##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934780.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	653040
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11839091020458	33.0	31.0	34.0	30.0	34.0
2	32.30025878966067	34.0	31.0	34.0	30.0	34.0
3	32.29785618032586	34.0	31.0	34.0	30.0	34.0
4	35.87761546000245	37.0	35.0	37.0	35.0	37.0
5	35.77464780105353	37.0	35.0	37.0	35.0	37.0
6	35.708148046061496	37.0	35.0	37.0	33.0	37.0
7	36.03663787823104	37.0	35.0	37.0	35.0	37.0
8	35.86554881783658	37.0	35.0	37.0	35.0	37.0
9	37.58018651231165	39.0	38.0	39.0	35.0	39.0
10	37.33729939973049	39.0	37.0	39.0	34.0	39.0
11	37.223595798113436	39.0	37.0	39.0	34.0	39.0
12	37.04294530197232	39.0	37.0	39.0	33.0	39.0
13	36.879094695577606	39.0	35.0	39.0	33.0	39.0
14	37.94803687369839	40.0	37.0	41.0	33.0	41.0
15	38.06191810608845	40.0	37.0	41.0	33.0	41.0
16	38.182867818204095	40.0	37.0	41.0	34.0	41.0
17	38.17019784392993	40.0	37.0	41.0	34.0	41.0
18	38.15683572216097	40.0	37.0	41.0	34.0	41.0
19	38.11821940463065	40.0	37.0	41.0	34.0	41.0
20	38.04780258483401	40.0	36.0	41.0	34.0	41.0
21	37.94674751929438	40.0	36.0	41.0	34.0	41.0
22	37.883472681612155	40.0	36.0	41.0	34.0	41.0
23	37.73512801666054	39.0	35.0	41.0	33.0	41.0
24	37.45935011637878	39.0	35.0	41.0	33.0	41.0
25	37.39399730491241	39.0	35.0	41.0	33.0	41.0
26	37.58534852382702	39.0	35.0	41.0	33.0	41.0
27	37.68459971824084	39.0	35.0	41.0	33.0	41.0
28	37.69485942668137	39.0	36.0	41.0	33.0	41.0
29	37.68640665196619	39.0	36.0	41.0	33.0	41.0
30	37.484143390910205	39.0	36.0	41.0	33.0	41.0
31	37.361437277961535	39.0	35.0	41.0	33.0	41.0
32	37.20855996569888	39.0	35.0	41.0	33.0	41.0
33	37.058050042876395	39.0	35.0	41.0	32.0	41.0
34	37.05220354036506	39.0	35.0	41.0	32.0	41.0
35	36.907230797500915	39.0	35.0	41.0	31.0	41.0
36	36.83069796643391	39.0	35.0	41.0	31.0	41.0
37	36.7870421413696	39.0	35.0	41.0	31.0	41.0
38	36.66051849810119	39.0	35.0	41.0	31.0	41.0
39	36.708961166237906	39.0	35.0	41.0	31.0	41.0
40	36.60770243782923	39.0	35.0	41.0	31.0	41.0
41	36.51955316672792	39.0	35.0	41.0	31.0	41.0
42	36.47688043611417	39.0	35.0	41.0	31.0	41.0
43	36.46805861815509	39.0	35.0	41.0	31.0	41.0
44	36.37052554208012	39.0	35.0	40.0	31.0	41.0
45	36.310892135244394	39.0	35.0	40.0	31.0	41.0
46	36.22836885948794	39.0	35.0	40.0	30.0	41.0
47	36.085175486953325	38.0	35.0	40.0	30.0	41.0
48	36.07639807668749	38.0	35.0	40.0	30.0	41.0
49	36.08707889256401	38.0	35.0	40.0	30.0	41.0
50	35.97371830209482	38.0	35.0	40.0	30.0	41.0
51	34.939363591816736	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	10.0
11	18.0
12	8.0
13	6.0
14	13.0
15	21.0
16	40.0
17	60.0
18	131.0
19	265.0
20	452.0
21	736.0
22	1155.0
23	1709.0
24	2561.0
25	3733.0
26	4939.0
27	6162.0
28	6767.0
29	7952.0
30	9469.0
31	12229.0
32	15766.0
33	23448.0
34	43914.0
35	61654.0
36	51743.0
37	78490.0
38	142893.0
39	176589.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.60954918534852	21.09380742374127	24.17187308587529	13.124770305034913
2	32.230338110988605	24.18274531422271	25.46275878966067	18.124157785128016
3	30.826748744334193	24.398505451427173	28.064283964228835	16.7104618400098
4	28.359977949283348	24.867389440156806	27.029431581526396	19.743201029033443
5	24.369104495896117	28.59916697292662	27.295571481073132	19.73615705010413
6	25.4250888153865	31.9328065662134	27.10170893054024	15.540395687859856
7	75.8968822736739	6.063640818326595	12.048725958593655	5.9907509494058555
8	74.82236922699987	4.766323655518804	11.955010412838417	8.4562967046429
9	69.55791375719711	6.240505941443097	15.056811221364693	9.144769079995099
10	38.09046919024868	24.97289599411981	21.61843072399853	15.318204091632978
11	28.773428886438808	24.777808403773122	26.347696925150068	20.101065784638
12	26.206817346563767	21.951794683327208	32.25009187798604	19.591296092122995
13	22.226509861570502	27.35896729143697	32.66262403528115	17.75189881171138
14	17.115184368491974	29.408918289844422	31.491945363224303	21.9839519784393
15	15.222497856180325	26.604495896116624	38.05249295602107	20.12051329168198
16	17.81253828249418	25.725376699742743	34.02685899791743	22.435226019845643
17	17.53200416513537	25.955071664829106	29.572614234962636	26.940309935072893
18	18.76470047776553	25.20473477888031	33.754440769325	22.276123974029154
19	19.994640450814654	27.82218547102781	29.68424598799461	22.49892809016293
20	22.9560210706848	26.44309690064927	30.03001347543795	20.57086855322798
21	20.500428763934828	27.268008085262775	31.67968271468823	20.55188043611417
22	18.91201151537425	25.697813303932378	28.892410878353548	26.497764302339828
23	19.097298787210583	26.40450814651476	29.335569030993508	25.162624035281144
24	21.82347176283229	23.432714688227367	30.734564498346195	24.009249050594146
25	18.457828004410143	26.80279921597452	29.018589979174326	25.720782800441018
26	17.929529584711503	27.1917493568541	31.462391277716527	23.416329780717874
27	22.085170893054023	26.741087835354648	30.056504961411246	21.117236310180083
28	17.4134815631508	24.487780227857407	32.12222834742129	25.976509861570502
29	20.968393972804115	22.596165625382824	33.19597574421169	23.23946465760137
30	23.755359549185346	23.516629915472254	30.76610927355139	21.96190126179101
31	24.33342521131937	24.79909347053779	26.31278329045694	24.5546980276859
32	24.638000735023887	24.80475927967659	29.257932132794316	21.299307852505205
33	23.057699375229696	22.980828126914123	31.759769692515004	22.20170280534117
34	21.851035158642656	21.89008330270734	32.33523214504471	23.92364939360529
35	22.277961533749846	20.514363591816736	33.38095675609457	23.826718118338846
36	23.555218669606763	21.151690554943038	34.08657968883988	21.206511086610313
37	23.31235452652211	22.567377189758666	32.897678549552865	21.222589734166363
38	20.51528237167708	24.69328065662134	30.843133651843686	23.948303319857896
39	22.863224304789906	21.673557515619258	33.72228347421291	21.740934705377928
40	24.33342521131937	20.684337865980645	34.39620850177631	20.58602842092368
41	20.37991547225285	21.971395320347913	31.826687492343503	25.822001715055737
42	21.896208501776307	20.09892196496386	34.02747151782433	23.977398015435504
43	24.686083547715302	20.935930417738575	30.43136714443219	23.94661889011393
44	21.00575768712483	22.735207644248437	30.07243047899057	26.186604189636164
45	22.50352198946466	21.362397402915594	28.625658458899917	27.508422148719834
46	25.331220139654537	21.234993262281023	30.913879701090284	22.519906896974152
47	19.586549062844544	21.839703540365065	35.46061496998652	23.11313242680387
48	20.25986157050104	19.490383437461716	33.7601065784638	26.48964841357344
49	21.889164522846993	17.7835967168933	36.31400833027074	24.013230429988973
50	20.854465270121278	18.44511821634203	34.6961901261791	26.004226387357587
51	19.585936542937645	19.239097145657233	30.531667279186575	30.643299032218547
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	656.0
1	787.5
2	919.0
3	3017.5
4	5116.0
5	3374.5
6	1633.0
7	1670.5
8	1708.0
9	1774.5
10	1841.0
11	1910.5
12	1980.0
13	1916.5
14	1853.0
15	1831.0
16	1809.0
17	1803.5
18	1798.0
19	1643.5
20	1489.0
21	1583.0
22	1677.0
23	1844.5
24	2012.0
25	2337.0
26	3634.5
27	4607.0
28	5285.0
29	5963.0
30	7833.5
31	9704.0
32	10261.5
33	10819.0
34	12049.5
35	13280.0
36	14959.0
37	16638.0
38	18263.5
39	19889.0
40	23399.0
41	26909.0
42	32773.5
43	38638.0
44	43313.5
45	47989.0
46	58614.0
47	69239.0
48	80113.5
49	90988.0
50	88894.5
51	86801.0
52	68815.5
53	50830.0
54	42422.5
55	34015.0
56	29987.0
57	25959.0
58	22564.5
59	19170.0
60	17631.5
61	16093.0
62	15232.5
63	14372.0
64	11886.5
65	9401.0
66	7378.0
67	5355.0
68	4447.5
69	3540.0
70	2967.0
71	2394.0
72	1861.0
73	1328.0
74	1143.5
75	695.5
76	432.0
77	420.0
78	408.0
79	274.5
80	141.0
81	77.0
82	13.0
83	9.0
84	5.0
85	4.5
86	4.0
87	3.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	653040.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.146157207982917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.60897693598588	16.498136482872265
2	12.999178872558101	6.537587910080724
3	6.187675196985824	4.6678875976602665
4	3.4578330157549373	3.4780485045250935
5	2.14952994551496	2.702620896659307
6	1.4538308659811994	2.1934955705868715
7	1.0535938207371789	1.854568509473153
8	0.7321821169455938	1.4729253294070108
9	0.5963443525810547	1.3496191956086396
>10	4.193282397037325	23.611970748108806
>50	1.4005209100958202	24.244733386450974
>100	0.15349018777018453	6.179686630762999
>500	0.009246396853625573	1.6064984132547655
>1k	0.003082132284541858	1.8338945124678818
>5k	0.0012328529138167432	1.7683263120812778
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	5815	0.8904508146514762	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	5593	0.856455959818694	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	3594	0.5503491363469313	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3118	0.4774592674261914	No Hit
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	2060	0.3154477520519417	TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	1563	0.23934215361999267	No Hit
ACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	1496	0.22908244517946832	TruSeq Adapter, Index 21 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	976	0.14945485728286168	No Hit
ACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	928	0.142104618400098	No Hit
TGGCAAGGGAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCT	886	0.1356731593776798	No Hit
GGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	828	0.12679162072767367	No Hit
AGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	784	0.12005390175180693	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCC	714	0.1093348033811099	No Hit
AAAAGAAGGGAAACTGATTATTCAAGGCCATGGCACTGTTACCAGAAAAAT	703	0.1076503736371432	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5312997672424353E-4	0.0	0.0	0.5846502511331618	0.0
2	1.5312997672424353E-4	0.0	0.0	3.0273796398382946	0.0
3	1.5312997672424353E-4	0.0	0.0	4.213218179590837	0.0
4	1.5312997672424353E-4	0.0	0.0	5.3661337743476665	0.0
5	1.5312997672424353E-4	0.0	0.0	8.462881293642043	0.0
6	1.5312997672424353E-4	0.0	0.0	9.890358936665441	0.0
7	1.5312997672424353E-4	0.0	0.0	11.394248438074237	0.0
8	1.5312997672424353E-4	0.0	0.0	13.714014455469803	0.0
9	1.5312997672424353E-4	0.0	0.0	14.471089060394462	0.0
10	1.5312997672424353E-4	0.0	0.0	16.298082812691412	0.0
11	1.5312997672424353E-4	0.0	0.0	18.83483400710523	0.0
12	1.5312997672424353E-4	0.0	0.0	20.877587896606638	0.0
13	1.5312997672424353E-4	0.0	0.0	21.677845154967535	0.0
14	1.5312997672424353E-4	0.0	0.0	21.993599166972928	0.0
15	1.5312997672424353E-4	0.0	0.0	22.60137204459145	0.0
16	1.5312997672424353E-4	0.0	0.0	23.843256155825063	0.0
17	1.5312997672424353E-4	0.0	0.0	25.40334435869166	0.0
18	1.5312997672424353E-4	0.0	0.0	27.15392625260321	0.0
19	1.5312997672424353E-4	0.0	0.0	28.11634815631508	0.0
20	1.5312997672424353E-4	0.0	0.0	28.990414063457063	0.0
21	1.5312997672424353E-4	0.0	0.0	30.078555678059537	0.0
22	1.5312997672424353E-4	0.0	0.0	31.177263261055984	0.0
23	1.5312997672424353E-4	0.0	0.0	32.21425946343256	0.0
24	1.5312997672424353E-4	0.0	0.0	33.10241332843317	0.0
25	1.5312997672424353E-4	0.0	0.0	33.806198701457795	0.0
26	1.5312997672424353E-4	0.0	0.0	34.483339458532406	0.0
27	3.0625995344848707E-4	0.0	0.0	35.134907509494056	0.0
28	3.0625995344848707E-4	0.0	0.0	35.75661521499449	0.0
29	3.0625995344848707E-4	0.0	0.0	36.461931887786356	0.0
30	3.0625995344848707E-4	0.0	0.0	37.2119625137817	0.0
31	3.0625995344848707E-4	0.0	0.0	37.95127404140634	0.0
32	3.0625995344848707E-4	0.0	0.0	38.615551880436115	0.0
33	3.0625995344848707E-4	0.0	0.0	39.265894891583976	0.0
34	3.0625995344848707E-4	0.0	0.0	39.91608477275511	0.0
35	3.0625995344848707E-4	0.0	0.0	40.60501653803749	0.0
36	3.0625995344848707E-4	0.0	0.0	41.22825554330516	0.0
37	3.0625995344848707E-4	0.0	0.0	41.83755972069092	0.0
38	7.656498836212177E-4	0.0	0.0	42.44701702805341	0.0
39	7.656498836212177E-4	0.0	0.0	43.051880436114175	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACA	25	3.888948E-5	45.000004	13
GCCCAAT	25	3.888948E-5	45.000004	9
CTGTACG	55	1.8189894E-12	45.000004	1
ATCTACG	25	3.888948E-5	45.000004	1
GTCGACA	25	3.888948E-5	45.000004	9
CGCGGTC	25	3.888948E-5	45.000004	19
TTGTCGA	25	3.888948E-5	45.000004	7
CGCGAAA	25	3.888948E-5	45.000004	29
AGGCGCC	25	3.888948E-5	45.000004	24
TGCGAAC	25	3.888948E-5	45.000004	1
GTCACAC	25	3.888948E-5	45.000004	27
GGACGTT	25	3.888948E-5	45.000004	8
GAACGAT	25	3.888948E-5	45.000004	43
CCGACGG	50	2.1827873E-11	45.000004	2
TTCGGTC	25	3.888948E-5	45.000004	14
ACGTAAG	50	2.1827873E-11	45.000004	1
CCGCGAA	25	3.888948E-5	45.000004	28
TTCGCGC	25	3.888948E-5	45.000004	21
TGTCGAC	25	3.888948E-5	45.000004	8
CGCCCAT	25	3.888948E-5	45.000004	33
>>END_MODULE