##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934778.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1939398 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06197077649869 33.0 31.0 34.0 30.0 34.0 2 32.235141523297436 34.0 31.0 34.0 30.0 34.0 3 32.248171855390176 34.0 31.0 34.0 30.0 34.0 4 35.84383659259213 37.0 35.0 37.0 35.0 37.0 5 35.721454286330086 37.0 35.0 37.0 35.0 37.0 6 35.62797785704636 37.0 35.0 37.0 33.0 37.0 7 35.96891561195794 37.0 35.0 37.0 35.0 37.0 8 35.686852311902975 37.0 35.0 37.0 35.0 37.0 9 37.46238884437336 39.0 37.0 39.0 35.0 39.0 10 37.20227307649075 39.0 37.0 39.0 34.0 39.0 11 37.11839859585294 39.0 37.0 39.0 34.0 39.0 12 37.07471339044384 39.0 37.0 39.0 33.0 39.0 13 37.02763383276666 39.0 37.0 39.0 33.0 39.0 14 38.15889466731429 40.0 37.0 41.0 33.0 41.0 15 38.16087466316868 40.0 37.0 41.0 33.0 41.0 16 38.29909951438539 40.0 37.0 41.0 34.0 41.0 17 38.23270777839309 40.0 37.0 41.0 34.0 41.0 18 38.21531939292502 40.0 37.0 41.0 34.0 41.0 19 38.21359514653516 40.0 37.0 41.0 34.0 41.0 20 38.107441071920256 40.0 37.0 41.0 34.0 41.0 21 38.12849966845382 40.0 37.0 41.0 34.0 41.0 22 38.083146419662185 40.0 37.0 41.0 34.0 41.0 23 37.85098004638553 40.0 36.0 41.0 33.0 41.0 24 37.619379828173486 39.0 36.0 41.0 33.0 41.0 25 37.5616098397544 39.0 36.0 41.0 33.0 41.0 26 37.71614645369336 39.0 36.0 41.0 33.0 41.0 27 37.794639883097744 40.0 36.0 41.0 33.0 41.0 28 37.74447483188082 40.0 36.0 41.0 33.0 41.0 29 37.74988578930163 40.0 36.0 41.0 33.0 41.0 30 37.688643073778564 40.0 36.0 41.0 33.0 41.0 31 37.566118970938405 40.0 36.0 41.0 33.0 41.0 32 37.459885490239756 40.0 36.0 41.0 33.0 41.0 33 37.32772798569453 39.0 36.0 41.0 32.0 41.0 34 37.21588090737435 39.0 36.0 41.0 32.0 41.0 35 37.23447739968795 40.0 36.0 41.0 32.0 41.0 36 37.00068474856631 39.0 35.0 41.0 31.0 41.0 37 37.00445756879196 39.0 35.0 41.0 31.0 41.0 38 36.90306837482559 39.0 35.0 41.0 31.0 41.0 39 36.980455790920686 39.0 35.0 41.0 31.0 41.0 40 36.99326956096686 39.0 35.0 41.0 31.0 41.0 41 36.81042158442981 39.0 35.0 41.0 31.0 41.0 42 36.733016637121416 39.0 35.0 41.0 31.0 41.0 43 36.62352492886968 39.0 35.0 41.0 31.0 41.0 44 36.728964864354815 39.0 35.0 41.0 31.0 41.0 45 36.75833789660503 39.0 35.0 41.0 31.0 41.0 46 36.70988110743643 39.0 35.0 41.0 31.0 41.0 47 36.50727184414958 39.0 35.0 40.0 31.0 41.0 48 36.470347499584925 39.0 35.0 40.0 31.0 41.0 49 36.50683150132154 39.0 35.0 40.0 31.0 41.0 50 36.38196904400231 39.0 35.0 40.0 30.0 41.0 51 35.46663036674267 38.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 7.0 9 18.0 10 25.0 11 23.0 12 25.0 13 35.0 14 17.0 15 62.0 16 70.0 17 171.0 18 348.0 19 727.0 20 1504.0 21 2662.0 22 4045.0 23 5849.0 24 7755.0 25 10534.0 26 13307.0 27 15493.0 28 17658.0 29 21177.0 30 27242.0 31 36047.0 32 48495.0 33 67121.0 34 109627.0 35 146461.0 36 155187.0 37 242464.0 38 444650.0 39 560248.0 40 343.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.794157774732156 21.82440118016003 23.704262869199617 9.677178175908194 2 30.403609779942027 29.536897532120793 24.25866170842705 15.800830979510136 3 28.925264437727584 28.85410833671067 26.534161631599083 15.686465593962662 4 26.62939736969926 24.074429281663694 31.89422697146228 17.401946377174774 5 28.231389328028595 27.742732538653748 24.94222433971779 19.08365379359987 6 24.474192507159437 36.30229586706803 24.883597899966897 14.339913725805637 7 75.26469553954371 5.15804388784561 14.258238896812308 5.319021675798366 8 74.33090061967683 8.383890258729771 10.474229632081707 6.810979489511694 9 69.22019100772508 6.317527397677011 12.079263771541477 12.383017823056433 10 37.92692371550347 25.232108107773648 21.18776032562682 15.653207851096063 11 27.553601684646473 24.833273005334643 27.106452620864825 20.50667268915406 12 24.05401057441536 21.266547660665836 32.60583954402346 22.07360222089535 13 23.356113598137153 24.041893412285667 34.955279937382635 17.646713052194546 14 20.134134406656088 29.194420124182862 28.56932924546689 22.102116223694157 15 17.307690324523385 26.413815008574826 36.12450873930983 20.153985927591965 16 19.12480058244878 26.97966069883541 29.473733601870272 24.421805116845537 17 19.97289880674312 26.907112413233385 29.611456751012426 23.508532029011064 18 19.940156687796932 26.611247407700738 31.30636413980008 22.14223176470224 19 20.146148444001696 28.727213289897175 28.09418180280685 23.032456463294277 20 21.433042624567005 28.230358080187766 31.049841239394905 19.28675805585032 21 21.333063146399038 29.5147772659351 30.059946436987143 19.092213150678717 22 19.043074191063415 24.771759071629443 29.342043252596937 26.8431234847102 23 18.93494785495293 28.383601509334337 29.478477341938063 23.20297329377467 24 21.368434947339328 25.20142848450911 28.527048083993073 24.903088484158488 25 18.449333246708516 31.352151543932706 26.016165841152773 24.18234936820601 26 18.24726023229889 28.672299342373254 29.489924192971223 23.59051623235664 27 22.695805605657014 28.26542050677581 27.03519339506383 22.003580492503342 28 16.602677738143488 28.452849801845726 31.144973852710994 23.79949860729979 29 20.236795129210197 27.37282393814988 28.868184869737927 23.522196062901994 30 24.85596045783279 28.308011042601876 26.764181462495063 20.071847037070267 31 23.125217206576473 30.246860108136648 23.706737864017597 22.92118482126928 32 24.301767868173528 29.60310364350175 26.334821423967643 19.760307064357082 33 25.53637778320902 25.277843949514228 25.665490012880287 23.52028825439647 34 23.9451623648163 27.728862255194652 26.8413703633808 21.484605016608246 35 25.591446417909065 24.906852538777496 26.632697362789898 22.869003680523544 36 24.906233790073003 28.799297513970828 27.193025877102066 19.101442818854096 37 24.497704957930246 25.74371016160685 31.1043426877825 18.65424219268041 38 23.126609391161587 27.367100512633304 27.019982489411664 22.48630760679345 39 25.66677907268132 23.154710894824063 31.018336617857706 20.160173414636915 40 28.052055328509155 22.515646607864916 28.9684221598661 20.463875903759828 41 24.587939144002416 25.636460386161065 26.52529290016799 23.25030756966853 42 23.992806015062406 23.871737518549573 30.85189321634858 21.283563250039446 43 23.611141189173136 24.39530204733634 29.78341732846997 22.210139435020558 44 22.66404317215961 24.522764280462287 28.442021699517067 24.371170847861038 45 23.226073245409143 23.051276736389333 27.165800934104293 26.556849084097227 46 27.957232089545315 24.516731480593464 26.456818043537222 21.069218386324003 47 21.44526291148078 23.711172229733144 33.45749557336865 21.386069285417435 48 23.03781895206657 23.623567725655075 29.249179384530667 24.089433937747692 49 22.940056656756376 20.625317753240953 33.852566621188636 22.582058968814035 50 23.33198239866185 20.10948758326037 31.386595221816254 25.171934796261517 51 22.63099167886117 20.050139270020903 26.951868569525182 30.367000481592743 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 585.0 1 977.0 2 1369.0 3 4851.5 4 8334.0 5 5735.5 6 3137.0 7 3150.5 8 3164.0 9 3325.0 10 3486.0 11 3533.5 12 3581.0 13 3603.0 14 3625.0 15 3731.0 16 3837.0 17 3824.5 18 3812.0 19 3844.0 20 3876.0 21 4081.0 22 4286.0 23 4968.0 24 5650.0 25 6395.5 26 9754.0 27 12367.0 28 14813.0 29 17259.0 30 19535.5 31 21812.0 32 26279.0 33 30746.0 34 35843.0 35 40940.0 36 46386.0 37 51832.0 38 57198.5 39 62565.0 40 71725.0 41 80885.0 42 95606.5 43 110328.0 44 130114.0 45 149900.0 46 221191.5 47 292483.0 48 283993.5 49 275504.0 50 260909.5 51 246315.0 52 201022.0 53 155729.0 54 127405.5 55 99082.0 56 84780.5 57 70479.0 58 62393.0 59 54307.0 60 47371.0 61 40435.0 62 34000.5 63 27566.0 64 23198.0 65 18830.0 66 14354.0 67 9878.0 68 8131.0 69 6384.0 70 4674.0 71 2964.0 72 2593.0 73 2222.0 74 1928.5 75 1081.5 76 528.0 77 407.0 78 286.0 79 195.5 80 105.0 81 95.0 82 85.0 83 54.0 84 23.0 85 20.5 86 18.0 87 12.5 88 7.0 89 8.5 90 10.0 91 7.0 92 4.0 93 2.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1939398.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.075923435906596 #Duplication Level Percentage of deduplicated Percentage of total 1 65.78207875756632 16.495463703795103 2 13.358045490360137 6.699306519392563 3 6.1154844711233896 4.600542611140976 4 3.3368587203826032 3.3469925515500556 5 2.0910794724177872 2.6217874374372196 6 1.42472725274733 2.1435810904164954 7 0.9861356723247036 1.7309783831641377 8 0.7441228276726528 1.4927653642903806 9 0.571478978340987 1.289732679549784 >10 4.344493260504924 23.942065057952785 >50 1.0324015951133139 18.143341051367106 >100 0.19700706396519255 7.524453086726727 >500 0.01002790907884505 1.7363249493565391 >1k 0.004178295449518771 2.143898208649137 >5k 0.0010445738623796926 1.622120617061184 >10k+ 8.356590899037541E-4 4.466646688149808 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC 25617 1.3208737969204878 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCG 24578 1.2673004715896377 No Hit GAATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTC 20844 1.0747664997076412 No Hit GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC 14223 0.7333719020025802 No Hit CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC 9376 0.4834489877786818 No Hit CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT 6345 0.3271633775016784 Illumina Single End Adapter 1 (95% over 21bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATGGGTT 5168 0.2664744420691369 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5040 0.25987445588785796 No Hit GAATGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCT 5035 0.25961664392765177 No Hit TCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC 4357 0.22465734212368996 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTA 3497 0.1803136849682221 No Hit GAATGATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTC 3483 0.1795918114796447 No Hit ACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC 3370 0.17376526117898441 No Hit GAACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT 3283 0.16927933307139637 No Hit CGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG 2988 0.15406842741923008 No Hit AGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG 2164 0.11158101637724696 No Hit ACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG 2124 0.1095185206955973 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.3488711445510411 0.0 2 0.0 0.0 0.0 2.075644091620183 0.0 3 0.0 0.0 0.0 2.926320435516588 0.0 4 5.1562392041241666E-5 0.0 0.0 4.023207201409922 0.0 5 5.1562392041241666E-5 0.0 0.0 7.5111452110397146 0.0 6 5.1562392041241666E-5 0.0 0.0 8.791026906287415 0.0 7 5.1562392041241666E-5 0.0 0.0 10.320573703798807 0.0 8 5.1562392041241666E-5 0.0 0.0 12.412099012167694 0.0 9 5.1562392041241666E-5 0.0 0.0 13.063074211688368 0.0 10 5.1562392041241666E-5 0.0 0.0 16.07168822490278 0.0 11 5.1562392041241666E-5 0.0 0.0 18.566792375778462 0.0 12 5.1562392041241666E-5 0.0 0.0 21.808829337763573 0.0 13 5.1562392041241666E-5 0.0 0.0 22.57355117412723 0.0 14 5.1562392041241666E-5 0.0 0.0 22.88210052810202 0.0 15 5.1562392041241666E-5 0.0 0.0 23.605624013224723 0.0 16 5.1562392041241666E-5 0.0 0.0 24.829096451579304 0.0 17 5.1562392041241666E-5 0.0 0.0 26.376329149560842 0.0 18 5.1562392041241666E-5 0.0 0.0 27.975124239583625 0.0 19 5.1562392041241666E-5 0.0 0.0 29.24665282732064 0.0 20 5.1562392041241666E-5 0.0 0.0 30.19540084087949 0.0 21 1.0312478408248333E-4 0.0 0.0 31.35576091137559 0.0 22 1.0312478408248333E-4 0.0 0.0 32.63471448356655 0.0 23 1.5468717612372498E-4 0.0 0.0 33.855763489495196 0.0 24 1.5468717612372498E-4 0.0 0.0 34.80404744152567 0.0 25 2.0624956816496666E-4 0.0 0.0 35.612133249596006 0.0 26 2.0624956816496666E-4 0.0 0.0 36.38108320210704 0.0 27 2.0624956816496666E-4 0.0 0.0 37.150084717010124 0.0 28 2.0624956816496666E-4 0.0 0.0 37.874381638013446 0.0 29 2.0624956816496666E-4 0.0 0.0 38.63492692062176 0.0 30 2.0624956816496666E-4 0.0 0.0 39.47498141175767 0.0 31 3.0937435224744997E-4 0.0 0.0 40.276312546470606 0.0 32 3.0937435224744997E-4 0.0 0.0 41.037476577783416 0.0 33 4.124991363299333E-4 0.0 0.0 41.7569781963269 0.0 34 4.124991363299333E-4 0.0 0.0 42.512160990162926 0.0 35 4.124991363299333E-4 0.0 0.0 43.236200099206044 0.0 36 4.124991363299333E-4 0.0 0.0 43.96369388851592 0.0 37 4.124991363299333E-4 0.0 0.0 44.652309634226704 0.0 38 4.124991363299333E-4 0.0 0.0 45.36345814525951 0.0 39 4.124991363299333E-4 0.0 0.0 46.067697295758784 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTACCG 30 2.1662763E-6 45.000004 10 ACGCGAT 20 7.034488E-4 45.0 1 TCCGATC 20 7.034488E-4 45.0 39 CGCTATC 25 3.891801E-5 45.0 26 TACGACG 25 3.891801E-5 45.0 1 TCTCGCA 20 7.034488E-4 45.0 44 ACTACGT 25 3.891801E-5 45.0 17 CTCGCGT 20 7.034488E-4 45.0 43 CGATCGC 25 3.891801E-5 45.0 34 TGCGTCG 20 7.034488E-4 45.0 1 TACCGAC 20 7.034488E-4 45.0 43 TGCGTAG 70 0.0 41.785717 1 CGTTTTT 3250 0.0 41.607693 1 TACGGCT 2905 0.0 41.359726 7 CGACGGT 60 3.6379788E-12 41.250004 28 GTCATCG 55 6.184564E-11 40.909092 43 TTACGCG 110 0.0 40.909092 1 TACGGGT 155 0.0 40.64516 4 TACTCGC 95 0.0 40.26316 45 ACGGCTG 3015 0.0 39.850746 8 >>END_MODULE