##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934795.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1222061
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0411035128361	33.0	31.0	34.0	30.0	34.0
2	32.22156504462543	34.0	31.0	34.0	30.0	34.0
3	32.234854070296	34.0	31.0	34.0	30.0	34.0
4	35.82364055476773	37.0	35.0	37.0	35.0	37.0
5	35.69566576463859	37.0	35.0	37.0	33.0	37.0
6	35.61087130675146	37.0	35.0	37.0	33.0	37.0
7	35.97887012186789	37.0	35.0	37.0	35.0	37.0
8	35.82647101904078	37.0	35.0	37.0	35.0	37.0
9	37.55999495933509	39.0	37.0	39.0	35.0	39.0
10	37.282999784789794	39.0	37.0	39.0	34.0	39.0
11	37.084612797560844	39.0	37.0	39.0	33.0	39.0
12	36.71914004292748	39.0	35.0	39.0	33.0	39.0
13	36.41155228748811	39.0	35.0	39.0	32.0	39.0
14	36.891554513236244	39.0	35.0	41.0	30.0	41.0
15	37.16193708824682	39.0	35.0	41.0	31.0	41.0
16	37.49551290811179	39.0	35.0	41.0	33.0	41.0
17	37.60292734978041	39.0	35.0	41.0	33.0	41.0
18	37.623400141236814	39.0	35.0	41.0	33.0	41.0
19	37.59189598555228	39.0	35.0	41.0	33.0	41.0
20	37.44058111665457	39.0	35.0	41.0	33.0	41.0
21	37.40461155376041	39.0	35.0	41.0	33.0	41.0
22	37.44903077669609	39.0	35.0	41.0	33.0	41.0
23	37.3097324928952	39.0	35.0	41.0	33.0	41.0
24	36.9944225370092	39.0	35.0	41.0	32.0	41.0
25	36.91006668243238	39.0	35.0	40.0	32.0	41.0
26	37.09864728520098	39.0	35.0	41.0	33.0	41.0
27	37.137451403816996	39.0	35.0	41.0	33.0	41.0
28	37.07793391655572	39.0	35.0	41.0	33.0	41.0
29	37.057529861438994	39.0	35.0	41.0	32.0	41.0
30	36.885540083514655	39.0	35.0	41.0	32.0	41.0
31	36.70485761349065	39.0	35.0	41.0	31.0	41.0
32	36.64560443382123	38.0	35.0	40.0	31.0	41.0
33	36.53295621086018	38.0	35.0	40.0	31.0	41.0
34	36.38969331318158	38.0	35.0	40.0	31.0	41.0
35	36.32199292833991	38.0	35.0	40.0	31.0	41.0
36	36.238960248301844	38.0	35.0	40.0	30.0	41.0
37	36.0617947876579	38.0	35.0	40.0	30.0	41.0
38	36.02173377597354	38.0	35.0	40.0	30.0	41.0
39	36.09513436726972	38.0	35.0	40.0	30.0	41.0
40	35.974527458121976	38.0	35.0	40.0	30.0	41.0
41	35.85735409279897	38.0	35.0	40.0	30.0	41.0
42	35.81003648754031	38.0	35.0	40.0	30.0	41.0
43	35.756117738803546	38.0	35.0	40.0	30.0	41.0
44	35.70896051833746	38.0	35.0	40.0	30.0	41.0
45	35.73584542833787	38.0	35.0	40.0	30.0	41.0
46	35.736934572005815	38.0	35.0	40.0	30.0	41.0
47	35.587042708997345	38.0	35.0	40.0	29.0	41.0
48	35.517398067690564	37.0	34.0	40.0	29.0	41.0
49	35.53913675340265	37.0	34.0	40.0	29.0	41.0
50	35.39322750664656	37.0	34.0	40.0	29.0	41.0
51	34.44146732446253	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	14.0
10	14.0
11	20.0
12	25.0
13	24.0
14	32.0
15	75.0
16	126.0
17	265.0
18	437.0
19	728.0
20	1231.0
21	1973.0
22	2858.0
23	4354.0
24	6098.0
25	8489.0
26	11283.0
27	13431.0
28	15367.0
29	18360.0
30	22622.0
31	29597.0
32	40279.0
33	61535.0
34	111912.0
35	107305.0
36	95054.0
37	146424.0
38	244699.0
39	277214.0
40	208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.32125974071671	21.461940115918924	25.052595574198016	12.164204569166351
2	32.91136858143742	23.94225820151367	24.70056732028925	18.445805896759655
3	30.79797162334777	24.038980050913988	27.753606407536118	17.40944191820212
4	29.011235936667646	24.544437634455235	27.843372793993098	18.60095363488402
5	24.23954287060957	28.49857740325565	27.63773657779767	19.62414314833711
6	26.560949085192963	30.623512246933664	27.218690392705437	15.596848275167932
7	77.74161846258085	5.493260974697662	10.930387271993787	5.834733290727713
8	77.30514270564235	4.522196518831711	10.987503897104972	7.185156878420964
9	72.60226780823544	5.704543390223565	13.602103331994067	8.091085469546938
10	44.924353203318	20.611409741412253	20.333436710606097	14.130800344663646
11	34.21146734901122	21.755706139055253	24.453607471312807	19.57921904062072
12	31.09893859635485	18.51323297282214	31.68467040515981	18.703158025663203
13	27.562208433130586	21.836962312028614	33.22231868949259	17.37851056534821
14	19.15567226185927	25.558134986715064	35.255605080270136	20.03058767115553
15	17.462303436571496	22.76220254144433	40.75753992640302	19.017954095581153
16	24.750073850650665	23.05572307765324	30.93822648787581	21.255976583820285
17	24.579296778147736	22.686428909849834	27.2959369458644	25.438337366138025
18	25.497908860523328	23.110139346562896	31.645801641652916	19.74615015126086
19	24.918232395927863	24.969620992732768	27.97421732630368	22.13792928503569
20	21.57142728554467	29.917573672672642	29.570046012433092	18.9409530293496
21	21.338460191430705	25.16388298129144	34.53542826421921	18.962228563058638
22	18.795297452418495	22.3493753585132	32.74378283899085	26.111544350077452
23	17.89444225779237	25.030747237658353	33.83014432176463	23.244666182784655
24	22.071075011803828	25.699208141001144	29.28372642609493	22.945990421100092
25	22.513524283976004	25.123541296220075	26.697685303761432	25.66524911604249
26	17.44618312833811	25.646428451607573	27.57988349190425	29.327504928150066
27	19.631507756159472	26.179298742043155	30.448480067688926	23.740713434108446
28	16.041997903541642	26.734017369018403	29.36130029515712	27.86268443228284
29	18.98080374056614	28.041071599535538	27.587411757678215	25.390712902220102
30	24.831493681575633	26.40948365097978	26.80389931435501	21.955123353089576
31	22.278347807515335	27.141362010570667	26.67501867746373	23.90527150445027
32	25.170920273210584	27.123686951797005	24.36711424388799	23.33827853110442
33	26.867971402409534	26.370205742593868	24.26294595768951	22.498876897307092
34	19.216798506784848	25.2184629081527	29.24960374318467	26.31513484187778
35	19.57095431406452	27.95891530782833	26.952009760560237	25.518120617546913
36	27.695098689836268	26.227741495719116	23.974335160028836	22.10282465441578
37	19.847945397161027	30.260518910267166	27.266805830478187	22.624729862093627
38	23.075607518773612	28.139430028451933	26.130119527584956	22.654842925189495
39	24.159923277152288	23.397440880610706	25.644791872091492	26.797843970145518
40	24.95448263220903	20.84904108714704	29.112049234858162	25.084427045785766
41	21.058523265205256	21.909053639711928	26.797189338339084	30.235233756743728
42	21.81552312036797	21.69294331461359	31.30465664152608	25.186876923492363
43	22.988132343639148	23.137633882433036	29.40532428413966	24.468909489788153
44	22.504032122782743	23.904698701619644	26.980077099261003	26.611192076336614
45	20.63514014439541	22.612782831626244	25.623189022479238	31.128888001499107
46	23.287135421226925	22.477519534622246	27.91620058245865	26.319144461692172
47	18.13526493358351	23.575664389911797	32.922251835219356	25.366818841285337
48	19.819959887436063	25.539232493304343	29.013199832086944	25.62760778717265
49	24.381925288508512	20.660425297918845	30.330073539700553	24.62757587387209
50	20.761811398939987	22.18080766835698	29.13725255940579	27.920128373297242
51	19.617351343345383	22.54339186014446	25.343415754205395	32.49584104230476
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	834.0
1	1258.5
2	1683.0
3	5355.0
4	9027.0
5	5921.5
6	2816.0
7	2857.0
8	2898.0
9	2955.0
10	3012.0
11	2989.5
12	2967.0
13	3103.5
14	3240.0
15	3193.5
16	3147.0
17	2855.5
18	2564.0
19	2670.0
20	2776.0
21	2917.0
22	3058.0
23	3236.0
24	3414.0
25	3993.5
26	5579.5
27	6586.0
28	7466.0
29	8346.0
30	9501.5
31	10657.0
32	12227.5
33	13798.0
34	17767.5
35	21737.0
36	23060.5
37	24384.0
38	27374.5
39	30365.0
40	36196.5
41	42028.0
42	49904.5
43	57781.0
44	69089.0
45	80397.0
46	98854.5
47	117312.0
48	138309.0
49	159306.0
50	157721.5
51	156137.0
52	130595.0
53	105053.0
54	91208.5
55	77364.0
56	72496.0
57	67628.0
58	63411.0
59	59194.0
60	48944.0
61	38694.0
62	34020.5
63	29347.0
64	26164.0
65	22981.0
66	19165.0
67	15349.0
68	13314.0
69	11279.0
70	9656.0
71	8033.0
72	6335.5
73	4638.0
74	4306.5
75	2789.0
76	1603.0
77	1245.5
78	888.0
79	846.5
80	805.0
81	457.5
82	110.0
83	124.5
84	139.0
85	113.5
86	88.0
87	61.5
88	35.0
89	22.0
90	9.0
91	6.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1222061.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.695927398249726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.2308871009487	16.603291067649533
2	12.348302620948393	6.099055700371167
3	5.6086581874647035	4.155330461976817
4	3.178379152738654	3.1397208320057715
5	1.9923711036505907	2.460172606306283
6	1.375445463328416	2.038074078156631
7	1.0280009984226492	1.7771206615661783
8	0.7670958065362844	1.5155313876577519
9	0.5921169228017763	1.3160588883108937
>10	4.319520048283021	21.62699643819001
>50	1.1925433682994444	21.483777137016137
>100	0.35365510104338954	12.441310716350747
>500	0.007346942608939959	1.2484691265897814
>1k	0.004675327114779974	2.4037442967093257
>5k	0.0010018558103099943	1.6913466011430331
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	8336	0.6821263423020618	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	6101	0.4992385813801439	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6071	0.4967837121060242	No Hit
CTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGCT	4504	0.3685577070211716	TruSeq Adapter, Index 13 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	3851	0.31512338582116606	No Hit
TCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	3073	0.2514604426456617	TruSeq Adapter, Index 16 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2279	0.18648823585729354	No Hit
ACTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	2257	0.18468799838960576	TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTG	2078	0.17004061172069151	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGC	1620	0.13256294080246403	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTC	1582	0.12945343972191242	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCG	1545	0.12642576761716479	No Hit
ACCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTG	1543	0.1262621096655568	No Hit
GCCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTG	1392	0.11390593431915429	No Hit
AGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTG	1281	0.10482291800491138	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.454869274119704E-4	0.0	0.0	0.39400651849621254	0.0
2	2.454869274119704E-4	0.0	0.0	2.118715841516913	0.0
3	2.454869274119704E-4	0.0	0.0	3.0335637910055224	0.0
4	2.454869274119704E-4	0.0	0.0	4.028113162927219	0.0
5	2.454869274119704E-4	0.0	0.0	6.671352739347708	0.0
6	2.454869274119704E-4	0.0	0.0	7.914171223858711	0.0
7	2.454869274119704E-4	0.0	0.0	9.367372005161771	0.0
8	2.454869274119704E-4	0.0	0.0	11.507690696290938	0.0
9	2.454869274119704E-4	0.0	0.0	12.245624400091321	0.0
10	2.454869274119704E-4	0.0	0.0	14.004292748070677	0.0
11	2.454869274119704E-4	0.0	0.0	16.50858672357599	0.0
12	2.454869274119704E-4	0.0	0.0	18.537945323514947	0.0
13	2.454869274119704E-4	0.0	0.0	19.290117269105224	0.0
14	2.454869274119704E-4	0.0	0.0	19.605322483902196	0.0
15	2.454869274119704E-4	0.0	0.0	20.163968901716036	0.0
16	2.454869274119704E-4	0.0	0.0	21.25900425592503	0.0
17	2.454869274119704E-4	0.0	0.0	22.527271551911074	0.0
18	2.454869274119704E-4	0.0	0.0	24.084558790436812	0.0
19	2.454869274119704E-4	0.0	0.0	24.97600365284548	0.0
20	2.454869274119704E-4	0.0	0.0	25.900343763527353	0.0
21	2.454869274119704E-4	0.0	0.0	26.97263066246284	0.0
22	2.454869274119704E-4	0.0	0.0	28.074866966542587	0.0
23	2.454869274119704E-4	0.0	0.0	29.06172441473871	0.0
24	2.454869274119704E-4	0.0	0.0	29.81561476882087	0.0
25	2.454869274119704E-4	0.0	0.0	30.460508927132114	0.0
26	2.454869274119704E-4	0.0	0.0	31.10393016387889	0.0
27	2.454869274119704E-4	0.0	0.0	31.976554361852642	0.0
28	2.454869274119704E-4	0.0	0.0	32.69779495458901	0.0
29	2.454869274119704E-4	0.0	0.0	33.47713412014621	0.0
30	2.454869274119704E-4	0.0	0.0	34.41227565563421	0.0
31	2.454869274119704E-4	0.0	0.0	35.106267199427855	0.0
32	2.454869274119704E-4	0.0	0.0	35.70280043303894	0.0
33	2.454869274119704E-4	0.0	0.0	36.36471501831742	0.0
34	2.454869274119704E-4	0.0	0.0	37.10543090729514	0.0
35	2.454869274119704E-4	0.0	0.0	37.87912387352186	0.0
36	2.454869274119704E-4	0.0	0.0	38.48924071711641	0.0
37	2.454869274119704E-4	0.0	0.0	39.19002406590178	0.0
38	2.454869274119704E-4	0.0	0.0	40.079259545963744	0.0
39	2.454869274119704E-4	0.0	0.0	41.72696780275289	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGACG	35	1.2120108E-7	45.000004	1
TAGCGAT	35	1.2120108E-7	45.000004	12
TCCGTTG	35	1.2120108E-7	45.000004	16
GACGATT	35	1.2120108E-7	45.000004	18
ATGCACG	35	1.2120108E-7	45.000004	1
CTAGACG	25	3.8909515E-5	45.0	1
ACACGTA	25	3.8909515E-5	45.0	40
TCCGCGA	25	3.8909515E-5	45.0	45
CGAAATC	20	7.0334616E-4	45.0	34
ACCGACA	25	3.8909515E-5	45.0	12
ACCGAAC	25	3.8909515E-5	45.0	11
CGACATA	20	7.0334616E-4	45.0	33
ACCTCGT	25	3.8909515E-5	45.0	37
CTGCGAA	25	3.8909515E-5	45.0	35
CATACCG	20	7.0334616E-4	45.0	29
CGTAATT	25	3.8909515E-5	45.0	36
CGGTCTA	80	0.0	45.0	31
ACCCGTG	20	7.0334616E-4	45.0	20
CGGTATC	20	7.0334616E-4	45.0	45
CGCAATA	25	3.8909515E-5	45.0	9
>>END_MODULE