##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934791.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	495310
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.926831681169368	33.0	31.0	34.0	30.0	34.0
2	32.108958026286565	33.0	31.0	34.0	30.0	34.0
3	32.10392683370011	33.0	31.0	34.0	30.0	34.0
4	35.744475177161775	37.0	35.0	37.0	35.0	37.0
5	35.626662090408026	37.0	35.0	37.0	33.0	37.0
6	35.56276877107266	37.0	35.0	37.0	33.0	37.0
7	35.91065797177525	37.0	35.0	37.0	35.0	37.0
8	35.72208717772708	37.0	35.0	37.0	35.0	37.0
9	37.377014395025334	39.0	37.0	39.0	35.0	39.0
10	37.188922089196666	39.0	37.0	39.0	34.0	39.0
11	37.07323696271022	39.0	37.0	39.0	33.0	39.0
12	36.85542791383174	39.0	35.0	39.0	33.0	39.0
13	36.606335426298685	39.0	35.0	39.0	33.0	39.0
14	37.55050372493994	39.0	36.0	41.0	33.0	41.0
15	37.69301649472048	39.0	36.0	41.0	33.0	41.0
16	37.833968625709154	39.0	36.0	41.0	33.0	41.0
17	37.86160788193253	39.0	36.0	41.0	33.0	41.0
18	37.83639538874644	39.0	36.0	41.0	33.0	41.0
19	37.807504391189354	39.0	36.0	41.0	34.0	41.0
20	37.71270921241243	39.0	35.0	41.0	34.0	41.0
21	37.64594294482244	39.0	35.0	41.0	33.0	41.0
22	37.55499182330258	39.0	35.0	41.0	33.0	41.0
23	37.3540590741152	39.0	35.0	40.0	33.0	41.0
24	37.070642627849224	39.0	35.0	40.0	32.0	41.0
25	37.02640568532838	39.0	35.0	40.0	32.0	41.0
26	37.23175587006117	39.0	35.0	40.0	33.0	41.0
27	37.34419252589287	39.0	35.0	40.0	33.0	41.0
28	37.3507035997658	39.0	35.0	41.0	33.0	41.0
29	37.32660556015425	39.0	35.0	41.0	33.0	41.0
30	37.130546526417795	39.0	35.0	40.0	32.0	41.0
31	37.01562052048212	39.0	35.0	40.0	32.0	41.0
32	36.82731420726414	39.0	35.0	40.0	32.0	41.0
33	36.623556964325374	39.0	35.0	40.0	31.0	41.0
34	36.60941430619208	39.0	35.0	40.0	31.0	41.0
35	36.48831640790616	39.0	35.0	40.0	31.0	41.0
36	36.37635218348105	39.0	35.0	40.0	31.0	41.0
37	36.308766227211244	38.0	35.0	40.0	30.0	41.0
38	36.14424299933375	38.0	35.0	40.0	30.0	41.0
39	36.189939633764716	38.0	35.0	40.0	30.0	41.0
40	36.07553249480124	38.0	35.0	40.0	30.0	41.0
41	36.05251862469968	38.0	35.0	40.0	30.0	41.0
42	35.9673315701278	38.0	35.0	40.0	30.0	41.0
43	35.90798489834649	38.0	35.0	40.0	30.0	41.0
44	35.85422462700127	38.0	35.0	40.0	30.0	41.0
45	35.82373866871252	38.0	35.0	40.0	30.0	41.0
46	35.71051260826553	38.0	35.0	40.0	29.0	41.0
47	35.54404110557025	38.0	35.0	40.0	28.0	41.0
48	35.532424138418364	38.0	35.0	40.0	28.0	41.0
49	35.55660697341059	38.0	35.0	40.0	28.0	41.0
50	35.43744119844138	38.0	35.0	40.0	28.0	41.0
51	34.387952999131855	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	9.0
11	3.0
12	5.0
13	7.0
14	7.0
15	16.0
16	32.0
17	65.0
18	148.0
19	254.0
20	427.0
21	799.0
22	1167.0
23	1760.0
24	2592.0
25	3538.0
26	4512.0
27	5193.0
28	5901.0
29	6762.0
30	8338.0
31	10890.0
32	14084.0
33	20138.0
34	38114.0
35	51459.0
36	41008.0
37	61638.0
38	107830.0
39	108560.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.332115240960206	22.033675879752074	24.17294219781551	13.461266681472209
2	32.600593567664696	24.28337808645091	23.762694070380167	19.35333427550423
3	28.439764995659285	25.441642607659848	29.875229654155984	16.243362742524884
4	28.01498051725182	26.00250348266742	27.574448325291236	18.408067674789525
5	22.770184329006078	28.67981668046274	27.815509478912197	20.734489511618985
6	23.461064787708708	31.252952696291214	28.338414326381457	16.94756818961862
7	71.89961842078698	6.567806020472028	14.426722658537077	7.105852900203913
8	68.8106438392118	5.643334477397993	14.7604530496053	10.785568633784901
9	62.898184975066115	7.683067170055118	18.532030445579537	10.886717409299226
10	36.944135995639094	22.7610991096485	24.983141870747613	15.31162302396479
11	27.510044214734208	23.0429428034968	28.566554279138316	20.880458702630676
12	25.254890876420827	20.19906725081262	36.05015041085381	18.49589146191274
13	21.108800549151034	27.756556499969715	34.48688700006057	16.64775595081868
14	14.786699238860512	33.08069693727161	31.22973491348852	20.902868910379357
15	13.014879570370072	26.10022006420222	42.01348650340191	18.8714138620258
16	13.033857584139225	26.86257091518443	36.26133128747653	23.842240213199815
17	14.37079808604712	26.134340110233996	29.536250025236722	29.958611778482165
18	16.80281036118794	25.382083947426864	35.706123437847005	22.108982253538187
19	17.963295713795404	27.246976640891564	30.415093577759382	24.374634067553654
20	19.89077547394561	27.795521996325533	31.131412650663222	21.182289879065635
21	19.54210494437827	27.410308695564396	32.99125800004038	20.05632836001696
22	15.780016555288606	27.287557287355398	28.262300377541337	28.670125779814658
23	14.84787304920151	28.151864488905936	29.68201732248491	27.318245139407644
24	20.915992005006963	23.951262845490703	31.113242211948073	24.01950293755426
25	14.947608568371324	26.831681169368675	30.742969049686053	27.477741212573942
26	14.58278653772385	27.623912297349136	34.04130746401244	23.75199370091458
27	18.746441622418285	27.150269528174277	29.630736306555487	24.472552542851954
28	14.096424461448386	24.419252589287517	31.34844844642749	30.135874502836607
29	18.064848276836727	22.23052229916618	33.70353919767418	26.00109022632291
30	19.77064868466213	26.518342048414123	26.961296965536736	26.749712301387007
31	22.27150673315701	27.414548464597928	23.60945670388242	26.704488098362646
32	22.83317518321859	30.43447537905554	22.830954351820072	23.901395085905797
33	20.285477781591325	31.382366598695764	22.48955199773879	25.84260362197412
34	20.173830530374918	24.975066120207547	28.59037774323151	26.260725606186025
35	18.826593446528435	27.699622458662255	26.37802588278048	27.09575821202883
36	21.267892834790334	28.41129797500555	26.288385051785752	24.032424138418364
37	21.458480547535885	26.354000524923787	28.857685086107693	23.329833841432638
38	19.173850719751268	32.22850336153116	23.481859845349376	25.115786073368195
39	23.747955825644546	28.11653308029315	27.50479497688316	20.63071611717914
40	24.534332034483455	25.075609214431367	29.218873029012133	21.171185722073048
41	18.829218065453958	27.93604005572268	24.643556560537842	28.59118531828552
42	20.371484524843027	25.03603803678504	28.132482687609777	26.45999475076215
43	23.098665482223254	23.23413619753286	27.84276513698492	25.82443318325897
44	20.198461569522117	23.508307928368094	27.13351234580364	29.159718156306152
45	22.63935717025701	21.80250752054269	25.741252952696293	29.81688235650401
46	24.17819143566655	24.667783812158042	28.590983424522015	22.563041327653387
47	18.057176313823668	24.0734085724092	32.74050594577134	25.1289091679958
48	18.560497466233265	22.66136359047869	29.90268720599221	28.875451737295837
49	21.191778885950214	19.59096323514567	34.861198037592615	24.356059841311502
50	21.052270295370576	19.436514506066906	32.08132280793846	27.429892390624055
51	18.606933031838647	20.103571500676345	26.49875835335446	34.79073711413054
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1164.0
1	1179.5
2	1195.0
3	2922.0
4	4649.0
5	3012.5
6	1376.0
7	1436.5
8	1497.0
9	1588.5
10	1680.0
11	1737.0
12	1794.0
13	1771.5
14	1749.0
15	1849.5
16	1950.0
17	1833.5
18	1717.0
19	1694.0
20	1671.0
21	1589.0
22	1507.0
23	1621.5
24	1736.0
25	2015.5
26	2406.5
27	2518.0
28	2745.0
29	2972.0
30	3390.5
31	3809.0
32	4396.0
33	4983.0
34	5890.0
35	6797.0
36	8465.0
37	10133.0
38	11286.0
39	12439.0
40	15834.5
41	19230.0
42	23778.0
43	28326.0
44	34314.0
45	40302.0
46	51973.5
47	63645.0
48	74017.0
49	84389.0
50	81423.0
51	78457.0
52	58750.0
53	39043.0
54	31215.0
55	23387.0
56	19365.0
57	15343.0
58	12770.0
59	10197.0
60	9042.5
61	7888.0
62	6890.5
63	5893.0
64	4664.5
65	3436.0
66	2764.5
67	2093.0
68	1951.0
69	1809.0
70	1313.5
71	818.0
72	694.0
73	570.0
74	443.5
75	214.0
76	111.0
77	188.5
78	266.0
79	203.0
80	140.0
81	73.5
82	7.0
83	6.0
84	5.0
85	3.5
86	2.0
87	2.0
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	495310.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.300947268647022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.2046485354647	17.412449700705665
2	12.27684483708028	6.4578529737081976
3	5.984437601871825	4.721891333980179
4	3.5310516280212116	3.714800106858244
5	2.3198619305308124	3.0507283152716287
6	1.5943349118956154	2.515951106779774
7	1.1589747088410163	2.1337492892046175
8	0.8418402750059445	1.7712957345243723
9	0.6374963712268866	1.5090082599232955
>10	4.266522580843913	23.15814495649447
>50	0.9989887099650127	18.27162046659679
>100	0.16881059219316186	8.01205468832418
>500	0.01002072008452559	1.9953097733422722
>1k	0.004624947731319503	2.735694988973849
>5k	0.0015416492437731676	2.539448305312467
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	6275	1.2668833659728251	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	6251	1.2620379156487858	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	3813	0.7698209202317741	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2952	0.5959903898568574	No Hit
TCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	2144	0.4328602289475278	No Hit
ACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	1786	0.36058226161393875	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	1687	0.34059477902727586	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	1112	0.22450586501382971	No Hit
ACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	993	0.20048050715713392	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	990	0.19987482586662897	No Hit
AGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	978	0.19745210070460922	No Hit
AAAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	944	0.1905877127455533	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCC	887	0.1790797682259595	No Hit
GGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	861	0.17383053037491672	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	782	0.15788092305828672	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	714	0.14415214714017485	No Hit
GTATTAGGGAGATCTTCCTTGAAGTATTTTTTAAAAAATATATTTCCCTTT	549	0.11083967616240334	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	542	0.10942641981789183	No Hit
GAAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	540	0.10902263229088853	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	531	0.10720558841937372	No Hit
GGCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	531	0.10720558841937372	No Hit
TGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	500	0.10094688175082273	No Hit
GGACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	497	0.10034120046031779	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0189376350164542E-4	0.0	0.0	0.8158526983101492	0.0
2	2.0189376350164542E-4	0.0	0.0	4.353839009912984	0.0
3	2.0189376350164542E-4	0.0	0.0	6.061254567846399	0.0
4	2.0189376350164542E-4	0.0	0.0	7.814096222567685	0.0
5	2.0189376350164542E-4	0.0	0.0	12.230118511639175	0.0
6	2.0189376350164542E-4	0.0	0.0	14.283983767741415	0.0
7	2.0189376350164542E-4	0.0	0.0	16.566998445418022	0.0
8	2.0189376350164542E-4	0.0	0.0	20.005854919141548	0.0
9	2.0189376350164542E-4	0.0	0.0	21.23680119521108	0.0
10	2.0189376350164542E-4	0.0	0.0	23.80610122953302	0.0
11	2.0189376350164542E-4	0.0	0.0	27.560315761846116	0.0
12	2.0189376350164542E-4	0.0	0.0	30.35715006763441	0.0
13	2.0189376350164542E-4	0.0	0.0	31.44192525892875	0.0
14	2.0189376350164542E-4	0.0	0.0	31.855807474107124	0.0
15	2.0189376350164542E-4	0.0	0.0	32.59857463002968	0.0
16	2.0189376350164542E-4	0.0	0.0	34.23754820213603	0.0
17	2.0189376350164542E-4	0.0	0.0	36.27324301952313	0.0
18	2.0189376350164542E-4	0.0	0.0	38.851830166966145	0.0
19	2.0189376350164542E-4	0.0	0.0	40.09186166239325	0.0
20	2.0189376350164542E-4	0.0	0.0	41.18693343562617	0.0
21	2.0189376350164542E-4	0.0	0.0	42.49702206698835	0.0
22	2.0189376350164542E-4	0.0	0.0	43.72897781187539	0.0
23	2.0189376350164542E-4	0.0	0.0	44.8961256586784	0.0
24	2.0189376350164542E-4	0.0	0.0	45.838565746704084	0.0
25	2.0189376350164542E-4	0.0	0.0	46.61464537360441	0.0
26	2.0189376350164542E-4	0.0	0.0	47.29765197553048	0.0
27	2.0189376350164542E-4	0.0	0.0	47.96814116411944	0.0
28	2.0189376350164542E-4	0.0	0.0	48.657204578950555	0.0
29	2.0189376350164542E-4	0.0	0.0	49.384627808846986	0.0
30	2.0189376350164542E-4	0.0	0.0	50.236417597060424	0.0
31	2.0189376350164542E-4	0.0	0.0	51.03349417536492	0.0
32	2.0189376350164542E-4	0.0	0.0	51.65754779834851	0.0
33	2.0189376350164542E-4	0.0	0.0	52.30138701015526	0.0
34	4.0378752700329085E-4	0.0	0.0	52.885263774202016	0.0
35	4.0378752700329085E-4	0.0	0.0	53.541418505582364	0.0
36	4.0378752700329085E-4	0.0	0.0	54.19515051180069	0.0
37	4.0378752700329085E-4	0.0	0.0	54.77014395025338	0.0
38	4.0378752700329085E-4	0.0	0.0	55.33060103773394	0.0
39	4.0378752700329085E-4	0.0	0.0	55.93184066544185	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	25	3.887579E-5	45.000004	1
CGGGTAA	35	1.2100463E-7	45.000004	6
GGTACTA	50	2.1827873E-11	45.000004	8
CTCACGT	25	3.887579E-5	45.000004	45
CTCACGA	25	3.887579E-5	45.000004	24
GGTACGA	30	2.1629912E-6	45.000004	8
AGTCCCA	25	3.887579E-5	45.000004	38
AGTCATA	25	3.887579E-5	45.000004	37
GCGAAGT	35	1.2100463E-7	45.000004	27
GCGCGAC	25	3.887579E-5	45.000004	9
TAGGTGA	25	3.887579E-5	45.000004	36
CGTGAGA	30	2.1629912E-6	45.000004	2
GACCGTT	25	3.887579E-5	45.000004	20
CTATACG	25	3.887579E-5	45.000004	1
TAGAACG	25	3.887579E-5	45.000004	1
AGCCACG	25	3.887579E-5	45.000004	29
GATCCAA	30	2.1629912E-6	45.000004	29
GCGCCCC	30	2.1629912E-6	45.000004	10
ATTAACG	25	3.887579E-5	45.000004	1
GGTCATA	30	2.1629912E-6	45.000004	8
>>END_MODULE