##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934787.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	794824
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94871191609715	33.0	31.0	34.0	30.0	34.0
2	32.12657141706843	33.0	31.0	34.0	30.0	34.0
3	32.141986150393045	34.0	31.0	34.0	30.0	34.0
4	35.76052685877628	37.0	35.0	37.0	35.0	37.0
5	35.632472094451096	37.0	35.0	37.0	33.0	37.0
6	35.53885136835324	37.0	35.0	37.0	33.0	37.0
7	35.905246947751955	37.0	35.0	37.0	35.0	37.0
8	35.63499969804636	37.0	35.0	37.0	35.0	37.0
9	37.398905921310885	39.0	37.0	39.0	35.0	39.0
10	37.18154459352007	39.0	37.0	39.0	34.0	39.0
11	37.059793111430956	39.0	37.0	39.0	33.0	39.0
12	36.841538504121665	39.0	35.0	39.0	33.0	39.0
13	36.60563973911206	39.0	35.0	39.0	33.0	39.0
14	37.594164242649946	39.0	36.0	41.0	33.0	41.0
15	37.66291405392892	39.0	36.0	41.0	33.0	41.0
16	37.884746811872816	40.0	36.0	41.0	33.0	41.0
17	37.8549389550391	39.0	36.0	41.0	33.0	41.0
18	37.84982461526074	39.0	36.0	41.0	33.0	41.0
19	37.83241824605196	39.0	36.0	41.0	34.0	41.0
20	37.71767208841202	39.0	35.0	41.0	33.0	41.0
21	37.64569011504434	39.0	35.0	41.0	33.0	41.0
22	37.585003724094896	39.0	35.0	41.0	33.0	41.0
23	37.38881060461184	39.0	35.0	41.0	33.0	41.0
24	37.09042379193381	39.0	35.0	41.0	32.0	41.0
25	37.0442890501545	39.0	35.0	40.0	32.0	41.0
26	37.27451234487132	39.0	35.0	40.0	33.0	41.0
27	37.34010044991092	39.0	35.0	41.0	33.0	41.0
28	37.219322768310974	39.0	35.0	41.0	33.0	41.0
29	37.296661902509236	39.0	35.0	41.0	33.0	41.0
30	37.194463176753594	39.0	35.0	41.0	32.0	41.0
31	37.00882207885016	39.0	35.0	41.0	32.0	41.0
32	36.93858137147343	39.0	35.0	41.0	32.0	41.0
33	36.77088638491037	39.0	35.0	40.0	31.0	41.0
34	36.69646739403944	39.0	35.0	40.0	31.0	41.0
35	36.72508505027528	39.0	35.0	41.0	31.0	41.0
36	36.563080631687015	39.0	35.0	40.0	31.0	41.0
37	36.411590490473365	39.0	35.0	40.0	30.0	41.0
38	36.27020447293992	38.0	35.0	40.0	30.0	41.0
39	36.35881025233259	39.0	35.0	40.0	30.0	41.0
40	36.29090968566626	39.0	35.0	40.0	30.0	41.0
41	36.17433419222369	38.0	35.0	40.0	30.0	41.0
42	36.05647162139039	38.0	35.0	40.0	30.0	41.0
43	35.95291536239469	38.0	35.0	40.0	30.0	41.0
44	35.830878282487696	38.0	35.0	40.0	29.0	41.0
45	35.81117706561452	38.0	35.0	40.0	29.0	41.0
46	35.73713174237315	38.0	35.0	40.0	28.0	41.0
47	35.457096162169236	38.0	34.0	40.0	28.0	41.0
48	35.427819240485945	38.0	34.0	40.0	28.0	41.0
49	35.436710517045285	38.0	34.0	40.0	28.0	41.0
50	35.310944309683656	38.0	34.0	40.0	28.0	41.0
51	34.13501227944803	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	9.0
11	23.0
12	13.0
13	5.0
14	13.0
15	20.0
16	42.0
17	86.0
18	175.0
19	385.0
20	709.0
21	1244.0
22	1806.0
23	2683.0
24	3859.0
25	5413.0
26	7103.0
27	8303.0
28	9790.0
29	11193.0
30	14120.0
31	17997.0
32	23521.0
33	32783.0
34	59011.0
35	80094.0
36	65761.0
37	99485.0
38	168196.0
39	180859.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.44990085855485	21.287479995571346	23.20161444546214	13.061004700411663
2	31.019445814419296	28.35936005958552	22.578835062856683	18.042359063138505
3	28.386913329240183	26.404713496321197	28.282487695389168	16.925885479049448
4	27.139844795829017	24.396092719897737	30.09295139552907	18.371111088744176
5	26.377160226666536	26.198001066902858	26.934264692560866	20.490574013869736
6	23.79935683874669	32.308284601370865	27.043471259046026	16.848887300836413
7	72.12665948688011	5.603253047215484	15.621949010095317	6.648138455809084
8	69.75368131812829	7.395222086902257	13.345470192143166	9.505626402826287
9	64.58536732660313	6.291581532515375	16.129734381447967	12.993316759433535
10	38.90622326452146	22.41527684116232	23.031891336950068	15.646608557366159
11	29.453187120670737	23.747144021821185	26.3450776524111	20.45459120509698
12	26.544492868861536	19.768275744064095	32.87671232876712	20.810519058307246
13	21.157765744366046	26.833739293227183	34.59658993689169	17.411905025515082
14	16.711120952563082	31.4974132638169	29.348258230752972	22.44320755286705
15	13.076605638480974	26.584627540184997	41.438985234467005	18.89978158686703
16	14.690422030537578	24.919730657353075	34.51430756997775	25.875539742131593
17	15.495883365373968	25.54880074079293	30.610047004116637	28.34526888971646
18	17.01898785139855	25.346995058025424	34.195620665707125	23.438396424868902
19	19.13794752045736	26.82631626624259	29.69009491409419	24.345641299205862
20	21.688323452739223	26.86619930953268	31.588880053949048	19.85659718377905
21	19.403289281652288	28.69868046259298	31.8927209042505	20.005309351504234
22	16.37217799160569	25.799925518102118	29.097510895493844	28.730385594798346
23	16.374820085956134	28.26625768723642	29.769483558624298	25.589438668183146
24	20.92324841725967	23.30566263726309	30.39301782532988	25.378071120147354
25	16.49182712147595	28.876329854156392	27.707266011091765	26.924577013275897
26	16.537245981500305	26.801279276921687	29.763067043773212	26.8984076978048
27	22.586132275824585	27.92995682062947	27.441798435880145	22.0421124676658
28	15.388564009139131	27.085115698569744	32.94414864171188	24.582171650579248
29	17.59383209364589	21.952155445733897	32.65351826316266	27.80049419745755
30	22.46610570390426	24.79114873229797	30.556827675057622	22.185917888740146
31	23.746263323704365	27.982672893621736	24.08407395851157	24.186989824162332
32	24.967414169677816	26.764038327982043	26.612935693939793	21.655611808400348
33	23.213818405080875	26.868715589866436	26.933383994444053	22.98408201060864
34	22.256373738085415	25.134872625889503	28.62960856743128	23.9791450685938
35	23.461294575906113	21.477584974786872	28.07124596137006	26.989874487936955
36	24.546314655823174	26.785049268768933	27.16727225146699	21.501363823940896
37	23.895856189546365	25.712358962487293	31.861519028111886	18.530265819854456
38	20.2232192284078	29.11336346159653	28.3281581834469	22.33525912654877
39	22.534422714965828	23.95146598492245	32.769267158515596	20.74484414159613
40	25.986256076817003	20.080797761517015	32.1092468269705	21.82369933469548
41	23.496396686562058	24.66810262397713	24.984776503980754	26.850724185480058
42	23.842636860487353	23.768280776624763	27.578306643986593	24.81077571890129
43	24.733022656588126	25.14380542107435	27.000191237305366	23.122980685032157
44	20.749876702263645	23.661338862439987	29.77388704920838	25.81489738608799
45	20.712761567340696	20.889027004720543	29.330770082433343	29.06744134550542
46	26.68968224411945	22.287323986190653	29.025042021881575	21.99795174780832
47	19.42014835988848	22.656839753203226	34.87551961188892	23.047492275019376
48	21.553576640866407	20.998485199239077	30.72378287520256	26.724155284691957
49	21.361458637383873	18.963946735377895	36.13353396475195	23.541060662486284
50	22.051045262850643	19.004207220718044	32.95987539379787	25.98487212263344
51	19.81017181162119	19.482174670115647	27.700723682223988	33.00692983603917
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	987.0
1	1053.0
2	1119.0
3	3535.5
4	5952.0
5	3920.0
6	1888.0
7	1954.5
8	2021.0
9	2128.0
10	2235.0
11	2202.5
12	2170.0
13	2134.5
14	2099.0
15	2075.5
16	2052.0
17	2025.5
18	1999.0
19	2115.0
20	2231.0
21	2103.5
22	1976.0
23	2069.0
24	2162.0
25	2410.5
26	3853.5
27	5048.0
28	5754.0
29	6460.0
30	6944.5
31	7429.0
32	9095.5
33	10762.0
34	11460.0
35	12158.0
36	14799.0
37	17440.0
38	19016.5
39	20593.0
40	24850.0
41	29107.0
42	37501.5
43	45896.0
44	54841.5
45	63787.0
46	88106.0
47	112425.0
48	116261.0
49	120097.0
50	116060.0
51	112023.0
52	87869.5
53	63716.0
54	52324.5
55	40933.0
56	33538.0
57	26143.0
58	23707.5
59	21272.0
60	17904.0
61	14536.0
62	13653.5
63	12771.0
64	10362.0
65	7953.0
66	6481.5
67	5010.0
68	4058.5
69	3107.0
70	2480.5
71	1854.0
72	1566.0
73	1278.0
74	931.0
75	578.0
76	572.0
77	386.0
78	200.0
79	148.5
80	97.0
81	54.5
82	12.0
83	10.0
84	8.0
85	5.0
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	794824.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.998025273729183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.00893376497287	14.950771017450323
2	12.310092610737298	5.662156419673665
3	6.216236162756267	4.288834691356138
4	3.6197859989145718	3.3299171955411344
5	2.398474357877837	2.7580086950432934
6	1.6440688430289154	2.2686202082237816
7	1.1911905431508645	1.9176521152047543
8	0.9111363331835848	1.6763469134695212
9	0.6903150273400588	1.428829420104155
>10	4.45609289529199	20.588323543214052
>50	1.1568262388925674	19.11828078553542
>100	0.3808185755360093	12.676837214837816
>500	0.005527120109376042	0.9722861740947412
>1k	0.007737968153126459	3.391879235134537
>5k	0.002763560054688021	4.971256371116647
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	9117	1.1470463901442332	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	8040	1.0115446941712882	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGC	7849	0.9875142169838859	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCG	7292	0.9174358096886859	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTC	6811	0.8569192676617716	No Hit
CTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGCT	4741	0.5964842531176714	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3901	0.4908004790997756	No Hit
TCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	2910	0.36611878856199614	No Hit
ACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	2195	0.27616176663009673	No Hit
CGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG	1877	0.23615290932332192	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCT	1648	0.20734149950177647	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGATTGGAT	1540	0.1937535856994756	No Hit
ACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG	1273	0.1601612432437873	No Hit
AGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG	1234	0.15525449659295643	No Hit
GCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG	1209	0.15210914617575713	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTC	1071	0.13474681187281712	No Hit
GGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG	1036	0.13034332128873816	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGATTGGATCGTA	1030	0.1295884371886103	No Hit
GAACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCT	1019	0.1282044830050426	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCC	934	0.11751029158656508	No Hit
AAAACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTC	890	0.11197447485229435	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	858	0.10794842631827928	No Hit
GACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCT	844	0.10618703008464768	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6393868328082695	0.0
2	0.0	0.0	0.0	3.647222529767596	0.0
3	0.0	0.0	0.0	4.934048292452165	0.0
4	0.0	0.0	0.0	6.445577888941451	0.0
5	0.0	0.0	0.0	10.75068191197045	0.0
6	1.2581401668797118E-4	0.0	0.0	12.42803438245448	0.0
7	1.2581401668797118E-4	0.0	0.0	14.39815606977142	0.0
8	1.2581401668797118E-4	0.0	0.0	17.14794721850372	0.0
9	1.2581401668797118E-4	0.0	0.0	18.044120459372138	0.0
10	1.2581401668797118E-4	0.0	0.0	20.93696214507866	0.0
11	1.2581401668797118E-4	0.0	0.0	23.95247249705595	0.0
12	1.2581401668797118E-4	0.0	0.0	27.312084184674845	0.0
13	1.2581401668797118E-4	0.0	0.0	28.26298652280253	0.0
14	1.2581401668797118E-4	0.0	0.0	28.626966473080834	0.0
15	1.2581401668797118E-4	0.0	0.0	29.406887562529565	0.0
16	1.2581401668797118E-4	0.0	0.0	30.86708504021016	0.0
17	1.2581401668797118E-4	0.0	0.0	32.57501031674937	0.0
18	1.2581401668797118E-4	0.0	0.0	34.60074179944239	0.0
19	1.2581401668797118E-4	1.2581401668797118E-4	0.0	35.85623987197166	0.0
20	1.2581401668797118E-4	1.2581401668797118E-4	0.0	36.85797107284128	0.0
21	1.2581401668797118E-4	1.2581401668797118E-4	0.0	38.06452749287892	0.0
22	2.5162803337594236E-4	1.2581401668797118E-4	0.0	39.21245458113997	0.0
23	3.7744205006391354E-4	1.2581401668797118E-4	0.0	40.41183960222641	0.0
24	3.7744205006391354E-4	1.2581401668797118E-4	0.0	41.283856551890736	0.0
25	3.7744205006391354E-4	1.2581401668797118E-4	0.0	42.01584250098135	0.0
26	3.7744205006391354E-4	1.2581401668797118E-4	0.0	42.71989773836724	0.0
27	3.7744205006391354E-4	1.2581401668797118E-4	0.0	43.383063420329535	0.0
28	3.7744205006391354E-4	1.2581401668797118E-4	0.0	44.04434189204151	0.0
29	3.7744205006391354E-4	1.2581401668797118E-4	0.0	44.74990689762765	0.0
30	3.7744205006391354E-4	1.2581401668797118E-4	0.0	45.54681287932926	0.0
31	3.7744205006391354E-4	1.2581401668797118E-4	0.0	46.301319537407025	0.0
32	3.7744205006391354E-4	1.2581401668797118E-4	0.0	46.963478707235815	0.0
33	3.7744205006391354E-4	1.2581401668797118E-4	0.0	47.54901714090163	0.0
34	3.7744205006391354E-4	1.2581401668797118E-4	0.0	48.174690245890915	0.0
35	3.7744205006391354E-4	1.2581401668797118E-4	0.0	48.877864785159986	0.0
36	3.7744205006391354E-4	1.2581401668797118E-4	0.0	49.520271154368764	0.0
37	3.7744205006391354E-4	1.2581401668797118E-4	0.0	50.13449518383944	0.0
38	3.7744205006391354E-4	1.2581401668797118E-4	0.0	50.71424617273761	0.0
39	3.7744205006391354E-4	1.2581401668797118E-4	0.0	51.3000362344368	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	30	2.164652E-6	45.000004	1
CGGGTGT	60	0.0	45.000004	6
CGCATTG	30	2.164652E-6	45.000004	2
TTCGTTG	30	2.164652E-6	45.000004	31
TCGATAG	30	2.164652E-6	45.000004	1
TCGATAC	30	2.164652E-6	45.000004	24
TGCGAAT	30	2.164652E-6	45.000004	27
TCTCCGA	30	2.164652E-6	45.000004	20
CTCGATA	30	2.164652E-6	45.000004	23
TATAGCG	60	0.0	45.000004	1
ATGCGAA	30	2.164652E-6	45.000004	26
CCGCGTT	30	2.164652E-6	45.000004	1
TGCGTAG	30	2.164652E-6	45.000004	1
TCGCCCC	20	7.0319726E-4	45.0	10
ACAACGT	20	7.0319726E-4	45.0	16
CTTAACG	40	6.8102963E-9	45.0	1
AACGTGT	20	7.0319726E-4	45.0	16
TCGTTTA	20	7.0319726E-4	45.0	17
GTCGCTC	25	3.889716E-5	45.0	16
TCGTTGT	20	7.0319726E-4	45.0	32
>>END_MODULE