##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934779.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1248706
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.163930500854484	33.0	31.0	34.0	30.0	34.0
2	32.334948338520036	34.0	31.0	34.0	30.0	34.0
3	32.332302399443904	34.0	31.0	34.0	30.0	34.0
4	35.90273931573965	37.0	35.0	37.0	35.0	37.0
5	35.79304175682667	37.0	35.0	37.0	35.0	37.0
6	35.727036628317634	37.0	35.0	37.0	33.0	37.0
7	36.06143960227627	37.0	35.0	37.0	35.0	37.0
8	35.90221237024568	37.0	35.0	37.0	35.0	37.0
9	37.5871910601855	39.0	38.0	39.0	35.0	39.0
10	37.242185910854914	39.0	37.0	39.0	34.0	39.0
11	37.17676618835819	39.0	37.0	39.0	34.0	39.0
12	37.11371691975533	39.0	37.0	39.0	34.0	39.0
13	37.056101276040955	39.0	37.0	39.0	33.0	39.0
14	38.18752692787574	40.0	37.0	41.0	33.0	41.0
15	38.25239087503383	40.0	37.0	41.0	33.0	41.0
16	38.34373263202067	40.0	37.0	41.0	34.0	41.0
17	38.342283932326744	40.0	37.0	41.0	34.0	41.0
18	38.3180236180494	40.0	37.0	41.0	34.0	41.0
19	38.2790680912881	40.0	37.0	41.0	34.0	41.0
20	38.23978662711639	40.0	37.0	41.0	34.0	41.0
21	38.16791462522003	40.0	37.0	41.0	34.0	41.0
22	38.11023091103911	40.0	37.0	41.0	34.0	41.0
23	37.945468348834716	40.0	36.0	41.0	34.0	41.0
24	37.70102329931945	39.0	36.0	41.0	33.0	41.0
25	37.63649409869097	39.0	36.0	41.0	33.0	41.0
26	37.82826461953414	40.0	36.0	41.0	33.0	41.0
27	37.92063624263838	40.0	36.0	41.0	34.0	41.0
28	37.89725764111008	40.0	36.0	41.0	34.0	41.0
29	37.90090541728798	40.0	36.0	41.0	34.0	41.0
30	37.80042059540036	40.0	36.0	41.0	33.0	41.0
31	37.71012792442737	40.0	36.0	41.0	33.0	41.0
32	37.58419515882842	40.0	36.0	41.0	33.0	41.0
33	37.502923826745445	40.0	36.0	41.0	33.0	41.0
34	37.45614420047633	40.0	36.0	41.0	33.0	41.0
35	37.31796355587304	40.0	36.0	41.0	33.0	41.0
36	37.296390823780776	40.0	36.0	41.0	33.0	41.0
37	37.218233114920565	40.0	36.0	41.0	32.0	41.0
38	37.124815609118556	40.0	35.0	41.0	32.0	41.0
39	37.194792849557864	40.0	36.0	41.0	32.0	41.0
40	37.12788118260023	39.0	36.0	41.0	32.0	41.0
41	37.05372841966003	39.0	35.0	41.0	32.0	41.0
42	36.960621635517086	39.0	35.0	41.0	31.0	41.0
43	36.94793249972371	39.0	35.0	41.0	31.0	41.0
44	36.928719810748085	39.0	35.0	41.0	31.0	41.0
45	36.88226612188938	39.0	35.0	41.0	31.0	41.0
46	36.81450637700147	39.0	35.0	41.0	31.0	41.0
47	36.69098891172141	39.0	35.0	41.0	31.0	41.0
48	36.656003094403324	39.0	35.0	41.0	31.0	41.0
49	36.661403084473044	39.0	35.0	41.0	31.0	41.0
50	36.56469176891918	39.0	35.0	41.0	31.0	41.0
51	35.58666411469153	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	10.0
10	21.0
11	15.0
12	18.0
13	17.0
14	20.0
15	39.0
16	55.0
17	83.0
18	223.0
19	436.0
20	744.0
21	1232.0
22	1817.0
23	2775.0
24	4224.0
25	6714.0
26	9044.0
27	10606.0
28	11771.0
29	13190.0
30	16502.0
31	21003.0
32	27983.0
33	39840.0
34	69850.0
35	97428.0
36	91865.0
37	144076.0
38	277476.0
39	399377.0
40	250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.750655478551394	22.091429047349816	23.432737569932392	11.725177904166394
2	33.533353727778994	24.323820018483133	24.66168978126156	17.481136472476308
3	31.592064104761246	23.53204036818915	27.53562487887461	17.34027064817499
4	28.130801005200585	26.621238305894263	26.46627789087263	18.781682798032524
5	24.115604473751226	30.0932325142988	26.42719743478449	19.36396557716548
6	25.538997970699267	33.68495066092419	26.371379652215975	14.40467171616057
7	78.6792087168637	5.883050133498197	10.221221007987468	5.216520141650636
8	78.81350774321577	4.603085113709712	10.120716966203414	6.462690176871097
9	73.01310316439579	6.738896105248153	12.84497712031495	7.4030236100411155
10	37.08839390537084	23.649922399668135	23.20970668836379	16.05197700659723
11	27.990655927015645	27.036307986027136	24.741051936965146	20.231984149992073
12	24.890406548859378	23.28978959018376	31.313776020936874	20.50602784001999
13	22.990679951886193	25.518016250422438	32.319296936188344	19.17200686150303
14	20.064931216795625	28.417337627912413	30.576692992585926	20.941038162706032
15	19.205241265758314	26.832657166698965	34.77840260237397	19.183698965168745
16	20.527249809002278	26.346393786848143	31.612725493430798	21.513630910718774
17	20.800412587110177	27.1597157377317	28.472114332757272	23.567757342400853
18	21.079021002541833	26.22138437710718	32.21422816900055	20.485366451350437
19	20.90988591389807	28.6330809654154	29.083547288152694	21.373485832533838
20	22.37308061305063	27.347510142499516	30.17499715705699	20.10441208739287
21	21.465741335430437	27.467153997818546	31.303044912092997	19.76405975465802
22	19.867286615103954	26.20528771384137	28.861797733013216	25.065627938041462
23	19.07758912025729	27.60521692055616	29.76088847174595	23.5563054874406
24	21.461737190339438	25.241329824634462	30.731012744392995	22.5659202406331
25	19.163598156811933	27.66327702437563	29.271982356135073	23.901142462677363
26	18.73971935747886	27.354877769466952	31.63330679919853	22.272096073855653
27	21.571370682930972	27.34398649481944	29.6579819429073	21.426660879342297
28	17.1918770311026	26.192874864059274	32.12837929824955	24.48686880658858
29	18.96283032194928	25.854284355164463	31.716432851287653	23.466452471598597
30	21.847416445504386	25.55004941115042	28.984484738601402	23.61804940474379
31	21.05859986257774	27.790448672465732	26.71717762227458	24.433773842681944
32	20.70327202720256	29.45264938264091	27.601372941268803	22.24270564888773
33	20.945442722306133	26.413743507278735	27.69571059961272	24.945103170802415
34	19.9440861179493	25.370823876877342	29.633636740754028	25.051453264419326
35	19.180255400390482	27.394999303278755	29.083307039447238	24.341438256883528
36	22.3111765299438	25.31556667462157	29.38369800417392	22.989558791260713
37	21.404638081341805	26.59545161150823	29.69177692747532	22.30813337967464
38	18.77223301561777	29.78579425421196	26.87101687667073	24.570955853499544
39	20.86391832825341	25.71117620961219	29.67071512429667	23.75419033783773
40	22.67603423063555	24.549173304204512	29.636119310710445	23.138673154449485
41	20.482083052375817	25.806474862777947	28.127677772029603	25.583764312816626
42	20.797930017153757	24.737207957677786	31.007939418886433	23.456922606282024
43	21.517074475497033	24.340156930454405	29.956691166695766	24.186077427352796
44	20.45717726990981	24.874550134299025	27.886948569158793	26.78132402663237
45	20.999338515230967	24.293869013202467	27.190467572030563	27.516324899536
46	23.249187558961037	25.165170985003677	28.346624425605388	23.239017030429903
47	19.785521972345773	24.970569533581163	31.354778466668698	23.889130027404367
48	19.76381950595256	24.14987995573017	30.218722421450682	25.86757811686658
49	20.825798866987107	21.824913150092975	33.11764338443156	24.231644598488355
50	20.941038162706032	22.115293752092168	30.694655106966735	26.24901297823507
51	19.850148874114485	22.050106270010716	27.971436030578857	30.128308825295946
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	693.0
1	844.0
2	995.0
3	4093.5
4	7192.0
5	5030.5
6	2869.0
7	2834.5
8	2800.0
9	2908.5
10	3017.0
11	3062.5
12	3108.0
13	3079.0
14	3050.0
15	2952.0
16	2854.0
17	2897.5
18	2941.0
19	3066.0
20	3191.0
21	3199.0
22	3207.0
23	3955.5
24	4704.0
25	5618.5
26	7584.5
27	8636.0
28	9891.0
29	11146.0
30	13902.5
31	16659.0
32	19343.5
33	22028.0
34	25594.0
35	29160.0
36	32202.5
37	35245.0
38	39501.5
39	43758.0
40	49329.5
41	54901.0
42	65333.0
43	75765.0
44	86702.5
45	97640.0
46	114985.5
47	132331.0
48	152798.0
49	173265.0
50	167157.0
51	161049.0
52	133598.5
53	106148.0
54	89824.5
55	73501.0
56	62513.5
57	51526.0
58	43778.5
59	36031.0
60	31493.0
61	26955.0
62	22316.5
63	17678.0
64	14724.0
65	11770.0
66	9583.0
67	7396.0
68	5601.0
69	3806.0
70	2950.5
71	2095.0
72	1714.0
73	1333.0
74	1081.0
75	725.0
76	621.0
77	390.0
78	159.0
79	109.5
80	60.0
81	49.0
82	38.0
83	24.5
84	11.0
85	8.0
86	5.0
87	4.5
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1248706.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.60893757623537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.1065088477024	16.92917458463662
2	12.888515360320529	6.6012237062559835
3	5.8782712521774885	4.5160884465957665
4	3.425628659634988	3.5090684201582096
5	2.117156243956054	2.7109061045303764
6	1.4385112395544706	2.2103246721878045
7	1.0161007500997243	1.8214882476328842
8	0.7503642520686895	1.5372825032532489
9	0.5853612328389183	1.349143134418803
>10	4.273483991714086	23.930336358205352
>50	1.2964065524363566	23.17514078830891
>100	0.21422652789955332	8.02960217162421
>500	0.005994556744643774	0.9992968368919185
>1k	0.0028395268790417875	1.571581724966422
>5k	6.310059731203972E-4	1.1093423003336063
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	7883	0.6312935150467764	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	5847	0.4682447269413297	No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT	4056	0.3248162497817741	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4039	0.3234548404508347	No Hit
TCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	2643	0.21165910951016492	No Hit
ACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	2054	0.16449028033820612	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	1732	0.1387035859521777	No Hit
ACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	1444	0.11563971022802806	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6016580363992805E-4	0.0	0.0	0.34275481978944605	0.0
2	1.6016580363992805E-4	0.0	0.0	1.9074145555479032	0.0
3	1.6016580363992805E-4	0.0	0.0	2.720256009020538	0.0
4	1.6016580363992805E-4	0.0	0.0	3.589315659570788	0.0
5	1.6016580363992805E-4	0.0	0.0	5.819944806864066	0.0
6	1.6016580363992805E-4	0.0	0.0	6.963929059362251	0.0
7	1.6016580363992805E-4	0.0	0.0	8.145632358617641	0.0
8	1.6016580363992805E-4	0.0	0.0	10.046480116216307	0.0
9	1.6016580363992805E-4	0.0	0.0	10.669364926571987	0.0
10	1.6016580363992805E-4	0.0	0.0	12.24699809242528	0.0
11	1.6016580363992805E-4	0.0	0.0	14.486676607624213	0.0
12	1.6016580363992805E-4	0.0	0.0	16.152080633872185	0.0
13	1.6016580363992805E-4	0.0	0.0	16.810041755225008	0.0
14	1.6016580363992805E-4	0.0	0.0	17.061902481448797	0.0
15	1.6016580363992805E-4	0.0	0.0	17.5902093847551	0.0
16	1.6016580363992805E-4	0.0	0.0	18.80875081884767	0.0
17	1.6016580363992805E-4	0.0	0.0	20.260573745941798	0.0
18	1.6016580363992805E-4	0.0	0.0	21.846775782289825	0.0
19	1.6016580363992805E-4	0.0	0.0	22.77061213768493	0.0
20	1.6016580363992805E-4	0.0	0.0	23.61748882443105	0.0
21	1.6016580363992805E-4	0.0	0.0	24.73216273486313	0.0
22	2.402487054598921E-4	0.0	0.0	25.93789090466451	0.0
23	2.402487054598921E-4	0.0	0.0	27.095409167570267	0.0
24	2.402487054598921E-4	0.0	0.0	28.03173845564929	0.0
25	2.402487054598921E-4	0.0	0.0	28.882138790075487	0.0
26	3.203316072798561E-4	0.0	0.0	29.62947242985939	0.0
27	3.203316072798561E-4	0.0	0.0	30.374163333883235	0.0
28	3.203316072798561E-4	0.0	0.0	31.110605699019626	0.0
29	3.203316072798561E-4	0.0	0.0	31.880522717116758	0.0
30	3.203316072798561E-4	0.0	0.0	32.7234753416737	0.0
31	3.203316072798561E-4	0.0	0.0	33.54120185215735	0.0
32	3.203316072798561E-4	0.0	0.0	34.25297868353319	0.0
33	4.004145090998201E-4	0.0	0.0	34.987819390633184	0.0
34	4.004145090998201E-4	0.0	0.0	35.68654270901237	0.0
35	4.004145090998201E-4	0.0	0.0	36.43539792393085	0.0
36	4.004145090998201E-4	0.0	0.0	37.14405152213571	0.0
37	4.004145090998201E-4	0.0	0.0	37.87296609450103	0.0
38	4.004145090998201E-4	0.0	0.0	38.5672047703783	0.0
39	4.004145090998201E-4	0.0	0.0	39.255036814109964	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGT	35	1.2120472E-7	45.000004	20
GTATCCG	45	3.8562575E-10	45.000004	17
ATGCGCG	35	1.2120472E-7	45.000004	1
TTACACG	45	3.8562575E-10	45.000004	34
TCCGCTC	25	3.891001E-5	45.0	44
TCACCGA	20	7.033522E-4	45.0	40
ACGCATA	25	3.891001E-5	45.0	39
GCGGTAA	20	7.033522E-4	45.0	30
CCGGCCG	20	7.033522E-4	45.0	44
CGTTGTA	20	7.033522E-4	45.0	18
CCGGACA	25	3.891001E-5	45.0	19
CGCGCAA	20	7.033522E-4	45.0	18
ATCGTCG	25	3.891001E-5	45.0	28
AGACGAT	20	7.033522E-4	45.0	16
CACTCGA	20	7.033522E-4	45.0	33
CGCTCGT	20	7.033522E-4	45.0	21
CGGATCG	20	7.033522E-4	45.0	10
CCGAGTA	25	3.891001E-5	45.0	12
GACGGTA	20	7.033522E-4	45.0	35
GTCCGGT	20	7.033522E-4	45.0	26
>>END_MODULE