SRR2934782.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934782.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1202621
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	10860	0.903027637135889	RNA PCR Primer, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	8838	0.7348948671277152	RNA PCR Primer, Index 16 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	7135	0.5932874945639566	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4872	0.40511516096924965	No Hit
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	4527	0.3764278189055405	RNA PCR Primer, Index 14 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	3966	0.3297797061584656	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	3699	0.30757819795263847	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	3509	0.29177937188856673	No Hit
ACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	3006	0.24995405867684	RNA PCR Primer, Index 14 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	2609	0.21694282737454276	RNA PCR Primer, Index 16 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1894	0.15748935034395709	RNA PCR Primer, Index 14 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1767	0.14692908239586702	RNA PCR Primer, Index 16 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1571	0.13063134603503515	RNA PCR Primer, Index 14 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1329	0.11050863073237537	RNA PCR Primer, Index 16 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	30	2.165585E-6	45.000004	1
ACTGCGC	20	7.033416E-4	45.000004	27
CGAGTTA	20	7.033416E-4	45.000004	14
GTCGAGT	20	7.033416E-4	45.000004	38
GTCGACG	30	2.165585E-6	45.000004	1
GATCGAC	20	7.033416E-4	45.000004	42
CCTAGCG	20	7.033416E-4	45.000004	1
ACGCAGT	20	7.033416E-4	45.000004	16
TAGCGTT	20	7.033416E-4	45.000004	27
CCGTGAT	30	2.165585E-6	45.000004	30
CGCGCCT	20	7.033416E-4	45.000004	17
GCCGGAC	20	7.033416E-4	45.000004	11
GTTAGCG	60	0.0	45.000004	1
ATTCGAT	20	7.033416E-4	45.000004	22
CGCTGTA	20	7.033416E-4	45.000004	42
TCGATTG	30	2.165585E-6	45.000004	1
TCGATAT	20	7.033416E-4	45.000004	24
AGAACCG	20	7.033416E-4	45.000004	3
AGACGCA	20	7.033416E-4	45.000004	17
GTAGCGT	20	7.033416E-4	45.000004	43