SRR2934783.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934783.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2161005
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	13532	0.6261901291297336	TruSeq Adapter, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	11696	0.5412296593483125	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	8953	0.41429797709861843	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	6074	0.2810729267169673	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5313	0.24585783003741313	No Hit
ACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	4172	0.19305832240091994	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	3600	0.1665891564341591	No Hit
AGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	2855	0.1321144560054234	No Hit
ACCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	2823	0.1306336635037864	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	2594	0.12003674216394687	TruSeq Adapter, Index 27 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	2298	0.1063394115238049	TruSeq Adapter, Index 27 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCGT	25	3.891949E-5	45.000004	29
CGACGGT	30	2.1663927E-6	45.000004	28
CGCGCCA	30	2.1663927E-6	45.000004	39
GTATCCG	25	3.891949E-5	45.000004	1
CGACTCG	25	3.891949E-5	45.000004	2
GCGTAAC	25	3.891949E-5	45.000004	32
ACGCGCC	30	2.1663927E-6	45.000004	38
TATTTCG	20	7.0346677E-4	45.0	12
CGGCGGT	20	7.0346677E-4	45.0	30
ACCGGTA	20	7.0346677E-4	45.0	36
GTCGATG	35	1.2125929E-7	45.0	1
GGCCGTA	20	7.0346677E-4	45.0	8
ATTCGTT	35	1.2125929E-7	45.0	25
CGCGATA	20	7.0346677E-4	45.0	11
CCGTCGC	20	7.0346677E-4	45.0	19
CGTTATA	20	7.0346677E-4	45.0	4
GCCGACG	40	6.8193913E-9	45.0	1
GTTCGAC	20	7.0346677E-4	45.0	17
TCCGTCG	20	7.0346677E-4	45.0	18
CCGCGAT	20	7.0346677E-4	45.0	10