##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934777.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	893747
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06003153017576	33.0	31.0	34.0	30.0	34.0
2	32.24462068124424	34.0	31.0	34.0	30.0	34.0
3	32.25429344098497	34.0	31.0	34.0	30.0	34.0
4	35.83505622955937	37.0	35.0	37.0	35.0	37.0
5	35.730253640012215	37.0	35.0	37.0	35.0	37.0
6	35.64079879428966	37.0	35.0	37.0	33.0	37.0
7	35.989810315447215	37.0	35.0	37.0	35.0	37.0
8	35.80076576480816	37.0	35.0	37.0	35.0	37.0
9	37.49208221118504	39.0	37.0	39.0	35.0	39.0
10	37.24671635261433	39.0	37.0	39.0	34.0	39.0
11	37.083747413977335	39.0	37.0	39.0	33.0	39.0
12	36.92322435767617	39.0	35.0	39.0	33.0	39.0
13	36.8066264837812	39.0	35.0	39.0	33.0	39.0
14	37.875258881987854	40.0	37.0	41.0	33.0	41.0
15	37.978432375157624	40.0	37.0	41.0	33.0	41.0
16	38.08146600771807	40.0	37.0	41.0	34.0	41.0
17	38.07572165277198	40.0	37.0	41.0	34.0	41.0
18	38.067657849480895	40.0	37.0	41.0	34.0	41.0
19	38.041657202765435	40.0	37.0	41.0	34.0	41.0
20	37.98709478185661	40.0	36.0	41.0	34.0	41.0
21	37.890098652079395	40.0	36.0	41.0	34.0	41.0
22	37.8128765746906	39.0	36.0	41.0	33.0	41.0
23	37.64237754084769	39.0	35.0	41.0	33.0	41.0
24	37.345812629301136	39.0	35.0	41.0	32.0	41.0
25	37.2568937294335	39.0	35.0	41.0	32.0	41.0
26	37.44897940916165	39.0	35.0	41.0	33.0	41.0
27	37.540034260254856	39.0	35.0	41.0	33.0	41.0
28	37.49031437308321	39.0	35.0	41.0	33.0	41.0
29	37.54603036429773	39.0	36.0	41.0	33.0	41.0
30	37.399674628278476	39.0	35.0	41.0	33.0	41.0
31	37.297823657030456	39.0	35.0	41.0	33.0	41.0
32	37.25388169135113	39.0	35.0	41.0	33.0	41.0
33	37.0734850019077	39.0	35.0	41.0	32.0	41.0
34	37.00719890528304	39.0	35.0	41.0	32.0	41.0
35	36.981311265939915	39.0	35.0	41.0	32.0	41.0
36	36.89769588037778	39.0	35.0	41.0	31.0	41.0
37	36.86603703285158	39.0	35.0	41.0	31.0	41.0
38	36.7780356185811	39.0	35.0	41.0	31.0	41.0
39	36.7821374505313	39.0	35.0	41.0	31.0	41.0
40	36.681202845995564	39.0	35.0	41.0	31.0	41.0
41	36.64027515616836	39.0	35.0	41.0	31.0	41.0
42	36.5538748661534	39.0	35.0	41.0	31.0	41.0
43	36.50068755475543	39.0	35.0	40.0	31.0	41.0
44	36.4453284878159	39.0	35.0	40.0	31.0	41.0
45	36.43104368462216	39.0	35.0	40.0	31.0	41.0
46	36.36410415923074	39.0	35.0	40.0	31.0	41.0
47	36.23454624183354	38.0	35.0	40.0	30.0	41.0
48	36.226904258140166	38.0	35.0	40.0	30.0	41.0
49	36.219355141891384	38.0	35.0	40.0	30.0	41.0
50	36.115042623919294	38.0	35.0	40.0	30.0	41.0
51	35.16007214569671	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	9.0
10	19.0
11	24.0
12	6.0
13	25.0
14	14.0
15	28.0
16	50.0
17	77.0
18	199.0
19	353.0
20	616.0
21	960.0
22	1522.0
23	2211.0
24	3378.0
25	4947.0
26	6836.0
27	8428.0
28	9646.0
29	11437.0
30	13929.0
31	17529.0
32	22921.0
33	32395.0
34	60221.0
35	82341.0
36	70157.0
37	106718.0
38	193353.0
39	243239.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.89434705794817	22.59666326152703	23.30267961738613	13.206310063138673
2	32.14332467689402	24.35185796427848	24.507606738819824	18.997210620007678
3	30.890621171315818	23.72774398123854	28.527088762255985	16.854546085189657
4	29.16731468748986	25.15644807758795	26.3877808820617	19.288456352860486
5	24.664921952185573	28.65016609845963	25.743526971279344	20.94138497807545
6	25.4738757165059	32.511661577605295	26.244899283578015	15.769563422310789
7	76.84691529034502	5.950565428471368	11.332737340656807	5.869781940526794
8	75.4705750061259	5.0414714678762556	11.372681530679264	8.115271995318587
9	69.84101764817112	6.631630651627363	14.907910180397806	8.619441519803702
10	40.99448725422295	23.879912324181227	21.16583328391592	13.9597671376799
11	28.44955004044769	24.94385995141802	26.353766781874512	20.252823226259782
12	26.1066051130801	21.587429104657136	31.962736658137036	20.34322912412573
13	22.083430769557825	25.82856222174732	33.06987324153256	19.018133767162297
14	17.72738817584842	29.363678982978403	30.940859102184397	21.968073738988775
15	16.856783854938815	25.39286845158641	37.348712778896044	20.401634914578732
16	18.78462249383774	25.744086413716634	33.0477193210159	22.423571771429724
17	18.7940211267842	25.748226287752573	28.567928060178104	26.88982452528512
18	19.640233757427996	25.935751392731948	32.3794093854301	22.04460546440995
19	20.5079289776637	26.7372086283926	29.432714179739904	23.322148214203796
20	22.986147086367843	26.92753094555842	29.31265783269762	20.77366413537612
21	21.508995274949175	27.463812465943942	30.790369086553575	20.236823172553304
22	19.57746431596414	25.58721875430071	28.741355215737784	26.09396171399736
23	18.94260903812824	26.11924851216284	29.93688370422502	25.001258745483902
24	21.93529041216362	23.877115111994783	30.500242238575346	23.68735223726625
25	18.301823670457075	26.41866210460007	28.42638912354391	26.853125101398938
26	17.677150244979845	26.724229563847484	29.685022718957377	25.913597472215294
27	19.884038771598675	26.078968656678008	29.00317427638918	25.033818295334136
28	16.204362084572033	24.82917425177371	32.969621156770316	25.996842506883937
29	18.139249698180805	22.437445943874497	29.735484426800873	29.687819931143828
30	19.840178484515192	25.149510991365563	27.92389792637066	27.086412597748584
31	19.734779529329888	25.58207188387765	28.333633567441346	26.349515019351117
32	20.91330096772353	25.60098103825803	25.432365087659036	28.053352906359407
33	20.54865638709836	27.132622543068674	26.31192048756527	26.006800582267687
34	17.85617182491242	24.54866981371686	31.305839348271935	26.289319013098783
35	19.176008422965335	24.39325670463789	27.062916015382427	29.367818857014345
36	20.564208886855003	26.962328265157815	27.259056533896057	25.214406314091125
37	19.400009174855974	25.016139914315794	31.93577153265969	23.648079378168543
38	19.575002769240065	27.79914226285515	26.70946028350305	25.916394684401737
39	20.245214809112646	26.528424710796234	28.908964169949662	24.31739631014146
40	21.858646798254988	23.757953872852163	30.697837307425928	23.685562021466925
41	20.137466195690727	24.78318808342853	27.55735124145871	27.52199447942203
42	21.22781950596757	23.61932403689187	28.098779632267295	27.054076824873256
43	22.012717245484463	22.71906926680593	28.582585452035087	26.685628035674526
44	18.877266161452848	23.837394698947243	28.84104785806274	28.444291281537172
45	20.310557685788037	21.910563056435432	27.903086667703498	29.87579259007303
46	22.833195524012947	23.693506104076434	28.48099070542335	24.992307666487275
47	17.961347003122807	23.481029866393957	32.418458467552895	26.139164662930337
48	19.156987380097497	21.743401656173393	30.847991657594378	28.251619306134735
49	20.617244029910033	20.906811435450972	33.032838152184006	25.44310638245499
50	19.717772479236295	20.530306675155273	32.24156276888202	27.51035807672641
51	18.43183809288311	21.076993824874375	28.016094040035938	32.47507404220657
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	658.0
1	869.0
2	1080.0
3	3883.5
4	6687.0
5	4411.5
6	2136.0
7	2134.0
8	2132.0
9	2159.0
10	2186.0
11	2252.0
12	2318.0
13	2231.5
14	2145.0
15	2142.5
16	2140.0
17	2088.5
18	2037.0
19	1963.5
20	1890.0
21	1973.5
22	2057.0
23	2348.5
24	2640.0
25	3398.5
26	4489.5
27	4822.0
28	5980.0
29	7138.0
30	8228.5
31	9319.0
32	10304.0
33	11289.0
34	14436.5
35	17584.0
36	19121.5
37	20659.0
38	23829.0
39	26999.0
40	30701.5
41	34404.0
42	41660.5
43	48917.0
44	57296.0
45	65675.0
46	79037.0
47	92399.0
48	107898.5
49	123398.0
50	121726.0
51	120054.0
52	98867.5
53	77681.0
54	64566.0
55	51451.0
56	45040.0
57	38629.0
58	33996.0
59	29363.0
60	27010.0
61	24657.0
62	21999.0
63	19341.0
64	15842.5
65	12344.0
66	10008.5
67	7673.0
68	6700.0
69	5727.0
70	5061.5
71	4396.0
72	3379.5
73	2363.0
74	1858.0
75	1213.0
76	1073.0
77	746.0
78	419.0
79	325.5
80	232.0
81	165.5
82	99.0
83	56.0
84	13.0
85	10.0
86	7.0
87	4.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	893747.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.49392140324933
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.7992312467064	16.116811985510537
2	12.765780186333622	6.253680330704267
3	6.065356421828312	4.456930904368688
4	3.4392908826168025	3.3696688224691185
5	2.191796326081664	2.684284347148746
6	1.450435068405348	2.1316105539622296
7	1.0726062880441942	1.8390633881189853
8	0.7542902609662332	1.4780421093874672
9	0.5949655648570035	1.3115735804922557
>10	4.181722419229314	22.437489089018246
>50	1.4495395798385573	25.082310592991274
>100	0.22346684552919963	8.173013257584728
>500	0.006911345738016483	1.125300200104632
>1k	0.003686051060275458	2.020484352870647
>5k	9.215127650688645E-4	1.519736485268175
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGC	7186	0.8040306708721819	No Hit
CCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGC	6280	0.7026597012353608	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3989	0.44632317646940356	No Hit
CTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGCT	3723	0.41656083880561273	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGC	2516	0.28151143444397575	No Hit
ACTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGC	1953	0.21851821600520058	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTG	1624	0.18170690363156464	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTG	1518	0.16984672396103148	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTG	1354	0.15149701201794244	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTG	1226	0.13717528562333636	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2377697491572E-4	0.0	0.0	0.4390504247846426	0.0
2	2.2377697491572E-4	0.0	0.0	2.561463143372789	0.0
3	2.2377697491572E-4	0.0	0.0	3.6349212920434977	0.0
4	2.2377697491572E-4	0.0	0.0	4.772939097977392	0.0
5	2.2377697491572E-4	0.0	0.0	7.717284645430977	0.0
6	2.2377697491572E-4	0.0	0.0	9.176310521881472	0.0
7	2.2377697491572E-4	0.0	0.0	10.731672386033184	0.0
8	2.2377697491572E-4	0.0	0.0	13.06947044297771	0.0
9	2.2377697491572E-4	0.0	0.0	13.872717894437688	0.0
10	2.2377697491572E-4	0.0	0.0	15.464779182475578	0.0
11	2.2377697491572E-4	0.0	0.0	18.094829968660033	0.0
12	2.2377697491572E-4	0.0	0.0	20.100319217854718	0.0
13	2.2377697491572E-4	0.0	0.0	20.964657783466684	0.0
14	2.2377697491572E-4	0.0	0.0	21.291931609280926	0.0
15	2.2377697491572E-4	0.0	0.0	21.893779783316756	0.0
16	2.2377697491572E-4	0.0	0.0	23.15263715570514	0.0
17	2.2377697491572E-4	0.0	0.0	24.628781970736686	0.0
18	2.2377697491572E-4	0.0	0.0	26.30319318554356	0.0
19	2.2377697491572E-4	0.0	0.0	27.265993620118444	0.0
20	2.2377697491572E-4	0.0	0.0	28.17452813827627	0.0
21	2.2377697491572E-4	0.0	0.0	29.35920344348009	0.0
22	2.2377697491572E-4	0.0	0.0	30.480326087807846	0.0
23	2.2377697491572E-4	0.0	0.0	31.520441467216113	0.0
24	2.2377697491572E-4	0.0	0.0	32.39417866577454	0.0
25	2.2377697491572E-4	0.0	0.0	33.12190138820046	0.0
26	3.3566546237358E-4	0.0	0.0	33.854323427099615	0.0
27	3.3566546237358E-4	0.0	0.0	34.53393409991866	0.0
28	3.3566546237358E-4	0.0	0.0	35.22193640929704	0.0
29	3.3566546237358E-4	0.0	0.0	35.901770859090995	0.0
30	3.3566546237358E-4	0.0	0.0	36.67805318507363	0.0
31	3.3566546237358E-4	0.0	0.0	37.42613961221688	0.0
32	3.3566546237358E-4	0.0	0.0	38.11660346831933	0.0
33	3.3566546237358E-4	0.0	0.0	38.7730532242346	0.0
34	3.3566546237358E-4	0.0	0.0	39.404999401396594	0.0
35	3.3566546237358E-4	0.0	0.0	40.06491770042305	0.0
36	3.3566546237358E-4	0.0	0.0	40.713087708266436	0.0
37	3.3566546237358E-4	0.0	0.0	41.34850242853962	0.0
38	4.4755394983144E-4	0.0	0.0	41.97530173527855	0.0
39	4.4755394983144E-4	0.0	0.0	42.596730394619506	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCACG	30	2.1649576E-6	45.000004	1
GTCCGTA	30	2.1649576E-6	45.000004	24
CGTACAT	30	2.1649576E-6	45.000004	35
CCAACGG	30	2.1649576E-6	45.000004	2
AGGTACG	25	3.8901067E-5	45.0	1
CTGTCGG	35	1.2115197E-7	45.0	2
ACTGCGA	20	7.0324447E-4	45.0	27
GATCGAC	20	7.0324447E-4	45.0	11
CGACGCA	20	7.0324447E-4	45.0	21
CGACGAA	20	7.0324447E-4	45.0	14
ACGCACG	35	1.2115197E-7	45.0	1
ACCGCGA	20	7.0324447E-4	45.0	13
CGGTTAG	20	7.0324447E-4	45.0	1
CGTTGCC	20	7.0324447E-4	45.0	21
ATTACCG	20	7.0324447E-4	45.0	35
ATAGCGG	55	1.8189894E-12	45.0	2
CCGTGAT	20	7.0324447E-4	45.0	32
CCTACGG	20	7.0324447E-4	45.0	28
ATTCGCG	20	7.0324447E-4	45.0	1
TACGGAC	20	7.0324447E-4	45.0	27
>>END_MODULE