##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934788.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1380201
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20899347268985	33.0	31.0	34.0	30.0	34.0
2	32.385432991281704	34.0	31.0	34.0	30.0	34.0
3	32.40337747907732	34.0	31.0	34.0	30.0	34.0
4	35.948771229697705	37.0	35.0	37.0	35.0	37.0
5	35.83248526845003	37.0	35.0	37.0	35.0	37.0
6	35.76653400482973	37.0	35.0	37.0	35.0	37.0
7	36.044366001763514	37.0	35.0	37.0	35.0	37.0
8	35.85358654282963	37.0	35.0	37.0	35.0	37.0
9	37.52129363766582	39.0	38.0	39.0	35.0	39.0
10	37.31310801832487	39.0	37.0	39.0	34.0	39.0
11	37.215616421086494	39.0	37.0	39.0	34.0	39.0
12	37.13109032669879	39.0	37.0	39.0	34.0	39.0
13	37.07575128550117	39.0	37.0	39.0	33.0	39.0
14	38.23126124383332	40.0	37.0	41.0	33.0	41.0
15	38.313241332240736	40.0	37.0	41.0	34.0	41.0
16	38.39501275538853	40.0	38.0	41.0	34.0	41.0
17	38.399036082425674	40.0	37.0	41.0	34.0	41.0
18	38.35554024377609	40.0	37.0	41.0	34.0	41.0
19	38.3293172516177	40.0	37.0	41.0	34.0	41.0
20	38.26790083473349	40.0	37.0	41.0	34.0	41.0
21	38.21733138868904	40.0	37.0	41.0	34.0	41.0
22	38.15972166372869	40.0	37.0	41.0	34.0	41.0
23	37.999062455395986	40.0	36.0	41.0	34.0	41.0
24	37.7616129824569	40.0	36.0	41.0	33.0	41.0
25	37.66950103644324	39.0	36.0	41.0	33.0	41.0
26	37.812187500226415	40.0	36.0	41.0	33.0	41.0
27	37.89332350867736	40.0	36.0	41.0	34.0	41.0
28	37.841378900609406	40.0	36.0	41.0	34.0	41.0
29	37.841727400574264	40.0	36.0	41.0	33.0	41.0
30	37.75077977772803	40.0	36.0	41.0	33.0	41.0
31	37.62092622741181	40.0	36.0	41.0	33.0	41.0
32	37.50615526289287	40.0	36.0	41.0	33.0	41.0
33	37.422040702767205	40.0	36.0	41.0	33.0	41.0
34	37.35715739953818	40.0	36.0	41.0	33.0	41.0
35	37.2627552073937	40.0	35.0	41.0	32.0	41.0
36	37.21089247145887	40.0	35.0	41.0	32.0	41.0
37	37.12029479764179	39.0	35.0	41.0	32.0	41.0
38	37.02477610145189	39.0	35.0	41.0	32.0	41.0
39	37.04296185845395	39.0	35.0	41.0	32.0	41.0
40	36.923872682312215	39.0	35.0	41.0	31.0	41.0
41	36.85203024776826	39.0	35.0	41.0	31.0	41.0
42	36.77052255432361	39.0	35.0	41.0	31.0	41.0
43	36.71002701780393	39.0	35.0	41.0	31.0	41.0
44	36.63980681074713	39.0	35.0	41.0	31.0	41.0
45	36.56760355919174	39.0	35.0	41.0	31.0	41.0
46	36.446007501805894	39.0	35.0	41.0	31.0	41.0
47	36.285299025286896	39.0	35.0	40.0	30.0	41.0
48	36.25142424907676	39.0	35.0	40.0	30.0	41.0
49	36.247661753614146	39.0	35.0	40.0	30.0	41.0
50	36.124558669353235	38.0	35.0	40.0	30.0	41.0
51	35.03340165671522	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	16.0
10	18.0
11	17.0
12	20.0
13	17.0
14	25.0
15	31.0
16	85.0
17	147.0
18	255.0
19	475.0
20	885.0
21	1449.0
22	2191.0
23	3298.0
24	4748.0
25	6980.0
26	9659.0
27	12028.0
28	13611.0
29	15894.0
30	19327.0
31	24174.0
32	32228.0
33	45067.0
34	81325.0
35	113414.0
36	105775.0
37	164776.0
38	298550.0
39	423408.0
40	300.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.01685044424689	21.555990757867878	23.889853724203938	11.537305073681297
2	32.886441902302636	24.0975046388171	25.00186567029005	18.014187788590213
3	31.641695666066028	23.904127007588023	26.982664119211623	17.471513207134322
4	28.201617010855667	25.72762952642405	26.811891891108612	19.25886157161167
5	24.341092348143494	30.022076494655487	25.565841496999347	20.070989660201665
6	26.633656981845398	33.46766159421708	24.39057789408934	15.508103529848189
7	78.85003705981954	5.693808365593127	9.705760247963884	5.750394326623441
8	78.83090941102057	4.844946496923274	9.808426453828103	6.515717638228055
9	72.27476287874013	7.18670686371043	12.663300490290908	7.875229767258538
10	37.83434441795072	25.845583360684422	20.689595211132293	15.630477010232568
11	27.77935967297517	25.20871959953659	26.320514185977263	20.691406541510982
12	25.18133228421078	22.544687331772693	31.614090991094773	20.659889392921755
13	22.93144259423084	25.041714938621258	32.34782470089502	19.679017766252887
14	19.20379712809946	28.625106053393672	29.941653425841597	22.22944339266527
15	17.922244658567845	26.43144005836831	34.7133497222506	20.932965560813244
16	19.22770668909818	26.596923201765538	32.063880550731376	22.111489558404898
17	20.55099221055484	25.723499693160633	28.42049817381671	25.305009922467814
18	20.889566084939805	25.87333294208597	31.837826519470713	21.399274453503512
19	21.827110688950377	26.75284252076328	29.208644248192833	22.211402542093506
20	22.50527278273237	26.980780335617784	29.280517837619303	21.233429044030544
21	22.21893767646886	26.559247529888765	31.025191258374683	20.196623535267687
22	20.80646224716545	25.501285682302793	28.327033526276246	25.365218544255512
23	19.36304929499399	26.025991866402066	30.444333832535985	24.166625006067957
24	21.177422708721412	24.774290121511285	30.92100353499237	23.127283634774933
25	19.563527341307534	27.00389291124988	28.758130156404754	24.674449591037828
26	19.400000434719292	28.178504435223566	28.82449730148	23.596997828577145
27	21.09424641773191	28.276750994963777	28.596342126980055	22.03266046032426
28	17.703798214897684	27.905790533407814	31.02540861802013	23.36500263367437
29	18.810086356987135	27.207341539384483	30.276025013747997	23.706547089880388
30	22.347397226925644	26.394054199352123	27.941220155615014	23.317328418107216
31	21.948614730752986	29.91288949942798	25.50179285480883	22.636702915010204
32	21.273857938082934	30.280154847011413	26.35246605385737	22.093521161048283
33	21.716836895495657	26.2854468298458	26.079969511687068	25.91774676297148
34	19.67423585405314	27.07344799779163	28.774504583028126	24.47781156512711
35	19.380800332705164	27.515050344116542	28.117788640929835	24.986360682248453
36	22.95491743593868	27.252190079560872	25.902531587790474	23.89036089670997
37	21.831747694719827	27.87123034978239	28.911151346796586	21.385870608701197
38	18.826098517534763	28.756825998532097	27.460130807034627	24.95694467689851
39	20.821025343410128	25.229078952993078	28.105254234709292	25.844641468887502
40	22.7601631936218	24.781028270520018	29.100544051192546	23.35826448466564
41	20.15684672015163	27.405935802104185	25.806603530934986	26.630613946809195
42	21.123300157006117	26.30109672431769	26.937960485465524	25.63764263321067
43	21.80081017185178	25.040628140394045	28.22030994036376	24.938251747390417
44	20.173293599990146	25.41825429774359	27.848769853086612	26.55968224917965
45	21.727415064907213	24.37514535926289	27.357754414031	26.5396851617989
46	22.99867917788786	25.978969729771244	27.74327797183164	23.27907312050926
47	20.056064297881253	25.963899461020535	30.138363904967463	23.841672336130753
48	19.808129395645995	24.59337444328761	29.50157259703478	26.096923564031616
49	20.916301321329282	22.892028045190518	31.315221478610724	24.876449154869473
50	20.40949108137148	22.714300308433337	30.463751294195557	26.412457315999628
51	19.784654553938157	22.891231059823895	27.70263171813381	29.621482668104136
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	682.0
1	1149.5
2	1617.0
3	4936.5
4	8256.0
5	5635.0
6	3014.0
7	2954.5
8	2895.0
9	2977.5
10	3060.0
11	3071.0
12	3082.0
13	3151.0
14	3220.0
15	3127.5
16	3035.0
17	2835.0
18	2635.0
19	2969.5
20	3304.0
21	3230.0
22	3156.0
23	4232.5
24	5309.0
25	5316.0
26	7773.5
27	10224.0
28	13104.0
29	15984.0
30	19059.5
31	22135.0
32	23569.5
33	25004.0
34	28365.5
35	31727.0
36	37146.0
37	42565.0
38	46386.0
39	50207.0
40	55790.5
41	61374.0
42	72464.5
43	83555.0
44	95214.0
45	106873.0
46	121392.5
47	135912.0
48	151649.5
49	167387.0
50	164072.5
51	160758.0
52	134701.5
53	108645.0
54	91385.5
55	74126.0
56	65910.0
57	57694.0
58	51226.5
59	44759.0
60	41069.0
61	37379.0
62	35396.5
63	33414.0
64	28168.0
65	22922.0
66	18972.0
67	15022.0
68	12230.5
69	9439.0
70	7318.5
71	5198.0
72	4495.0
73	3792.0
74	3255.5
75	2235.5
76	1752.0
77	1026.0
78	300.0
79	313.5
80	327.0
81	206.0
82	85.0
83	116.0
84	147.0
85	149.0
86	151.0
87	83.0
88	15.0
89	11.0
90	7.0
91	4.5
92	2.0
93	1.5
94	1.0
95	2.0
96	3.0
97	4.0
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1380201.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.818205427588264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.796384634112	16.14788216463068
2	12.428442784446968	5.9204640676997
3	5.623839501316843	4.018492936024503
4	3.1113278784373275	2.9642498624481055
5	1.9900608090896006	2.3699838582144315
6	1.3228723498947346	1.8905067230561525
7	0.9492072422739704	1.5825889162885176
8	0.6786462347624039	1.2931308345784185
9	0.5176963713008139	1.1097538668683815
>10	3.607819829749928	18.61187903048787
>50	1.5006902372814215	26.207479720748715
>100	0.4641105363600249	14.677846791510515
>500	0.005525125432109337	0.8802482297579319
>1k	0.0027625627160546683	1.428063843177881
>5k	6.139028257899263E-4	0.8974291545081905
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	6880	0.4984781202158236	TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	5395	0.39088509572156516	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4704	0.34081992405453987	No Hit
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	3897	0.2823501794303873	TruSeq Adapter, Index 14 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	2629	0.19047950262316865	TruSeq Adapter, Index 21 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	1868	0.13534260589580793	TruSeq Adapter, Index 14 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	1683	0.12193876109349291	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4490643029529756E-4	0.0	0.0	0.30633219364425907	0.0
2	1.4490643029529756E-4	0.0	0.0	1.6176629346015543	0.0
3	1.4490643029529756E-4	0.0	0.0	2.3382101592449214	0.0
4	1.4490643029529756E-4	0.0	0.0	3.1403397041445413	0.0
5	1.4490643029529756E-4	0.0	0.0	5.20069178329823	0.0
6	1.4490643029529756E-4	0.0	0.0	6.254233984760191	0.0
7	1.4490643029529756E-4	0.0	0.0	7.342988448783909	0.0
8	1.4490643029529756E-4	0.0	0.0	9.050276010523104	0.0
9	1.4490643029529756E-4	0.0	0.0	9.646565971188254	0.0
10	1.4490643029529756E-4	0.0	0.0	11.049694935737621	0.0
11	1.4490643029529756E-4	0.0	0.0	13.20648224425283	0.0
12	1.4490643029529756E-4	0.0	0.0	14.760168989879011	0.0
13	1.4490643029529756E-4	0.0	0.0	15.388555724854568	0.0
14	1.4490643029529756E-4	0.0	0.0	15.625912457678266	0.0
15	1.4490643029529756E-4	0.0	0.0	16.120115838200377	0.0
16	1.4490643029529756E-4	0.0	0.0	17.25705169029728	0.0
17	1.4490643029529756E-4	0.0	0.0	18.588524425065625	0.0
18	1.4490643029529756E-4	0.0	0.0	20.109824583520805	0.0
19	1.4490643029529756E-4	0.0	0.0	20.968105370159854	0.0
20	1.4490643029529756E-4	0.0	0.0	21.796752791803513	0.0
21	1.4490643029529756E-4	0.0	0.0	22.857685221210534	0.0
22	1.4490643029529756E-4	0.0	0.0	23.89934509538828	0.0
23	1.4490643029529756E-4	0.0	0.0	24.9617990423134	0.0
24	2.1735964544294635E-4	0.0	0.0	25.76929012513395	0.0
25	2.1735964544294635E-4	0.0	0.0	26.49918381453136	0.0
26	2.1735964544294635E-4	0.0	0.0	27.183866697676642	0.0
27	2.1735964544294635E-4	0.0	0.0	27.826309356390844	0.0
28	2.1735964544294635E-4	0.0	0.0	28.469983719762556	0.0
29	2.1735964544294635E-4	0.0	0.0	29.15328999182003	0.0
30	2.1735964544294635E-4	0.0	0.0	29.896949792095498	0.0
31	2.1735964544294635E-4	0.0	0.0	30.613439636690597	0.0
32	2.1735964544294635E-4	0.0	0.0	31.24943395925666	0.0
33	3.6226607573824394E-4	0.0	0.0	31.877241068511037	0.0
34	3.6226607573824394E-4	0.0	0.0	32.50954027710457	0.0
35	4.347192908858927E-4	0.0	0.0	33.19661411634972	0.0
36	4.347192908858927E-4	0.0	0.0	33.80485885751423	0.0
37	4.347192908858927E-4	0.0	0.0	34.400062019952166	0.0
38	5.071725060335415E-4	0.0	0.0	35.012726407240685	0.0
39	5.071725060335415E-4	0.0	0.0	35.609088821120984	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGTA	20	7.033778E-4	45.000004	34
CAATTCG	30	2.1658198E-6	45.000004	15
CGCGAAA	20	7.033778E-4	45.000004	37
AAACGCG	20	7.033778E-4	45.000004	1
GTCGTAG	20	7.033778E-4	45.000004	1
CTCGACG	20	7.033778E-4	45.000004	15
CCGCGAA	20	7.033778E-4	45.000004	36
TAAGCGA	20	7.033778E-4	45.000004	37
TATCGCG	20	7.033778E-4	45.000004	1
TTCGAAC	20	7.033778E-4	45.000004	36
CCTGATA	20	7.033778E-4	45.000004	30
ATACCGG	30	2.1658198E-6	45.000004	2
ATCGCAC	20	7.033778E-4	45.000004	12
TCGAACG	30	2.1658198E-6	45.000004	1
AATCGGC	20	7.033778E-4	45.000004	11
CGAAGTC	30	2.1658198E-6	45.000004	14
CGGACGA	20	7.033778E-4	45.000004	42
TAGGTCG	25	3.891215E-5	45.0	1
CAATGCG	25	3.891215E-5	45.0	45
TGTCGCG	25	3.891215E-5	45.0	1
>>END_MODULE