##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934783.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2161005
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03675188164766	33.0	31.0	34.0	30.0	34.0
2	32.21623411329451	34.0	31.0	34.0	30.0	34.0
3	32.21235536243553	34.0	31.0	34.0	30.0	34.0
4	35.81783984766347	37.0	35.0	37.0	35.0	37.0
5	35.697314906721644	37.0	35.0	37.0	33.0	37.0
6	35.62239143361538	37.0	35.0	37.0	33.0	37.0
7	36.01212769058841	37.0	35.0	37.0	35.0	37.0
8	35.878125224143396	37.0	35.0	37.0	35.0	37.0
9	37.613835229441854	39.0	38.0	39.0	35.0	39.0
10	37.13371602564548	39.0	37.0	39.0	34.0	39.0
11	37.0742446222938	39.0	37.0	39.0	33.0	39.0
12	37.08033067947552	39.0	37.0	39.0	33.0	39.0
13	37.06213682985463	39.0	37.0	39.0	33.0	39.0
14	38.17117406021735	40.0	37.0	41.0	33.0	41.0
15	38.20886300586995	40.0	37.0	41.0	33.0	41.0
16	38.29498682326047	40.0	37.0	41.0	34.0	41.0
17	38.30742825676016	40.0	37.0	41.0	34.0	41.0
18	38.28076473677756	40.0	37.0	41.0	34.0	41.0
19	38.24157510047409	40.0	37.0	41.0	34.0	41.0
20	38.19584267505165	40.0	37.0	41.0	34.0	41.0
21	38.16010513626762	40.0	37.0	41.0	34.0	41.0
22	38.11454346473053	40.0	37.0	41.0	34.0	41.0
23	37.919025638533924	40.0	37.0	41.0	33.0	41.0
24	37.650560271725425	39.0	36.0	41.0	33.0	41.0
25	37.606469674989185	39.0	36.0	41.0	33.0	41.0
26	37.81714618892599	39.0	37.0	41.0	33.0	41.0
27	37.93891684655982	40.0	37.0	41.0	34.0	41.0
28	37.92254252072531	40.0	37.0	41.0	34.0	41.0
29	37.90211545091289	40.0	37.0	41.0	34.0	41.0
30	37.7910444445987	40.0	37.0	41.0	33.0	41.0
31	37.719162611840325	40.0	37.0	41.0	33.0	41.0
32	37.60222812996731	40.0	36.0	41.0	33.0	41.0
33	37.52450966101421	40.0	36.0	41.0	33.0	41.0
34	37.50564297630038	40.0	37.0	41.0	33.0	41.0
35	37.427824091105755	40.0	36.0	41.0	33.0	41.0
36	37.41122579540538	40.0	36.0	41.0	33.0	41.0
37	37.32074196959285	40.0	36.0	41.0	32.0	41.0
38	37.20315779000974	40.0	36.0	41.0	32.0	41.0
39	37.24896286681428	40.0	36.0	41.0	32.0	41.0
40	37.230036024905075	40.0	36.0	41.0	32.0	41.0
41	37.19668024831039	40.0	36.0	41.0	32.0	41.0
42	37.116121434240085	39.0	36.0	41.0	32.0	41.0
43	37.05051445970741	39.0	36.0	41.0	31.0	41.0
44	36.990547453615335	39.0	36.0	41.0	31.0	41.0
45	36.93299321380562	39.0	35.0	41.0	31.0	41.0
46	36.89034268777721	39.0	35.0	41.0	31.0	41.0
47	36.765885779995884	39.0	35.0	41.0	31.0	41.0
48	36.73603577964882	39.0	35.0	41.0	31.0	41.0
49	36.74117829435841	39.0	35.0	41.0	31.0	41.0
50	36.6427703776715	39.0	35.0	41.0	31.0	41.0
51	35.598464140527206	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	16.0
10	30.0
11	19.0
12	29.0
13	19.0
14	27.0
15	43.0
16	65.0
17	151.0
18	349.0
19	590.0
20	1114.0
21	1818.0
22	2937.0
23	4546.0
24	6992.0
25	11007.0
26	15243.0
27	17586.0
28	19437.0
29	22974.0
30	28626.0
31	37858.0
32	50420.0
33	70277.0
34	115959.0
35	152845.0
36	167954.0
37	268669.0
38	509184.0
39	653886.0
40	330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.772649762494765	22.591618251693077	26.255422824102677	10.380309161709482
2	33.531157956598896	24.190041207678835	24.787263333495297	17.491537502226972
3	30.54935088072448	24.320536046885593	28.05713082570378	17.07298224668615
4	27.791050923065892	26.384483145573473	27.634873588908864	18.18959234245178
5	24.519471264527386	29.40742848813399	27.245332611446987	18.827767635891636
6	24.2337708612428	33.331713716534665	27.264120166311507	15.170395255911023
7	78.92341757654424	5.370325381014852	10.308583274911442	5.39767376752946
8	79.17121894674007	3.850569526678559	10.09701504624006	6.881196480341323
9	74.37303476854518	5.591379936649846	12.667022982362372	7.368562312442591
10	35.9284684672178	21.712860451502888	24.65265004014336	17.70602104113595
11	25.762827943479998	27.235707460186347	25.509843799528458	21.49162079680519
12	22.336042720863674	22.908554121809065	33.04203368340193	21.713369473925326
13	22.70101179775151	25.092630512192244	34.07895863267322	18.127399057383023
14	20.576676129856246	27.585498413932406	30.959391579380892	20.878433876830456
15	18.8693686502345	26.858799493754066	34.957022311378275	19.314809544633167
16	20.059277974831154	26.644593603439144	31.369293453740273	21.926834967989432
17	20.64696749891833	26.749822420586717	28.6427842601012	23.960425820393752
18	20.766032471003072	26.217477516248227	32.75637955488303	20.260110457865668
19	20.179916288948892	28.706921085328354	29.932045506604567	21.181117119118188
20	20.084543996890336	29.130612839859232	30.54028102665195	20.244562136598482
21	20.016751465174767	28.23755613707511	31.91422509434268	19.831467303407443
22	18.87677261274268	26.03557141237526	29.06189481283014	26.025761162051914
23	17.723003880139103	27.639084592585395	30.046668101184405	24.5912434260911
24	20.452613483078473	25.939042251174797	30.30113303763758	23.307211228109143
25	17.975479001668205	28.07425248900396	29.137415230413627	24.81285327891421
26	17.476081730491135	27.759537807640427	31.99400279036837	22.770377671500068
27	20.3384536361554	26.4855472338102	29.76749243986016	23.408506690174246
28	18.149657219673255	25.975784415121666	31.067628256297418	24.80693010890766
29	18.793061561634516	25.22516144108875	31.56489688825338	24.41688010902335
30	20.110365316137628	25.916737814118896	30.07276706902575	23.90012980071772
31	20.159324018223003	27.73353138933043	28.71816585338766	23.38897873905891
32	20.068347828903683	28.27800028227607	28.40488568976009	23.24876619906016
33	19.55261556544293	25.231963831643146	29.197387326729924	26.018033276184
34	18.685009983780695	23.98485889667076	32.89691601824151	24.43321510130703
35	18.950118116339386	24.17949056110467	31.239492736018658	25.63089858653728
36	20.360341600320222	23.840481627761157	30.4883607395633	25.310816032355316
37	20.179638640354835	25.37125087632838	31.417974507231587	23.031135976085203
38	20.560202313275536	27.36569327697067	28.078509767446164	23.99559464230763
39	19.72508161711796	24.232984190226308	32.63185416044849	23.410080032207237
40	20.78102549508215	22.14636245635711	34.76692557398062	22.305686474580114
41	20.289680033132733	22.947702573571092	29.62339281954461	27.139224573751562
42	21.563207859306203	22.29707936816435	29.593962068574577	26.545750703954873
43	21.648260878618974	23.195503943766905	29.76337398571498	25.39286119189914
44	20.264645384901932	23.895224675556047	28.879202037940683	26.960927901601337
45	20.469087299659186	22.721141320820635	30.235978167565552	26.573793211954623
46	22.38879595373449	22.268712936804867	31.75753873776322	23.58495237169743
47	19.1037966131499	22.559873762439235	33.72199509024736	24.614334534163504
48	19.57913100617537	21.472185395221207	31.5376873260358	27.410996272567623
49	21.27940472141434	20.1882457467706	33.154759012589054	25.377590519226008
50	20.14974514172804	19.35136660951733	32.988354955217595	27.510533293537037
51	19.53156054706028	19.486026177634944	29.471611588126823	31.51080168717796
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	699.0
1	966.0
2	1233.0
3	5469.5
4	9706.0
5	6946.0
6	4186.0
7	4127.5
8	4069.0
9	4185.0
10	4301.0
11	4387.0
12	4473.0
13	4411.5
14	4350.0
15	4287.5
16	4225.0
17	4477.5
18	4730.0
19	4720.5
20	4711.0
21	4774.0
22	4837.0
23	5586.0
24	6335.0
25	7622.0
26	10586.5
27	12264.0
28	14263.5
29	16263.0
30	20302.0
31	24341.0
32	30424.0
33	36507.0
34	42026.0
35	47545.0
36	54321.0
37	61097.0
38	69977.0
39	78857.0
40	91279.5
41	103702.0
42	118179.0
43	132656.0
44	154579.0
45	176502.0
46	214640.0
47	252778.0
48	291601.0
49	330424.0
50	319908.5
51	309393.0
52	250955.5
53	192518.0
54	156166.0
55	119814.0
56	96578.5
57	73343.0
58	60463.5
59	47584.0
60	39857.5
61	32131.0
62	26745.5
63	21360.0
64	16775.0
65	12190.0
66	9411.5
67	6633.0
68	4661.5
69	2690.0
70	2241.5
71	1793.0
72	1291.5
73	790.0
74	700.0
75	395.5
76	181.0
77	164.5
78	148.0
79	112.5
80	77.0
81	52.0
82	27.0
83	17.5
84	8.0
85	6.0
86	4.0
87	4.0
88	4.0
89	5.0
90	6.0
91	3.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2161005.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.049738115725308
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.30879540135885	16.491775406274325
2	13.70048720081497	7.137882072781529
3	6.6190223909919395	5.17271399602386
4	3.768311282867444	3.9265408822891916
5	2.402684976570035	3.12946572071185
6	1.637559860169397	2.559480330374193
7	1.1445225324113646	2.0870158565803947
8	0.8878213524584965	1.8502010980074308
9	0.6820376258153493	1.599021138380481
>10	4.728419234332402	26.219094448828674
>50	0.9244110516301757	16.486326553893548
>100	0.17985611985721167	7.357063654851194
>500	0.011195507694232368	1.964148654305246
>1k	0.003972599504405034	1.8758099443525382
>5k	5.417181142370501E-4	0.956767497570857
>10k+	3.611454094913667E-4	1.1866927447747089
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	13532	0.6261901291297336	TruSeq Adapter, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	11696	0.5412296593483125	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	8953	0.41429797709861843	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	6074	0.2810729267169673	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5313	0.24585783003741313	No Hit
ACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	4172	0.19305832240091994	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	3600	0.1665891564341591	No Hit
AGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	2855	0.1321144560054234	No Hit
ACCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	2823	0.1306336635037864	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	2594	0.12003674216394687	TruSeq Adapter, Index 27 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	2298	0.1063394115238049	TruSeq Adapter, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8509906270462124E-4	0.0	0.0	0.4349365225901837	0.0
2	1.8509906270462124E-4	0.0	0.0	2.1659829570038016	0.0
3	1.8509906270462124E-4	0.0	0.0	3.160427671384379	0.0
4	1.8509906270462124E-4	0.0	0.0	4.257833739394402	0.0
5	1.8509906270462124E-4	0.0	0.0	7.012015242907814	0.0
6	1.8509906270462124E-4	0.0	0.0	8.519184360980192	0.0
7	1.8509906270462124E-4	0.0	0.0	10.024965236082286	0.0
8	1.8509906270462124E-4	0.0	0.0	12.354575764516973	0.0
9	1.8509906270462124E-4	0.0	0.0	13.074611118437948	0.0
10	1.8509906270462124E-4	0.0	0.0	15.06558291165453	0.0
11	1.8509906270462124E-4	0.0	0.0	18.06872265450566	0.0
12	1.8509906270462124E-4	0.0	0.0	20.149051020242897	0.0
13	1.8509906270462124E-4	0.0	0.0	20.99282509758191	0.0
14	1.8509906270462124E-4	0.0	0.0	21.283708274622224	0.0
15	1.8509906270462124E-4	0.0	0.0	21.90397523374541	0.0
16	1.8509906270462124E-4	0.0	0.0	23.382315172801544	0.0
17	1.8509906270462124E-4	0.0	0.0	25.234277569926956	0.0
18	1.8509906270462124E-4	0.0	0.0	27.2117371315661	0.0
19	1.8509906270462124E-4	0.0	0.0	28.346995957899217	0.0
20	1.8509906270462124E-4	0.0	0.0	29.411870865638903	0.0
21	1.8509906270462124E-4	0.0	0.0	30.80339934428657	0.0
22	2.3137382838077654E-4	0.0	0.0	32.29839819898612	0.0
23	2.3137382838077654E-4	0.0	0.0	33.84975971827922	0.0
24	2.3137382838077654E-4	0.0	0.0	34.97270945694249	0.0
25	2.3137382838077654E-4	0.0	0.0	36.01620542293979	0.0
26	2.7764859405693187E-4	0.0	0.0	36.9459580149051	0.0
27	2.7764859405693187E-4	0.0	0.0	37.883068294612926	0.0
28	2.7764859405693187E-4	0.0	0.0	38.83109941902032	0.0
29	2.7764859405693187E-4	0.0	0.0	39.76659008192947	0.0
30	2.7764859405693187E-4	0.0	0.0	40.790419272514406	0.0
31	3.701981254092425E-4	0.0	0.0	41.780838082281164	0.0
32	3.701981254092425E-4	0.0	0.0	42.69333018664927	0.0
33	3.701981254092425E-4	0.0	0.0	43.59411477530131	0.0
34	3.701981254092425E-4	0.0	0.0	44.4189161987131	0.0
35	3.701981254092425E-4	0.0	0.0	45.254869840652844	0.0
36	3.701981254092425E-4	0.0	0.0	46.07504378749702	0.0
37	4.164728910853978E-4	0.0	0.0	46.88790632136436	0.0
38	4.627476567615531E-4	0.0	0.0	47.711689699931284	0.0
39	4.627476567615531E-4	0.0	0.0	48.50692154807601	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCGT	25	3.891949E-5	45.000004	29
CGACGGT	30	2.1663927E-6	45.000004	28
CGCGCCA	30	2.1663927E-6	45.000004	39
GTATCCG	25	3.891949E-5	45.000004	1
CGACTCG	25	3.891949E-5	45.000004	2
GCGTAAC	25	3.891949E-5	45.000004	32
ACGCGCC	30	2.1663927E-6	45.000004	38
TATTTCG	20	7.0346677E-4	45.0	12
CGGCGGT	20	7.0346677E-4	45.0	30
ACCGGTA	20	7.0346677E-4	45.0	36
GTCGATG	35	1.2125929E-7	45.0	1
GGCCGTA	20	7.0346677E-4	45.0	8
ATTCGTT	35	1.2125929E-7	45.0	25
CGCGATA	20	7.0346677E-4	45.0	11
CCGTCGC	20	7.0346677E-4	45.0	19
CGTTATA	20	7.0346677E-4	45.0	4
GCCGACG	40	6.8193913E-9	45.0	1
GTTCGAC	20	7.0346677E-4	45.0	17
TCCGTCG	20	7.0346677E-4	45.0	18
CCGCGAT	20	7.0346677E-4	45.0	10
>>END_MODULE