SRR2934794.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934794.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	907748
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	9706	1.0692394805606842	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	7988	0.8799799063176125	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	6118	0.6739755967515214	TruSeq Adapter, Index 16 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4023	0.4431846723980664	No Hit
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	3928	0.4327192128211794	TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	2965	0.326632501531262	TruSeq Adapter, Index 16 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	2266	0.24962875159185147	TruSeq Adapter, Index 16 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	1525	0.16799816689213307	TruSeq Adapter, Index 16 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	1506	0.16590507497675566	No Hit
ACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	1498	0.16502377311764937	TruSeq Adapter, Index 16 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	1443	0.15896482283629376	No Hit
GGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	1363	0.15015180424523106	No Hit
AAAACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	1222	0.13461885897848302	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	1114	0.12272128388054834	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	1107	0.12195014475383036	No Hit
GGTTGGAGGGAAATGACAGAAATACTGAAGAAAAAATAAGTCAAAATCATA	1089	0.11996721557084125	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCC	1054	0.11611151993725131	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	1016	0.11192533610649652	No Hit
GGCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	939	0.10344280571259865	No Hit
AGCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	926	0.10201069019155096	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	919	0.10123955106483297	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCG	30	2.1649957E-6	45.000004	1
ACAACGT	20	7.032501E-4	45.000004	25
AATCCGA	20	7.032501E-4	45.000004	35
ACGCCCA	20	7.032501E-4	45.000004	16
TCACCGT	20	7.032501E-4	45.000004	36
GGTCGTT	40	6.8121153E-9	45.000004	8
ATCTAGC	20	7.032501E-4	45.000004	40
GATCGGA	30	2.1649957E-6	45.000004	1
CTCCGAT	20	7.032501E-4	45.000004	19
CGCGGTA	20	7.032501E-4	45.000004	32
TTTACGG	30	2.1649957E-6	45.000004	2
TTAGCGC	20	7.032501E-4	45.000004	1
TAGACCG	20	7.032501E-4	45.000004	1
TAGCGGA	20	7.032501E-4	45.000004	3
TTGTCGT	65	0.0	45.000004	28
CCGGACG	20	7.032501E-4	45.000004	17
CCAGCGA	30	2.1649957E-6	45.000004	10
TTCGTCA	30	2.1649957E-6	45.000004	19
TACGGTG	20	7.032501E-4	45.000004	14
GGTCACG	20	7.032501E-4	45.000004	30

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content