##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934794.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	907748
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.955572471655127	33.0	31.0	34.0	30.0	34.0
2	32.13444370023399	33.0	31.0	34.0	30.0	34.0
3	32.149558027117656	34.0	31.0	34.0	30.0	34.0
4	35.761094488778824	37.0	35.0	37.0	35.0	37.0
5	35.64424487853457	37.0	35.0	37.0	33.0	37.0
6	35.55369331576605	37.0	35.0	37.0	33.0	37.0
7	35.937987194683984	37.0	35.0	37.0	35.0	37.0
8	35.77428757760965	37.0	35.0	37.0	35.0	37.0
9	37.482496243450825	39.0	37.0	39.0	35.0	39.0
10	37.19124580830803	39.0	37.0	39.0	34.0	39.0
11	37.02692156854105	39.0	37.0	39.0	33.0	39.0
12	36.903971146177135	39.0	35.0	39.0	33.0	39.0
13	36.77278385631254	39.0	35.0	39.0	33.0	39.0
14	37.770896768706734	40.0	36.0	41.0	33.0	41.0
15	37.8793751129168	40.0	36.0	41.0	33.0	41.0
16	38.00782816376351	40.0	36.0	41.0	33.0	41.0
17	38.00901571801866	40.0	36.0	41.0	33.0	41.0
18	37.9790448450451	39.0	36.0	41.0	33.0	41.0
19	37.96493410065349	40.0	36.0	41.0	34.0	41.0
20	37.89825259873886	40.0	36.0	41.0	34.0	41.0
21	37.811902642583625	39.0	36.0	41.0	33.0	41.0
22	37.753873321670774	39.0	36.0	41.0	33.0	41.0
23	37.56508193904035	39.0	35.0	41.0	33.0	41.0
24	37.25983753200227	39.0	35.0	41.0	32.0	41.0
25	37.21555101195486	39.0	35.0	41.0	32.0	41.0
26	37.4260455544931	39.0	35.0	41.0	33.0	41.0
27	37.51822091593702	39.0	35.0	41.0	33.0	41.0
28	37.51666762141035	39.0	36.0	41.0	33.0	41.0
29	37.53703891388359	39.0	36.0	41.0	33.0	41.0
30	37.41294720561213	39.0	36.0	41.0	33.0	41.0
31	37.302293147437396	39.0	35.0	41.0	33.0	41.0
32	37.188416829340305	39.0	35.0	41.0	32.0	41.0
33	37.11165764066679	39.0	35.0	41.0	32.0	41.0
34	37.0463476647704	39.0	35.0	41.0	32.0	41.0
35	36.981222762264416	39.0	35.0	41.0	32.0	41.0
36	36.957350498155876	39.0	35.0	41.0	32.0	41.0
37	36.811143621357466	39.0	35.0	41.0	31.0	41.0
38	36.72659923238608	39.0	35.0	41.0	31.0	41.0
39	36.773408479005184	39.0	35.0	41.0	31.0	41.0
40	36.71943755315352	39.0	35.0	41.0	31.0	41.0
41	36.668716427907306	39.0	35.0	41.0	31.0	41.0
42	36.62179371367384	39.0	35.0	41.0	31.0	41.0
43	36.53532257851298	39.0	35.0	41.0	31.0	41.0
44	36.4655895689112	39.0	35.0	41.0	31.0	41.0
45	36.433771266915485	39.0	35.0	40.0	30.0	41.0
46	36.392140770345954	39.0	35.0	40.0	30.0	41.0
47	36.26566073403632	39.0	35.0	40.0	30.0	41.0
48	36.26751917933171	39.0	35.0	40.0	30.0	41.0
49	36.30908247663449	39.0	35.0	40.0	30.0	41.0
50	36.19942869606984	39.0	35.0	40.0	30.0	41.0
51	35.333801892155094	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	5.0
10	11.0
11	6.0
12	18.0
13	16.0
14	15.0
15	23.0
16	63.0
17	106.0
18	185.0
19	428.0
20	714.0
21	1137.0
22	1702.0
23	2562.0
24	3831.0
25	5436.0
26	7207.0
27	8535.0
28	9637.0
29	11239.0
30	13990.0
31	17760.0
32	23742.0
33	33367.0
34	61033.0
35	81756.0
36	71603.0
37	107928.0
38	196977.0
39	246595.0
40	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.29829864676099	22.114617713286066	24.86659293107779	12.72049070887515
2	31.297122108779092	24.904709236484134	25.54827991909649	18.249888735640287
3	30.07288366374809	24.212666951620935	28.707306433062918	17.007142951568056
4	28.15891635123404	25.032828494251707	27.786786641226417	19.02146851328783
5	24.18920228962223	28.142061453178634	27.623415309094597	20.04532094810454
6	24.19812547094568	31.991918461951997	27.56712215284418	16.242833914258142
7	74.9266316202294	5.58062369732569	12.732388283973084	6.7603563984718225
8	73.76055909789942	4.49893582800513	12.75970864160538	8.980796432490076
9	68.73416410721919	6.382057575450456	15.863984277574833	9.019794039755528
10	37.7463789509864	25.807382665673735	21.95003459109797	14.496203792241898
11	25.784909468266527	24.111207075091325	29.535509855158036	20.56837360148411
12	24.0635066119672	20.464159656644796	34.66237325777639	20.809960473611618
13	21.154549500522172	25.378408985753754	34.99858991702543	18.468451596698642
14	16.392875555770985	30.513093942371672	31.31827335339764	21.775757148459704
15	14.18730749062515	27.055195935435826	39.99799503827053	18.75950153566849
16	15.956741298245769	27.28411409333868	33.846177573511596	22.91296703490396
17	16.287449820875395	27.44351956710453	29.327522616408956	26.94150799561112
18	17.431710122192502	26.083009822109222	34.451411625252824	22.033868430445455
19	18.75696779282356	28.19890542309099	29.620996135491346	23.423130648594103
20	20.732405910010268	28.324490938013636	30.73132631523286	20.211776836743237
21	19.753059219078423	28.35908203598356	32.2184130397423	19.669445705195717
22	17.106179247985125	26.2477031070297	29.21130093373932	27.43481671124585
23	17.632536783336345	26.925093748485263	30.3642640909151	25.07810537726329
24	21.16677756381727	23.948056068424275	30.17863988684084	24.706526480917613
25	17.193758620233808	27.05189105346418	28.015704799129278	27.73864552717274
26	15.856933862701982	27.68664871748547	29.97186443814803	26.484552981664518
27	19.67506400454752	27.517659086001846	30.97368432648709	21.833592582963554
28	16.798384573692257	26.493476162987967	31.390540105844355	25.317599157475424
29	17.690482380572583	25.27871171294236	29.943993266853795	27.086812639631262
30	19.957411087658688	25.087248884051522	28.909014396065867	26.046325632223922
31	20.13367145947994	27.064780093153608	24.68603621269339	28.115512234673055
32	18.71962262654393	28.28362056429758	27.17373103548562	25.82302577367287
33	19.638489977394606	24.18281285114371	29.64390998382811	26.534787187633572
34	18.17145287017983	22.584131278724932	30.020997016793206	29.223418834302027
35	15.6800125144864	23.602916227851782	31.077788108594017	29.6392831490678
36	20.30409320648462	21.961711840731127	31.91392324742109	25.82027170536316
37	19.383683577380506	23.86433239180918	34.19913896808365	22.55284506272666
38	18.198773227812122	26.81107532046339	29.496952898822137	25.493198552902346
39	19.545512631258894	23.064661117402625	31.223863891740876	26.1659623595976
40	20.510428004247874	20.641852143987098	31.938048885814123	26.909670965950905
41	16.240079845948436	22.317427303612895	31.131767847464275	30.310725002974394
42	20.119460467001854	20.061184381568452	34.244526013827624	25.574829137602066
43	22.71654688305565	21.085477467314718	32.49690442721989	23.701071222409745
44	20.057769336864418	22.957582941521217	28.99108563169514	27.993562089919227
45	19.831384921806492	21.52822148878323	27.25525145745295	31.385142131957327
46	22.614536192864097	21.546949153289237	29.714303969824226	26.124210684022437
47	16.37458854219453	21.39228067701609	35.49355107364599	26.739579707143392
48	18.01733520756862	19.638710302859383	33.581456527582546	28.762497961989453
49	19.76616858423263	17.824660588621512	36.83357055041708	25.575600276728782
50	18.99436848112031	17.883707813181633	34.31536065075329	28.806563054944768
51	17.05252999731203	18.619374540070595	29.467539449274465	34.86055601334291
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1151.0
1	1164.5
2	1178.0
3	3810.5
4	6443.0
5	4321.0
6	2199.0
7	2201.5
8	2204.0
9	2337.5
10	2471.0
11	2581.5
12	2692.0
13	2694.0
14	2696.0
15	2553.0
16	2410.0
17	2333.5
18	2257.0
19	2378.0
20	2499.0
21	2443.5
22	2388.0
23	2851.5
24	3315.0
25	4155.0
26	5726.0
27	6457.0
28	6921.0
29	7385.0
30	10456.5
31	13528.0
32	14936.0
33	16344.0
34	16932.0
35	17520.0
36	19666.5
37	21813.0
38	23276.0
39	24739.0
40	29825.0
41	34911.0
42	41189.0
43	47467.0
44	59858.5
45	72250.0
46	89094.0
47	105938.0
48	123864.0
49	141790.0
50	137683.0
51	133576.0
52	103893.0
53	74210.0
54	59522.5
55	44835.0
56	37353.5
57	29872.0
58	25963.0
59	22054.0
60	19682.0
61	17310.0
62	16649.5
63	15989.0
64	12989.0
65	9989.0
66	7437.0
67	4885.0
68	3524.0
69	2163.0
70	1914.5
71	1666.0
72	1300.0
73	934.0
74	867.5
75	526.0
76	251.0
77	163.0
78	75.0
79	47.0
80	19.0
81	35.0
82	51.0
83	29.5
84	8.0
85	7.5
86	7.0
87	5.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	4.5
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	907748.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.46956052489964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.59386570535973	15.159896407091795
2	12.493630430383618	5.864400311232325
3	6.254546005896858	4.4037433812349684
4	3.6883371058798886	3.462546037707249
5	2.3881740692192452	2.8024697930768467
6	1.681169956770735	2.3673792031844205
7	1.246582892223103	2.0479726846834194
8	0.906333969738132	1.701700796683332
9	0.6786963601077063	1.4335834771419205
>10	4.516599165902972	21.6406727320586
>50	1.2046367007263217	20.259070459900936
>100	0.32802091687963014	11.425672712739036
>500	0.010886697097015141	1.7729894361291094
>1k	0.00710001984587944	3.0126378770918114
>5k	0.001420003969175888	2.6452646900442573
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	9706	1.0692394805606842	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	7988	0.8799799063176125	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	6118	0.6739755967515214	TruSeq Adapter, Index 16 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4023	0.4431846723980664	No Hit
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	3928	0.4327192128211794	TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	2965	0.326632501531262	TruSeq Adapter, Index 16 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	2266	0.24962875159185147	TruSeq Adapter, Index 16 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	1525	0.16799816689213307	TruSeq Adapter, Index 16 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	1506	0.16590507497675566	No Hit
ACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	1498	0.16502377311764937	TruSeq Adapter, Index 16 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	1443	0.15896482283629376	No Hit
GGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	1363	0.15015180424523106	No Hit
AAAACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	1222	0.13461885897848302	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	1114	0.12272128388054834	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	1107	0.12195014475383036	No Hit
GGTTGGAGGGAAATGACAGAAATACTGAAGAAAAAATAAGTCAAAATCATA	1089	0.11996721557084125	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCC	1054	0.11611151993725131	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	1016	0.11192533610649652	No Hit
GGCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	939	0.10344280571259865	No Hit
AGCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	926	0.10201069019155096	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	919	0.10123955106483297	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1016273238828398E-4	0.0	0.0	0.711430925763538	0.0
2	2.2032546477656796E-4	0.0	0.0	3.5754416423941446	0.0
3	2.2032546477656796E-4	0.0	0.0	4.974398180992963	0.0
4	2.2032546477656796E-4	0.0	0.0	6.544767931187951	0.0
5	2.2032546477656796E-4	0.0	0.0	10.335798040865967	0.0
6	2.2032546477656796E-4	0.0	0.0	12.147314012258908	0.0
7	2.2032546477656796E-4	0.0	0.0	14.138395237444753	0.0
8	2.2032546477656796E-4	0.0	0.0	17.119619101336493	0.0
9	2.2032546477656796E-4	0.0	0.0	18.092356028325042	0.0
10	2.2032546477656796E-4	0.0	0.0	20.351132693214417	0.0
11	2.2032546477656796E-4	0.0	0.0	23.497490492956196	0.0
12	2.2032546477656796E-4	0.0	0.0	26.044122377576155	0.0
13	2.2032546477656796E-4	0.0	0.0	26.95924419552563	0.0
14	2.2032546477656796E-4	0.0	0.0	27.309231196323207	0.0
15	3.304881971648519E-4	0.0	0.0	27.950157973358245	0.0
16	3.304881971648519E-4	0.0	0.0	29.30449860533981	0.0
17	3.304881971648519E-4	0.0	0.0	31.0741527384252	0.0
18	3.304881971648519E-4	0.0	0.0	33.161406028986015	0.0
19	3.304881971648519E-4	0.0	0.0	34.3106236532606	0.0
20	3.304881971648519E-4	0.0	0.0	35.328637463260726	0.0
21	3.304881971648519E-4	0.0	0.0	36.605313368908554	0.0
22	4.406509295531359E-4	0.0	0.0	37.810273335771605	0.0
23	4.406509295531359E-4	0.0	0.0	38.95244054517333	0.0
24	4.406509295531359E-4	0.0	0.0	39.811048881407615	0.0
25	4.406509295531359E-4	0.0	0.0	40.57458677959081	0.0
26	4.406509295531359E-4	0.0	0.0	41.28403477617136	0.0
27	4.406509295531359E-4	0.0	0.0	41.95371402635974	0.0
28	5.508136619414199E-4	0.0	0.0	42.62130018463274	0.0
29	5.508136619414199E-4	0.0	0.0	43.340552664395844	0.0
30	5.508136619414199E-4	0.0	0.0	44.08415110801676	0.0
31	5.508136619414199E-4	0.0	0.0	44.86839959988895	0.0
32	5.508136619414199E-4	0.0	0.0	45.54975609971049	0.0
33	5.508136619414199E-4	0.0	0.0	46.189801574886424	0.0
34	6.609763943297038E-4	0.0	0.0	46.82544054076682	0.0
35	6.609763943297038E-4	0.0	0.0	47.48234091399816	0.0
36	6.609763943297038E-4	0.0	0.0	48.1015656327527	0.0
37	6.609763943297038E-4	0.0	0.0	48.74744973274521	0.0
38	6.609763943297038E-4	0.0	0.0	49.352793947218835	0.0
39	6.609763943297038E-4	0.0	0.0	50.031616704195436	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCG	30	2.1649957E-6	45.000004	1
ACAACGT	20	7.032501E-4	45.000004	25
AATCCGA	20	7.032501E-4	45.000004	35
ACGCCCA	20	7.032501E-4	45.000004	16
TCACCGT	20	7.032501E-4	45.000004	36
GGTCGTT	40	6.8121153E-9	45.000004	8
ATCTAGC	20	7.032501E-4	45.000004	40
GATCGGA	30	2.1649957E-6	45.000004	1
CTCCGAT	20	7.032501E-4	45.000004	19
CGCGGTA	20	7.032501E-4	45.000004	32
TTTACGG	30	2.1649957E-6	45.000004	2
TTAGCGC	20	7.032501E-4	45.000004	1
TAGACCG	20	7.032501E-4	45.000004	1
TAGCGGA	20	7.032501E-4	45.000004	3
TTGTCGT	65	0.0	45.000004	28
CCGGACG	20	7.032501E-4	45.000004	17
CCAGCGA	30	2.1649957E-6	45.000004	10
TTCGTCA	30	2.1649957E-6	45.000004	19
TACGGTG	20	7.032501E-4	45.000004	14
GGTCACG	20	7.032501E-4	45.000004	30
>>END_MODULE