##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934793.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	859394
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12628782607279	33.0	31.0	34.0	30.0	34.0
2	32.302779633090296	34.0	31.0	34.0	30.0	34.0
3	32.309873003535046	34.0	31.0	34.0	30.0	34.0
4	35.89314563518014	37.0	35.0	37.0	35.0	37.0
5	35.77925840766866	37.0	35.0	37.0	35.0	37.0
6	35.70870171306758	37.0	35.0	37.0	33.0	37.0
7	36.02284865847329	37.0	35.0	37.0	35.0	37.0
8	35.85726919201205	37.0	35.0	37.0	35.0	37.0
9	37.557883811150646	39.0	38.0	39.0	35.0	39.0
10	37.34909482728527	39.0	37.0	39.0	34.0	39.0
11	37.228308552305464	39.0	37.0	39.0	34.0	39.0
12	37.047646364764006	39.0	37.0	39.0	33.0	39.0
13	36.88667945086887	39.0	35.0	39.0	33.0	39.0
14	37.98818120675732	40.0	37.0	41.0	33.0	41.0
15	38.05574160396745	40.0	37.0	41.0	33.0	41.0
16	38.17338845744792	40.0	37.0	41.0	34.0	41.0
17	38.18139991668548	40.0	37.0	41.0	34.0	41.0
18	38.1537490371122	40.0	37.0	41.0	34.0	41.0
19	38.13506726833094	40.0	37.0	41.0	34.0	41.0
20	38.063480778315885	40.0	36.0	41.0	34.0	41.0
21	37.97963797745854	40.0	36.0	41.0	34.0	41.0
22	37.91245459009488	40.0	36.0	41.0	34.0	41.0
23	37.7560455390659	39.0	35.0	41.0	33.0	41.0
24	37.49453684805805	39.0	35.0	41.0	33.0	41.0
25	37.43190201467546	39.0	35.0	41.0	33.0	41.0
26	37.634840364256675	39.0	35.0	41.0	33.0	41.0
27	37.71830615526755	39.0	35.0	41.0	33.0	41.0
28	37.65119258454213	39.0	36.0	41.0	33.0	41.0
29	37.643915363616685	39.0	36.0	41.0	33.0	41.0
30	37.52061452604975	39.0	36.0	41.0	33.0	41.0
31	37.395286678752704	39.0	35.0	41.0	33.0	41.0
32	37.26475632829645	39.0	35.0	41.0	33.0	41.0
33	37.05220771846208	39.0	35.0	41.0	32.0	41.0
34	36.99490222179815	39.0	35.0	41.0	32.0	41.0
35	36.941205081720376	39.0	35.0	41.0	32.0	41.0
36	36.78053605214838	39.0	35.0	41.0	31.0	41.0
37	36.68393542426407	39.0	35.0	41.0	31.0	41.0
38	36.59491804690282	39.0	35.0	40.0	31.0	41.0
39	36.625536133601116	39.0	35.0	40.0	31.0	41.0
40	36.670107075450844	39.0	35.0	41.0	31.0	41.0
41	36.60600143822275	39.0	35.0	41.0	31.0	41.0
42	36.42936185265431	39.0	35.0	40.0	31.0	41.0
43	36.35631968573204	39.0	35.0	40.0	31.0	41.0
44	36.33840822719265	39.0	35.0	40.0	31.0	41.0
45	36.366212703370046	39.0	35.0	40.0	31.0	41.0
46	36.349549799044446	39.0	35.0	40.0	31.0	41.0
47	36.17295559429086	38.0	35.0	40.0	30.0	41.0
48	36.1233683269839	38.0	35.0	40.0	30.0	41.0
49	36.08242319587989	38.0	35.0	40.0	30.0	41.0
50	36.013708496917594	38.0	35.0	40.0	30.0	41.0
51	35.078148090398585	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	13.0
10	8.0
11	12.0
12	20.0
13	14.0
14	19.0
15	19.0
16	37.0
17	94.0
18	164.0
19	335.0
20	603.0
21	933.0
22	1477.0
23	2289.0
24	3080.0
25	4714.0
26	6315.0
27	7634.0
28	8613.0
29	9901.0
30	12263.0
31	15903.0
32	21575.0
33	30904.0
34	57917.0
35	81543.0
36	69766.0
37	106341.0
38	188856.0
39	227890.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.873801771946276	20.948249580518365	23.97619718080415	14.201751466731208
2	32.064338359355546	23.831909461783535	25.371366334882488	18.73238584397843
3	31.410505542277466	23.0543848339644	27.848460659487966	17.686648964270173
4	28.630290646664974	25.873697046988926	26.81622166317196	18.679790643174144
5	23.496091431869434	28.48158120722276	27.1061934339779	20.916133926929906
6	25.05986776728718	32.113675450375496	26.435953706914404	16.390503075422913
7	75.82145092937581	5.921498171967689	11.772946983572146	6.484103915084351
8	74.66156384615206	4.836198530592487	12.078860220108588	8.42337740314687
9	68.95230825442114	6.6444494608991915	15.466712590499817	8.936529694179853
10	38.616280774592326	24.32644398262031	22.109532996506843	14.947742246280518
11	27.789000155923826	24.30386993625741	26.93933166859438	20.967798239224383
12	25.8333197578759	20.644430843128998	33.137071005848306	20.3851783931468
13	22.18505132686521	26.261412111324955	32.76622829575258	18.787308266057245
14	16.98510811106431	28.93108399639746	31.30880597258068	22.775001919957553
15	16.06853201209224	25.99959971794078	37.68364684882604	20.248221421140943
16	17.556091850769263	27.01764266448218	32.22142579538605	23.204839689362505
17	18.166987435332338	26.04498053279404	29.095153096251547	26.692878935622076
18	18.963129833347683	25.349374093838218	33.87305473391716	21.814441338896945
19	20.66328133545266	26.3098183138351	29.826366020707617	23.200534330004633
20	22.4836338163869	27.07396141932571	29.476817385273808	20.965587379013584
21	20.52946611216741	26.889994577574434	31.847557697633448	20.73298161262471
22	19.313725718355027	25.361824727656927	28.718957777224418	26.60549177676363
23	18.759963416081565	25.46503699118216	29.857783507913716	25.91721608482256
24	21.932780540706588	23.058806554385995	30.917483715269135	24.09092918963828
25	17.837801986050636	26.298182207462467	29.337533191993426	26.526482614493467
26	18.54004100563886	26.611891635268574	30.975198802877372	23.87286855621519
27	21.9580308915352	26.184846531393053	30.317526070696328	21.53959650637542
28	17.009078490191925	24.752441836922294	34.84024789561016	23.398231777275615
29	18.231916908891616	24.49004763821949	33.273678894662986	24.00435655822591
30	24.865661151927988	24.749416449265414	29.52964530820555	20.855277090601053
31	24.683556087196326	26.29282959853106	27.994028350209565	21.02958596406305
32	25.431525004828988	27.098397242708234	27.297258300616477	20.1728194518463
33	26.503326762811934	23.962815658475623	28.39826668559473	21.13559089311771
34	22.602554823515174	23.236257176568607	31.94972271158514	22.21146528833108
35	24.21078108527637	23.265231081436454	29.288428823100933	23.235559010186247
36	27.972385192356477	21.58811906994929	31.496147285180022	18.943348452514215
37	24.63096088639204	23.339818523285015	33.197113314731084	18.83210727559187
38	22.464899685126962	27.110382432272047	28.481232124031585	21.943485758569413
39	24.81341503431488	23.883806496205466	30.780410382199552	20.522368087280107
40	27.88499803349802	20.67131024884977	30.091436523876126	21.352255193776077
41	23.860301561332754	21.734734010244427	28.903157341103146	25.501807087319666
42	23.60814713623786	20.814550718296847	32.357451878882095	23.219850266583197
43	24.714159046956343	21.54785814189999	31.256792577095023	22.481190234048643
44	21.40659581053626	22.94198004640479	30.56816780196278	25.083256341096167
45	23.452921477226976	22.442791083018964	27.985766714685	26.11852072506906
46	27.18601712369414	21.693309471557864	29.599345585377602	21.52132781937039
47	22.05798504527609	21.850978712907	34.01338617677107	22.077650065045834
48	22.472579515332896	20.1121953376449	32.76297018596826	24.652254961053952
49	22.899391897080967	18.60857767217365	35.96976474120136	22.522265689544028
50	22.683309401741226	19.673281405269293	32.68605552284517	24.95735367014431
51	21.423468164776573	19.233552945447606	29.121916140908592	30.221062748867222
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	855.0
1	1004.5
2	1154.0
3	4133.5
4	7113.0
5	4639.5
6	2166.0
7	2163.0
8	2160.0
9	2263.0
10	2366.0
11	2434.0
12	2502.0
13	2395.0
14	2288.0
15	2247.0
16	2206.0
17	2130.0
18	2054.0
19	2050.5
20	2047.0
21	2294.5
22	2542.0
23	3025.5
24	3509.0
25	3641.0
26	4565.5
27	5358.0
28	6324.5
29	7291.0
30	8125.5
31	8960.0
32	10847.0
33	12734.0
34	14091.5
35	15449.0
36	18373.5
37	21298.0
38	23132.5
39	24967.0
40	29999.5
41	35032.0
42	41589.0
43	48146.0
44	56366.5
45	64587.0
46	77061.0
47	89535.0
48	105430.5
49	121326.0
50	118970.5
51	116615.0
52	93124.0
53	69633.0
54	57801.0
55	45969.0
56	38939.5
57	31910.0
58	30295.0
59	28680.0
60	25366.5
61	22053.0
62	20091.0
63	18129.0
64	15397.5
65	12666.0
66	10004.5
67	7343.0
68	6558.0
69	5773.0
70	4790.5
71	3808.0
72	2969.0
73	2130.0
74	1810.0
75	1177.5
76	865.0
77	664.5
78	464.0
79	342.0
80	220.0
81	158.0
82	96.0
83	61.5
84	27.0
85	38.0
86	49.0
87	45.5
88	42.0
89	27.0
90	12.0
91	6.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	859394.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.860180126104385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.54944342961852	15.401613472704835
2	13.09310936978486	6.248078959477037
3	6.36758549835731	4.557952108775268
4	3.550658185102444	3.388773754510848
5	2.3087178388088945	2.7543211747165324
6	1.6089803734024823	2.303433691724995
7	1.1454777926106738	1.9131914523500184
8	0.8271434766023557	1.5788633873491529
9	0.6176297255826784	1.3263080853275215
>10	4.260370102552208	21.759422792950566
>50	1.3841236856492891	23.549814880467267
>100	0.2720044058770959	9.378450505408708
>500	0.009345540165759173	1.457156605162675
>1k	0.004426834815359608	2.527960154252901
>5k	9.837410700799128E-4	1.854658974821662
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	8458	0.9841818769970468	No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	7345	0.8546720130696748	No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT	4995	0.5812235133128693	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4536	0.5278137850624975	No Hit
TCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	2809	0.3268582280071772	No Hit
ACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	2207	0.25680886764394445	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG	2125	0.2472672604183878	No Hit
GCCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG	1467	0.17070168048648235	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG	1212	0.1410296092362758	No Hit
GGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG	1161	0.13509519498623448	No Hit
AGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG	1028	0.11961917351063656	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCC	977	0.11368475926059526	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3272212745260033E-4	0.0	0.0	0.6084520022248235	0.0
2	2.3272212745260033E-4	0.0	0.0	3.1809623990858675	0.0
3	2.3272212745260033E-4	0.0	0.0	4.384833964398169	0.0
4	2.3272212745260033E-4	0.0	0.0	5.640835286259853	0.0
5	2.3272212745260033E-4	0.0	0.0	8.69345143205561	0.0
6	2.3272212745260033E-4	0.0	0.0	10.185782074345411	0.0
7	2.3272212745260033E-4	0.0	0.0	11.830196626925485	0.0
8	2.3272212745260033E-4	0.0	0.0	14.153345264221068	0.0
9	2.3272212745260033E-4	0.0	0.0	14.998941114320091	0.0
10	2.3272212745260033E-4	0.0	0.0	16.815919124406268	0.0
11	2.3272212745260033E-4	0.0	0.0	19.565647421322467	0.0
12	2.3272212745260033E-4	0.0	0.0	21.651186766488944	0.0
13	2.3272212745260033E-4	0.0	0.0	22.464201518744602	0.0
14	2.3272212745260033E-4	0.0	0.0	22.766973006560438	0.0
15	2.3272212745260033E-4	0.0	0.0	23.361694403265556	0.0
16	2.3272212745260033E-4	0.0	0.0	24.699730275054282	0.0
17	2.3272212745260033E-4	0.0	0.0	26.283171630241775	0.0
18	2.3272212745260033E-4	0.0	0.0	28.14425048348022	0.0
19	2.3272212745260033E-4	0.0	0.0	29.131574109197878	0.0
20	2.3272212745260033E-4	0.0	0.0	30.051990123272912	0.0
21	2.3272212745260033E-4	0.0	0.0	31.226189617335006	0.0
22	2.3272212745260033E-4	0.0	0.0	32.32556894742109	0.0
23	2.3272212745260033E-4	0.0	0.0	33.432046302394475	0.0
24	2.3272212745260033E-4	0.0	0.0	34.281016623341564	0.0
25	2.3272212745260033E-4	0.0	0.0	35.051908670528306	0.0
26	2.3272212745260033E-4	0.0	0.0	35.717610316106466	0.0
27	2.3272212745260033E-4	0.0	0.0	36.38028657402774	0.0
28	2.3272212745260033E-4	0.0	0.0	37.01713067580179	0.0
29	2.3272212745260033E-4	0.0	0.0	37.71436616964978	0.0
30	2.3272212745260033E-4	0.0	0.0	38.49165807534146	0.0
31	2.3272212745260033E-4	0.0	0.0	39.18714815323356	0.0
32	2.3272212745260033E-4	0.0	0.0	39.848311717326396	0.0
33	2.3272212745260033E-4	0.0	0.0	40.49853734142896	0.0
34	2.3272212745260033E-4	0.0	0.0	41.12816705725197	0.0
35	2.3272212745260033E-4	0.0	0.0	41.88079041743368	0.0
36	2.3272212745260033E-4	0.0	0.0	42.52868882026172	0.0
37	2.3272212745260033E-4	0.0	0.0	43.18601246925159	0.0
38	2.3272212745260033E-4	0.0	0.0	43.81668943464813	0.0
39	2.3272212745260033E-4	0.0	0.0	44.45388261961335	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	95	0.0	45.000004	1
CAACCGG	50	2.1827873E-11	45.000004	2
CCTCCGT	25	3.8899805E-5	45.000004	30
CTCATAG	25	3.8899805E-5	45.000004	1
TTCGAAT	25	3.8899805E-5	45.000004	21
CGGAGGT	25	3.8899805E-5	45.000004	11
CGCCCGT	25	3.8899805E-5	45.000004	39
ACGGTTC	25	3.8899805E-5	45.000004	5
CGAACAT	20	7.032291E-4	45.0	40
GCGACAA	35	1.211447E-7	45.0	39
TCCGAAC	20	7.032291E-4	45.0	17
GACGTAT	20	7.032291E-4	45.0	11
GTTAGCG	20	7.032291E-4	45.0	1
TAACGCC	20	7.032291E-4	45.0	12
TCGATCT	20	7.032291E-4	45.0	35
ACGGGTC	70	0.0	45.0	5
TCCTACG	20	7.032291E-4	45.0	13
TGTTGCG	40	6.8121153E-9	45.0	1
ACGATTG	35	1.211447E-7	45.0	1
CCGTAGA	20	7.032291E-4	45.0	24
>>END_MODULE