##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934781.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1306355
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.132010824010315	33.0	31.0	34.0	30.0	34.0
2	32.30227541518194	34.0	31.0	34.0	30.0	34.0
3	32.305383299332874	34.0	31.0	34.0	30.0	34.0
4	35.88008466305101	37.0	35.0	37.0	35.0	37.0
5	35.76846492722116	37.0	35.0	37.0	35.0	37.0
6	35.70178244045455	37.0	35.0	37.0	33.0	37.0
7	36.02622258115137	37.0	35.0	37.0	35.0	37.0
8	35.81496606971306	37.0	35.0	37.0	35.0	37.0
9	37.45653669944234	39.0	37.0	39.0	35.0	39.0
10	37.29051368119692	39.0	37.0	39.0	34.0	39.0
11	37.199960960075934	39.0	37.0	39.0	34.0	39.0
12	37.08253728886864	39.0	37.0	39.0	34.0	39.0
13	36.97714403818258	39.0	37.0	39.0	33.0	39.0
14	38.07797803812899	40.0	37.0	41.0	33.0	41.0
15	38.134149599458034	40.0	37.0	41.0	33.0	41.0
16	38.24665730218815	40.0	37.0	41.0	34.0	41.0
17	38.24016672344041	40.0	37.0	41.0	34.0	41.0
18	38.21246598359558	40.0	37.0	41.0	34.0	41.0
19	38.18888127652897	40.0	37.0	41.0	34.0	41.0
20	38.138926248990515	40.0	36.0	41.0	34.0	41.0
21	38.06047973177276	40.0	36.0	41.0	34.0	41.0
22	38.01039992957504	40.0	36.0	41.0	34.0	41.0
23	37.81842531318057	40.0	36.0	41.0	33.0	41.0
24	37.56954120434338	39.0	35.0	41.0	33.0	41.0
25	37.48283812593055	39.0	35.0	41.0	33.0	41.0
26	37.652862353648125	39.0	36.0	41.0	33.0	41.0
27	37.73204527100214	39.0	36.0	41.0	33.0	41.0
28	37.68539868565589	39.0	36.0	41.0	33.0	41.0
29	37.685216499343596	40.0	36.0	41.0	33.0	41.0
30	37.57017579448159	40.0	36.0	41.0	33.0	41.0
31	37.464627149587976	39.0	36.0	41.0	33.0	41.0
32	37.37855559935852	39.0	35.0	41.0	33.0	41.0
33	37.24575172904762	39.0	35.0	41.0	33.0	41.0
34	37.18382522361839	39.0	35.0	41.0	32.0	41.0
35	37.11812026593078	39.0	35.0	41.0	32.0	41.0
36	37.076425627030936	39.0	35.0	41.0	32.0	41.0
37	37.002296466121386	39.0	35.0	41.0	32.0	41.0
38	36.924176047092864	39.0	35.0	41.0	31.0	41.0
39	36.9602106624922	39.0	35.0	41.0	32.0	41.0
40	36.872706117403006	39.0	35.0	41.0	31.0	41.0
41	36.81022616363852	39.0	35.0	41.0	31.0	41.0
42	36.724098732733445	39.0	35.0	41.0	31.0	41.0
43	36.667004757512316	39.0	35.0	41.0	31.0	41.0
44	36.60578326718235	39.0	35.0	41.0	31.0	41.0
45	36.58679838175687	39.0	35.0	41.0	31.0	41.0
46	36.52913488293764	39.0	35.0	41.0	31.0	41.0
47	36.410807935055935	39.0	35.0	40.0	31.0	41.0
48	36.39553337339391	39.0	35.0	40.0	31.0	41.0
49	36.378980445590976	39.0	35.0	40.0	31.0	41.0
50	36.28606083338756	39.0	35.0	40.0	31.0	41.0
51	35.40775286962579	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	16.0
10	23.0
11	28.0
12	13.0
13	23.0
14	22.0
15	38.0
16	78.0
17	126.0
18	274.0
19	457.0
20	911.0
21	1462.0
22	2220.0
23	3265.0
24	4808.0
25	7132.0
26	9771.0
27	11729.0
28	13232.0
29	15141.0
30	18445.0
31	23454.0
32	31218.0
33	44286.0
34	82046.0
35	110680.0
36	101223.0
37	152545.0
38	282833.0
39	388580.0
40	269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.62298915685246	21.41347489771157	24.293702707150814	12.669833238285152
2	32.00033681503114	25.35436385974716	24.476960703637218	18.168338621584486
3	31.84410056990634	22.979894439107287	28.099865656731897	17.076139334254474
4	28.96555683562278	25.431372023684222	26.939002032372517	18.66406910832048
5	24.77366412651997	29.58613853049133	25.756934370825697	19.883262972163003
6	26.521504491504988	32.75487903364706	25.183812975799075	15.53980349904888
7	76.89012557842241	6.07155022945524	11.099815899965936	5.93850829215642
8	74.9633905025816	5.8255221589843496	11.270979174879722	7.940108163554317
9	68.94603687359103	7.883308901485431	14.464598061017107	8.706056163906442
10	37.53252370144409	26.13860704019964	21.09043866330362	15.238430595052646
11	28.767678004830234	25.250640139931335	25.775918490762468	20.205763364475963
12	27.782800234239545	21.385840755384255	31.287284084341543	19.544074926034654
13	23.855383873449405	24.766545081543683	31.201166604789666	20.176904440217246
14	17.877070168522337	29.036287992161398	29.20469550772952	23.881946331586743
15	16.59280976457395	27.460376390797297	36.87940873652261	19.067405108106144
16	18.652586777713562	26.428727260201097	33.37094434514355	21.547741616941796
17	18.677694807307354	26.093290108737673	29.573584515694435	25.65543056826054
18	19.133007490307	27.290897191039186	31.56194143245902	22.014153886194794
19	21.84390919772956	28.25082002977751	28.181926046136002	21.723344726356924
20	24.286277466691672	27.271377229007427	28.43300634207394	20.00933896222696
21	22.053193810258314	27.635214011505294	29.803001481220647	20.50859069701574
22	19.401694026508874	26.4072170275308	27.57504659912505	26.61604234683528
23	19.922379445097235	27.08452143559752	29.76311951957929	23.229979599725954
24	21.801654221096104	24.401406968243702	29.641559912887384	24.15537889777281
25	19.639837563296346	27.55016821614339	27.580634666687082	25.22935955387318
26	18.665064243639744	28.12826528776634	29.45194836013182	23.754722108462094
27	20.70180004669481	27.398831098744214	28.302490517508637	23.59687833705233
28	16.52475781851028	27.345399986986692	30.08539026528011	26.044451929222912
29	18.74291444515465	26.000512877433774	30.428635401556235	24.827937275855337
30	20.304894152049023	24.602960144830462	28.960963903380016	26.1311817997405
31	18.864397502975837	27.729522220223444	25.009051903961787	28.39702837283893
32	20.12615253893467	27.635520206988147	26.564907701199136	25.673419552878045
33	18.999889004137465	26.185454949075865	28.511698581166684	26.302957465619986
34	17.636400519001345	24.105775229550925	29.93060844869886	28.327215802748867
35	17.463323522319737	23.59595975060378	29.49627015627452	29.444446570801965
36	18.13572880266084	24.691833383728007	30.45917840097064	26.713259412640518
37	18.28117165701513	25.80041412939056	28.776481124962206	27.141933088632108
38	17.494096168346278	27.515950870934773	27.204167320521606	27.785785640197343
39	20.129061396021754	23.992942194120282	31.16090189879474	24.717094511063227
40	20.855892923439647	21.449835611300145	32.07466576849325	25.619605696766957
41	16.646929816167887	23.630023998070968	29.086810246831835	30.636235938929314
42	19.412946710503654	22.824270584948195	30.341675884426518	27.421106820121636
43	21.14050162474978	22.966115642378988	29.59624298142542	26.29713975144582
44	18.974551327931536	24.733322871654337	28.028598658098296	28.26352714231583
45	19.57178561723268	22.51577863597567	27.921812983453965	29.990622763337683
46	20.345158858043945	23.418749114903683	29.38940793275947	26.8466840942929
47	17.171672324904026	22.912761079492174	32.711093079599344	27.204473516004455
48	17.326913434709553	20.776741391122627	32.22562014153886	29.670725032628958
49	18.937195479023696	20.762732947782187	33.954706033199244	26.34536553999487
50	18.318221310440116	20.902281539091593	32.1078879783826	28.671609172085688
51	17.158429370270714	20.290502964355017	29.154785644024788	33.396282021349485
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	955.0
1	1408.5
2	1862.0
3	5922.5
4	9983.0
5	6780.5
6	3578.0
7	3401.0
8	3224.0
9	3226.5
10	3229.0
11	3262.5
12	3296.0
13	3176.5
14	3057.0
15	3117.0
16	3177.0
17	3149.0
18	3121.0
19	3087.5
20	3054.0
21	3292.5
22	3531.0
23	4088.5
24	4646.0
25	5677.0
26	9144.5
27	11581.0
28	12803.0
29	14025.0
30	16185.5
31	18346.0
32	21370.5
33	24395.0
34	25773.0
35	27151.0
36	29931.5
37	32712.0
38	35342.5
39	37973.0
40	45418.5
41	52864.0
42	63665.0
43	74466.0
44	83851.0
45	93236.0
46	111120.5
47	129005.0
48	150562.5
49	172120.0
50	166888.5
51	161657.0
52	130705.0
53	99753.0
54	83796.5
55	67840.0
56	59168.5
57	50497.0
58	48418.5
59	46340.0
60	45390.5
61	44441.0
62	40492.5
63	36544.0
64	30611.5
65	24679.0
66	18108.5
67	11538.0
68	9970.5
69	8403.0
70	6647.0
71	4891.0
72	4417.5
73	3944.0
74	3122.0
75	1657.5
76	1015.0
77	845.5
78	676.0
79	441.5
80	207.0
81	193.0
82	179.0
83	143.5
84	108.0
85	67.0
86	26.0
87	16.5
88	7.0
89	7.5
90	8.0
91	4.5
92	1.0
93	2.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1306355.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.843322708731016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.2750945914302	15.139433733037139
2	12.316229329489968	5.626872022565544
3	5.7834555029931485	3.9634002127937626
4	3.4534583357777877	3.1555385290131666
5	2.150197856904642	2.4558831766447287
6	1.4754997063492057	2.0223189569263655
7	1.1017337884697844	1.761708232838966
8	0.8029293742105654	1.4673259845929114
9	0.6148920116253971	1.2641558987321757
>10	4.1562201017737	19.741005855186327
>50	1.3675819028252651	22.75935974251908
>100	0.4891938557271087	15.581532751545653
>500	0.008446026514542604	1.3598852339164322
>1k	0.003716251666398746	1.4750290264936592
>5k	0.001351364242326817	2.226550643194086
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	9548	0.7308886175656694	No Hit
CCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	7608	0.5823838083828669	No Hit
CTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGCT	5962	0.456384367189623	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5733	0.4388546757963953	No Hit
TCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	3474	0.2659307768562144	No Hit
ACTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	2710	0.20744743963164686	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTG	2298	0.1759093048979795	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTG	1729	0.13235299746240495	No Hit
AGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTG	1480	0.11329232865492152	No Hit
ACCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTG	1415	0.1083166520585905	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.65488707127848E-5	0.0	0.0	0.4798083216277352	0.0
2	7.65488707127848E-5	0.0	0.0	2.3676565711464344	0.0
3	1.530977414255696E-4	0.0	0.0	3.3122696357421986	0.0
4	1.530977414255696E-4	0.0	0.0	4.396890584871647	0.0
5	1.530977414255696E-4	0.0	0.0	7.007360173919034	0.0
6	1.530977414255696E-4	0.0	0.0	8.41134301166222	0.0
7	1.530977414255696E-4	0.0	0.0	9.860566231996662	0.0
8	1.530977414255696E-4	0.0	0.0	12.08561225700518	0.0
9	1.530977414255696E-4	0.0	0.0	12.836939423051161	0.0
10	2.2964661213835443E-4	0.0	0.0	14.598941329118043	0.0
11	2.2964661213835443E-4	0.0	0.0	17.10606994270317	0.0
12	2.2964661213835443E-4	0.0	0.0	19.081796295800146	0.0
13	2.2964661213835443E-4	0.0	0.0	19.865962927381915	0.0
14	2.2964661213835443E-4	0.0	0.0	20.15968094430687	0.0
15	2.2964661213835443E-4	0.0	0.0	20.76357498536003	0.0
16	2.2964661213835443E-4	0.0	0.0	21.991648518205235	0.0
17	2.2964661213835443E-4	0.0	0.0	23.38575655162647	0.0
18	2.2964661213835443E-4	0.0	0.0	25.077486594379018	0.0
19	2.2964661213835443E-4	0.0	0.0	25.985815494256922	0.0
20	3.061954828511392E-4	0.0	0.0	26.88909216866778	0.0
21	3.8274435356392404E-4	0.0	0.0	27.919439968461866	0.0
22	3.8274435356392404E-4	0.0	0.0	29.049377849053283	0.0
23	3.8274435356392404E-4	0.0	0.0	30.0997049041034	0.0
24	3.8274435356392404E-4	0.0	0.0	30.906606550286867	0.0
25	3.8274435356392404E-4	0.0	0.0	31.63680622801612	0.0
26	3.8274435356392404E-4	0.0	0.0	32.25493835902186	0.0
27	3.8274435356392404E-4	0.0	0.0	32.88906920400657	0.0
28	3.8274435356392404E-4	0.0	0.0	33.54478683053228	0.0
29	3.8274435356392404E-4	0.0	0.0	34.20999651702638	0.0
30	3.8274435356392404E-4	0.0	0.0	34.983522855579075	0.0
31	3.8274435356392404E-4	0.0	0.0	35.68784901500741	0.0
32	3.8274435356392404E-4	0.0	0.0	36.32664934110559	0.0
33	3.8274435356392404E-4	0.0	0.0	36.95833062222749	0.0
34	3.8274435356392404E-4	0.0	0.0	37.53704008481615	0.0
35	3.8274435356392404E-4	0.0	0.0	38.18556211749486	0.0
36	3.8274435356392404E-4	0.0	0.0	38.831558037440054	0.0
37	4.5929322427670885E-4	0.0	0.0	39.46614817564904	0.0
38	4.5929322427670885E-4	0.0	0.0	40.09293032904532	0.0
39	4.5929322427670885E-4	0.0	0.0	40.718487700510195	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	30	2.1657306E-6	45.000004	28
AACGACT	30	2.1657306E-6	45.000004	21
ACCACGT	30	2.1657306E-6	45.000004	38
ATAACGG	30	2.1657306E-6	45.000004	2
TCGACAG	30	2.1657306E-6	45.000004	1
TCCGGAC	20	7.033641E-4	45.0	31
AACCGTA	20	7.033641E-4	45.0	22
AAGTACG	35	1.2121018E-7	45.0	1
AATCCCG	20	7.033641E-4	45.0	1
TCGTTAC	25	3.8910992E-5	45.0	26
CGAGTTA	20	7.033641E-4	45.0	20
GACGTGA	20	7.033641E-4	45.0	10
ACCGAAT	25	3.8910992E-5	45.0	10
CGCGATA	20	7.033641E-4	45.0	11
ATATCGT	20	7.033641E-4	45.0	15
CTAACGG	50	2.1827873E-11	45.0	2
TCTACGG	50	2.1827873E-11	45.0	2
GTTCGGA	20	7.033641E-4	45.0	27
CGTAGTC	25	3.8910992E-5	45.0	22
CGTAGAC	20	7.033641E-4	45.0	10
>>END_MODULE