##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934789.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	631499
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91940129754758	33.0	31.0	34.0	30.0	34.0
2	32.09396847817653	33.0	31.0	34.0	30.0	34.0
3	32.10954728352697	33.0	31.0	34.0	30.0	34.0
4	35.730721663850616	37.0	35.0	37.0	33.0	37.0
5	35.60537704731124	37.0	35.0	37.0	33.0	37.0
6	35.52052180605195	37.0	35.0	37.0	33.0	37.0
7	35.89973064090363	37.0	35.0	37.0	35.0	37.0
8	35.6979092603472	37.0	35.0	37.0	35.0	37.0
9	37.321454190742976	39.0	37.0	39.0	34.0	39.0
10	37.164606753138166	39.0	37.0	39.0	34.0	39.0
11	37.03263504771979	39.0	37.0	39.0	33.0	39.0
12	36.88375120150626	39.0	35.0	39.0	33.0	39.0
13	36.67891952322965	39.0	35.0	39.0	33.0	39.0
14	37.66642702522094	39.0	36.0	41.0	33.0	41.0
15	37.78912555681006	39.0	36.0	41.0	33.0	41.0
16	37.911532718183246	40.0	36.0	41.0	33.0	41.0
17	37.90724767576829	40.0	36.0	41.0	33.0	41.0
18	37.90403310219018	39.0	36.0	41.0	33.0	41.0
19	37.891296739979005	39.0	36.0	41.0	34.0	41.0
20	37.81320477150399	39.0	36.0	41.0	34.0	41.0
21	37.73222285387625	39.0	35.0	41.0	33.0	41.0
22	37.65748956055354	39.0	35.0	41.0	33.0	41.0
23	37.48521692037517	39.0	35.0	41.0	33.0	41.0
24	37.21637405601592	39.0	35.0	41.0	32.0	41.0
25	37.14297726520549	39.0	35.0	40.0	32.0	41.0
26	37.32640590088029	39.0	35.0	40.0	33.0	41.0
27	37.412868428928626	39.0	35.0	41.0	33.0	41.0
28	37.394711630580574	39.0	35.0	41.0	33.0	41.0
29	37.40775519834553	39.0	35.0	41.0	33.0	41.0
30	37.1752876884999	39.0	35.0	41.0	32.0	41.0
31	36.98021532892372	39.0	35.0	41.0	32.0	41.0
32	36.833733703457966	39.0	35.0	40.0	31.0	41.0
33	36.591676313026625	39.0	35.0	40.0	31.0	41.0
34	36.51646479250165	39.0	35.0	40.0	31.0	41.0
35	36.43915192264754	39.0	35.0	40.0	31.0	41.0
36	36.30335915021243	39.0	35.0	40.0	30.0	41.0
37	36.150996280279145	38.0	35.0	40.0	30.0	41.0
38	35.98558509197956	38.0	35.0	40.0	30.0	41.0
39	35.90458892254778	38.0	35.0	40.0	29.0	41.0
40	35.6959124242477	38.0	35.0	40.0	29.0	41.0
41	35.569788709087426	38.0	35.0	40.0	29.0	41.0
42	35.386076620865595	37.0	34.0	40.0	28.0	41.0
43	35.1044673071533	37.0	34.0	40.0	28.0	41.0
44	34.933146370778104	37.0	34.0	40.0	28.0	41.0
45	34.770474695921926	36.0	34.0	40.0	27.0	41.0
46	34.55180134885408	36.0	33.0	39.0	27.0	41.0
47	34.23282697201421	35.0	33.0	39.0	26.0	41.0
48	34.18831542092703	35.0	33.0	39.0	27.0	41.0
49	34.12921318956958	35.0	33.0	39.0	27.0	41.0
50	33.891939654694625	35.0	33.0	39.0	26.0	41.0
51	32.97030399098019	35.0	32.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	13.0
11	12.0
12	9.0
13	12.0
14	10.0
15	25.0
16	46.0
17	82.0
18	172.0
19	326.0
20	595.0
21	960.0
22	1445.0
23	2073.0
24	3108.0
25	4355.0
26	5829.0
27	7063.0
28	8057.0
29	9470.0
30	11668.0
31	15001.0
32	20015.0
33	28320.0
34	48727.0
35	69179.0
36	66681.0
37	104117.0
38	117034.0
39	107022.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.650422249283054	22.107081721427903	22.59766048719	13.644835542099038
2	32.96489780664736	22.734794512738738	25.035352391690246	19.26495528892366
3	31.089518748248217	23.217772316345712	28.724511044356365	16.96819789104971
4	28.830291101015206	25.49410212842776	25.959661060429234	19.71594571012781
5	22.807320359968898	28.178033536078438	28.420947618286014	20.593698485666646
6	24.26955545456129	31.89664591709567	27.505190031971548	16.32860859637149
7	73.14928448026046	6.2801366272947385	13.349031431562045	7.221547460882757
8	69.78490860634776	5.388607107849736	14.597331112163282	10.22915317363923
9	63.657741342424934	8.022182141222709	17.830115328765366	10.489961187586996
10	37.11898197780202	24.335905520040413	23.787369417845476	14.75774308431209
11	27.219203830884926	24.368684669334392	27.490621521174223	20.92148997860646
12	27.15253705864934	20.004465565266134	33.27257842055174	19.570418955532787
13	22.149362073415794	25.923398136814154	33.306307690115105	18.62093209965495
14	15.480942962696695	30.84454607212363	30.775028939079874	22.899482026099804
15	13.622666069146586	26.000357878634805	40.524213023298536	19.85276302892008
16	15.10184497520978	25.923714843570618	36.35175986026898	22.62268032095063
17	14.989572430043435	27.195767530906622	29.14826468450465	28.666395354545298
18	17.326234879231798	25.582304960102864	34.96870145479248	22.12275870587285
19	19.065746739108057	27.466551807682986	29.80812321159653	23.659578241612415
20	22.109773728857846	26.87383511296138	30.819526238363004	20.196864919817767
21	19.7029607331128	27.673678026410176	32.08888691826907	20.534474322207952
22	17.037398317337	26.125457047437923	28.5558646965395	28.28127993868557
23	17.614754734370123	26.78119838669578	29.789437512965183	25.81460936596891
24	21.702805546802132	22.99291051925656	30.933540670689897	24.370743263251406
25	17.65117601136344	26.566312852435235	27.972649204511807	27.80986193168952
26	19.16550936739409	27.931952386306236	28.752222885546928	24.15031536075275
27	23.901067143415904	26.28555231283027	27.74541210674918	22.06796843700465
28	17.75363064707941	24.850395645915512	30.674316190524454	26.72165751648063
29	24.421891404420276	22.26812710708964	28.756498426759187	24.553483061730898
30	26.08048468802009	26.317539695233087	24.818724970269155	22.78325064647767
31	22.08649578225777	29.919445636493485	21.730517387992695	26.263541193256046
32	26.387848595168006	31.041854381400448	20.93732531642964	21.63297170700191
33	22.839624449128184	32.66751016232805	22.03265563365896	22.460209754884804
34	20.713096932853418	29.448819396388593	24.874465359406745	24.963618311351247
35	23.72149441250105	28.53353687020882	23.469395834356032	24.2755728829341
36	24.431234253735955	30.72720621885387	23.938280187300375	20.9032793401098
37	21.59322500906573	29.774077235276696	27.57439045825884	21.05830729739873
38	19.775961640477657	31.940984863000576	23.39576151347825	24.887291983043518
39	22.322283962444914	30.068931225544297	26.032186907659394	21.57659790435139
40	24.101542520257354	27.633139561582837	28.276054277203922	19.989263640955883
41	19.430592922554112	30.446445679248896	24.154432548586772	25.968528849610212
42	23.81159748471494	26.188956752108872	26.109780062992975	23.889665700183215
43	26.431079067425284	24.13986403778945	25.78198857005316	23.647068324732107
44	22.012544754623523	24.478581913827256	25.83852072608191	27.670352605467308
45	22.989110038179	23.983410900096438	24.922921493145676	28.10455756857889
46	25.20336532599418	26.42680352621303	26.014609682675665	22.355221465117125
47	18.88237352711564	26.552852815285533	30.74905898504986	23.815714672548964
48	19.44436966646028	24.24390220728774	28.737654374749606	27.574073751502375
49	21.78008199537925	21.746352725815875	32.59245066104618	23.881114617758698
50	21.534950965876433	21.68839538938304	30.751275932345102	26.025377712395425
51	18.616339851686227	21.923233449300792	26.247547502054637	33.21287919695835
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	948.0
1	1134.5
2	1321.0
3	3535.0
4	5749.0
5	3673.0
6	1597.0
7	1595.5
8	1594.0
9	1709.0
10	1824.0
11	1877.0
12	1930.0
13	1961.0
14	1992.0
15	2036.5
16	2081.0
17	2049.0
18	2017.0
19	1977.0
20	1937.0
21	1839.0
22	1741.0
23	2008.5
24	2276.0
25	2312.0
26	3392.0
27	4436.0
28	4711.0
29	4986.0
30	5178.0
31	5370.0
32	6801.0
33	8232.0
34	9823.0
35	11414.0
36	12727.5
37	14041.0
38	14690.5
39	15340.0
40	18472.0
41	21604.0
42	26594.0
43	31584.0
44	40623.5
45	49663.0
46	60097.0
47	70531.0
48	82672.5
49	94814.0
50	93237.5
51	91661.0
52	72501.5
53	53342.0
54	43885.5
55	34429.0
56	29303.5
57	24178.0
58	21287.5
59	18397.0
60	17121.5
61	15846.0
62	14181.5
63	12517.0
64	10367.5
65	8218.0
66	6514.0
67	4810.0
68	3780.5
69	2751.0
70	2272.5
71	1794.0
72	1424.0
73	1054.0
74	773.5
75	364.5
76	236.0
77	281.5
78	327.0
79	175.0
80	23.0
81	15.5
82	8.0
83	10.5
84	13.0
85	10.0
86	7.0
87	12.5
88	18.0
89	10.5
90	3.0
91	3.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	631499.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.1666036443983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.17114762803148	16.401364414052367
2	12.445868744017986	6.264404913818118
3	6.1124697457647565	4.61490310140113
4	3.6646099175030353	3.6890314122052015
5	2.348938604043412	2.955740341649339
6	1.5984488957751466	2.4136517883479547
7	1.1795959287630584	2.078049623980805
8	0.8732850816185838	1.7582095614128737
9	0.7009189632183925	1.5875774760743886
>10	4.554364879199752	23.65286504460737
>50	1.1359999901776134	19.788859314274376
>100	0.2009943991579652	8.61151456064016
>500	0.006996640477017776	1.2037583786459096
>1k	0.0050884658014674735	2.861807485828667
>5k	0.0012721164503668684	2.1182625830613464
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	7129	1.128901233414463	No Hit
CCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	6104	0.9665890207268737	No Hit
CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT	4575	0.7244667054104599	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3723	0.5895496271569709	No Hit
TCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	2341	0.37070525844063096	No Hit
ACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	2028	0.3211406510540793	No Hit
CGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTG	1815	0.28741138149070705	No Hit
GCCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTG	1146	0.1814729714536365	No Hit
ACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTG	1143	0.1809979113189411	No Hit
AGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTG	1107	0.1752971897025965	No Hit
GGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTG	954	0.15106912283313195	No Hit
AAAACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC	822	0.13016647690653507	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCC	813	0.12874129650244892	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC	760	0.12034856745616383	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	734	0.11623137962213717	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.583533782317945E-4	0.0	0.0	0.7784652073875018	0.0
2	1.583533782317945E-4	0.0	0.0	3.7810036120405575	0.0
3	1.583533782317945E-4	0.0	0.0	5.27870986335687	0.0
4	1.583533782317945E-4	0.0	0.0	6.73207716876828	0.0
5	1.583533782317945E-4	0.0	0.0	10.615377063146576	0.0
6	1.583533782317945E-4	0.0	0.0	12.391468553394384	0.0
7	1.583533782317945E-4	0.0	0.0	14.268589498954077	0.0
8	1.583533782317945E-4	0.0	0.0	17.408261929155866	0.0
9	1.583533782317945E-4	0.0	0.0	18.449752097786376	0.0
10	1.583533782317945E-4	0.0	0.0	20.705812677454755	0.0
11	1.583533782317945E-4	0.0	0.0	24.009064147369987	0.0
12	1.583533782317945E-4	0.0	0.0	26.617144286847644	0.0
13	1.583533782317945E-4	0.0	0.0	27.670669312223772	0.0
14	1.583533782317945E-4	0.0	0.0	28.056259788218192	0.0
15	1.583533782317945E-4	0.0	0.0	28.849610213159483	0.0
16	1.583533782317945E-4	0.0	0.0	30.403848620504547	0.0
17	1.583533782317945E-4	0.0	0.0	32.244389935692695	0.0
18	1.583533782317945E-4	0.0	0.0	34.45136096810921	0.0
19	1.583533782317945E-4	0.0	0.0	35.66545631901238	0.0
20	1.583533782317945E-4	0.0	0.0	36.76221181664579	0.0
21	1.583533782317945E-4	0.0	0.0	38.1573050788679	0.0
22	1.583533782317945E-4	0.0	0.0	39.49412429790071	0.0
23	1.583533782317945E-4	0.0	0.0	40.77789513522587	0.0
24	1.583533782317945E-4	0.0	0.0	41.71914761543565	0.0
25	1.583533782317945E-4	0.0	0.0	42.51930723564091	0.0
26	1.583533782317945E-4	0.0	0.0	43.274969556563036	0.0
27	1.583533782317945E-4	0.0	0.0	44.06610303420908	0.0
28	1.583533782317945E-4	0.0	0.0	44.77014215382764	0.0
29	1.583533782317945E-4	0.0	0.0	45.50838560314426	0.0
30	1.583533782317945E-4	0.0	0.0	46.311553937535926	0.0
31	1.583533782317945E-4	0.0	0.0	47.093186212488064	0.0
32	1.583533782317945E-4	0.0	0.0	47.79896721926717	0.0
33	1.583533782317945E-4	0.0	0.0	48.47671967809925	0.0
34	1.583533782317945E-4	0.0	0.0	49.11884262682918	0.0
35	1.583533782317945E-4	0.0	0.0	49.8102134761892	0.0
36	1.583533782317945E-4	0.0	0.0	50.48923276204713	0.0
37	1.583533782317945E-4	0.0	0.0	51.157167311428836	0.0
38	3.16706756463589E-4	0.0	0.0	51.79121423786894	0.0
39	3.16706756463589E-4	0.0	0.0	52.4474306372615	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAATCG	30	2.1639426E-6	45.000004	7
GTACCGA	30	2.1639426E-6	45.000004	8
TACTTCA	30	2.1639426E-6	45.000004	11
ACGCACG	30	2.1639426E-6	45.000004	1
CTAACGG	60	0.0	45.000004	2
TCGTCGG	30	2.1639426E-6	45.000004	2
AACGAGC	30	2.1639426E-6	45.000004	15
AGTTACG	30	2.1639426E-6	45.000004	1
TTCGGAT	30	2.1639426E-6	45.000004	15
CGCACGG	60	0.0	45.000004	2
ATACGTA	30	2.1639426E-6	45.000004	14
TCGAAGG	30	2.1639426E-6	45.000004	2
TACGTAA	30	2.1639426E-6	45.000004	15
ACGGTGC	30	2.1639426E-6	45.000004	5
CTTGTGT	20	7.030869E-4	45.0	13
AACGTTC	20	7.030869E-4	45.0	40
AAGTACG	25	3.8888018E-5	45.0	1
AACCGAG	25	3.8888018E-5	45.0	14
GATACCC	20	7.030869E-4	45.0	14
AAATCTA	20	7.030869E-4	45.0	35
>>END_MODULE