##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934782.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1202621
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0645174165427	33.0	31.0	34.0	30.0	34.0
2	32.240552094134394	34.0	31.0	34.0	30.0	34.0
3	32.25486167296264	34.0	31.0	34.0	30.0	34.0
4	35.852631045025824	37.0	35.0	37.0	35.0	37.0
5	35.72914575747472	37.0	35.0	37.0	35.0	37.0
6	35.65118353995149	37.0	35.0	37.0	33.0	37.0
7	36.01851372959561	37.0	35.0	37.0	35.0	37.0
8	35.8308286650574	37.0	35.0	37.0	35.0	37.0
9	37.55076287541961	39.0	37.0	39.0	35.0	39.0
10	37.25754497884205	39.0	37.0	39.0	34.0	39.0
11	37.119611249096764	39.0	37.0	39.0	34.0	39.0
12	37.04171555294644	39.0	37.0	39.0	33.0	39.0
13	36.984141304700316	39.0	37.0	39.0	33.0	39.0
14	38.08969741922019	40.0	37.0	41.0	33.0	41.0
15	38.13931404823298	40.0	37.0	41.0	33.0	41.0
16	38.236121770699164	40.0	37.0	41.0	34.0	41.0
17	38.215910914577414	40.0	37.0	41.0	34.0	41.0
18	38.20451663491657	40.0	37.0	41.0	34.0	41.0
19	38.22768436606379	40.0	37.0	41.0	34.0	41.0
20	38.15520600421912	40.0	37.0	41.0	34.0	41.0
21	38.0904291543221	40.0	37.0	41.0	34.0	41.0
22	38.044989236010345	40.0	37.0	41.0	34.0	41.0
23	37.86072835914224	40.0	36.0	41.0	33.0	41.0
24	37.59472685077011	39.0	36.0	41.0	33.0	41.0
25	37.52259440006453	39.0	36.0	41.0	33.0	41.0
26	37.74239432040518	39.0	36.0	41.0	33.0	41.0
27	37.830219162978196	40.0	36.0	41.0	33.0	41.0
28	37.80538091385399	40.0	36.0	41.0	33.0	41.0
29	37.84458279042192	40.0	36.0	41.0	34.0	41.0
30	37.67220928289129	40.0	36.0	41.0	33.0	41.0
31	37.493136241592325	39.0	36.0	41.0	33.0	41.0
32	37.43433633705049	39.0	36.0	41.0	33.0	41.0
33	37.31436171495425	39.0	36.0	41.0	33.0	41.0
34	37.19756265689689	39.0	36.0	41.0	32.0	41.0
35	37.21027904884415	39.0	36.0	41.0	32.0	41.0
36	37.14904196750265	39.0	35.0	41.0	32.0	41.0
37	37.05386900777552	39.0	35.0	41.0	32.0	41.0
38	36.99218290716693	39.0	35.0	41.0	31.0	41.0
39	37.038554956216466	39.0	35.0	41.0	32.0	41.0
40	36.91543969380212	39.0	35.0	41.0	31.0	41.0
41	36.87382225988071	39.0	35.0	41.0	31.0	41.0
42	36.87035400180107	39.0	35.0	41.0	31.0	41.0
43	36.85020717250073	39.0	35.0	41.0	31.0	41.0
44	36.799322479817	39.0	35.0	41.0	31.0	41.0
45	36.7537869370317	39.0	35.0	41.0	31.0	41.0
46	36.65737917432009	39.0	35.0	41.0	31.0	41.0
47	36.488853928211796	39.0	35.0	40.0	31.0	41.0
48	36.52116003296134	39.0	35.0	40.0	31.0	41.0
49	36.547462583806535	39.0	35.0	40.0	31.0	41.0
50	36.4478867407105	39.0	35.0	40.0	31.0	41.0
51	35.49638747369288	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	16.0
11	22.0
12	9.0
13	9.0
14	12.0
15	27.0
16	53.0
17	92.0
18	186.0
19	344.0
20	664.0
21	1148.0
22	1708.0
23	2821.0
24	4125.0
25	6009.0
26	8219.0
27	9887.0
28	11359.0
29	13338.0
30	16936.0
31	21806.0
32	29297.0
33	41235.0
34	71830.0
35	96852.0
36	94584.0
37	150323.0
38	276133.0
39	343364.0
40	199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.41589162337927	21.977164875717285	24.962394636381703	12.64454886452174
2	30.104413609940288	24.86992992804882	26.544023428827536	18.481633033183357
3	29.598684872457742	24.550793641554574	28.656825383890684	17.193696102097004
4	27.9970996681415	25.637420267898197	28.753613981462156	17.611866082498143
5	25.09502162360378	28.395146933239985	26.70009919999734	19.8097322431589
6	25.361855480654338	32.47872771222189	26.10015956814325	16.059257238980525
7	77.36893002866239	5.028932639626283	11.662942855646126	5.939194476065194
8	75.43714935960706	4.88291822610781	11.591764986641676	8.088167427643455
9	70.22320415159888	6.39569739760074	14.121739101512446	9.25935934928793
10	36.22421361343266	29.957235072396042	20.72606415487506	13.092487159296237
11	23.856643115328936	23.574010432214305	31.813098224627712	20.75624822782905
12	21.85160578436598	22.294721279605128	35.51983542612344	20.333837509905447
13	20.22457615491497	26.342546820652558	36.05067598187625	17.38220104255622
14	17.16135008452372	29.715762488764124	31.598982555601474	21.523904871110684
15	15.227656925997469	27.124422407391858	38.41592654710004	19.231994119510635
16	16.346130659617618	28.400052884491455	32.62091714679853	22.63289930909239
17	16.758563171606017	27.012583349201453	30.907825491156398	25.321027988036132
18	16.77211690133467	27.295798094328973	33.947270170735415	21.984814833600943
19	18.586653650651368	27.470167243046646	30.601411417229535	23.34176768907245
20	20.192230137341692	28.267509048985506	31.64180568940672	19.898455124266082
21	20.12886852965315	27.702243682756244	32.63072904930148	19.53815873828912
22	18.204820970197595	25.314874761042756	29.60467179601886	26.87563247274079
23	16.76280390912848	27.011668680324057	31.273776193829978	24.951751216717486
24	20.483760054081877	24.109673787502466	31.429186751270766	23.977379407144895
25	16.514596036490296	28.636536365155774	29.628037428250465	25.220830170103465
26	16.154299650513337	27.310017037786633	32.61642695412769	23.919256357572337
27	19.024946346355172	27.437571770324983	29.90393482235883	23.633547060961018
28	14.265841025560006	27.52596204456766	32.312590583400755	25.895606346471585
29	16.426455217396004	23.442048658721244	32.689766767751436	27.44172935613132
30	17.80993347031193	25.73853275470826	28.910604421509355	27.54092935347046
31	17.911295412270366	32.038439375331045	24.710444936517824	25.339820275880765
32	17.065393004113517	29.618973891192653	26.003953032584665	27.31168007210917
33	16.849115390467986	26.502364419048064	25.165617430595344	31.48290275988861
34	15.400446192108735	28.576334522680046	29.089546914613994	26.933672370597222
35	15.00248207872638	26.736685955093087	27.460272188827567	30.800559777352966
36	17.3151807593581	26.857006488328413	27.634558185829118	28.19325456648437
37	16.13060141141723	29.288944729885806	28.77939101346143	25.801062845235535
38	15.267652901454406	30.38222349351957	24.8405773722561	29.509546232769925
39	18.365968996051123	25.36834131451222	27.583419880411203	28.682269809025453
40	18.53584795209796	25.798651445467858	29.51678043207295	26.148720170361234
41	16.106404262024363	28.48769479328899	26.2672945175579	29.138606427128742
42	18.402722054579122	24.530837229684167	29.190659401424057	27.875781314312654
43	20.54454395857049	23.4705696973527	27.48837746887839	28.496508875198423
44	17.610535655040117	24.620391627952614	27.863724315474286	29.905348401532983
45	18.402888358011378	22.794795700391063	27.248817374717387	31.553498566880172
46	21.205101191480942	24.526014430148816	28.537585822965006	25.731298555405235
47	14.818716786086389	26.143731067393635	32.23218287390625	26.80536927261373
48	16.886201055860493	23.85905451509661	29.06626443409852	30.188479994944377
49	18.64619027939808	20.89145291825105	33.1989878773113	27.26336892503956
50	18.079095575414033	21.162278057675692	31.4947103035786	29.263916063331674
51	17.317509007409647	20.962880242403884	27.142798936655854	34.57681181353061
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	872.0
1	1062.0
2	1252.0
3	4514.0
4	7776.0
5	5132.0
6	2488.0
7	2453.5
8	2419.0
9	2524.0
10	2629.0
11	2697.0
12	2765.0
13	2934.5
14	3104.0
15	2849.0
16	2594.0
17	2572.0
18	2550.0
19	2624.5
20	2699.0
21	3170.5
22	3642.0
23	4240.0
24	4838.0
25	5435.5
26	7639.5
27	9246.0
28	10580.5
29	11915.0
30	14075.0
31	16235.0
32	19655.5
33	23076.0
34	26632.5
35	30189.0
36	32037.5
37	33886.0
38	38065.0
39	42244.0
40	48867.5
41	55491.0
42	65443.0
43	75395.0
44	89671.0
45	103947.0
46	126176.5
47	148406.0
48	163254.0
49	178102.0
50	170858.0
51	163614.0
52	128521.5
53	93429.0
54	74575.0
55	55721.0
56	47314.0
57	38907.0
58	31755.5
59	24604.0
60	21691.0
61	18778.0
62	15851.0
63	12924.0
64	10996.0
65	9068.0
66	7291.0
67	5514.0
68	4250.5
69	2987.0
70	2180.0
71	1373.0
72	1119.0
73	865.0
74	692.5
75	382.0
76	244.0
77	157.0
78	70.0
79	116.5
80	163.0
81	91.0
82	19.0
83	19.0
84	19.0
85	11.0
86	3.0
87	3.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1202621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.878980050958685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.17328911066421	14.682194013672905
2	12.490074577197626	5.715203341733814
3	6.266913222392084	4.30141747788593
4	3.645186458011268	3.335925930194582
5	2.450930405713506	2.8037393929303684
6	1.7395384340388924	2.3879319078151036
7	1.2742571879029194	2.0407593347275124
8	0.9109884226067196	1.6673988757978764
9	0.7086320218382314	1.4591500101996673
>10	4.705621075735074	22.26344878015335
>50	1.2747883007791379	20.88511263699866
>100	0.34074863589537757	11.247566107236265
>500	0.012810099093792557	1.9361834404011669
>1k	0.005124039637517022	3.0270346504221863
>5k	7.320056625024318E-4	1.337542517668361
>10k+	3.660028312512159E-4	0.909391582162299
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	10860	0.903027637135889	RNA PCR Primer, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	8838	0.7348948671277152	RNA PCR Primer, Index 16 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	7135	0.5932874945639566	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4872	0.40511516096924965	No Hit
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	4527	0.3764278189055405	RNA PCR Primer, Index 14 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	3966	0.3297797061584656	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	3699	0.30757819795263847	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	3509	0.29177937188856673	No Hit
ACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	3006	0.24995405867684	RNA PCR Primer, Index 14 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	2609	0.21694282737454276	RNA PCR Primer, Index 16 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1894	0.15748935034395709	RNA PCR Primer, Index 14 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1767	0.14692908239586702	RNA PCR Primer, Index 16 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1571	0.13063134603503515	RNA PCR Primer, Index 14 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1329	0.11050863073237537	RNA PCR Primer, Index 16 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.628959580782308	0.0
2	0.0	0.0	0.0	3.0539130781850643	0.0
3	0.0	0.0	0.0	4.25703525882219	0.0
4	0.0	0.0	0.0	5.540897755818333	0.0
5	0.0	0.0	0.0	9.071602774273856	0.0
6	0.0	0.0	0.0	10.569664092012363	0.0
7	0.0	0.0	0.0	12.305456166157086	0.0
8	0.0	0.0	0.0	14.918748300586802	0.0
9	0.0	0.0	0.0	15.805644504794111	0.0
10	0.0	0.0	0.0	18.03394419355724	0.0
11	0.0	0.0	0.0	20.870581837503252	0.0
12	0.0	0.0	0.0	23.442464417301878	0.0
13	0.0	0.0	0.0	24.31497537461927	0.0
14	0.0	0.0	0.0	24.644505625629353	0.0
15	0.0	0.0	0.0	25.32385514638444	0.0
16	0.0	0.0	0.0	26.66658905839828	0.0
17	0.0	0.0	0.0	28.4955110546049	0.0
18	0.0	0.0	0.0	30.459804044665777	0.0
19	0.0	0.0	0.0	31.621849277536313	0.0
20	0.0	0.0	0.0	32.64062410352056	0.0
21	0.0	0.0	0.0	33.83667838828692	0.0
22	0.0	0.0	0.0	35.11887785096053	0.0
23	0.0	0.0	0.0	36.39633766581492	0.0
24	0.0	0.0	0.0	37.34875742233006	0.0
25	0.0	0.0	0.0	38.129468885043586	0.0
26	0.0	0.0	0.0	38.88041203338375	0.0
27	0.0	0.0	0.0	39.55618603034539	0.0
28	0.0	0.0	0.0	40.266052230918966	0.0
29	8.315171612669328E-5	0.0	0.0	41.041608287232634	0.0
30	8.315171612669328E-5	0.0	0.0	41.91636434088545	0.0
31	8.315171612669328E-5	0.0	0.0	42.667307489225614	0.0
32	8.315171612669328E-5	0.0	0.0	43.38083236530877	0.0
33	8.315171612669328E-5	0.0	0.0	44.05660636227041	0.0
34	8.315171612669328E-5	0.0	0.0	44.780192596004895	0.0
35	8.315171612669328E-5	0.0	0.0	45.508102718977966	0.0
36	8.315171612669328E-5	0.0	0.0	46.19668208022311	0.0
37	8.315171612669328E-5	0.0	0.0	46.853497485907866	0.0
38	8.315171612669328E-5	0.0	0.0	47.49784013417361	0.0
39	8.315171612669328E-5	0.0	0.0	48.15989409797434	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	30	2.165585E-6	45.000004	1
ACTGCGC	20	7.033416E-4	45.000004	27
CGAGTTA	20	7.033416E-4	45.000004	14
GTCGAGT	20	7.033416E-4	45.000004	38
GTCGACG	30	2.165585E-6	45.000004	1
GATCGAC	20	7.033416E-4	45.000004	42
CCTAGCG	20	7.033416E-4	45.000004	1
ACGCAGT	20	7.033416E-4	45.000004	16
TAGCGTT	20	7.033416E-4	45.000004	27
CCGTGAT	30	2.165585E-6	45.000004	30
CGCGCCT	20	7.033416E-4	45.000004	17
GCCGGAC	20	7.033416E-4	45.000004	11
GTTAGCG	60	0.0	45.000004	1
ATTCGAT	20	7.033416E-4	45.000004	22
CGCTGTA	20	7.033416E-4	45.000004	42
TCGATTG	30	2.165585E-6	45.000004	1
TCGATAT	20	7.033416E-4	45.000004	24
AGAACCG	20	7.033416E-4	45.000004	3
AGACGCA	20	7.033416E-4	45.000004	17
GTAGCGT	20	7.033416E-4	45.000004	43
>>END_MODULE