40.0	31.0	41.0
36	36.401271226907284	39.0	35.0	40.0	31.0	41.0
37	36.284395459802845	38.0	35.0	40.0	30.0	41.0
38	36.263972354174996	38.0	35.0	40.0	30.0	41.0
39	36.309430919039755	39.0	35.0	40.0	30.0	41.0
40	36.08714483285524	38.0	35.0	40.0	30.0	41.0
41	36.05090213250613	38.0	35.0	40.0	30.0	41.0
42	36.0518376904276	38.0	35.0	40.0	30.0	41.0
43	36.04155185891291	38.0	35.0	40.0	30.0	41.0
44	35.96532776360981	38.0	35.0	40.0	30.0	41.0
45	35.89820104060923	38.0	35.0	40.0	30.0	41.0
46	35.78424690240132	38.0	35.0	40.0	29.0	41.0
47	35.58940325906642	38.0	35.0	40.0	28.0	41.0
48	35.63567181547756	38.0	35.0	40.0	28.0	41.0
49	35.682120763019114	38.0	35.0	40.0	29.0	41.0
50	35.54067112574677	38.0	35.0	40.0	28.0	41.0
51	34.60139926923907	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	5.0
10	9.0
11	15.0
12	4.0
13	15.0
14	14.0
15	21.0
16	31.0
17	83.0
18	154.0
19	307.0
20	469.0
21	830.0
22	1281.0
23	1869.0
24	2667.0
25	4016.0
26	5231.0
27	6047.0
28	6705.0
29	7725.0
30	9161.0
31	11708.0
32	15877.0
33	22954.0
34	43259.0
35	60097.0
36	45433.0
37	69471.0
38	122004.0
39	127915.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.869050187642145	23.204842971289512	23.930121427990336	14.995985413078003
2	31.7175357864169	24.358285081653513	25.214966097078722	18.70921303485086
3	30.119659449842423	24.441229630834858	28.80757925714225	16.631531662180468
4	28.341038274753377	25.77612399591113	27.806231629285616	18.076606100049872
5	23.275761445109808	28.272595757625062	28.12775229114421	20.323890506120918
6	23.470831461627977	30.83468037167647	27.954435322705585	17.74005284398997
7	73.45120066214156	5.842196668777125	13.591410552527423	7.115192116553892
8	71.32258532323614	4.962524626926382	14.33578924656638	9.379100803271093
9	65.95400379882498	6.765197952737524	17.415171955192257	9.865626293245237
10	38.525709273165226	23.61302211736742	23.23455445159328	14.626714157874074
11	29.917692125396595	23.982293372571352	27.058139000208687	19.041875501823366
12	29.069500104343888	20.7403817925219	32.089813560461096	18.10030454267311
13	21.57902369490554	28.696161206003133	32.72684184649776	16.997973252593564
14	15.80279358656475	32.42159175718646	30.389538729268285	21.3860759269805
15	13.111782370480936	26.6129973578005	41.54902924812269	18.726191023595867
16	14.876962637813518	26.18872449322472	36.93384597427127	22.000466894690486
17	14.563400408179147	25.695655403421775	29.949525854293483	29.791418334105597
18	17.53012708731992	26.022304832713754	34.49520548672003	21.952362593246296
19	19.598788903469522	26.71628012266597	30.99084249732066	22.694088476543847
20	22.494950816888853	27.545902468528823	30.20207343687548	19.75707327770684
21	19.160367714939568	27.783771165008364	32.26932042062967	20.786540699422396
22	16.26296782317425	26.61052140110852	28.852323331647323	28.27418744406991
23	17.196757204149705	26.47487434519788	30.16104329740838	26.167325153244036
24	21.661260827889176	23.40062040400539	31.40273557843654	23.53538318966889
25	16.093187935724167	27.39009405098349	29.781337653288247	26.7353803600041
26	15.861332276925143	31.989183606337036	29.298879806450927	22.850604310286894
27	18.61777949129701	29.4028699875141	31.26761908467418	20.711731436514704
28	14.393620520729064	26.622193768370717	32.71499262518614	26.269193085714083
29	18.386277540596847	25.973493115071854	31.536614093852904	24.10361525047839
30	22.45268269907576	26.121519954442395	29.66991960214913	21.75587774433271
31	20.49313983142272	31.405388389177947	24.132796168633874	23.968675610765462
32	23.441827397521923	31.82400192417206	23.00800441427707	21.726166264028947
33	23.65670506757593	27.151517938306235	24.84410315542995	24.347673838687882
34	18.596910713464606	27.82285590993177	26.843968746352388	26.736264630251238
35	19.030910550758882	28.748686858683005	25.799291876386093	26.421110714172023
36	21.75039526880047	26.730959008768423	27.072641032261718	24.44600469016939
37	18.961937471482283	27.656082541321947	30.576827167611658	22.80515281958411
38	20.50251309604236	28.64275128307613	24.623743009843697	26.230992611037813
39	24.600221421269882	23.76883053491276	27.881394600292165	23.749553443525194
40	23.92817603344664	25.921851732639123	28.664327477106244	21.485644756807996
41	16.585549609329405	28.40665112709086	26.37194528843127	28.635853975148468
42	19.683855701243992	24.4935784294653	27.511062220791672	28.31150364849904
43	23.387179496248926	21.915223242866592	29.109822827613286	25.5877744332712
44	20.46183666467411	24.27604794866988	26.889420237055166	28.37269514960084
45	22.242403234306856	24.093711423710467	25.6040450058185	28.05984033616418
46	24.195756210229945	24.43857682009345	28.17567973853897	23.189987231137632
47	17.126546146527115	25.977030196060397	31.932236602421487	24.964187054991
48	19.00721210813564	22.250892228679362	30.285902256303963	28.455993406881035
49	21.807872834864302	18.83088862085675	33.55328081947092	25.807957724808023
50	20.401706287868873	20.98426352668197	31.991482708979586	26.62254747646957
51	18.492389970253146	21.743674814922237	26.1970366335478	33.56689858127682
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1055.0
1	1133.0
2	1211.0
3	3316.0
4	5421.0
5	3572.0
6	1723.0
7	1768.5
8	1814.0
9	1870.5
10	1927.0
11	1977.0
12	2027.0
13	2004.0
14	1981.0
15	1870.5
16	1760.0
17	1774.5
18	1789.0
19	1896.5
20	2004.0
21	1921.5
22	1839.0
23	2034.0
24	2229.0
25	2425.0
26	2876.5
27	3132.0
28	3631.0
29	4130.0
30	4926.0
31	5722.0
32	6968.5
33	8215.0
34	8965.5
35	9716.0
36	10714.5
37	11713.0
38	13297.0
39	14881.0
40	18401.5
41	21922.0
42	27715.5
43	33509.0
44	40990.5
45	48472.0
46	58090.0
47	67708.0
48	78393.0
49	89078.0
50	85246.5
51	81415.0
52	63150.5
53	44886.0
54	36052.5
55	27219.0
56	22251.0
57	17283.0
58	15187.0
59	13091.0
60	12671.5
61	12252.0
62	10579.0
63	8906.0
64	7074.5
65	5243.0
66	3900.0
67	2557.0
68	2318.5
69	2080.0
70	1707.5
71	1335.0
72	1056.0
73	777.0
74	647.0
75	297.0
76	77.0
77	99.5
78	122.0
79	77.0
80	32.0
81	19.5
82	7.0
83	7.0
84	7.0
85	4.5
86	2.0
87	1.5
88	1.0
89	14.0
90	27.0
91	14.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	565438.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.31331689630751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.19473349679366	16.502949489746523
2	12.628081007941194	6.393172326925156
3	6.189326568895062	4.700171544395291
4	3.641475786072195	3.6871132217230387
5	2.297125632849457	2.9073934547474622
6	1.602672495724107	2.4341374059156218
7	1.1510819422644023	2.0396391384709176
8	0.8682945698882101	1.7583532485538633
9	0.6443855836655579	1.468038283446451
>10	4.453608387953213	23.525586933004966
>50	1.0980467839611752	19.498212273695938
>100	0.21707214984598325	8.753527521626864
>500	0.007752576780213688	1.3858354418130168
>1k	0.004933457951045074	2.7736333179540384
>5k	0.0014095594145843069	2.172236397980856
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	6949	1.2289587894694025	RNA PCR Primer, Index 30 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	5227	0.9244161163558162	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	4156	0.7350054294193175	RNA PCR Primer, Index 30 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3481	0.6156289460559778	No Hit
TCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	2132	0.37705283337872586	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG	1904	0.3367301101093312	RNA PCR Primer, Index 30 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	1690	0.29888334353191687	Illumina Single End Adapter 2 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG	1178	0.20833407022520595	Illumina Single End Adapter 2 (95% over 21bp)
AAAACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	1006	0.17791517372373275	RNA PCR Primer, Index 30 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG	966	0.17084101174664595	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	895	0.1582843742373169	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	823	0.1455508826785607	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCC	794	0.14042211524517276	No Hit
AGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG	784	0.13865357475090107	RNA PCR Primer, Index 30 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG	726	0.12839603988412523	RNA PCR Primer, Index 30 (95% over 23bp)
ATAAGCGGGGAAGGAATGTTTTTTGTTTGTTTGTTTTGTCTCTTGATGTTT	650	0.11495513212766034	No Hit
AGCCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT	594	0.1050513053597388	RNA PCR Primer, Index 30 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7850551254072065	0.0
2	0.0	0.0	0.0	3.825353089109681	0.0
3	0.0	0.0	0.0	5.282984164488414	0.0
4	0.0	0.0	0.0	6.768204471577786	0.0
5	0.0	0.0	0.0	10.573042490953915	0.0
6	0.0	0.0	0.0	12.470155879159165	0.0
7	0.0	0.0	0.0	14.479748442800094	0.0
8	0.0	0.0	0.0	17.293673223235793	0.0
9	0.0	0.0	0.0	18.26672420318408	0.0
10	0.0	0.0	0.0	20.3100958902656	0.0
11	0.0	0.0	0.0	23.687123964077404	0.0
12	0.0	0.0	0.0	26.074830485393623	0.0
13	0.0	0.0	0.0	27.132417700968098	0.0
14	0.0	0.0	0.0	27.49408423204666	0.0
15	0.0	0.0	0.0	28.291872849012623	0.0
16	0.0	0.0	0.0	29.789119231463044	0.0
17	0.0	0.0	0.0	31.653691474573694	0.0
18	0.0	0.0	0.0	33.84119921193835	0.0
19	0.0	0.0	0.0	34.89878642751283	0.0
20	1.7685404942716974E-4	0.0	0.0	35.938334530045736	0.0
21	1.7685404942716974E-4	0.0	0.0	37.328761066642144	0.0
22	3.537080988543395E-4	0.0	0.0	38.56249491544608	0.0
23	3.537080988543395E-4	0.0	0.0	39.863787011131194	0.0
24	3.537080988543395E-4	0.0	0.0	40.75672310668897	0.0
25	3.537080988543395E-4	0.0	0.0	41.57644162578391	0.0
26	3.537080988543395E-4	0.0	0.0	42.276783661515495	0.0
27	3.537080988543395E-4	0.0	0.0	42.96669130833089	0.0
28	3.537080988543395E-4	0.0	0.0	43.62582635054595	0.0
29	3.537080988543395E-4	0.0	0.0	44.32298501338785	0.0
30	3.537080988543395E-4	0.0	0.0	45.21804335753876	0.0
31	3.537080988543395E-4	0.0	0.0	45.9877121806458	0.0
32	3.537080988543395E-4	0.0	0.0	46.714051761643184	0.0
33	3.537080988543395E-4	0.0	0.0	47.36080702039835	0.0
34	3.537080988543395E-4	0.0	0.0	48.01622812757544	0.0
35	5.305621482815092E-4	0.0	0.0	48.76538188094893	0.0
36	5.305621482815092E-4	0.0	0.0	49.476512013695576	0.0
37	5.305621482815092E-4	0.0	0.0	50.11442456997938	0.0
38	5.305621482815092E-4	0.0	0.0	50.70670878151097	0.0
39	5.305621482815092E-4	0.0	0.0	51.34886583498103	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCG	35	1.2104647E-7	45.000004	1
TCACATA	30	2.163537E-6	45.000004	32
GACCGAC	30	2.163537E-6	45.000004	9
TTCGTAG	35	1.2104647E-7	45.000004	1
AAGCGGT	30	2.163537E-6	45.000004	15
CGCGAGG	35	1.2104647E-7	45.000004	2
CCGTCCG	35	1.2104647E-7	45.000004	13
GTGGACG	30	2.163537E-6	45.000004	1
AAACGTA	30	2.163537E-6	45.000004	31
AAGTTCG	35	1.2104647E-7	45.000004	1
TGATTAG	35	1.2104647E-7	45.000004	1
TACGAAG	30	2.163537E-6	45.000004	1
ACATGCG	30	2.163537E-6	45.000004	1
ACGTAAA	30	2.163537E-6	45.000004	33
GCCAGCG	30	2.163537E-6	45.000004	1
GGCGAGT	30	2.163537E-6	45.000004	24
CATCCGC	30	2.163537E-6	45.000004	14
GCTCGGA	30	2.163537E-6	45.000004	13
CGATCTT	30	2.163537E-6	45.000004	10
AGGGCGC	30	2.163537E-6	45.000004	6
>>END_MODULE