##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934776.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1164127
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00245763563598	33.0	31.0	34.0	30.0	34.0
2	32.17843929399456	33.0	31.0	34.0	30.0	34.0
3	32.18210813768601	34.0	31.0	34.0	30.0	34.0
4	35.7858094520615	37.0	35.0	37.0	35.0	37.0
5	35.66647367512307	37.0	35.0	37.0	33.0	37.0
6	35.60150481863233	37.0	35.0	37.0	33.0	37.0
7	36.00553633753018	37.0	35.0	37.0	35.0	37.0
8	35.869209287302844	37.0	35.0	37.0	35.0	37.0
9	37.6059055412339	39.0	38.0	39.0	35.0	39.0
10	37.23396330469098	39.0	37.0	39.0	34.0	39.0
11	37.09810269841692	39.0	37.0	39.0	34.0	39.0
12	37.05940588956359	39.0	37.0	39.0	33.0	39.0
13	36.98829852756615	39.0	37.0	39.0	33.0	39.0
14	38.05746709766203	40.0	37.0	41.0	33.0	41.0
15	38.12093697680751	40.0	37.0	41.0	33.0	41.0
16	38.23252617626771	40.0	37.0	41.0	34.0	41.0
17	38.22247658545846	40.0	37.0	41.0	34.0	41.0
18	38.21150355588351	40.0	37.0	41.0	34.0	41.0
19	38.1768441072151	40.0	37.0	41.0	34.0	41.0
20	38.10812737785482	40.0	37.0	41.0	34.0	41.0
21	38.03637661526621	40.0	37.0	41.0	34.0	41.0
22	37.976735356193956	40.0	36.0	41.0	34.0	41.0
23	37.8062642649814	39.0	36.0	41.0	33.0	41.0
24	37.54469744280478	39.0	36.0	41.0	33.0	41.0
25	37.494836044520916	39.0	36.0	41.0	33.0	41.0
26	37.677588441810904	39.0	36.0	41.0	33.0	41.0
27	37.75047138327691	39.0	36.0	41.0	33.0	41.0
28	37.73311159349453	39.0	36.0	41.0	33.0	41.0
29	37.74710577110573	40.0	36.0	41.0	33.0	41.0
30	37.58320612785375	39.0	36.0	41.0	33.0	41.0
31	37.46263251346288	39.0	36.0	41.0	33.0	41.0
32	37.21975523289126	39.0	35.0	41.0	32.0	41.0
33	37.07758775460066	39.0	35.0	41.0	31.0	41.0
34	37.074284850364265	39.0	35.0	41.0	31.0	41.0
35	36.843177763250914	39.0	35.0	41.0	31.0	41.0
36	36.80824171245921	39.0	35.0	41.0	31.0	41.0
37	36.75982775075228	39.0	35.0	41.0	31.0	41.0
38	36.635470184954045	39.0	35.0	41.0	31.0	41.0
39	36.77364840777682	39.0	35.0	41.0	31.0	41.0
40	36.68019382764939	39.0	35.0	41.0	31.0	41.0
41	36.476960847055345	39.0	35.0	40.0	30.0	41.0
42	36.36832579263259	39.0	35.0	40.0	30.0	41.0
43	36.38811057556435	39.0	35.0	40.0	30.0	41.0
44	36.42558243215731	39.0	35.0	40.0	30.0	41.0
45	36.404085636704586	39.0	35.0	40.0	30.0	41.0
46	36.28674191046166	39.0	35.0	40.0	30.0	41.0
47	36.055797176768515	39.0	35.0	40.0	29.0	41.0
48	36.0044539813955	38.0	35.0	40.0	29.0	41.0
49	36.029945186392894	39.0	35.0	40.0	29.0	41.0
50	35.922415681450566	38.0	35.0	40.0	29.0	41.0
51	34.82904356655245	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	13.0
10	13.0
11	11.0
12	16.0
13	13.0
14	15.0
15	27.0
16	38.0
17	91.0
18	182.0
19	416.0
20	711.0
21	1184.0
22	1918.0
23	2756.0
24	4298.0
25	6507.0
26	9204.0
27	11038.0
28	12366.0
29	14572.0
30	17608.0
31	23061.0
32	30226.0
33	41432.0
34	70944.0
35	96254.0
36	96717.0
37	156253.0
38	273649.0
39	292435.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.121137985803955	22.602430834436447	25.022441709538562	12.253989470221033
2	31.94934916894806	23.73821756560925	26.20710626933316	18.10532699610953
3	30.343596531993498	23.242738979509966	29.641783070060225	16.771881418436305
4	27.82402607275667	25.378760221178613	28.61062409857344	18.18658960749128
5	23.736499539998643	28.362627101682204	28.3247446369683	19.576128721350848
6	25.188660687364866	31.718102921760256	27.411356321088675	15.6818800697862
7	77.70775868955879	4.68308011067521	11.287600064254159	6.321561135511847
8	76.85080751498762	3.5487537012714245	11.608870853437812	7.991567930303137
9	71.95383321579175	5.638731856575786	14.417241417817817	7.9901935098146515
10	36.5471293080566	27.60085454593872	21.894947887988167	13.957068258016522
11	24.466402720665357	24.156470900511714	30.707130751198108	20.66999562762482
12	23.899969676847974	21.27285081438709	35.09780290294787	19.729376605817063
13	21.308328043246142	25.205239634507233	35.38608760040786	18.100344721838766
14	17.18369215729899	29.58629084283759	31.86997638573798	21.360040614125435
15	15.093456298153038	27.591834911483026	38.38292557427154	18.931783216092402
16	17.649019393932104	26.871209069113593	33.98572492520146	21.49404661175284
17	17.479106661042994	26.80429197158042	29.738593813217975	25.978007554158612
18	18.572114554511664	26.814600125244066	33.91219342906745	20.70109189117682
19	19.263190356378644	28.433495658119774	30.32959462326705	21.973719362234533
20	21.14279627566408	28.683124779341085	30.881424449394267	19.292654495600566
21	20.035185164505247	28.20216350965144	32.296132638449244	19.466518687394075
22	16.8498797811579	26.676814471273325	30.24798840676318	26.2253173408056
23	17.092293194814655	27.251408136741095	30.770869501351655	24.885429167092592
24	20.234819740457873	24.675400536195795	31.57121173205329	23.518567991293047
25	17.390714243377225	27.545018713593965	28.705029605876337	26.359237437152476
26	18.798034922306588	28.651341305544843	30.132537085730338	22.418086686418235
27	20.051935914208673	28.57866882221613	29.478914242174607	21.89048102140059
28	15.94465208692866	27.706856726113216	30.389038309394078	25.959452877564043
29	20.4751715233819	26.413527046447683	29.27610131884236	23.83520011132806
30	23.18527102283514	28.078723369529268	26.756616760886054	21.97938884674954
31	21.604515658514924	28.83869199838162	24.353786141890016	25.20300620121344
32	21.67933567385689	30.324526447715755	26.1902696183492	21.805868260078153
33	21.837394030032804	30.392903867017946	25.290625507354434	22.47907659559481
34	21.90920750055621	25.265026925756384	28.01696034882792	24.808805224859487
35	20.88835668273307	27.38696035741805	27.818012983119537	23.906669976729344
36	23.591412277182815	29.62245528194089	25.746245899287622	21.039886541588675
37	19.772413147362787	28.94082862093225	29.6764871873945	21.61027104431046
38	18.490508338007793	29.835576358936784	27.442624387201743	24.23129091585368
39	24.271836320264025	26.77585864772486	27.449925996046826	21.50237903596429
40	25.474454247689472	23.03966835233613	30.66993549672845	20.815941903245953
41	20.875127885531388	26.516350879242555	28.086196780935413	24.52232445429064
42	21.60168091625742	26.795186435844197	28.723841986312486	22.87929066158589
43	21.653908894819896	26.96973783788195	28.212299860754026	23.16405340654413
44	20.99865392693409	25.934627407490762	26.245160536608118	26.821558128967027
45	23.437047676069707	23.096535000047247	26.418938827121096	27.04747849676195
46	25.779919201255534	23.67636864362737	28.086883991179658	22.45682816393744
47	19.41497791907584	25.596176362201035	32.08533089602767	22.903514822695463
48	19.53360758748831	23.864492447988923	30.942757963692962	25.659142000829803
49	22.11476926486543	22.00223858737062	31.963694682796636	23.91929746496731
50	21.30592280739129	21.03361574810996	30.74123355956867	26.91922788493008
51	20.254147528577207	20.819807460869818	27.23963966130843	31.686405349244538
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	594.0
1	838.0
2	1082.0
3	4532.0
4	7982.0
5	5429.0
6	2876.0
7	2905.0
8	2934.0
9	2968.5
10	3003.0
11	3159.0
12	3315.0
13	3294.5
14	3274.0
15	3238.5
16	3203.0
17	3143.0
18	3083.0
19	3361.0
20	3639.0
21	3764.5
22	3890.0
23	4916.5
24	5943.0
25	6079.0
26	7485.0
27	8755.0
28	10750.0
29	12745.0
30	14053.5
31	15362.0
32	17543.5
33	19725.0
34	22635.0
35	25545.0
36	28606.5
37	31668.0
38	34900.0
39	38132.0
40	43743.5
41	49355.0
42	58718.0
43	68081.0
44	78844.0
45	89607.0
46	112031.0
47	134455.0
48	157962.0
49	181469.0
50	173879.0
51	166289.0
52	132080.0
53	97871.0
54	78543.5
55	59216.0
56	47776.0
57	36336.0
58	31471.0
59	26606.0
60	23087.5
61	19569.0
62	17217.5
63	14866.0
64	11317.0
65	7768.0
66	6224.0
67	4680.0
68	3503.5
69	2327.0
70	1948.0
71	1569.0
72	1090.0
73	611.0
74	454.5
75	206.5
76	115.0
77	77.5
78	40.0
79	27.5
80	15.0
81	12.5
82	10.0
83	5.5
84	1.0
85	2.0
86	3.0
87	3.0
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1164127.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.193019612251163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.23590240822166	19.628190176506198
2	13.06010241766751	7.625276520339528
3	5.84640191950293	5.120223776914556
4	3.3470479299883436	3.9084174345317746
5	2.0728139547782685	3.025584921719494
6	1.4416194801942204	2.525113545522792
7	0.9836497229442744	2.0100993957448337
8	0.7336930007903764	1.7134971329155892
9	0.5471776230589142	1.4376390373209469
>10	3.695051316593926	22.988118734522477
>50	0.8854221523124274	17.729610627274862
>100	0.1384016802528174	6.509559999226562
>500	0.00857617249152925	1.5646909908534092
>1k	0.003253030945062819	2.033563026349153
>5k	8.871902577444052E-4	2.1804146802578734
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	9975	0.8568652732906289	TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	8601	0.7388369138418747	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	6680	0.5738205539429976	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4852	0.4167930131334468	No Hit
TCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	3805	0.32685437241812965	TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	3085	0.2650054504362496	TruSeq Adapter, Index 21 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	2486	0.21355058339854674	TruSeq Adapter, Index 21 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	1571	0.13495091171324092	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC	1536	0.1319443668946773	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC	1466	0.12593127725755007	No Hit
GCCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	1444	0.12404144908588152	No Hit
GGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	1223	0.10505726608866559	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.577038415911666E-4	0.0	0.0	0.6143659583533412	0.0
2	2.577038415911666E-4	0.0	0.0	2.91145210101647	0.0
3	2.577038415911666E-4	0.0	0.0	4.054969947437006	0.0
4	2.577038415911666E-4	0.0	0.0	5.298992292078098	0.0
5	2.577038415911666E-4	0.0	0.0	8.5158234453801	0.0
6	2.577038415911666E-4	0.0	0.0	10.119943958004582	0.0
7	2.577038415911666E-4	0.0	0.0	11.838742680137132	0.0
8	2.577038415911666E-4	0.0	0.0	14.623490392371279	0.0
9	2.577038415911666E-4	0.0	0.0	15.51858173549793	0.0
10	2.577038415911666E-4	0.0	0.0	17.684582524071686	0.0
11	2.577038415911666E-4	0.0	0.0	21.006900449865007	0.0
12	2.577038415911666E-4	0.0	0.0	23.373137123355097	0.0
13	2.577038415911666E-4	0.0	0.0	24.291507713505485	0.0
14	2.577038415911666E-4	0.0	0.0	24.62214174226695	0.0
15	2.577038415911666E-4	0.0	0.0	25.25832662587501	0.0
16	2.577038415911666E-4	0.0	0.0	26.76202854155947	0.0
17	2.577038415911666E-4	0.0	0.0	28.765074600967075	0.0
18	2.577038415911666E-4	0.0	0.0	30.896972581170267	0.0
19	2.577038415911666E-4	0.0	0.0	32.14932735002281	0.0
20	2.577038415911666E-4	0.0	0.0	33.30890873590253	0.0
21	2.577038415911666E-4	0.0	0.0	34.72129759038318	0.0
22	2.577038415911666E-4	0.0	0.0	36.268551455296546	0.0
23	2.577038415911666E-4	0.0	0.0	37.78548216818268	0.0
24	2.577038415911666E-4	0.0	0.0	38.8964434292822	0.0
25	2.577038415911666E-4	0.0	0.0	39.859740389149984	0.0
26	2.577038415911666E-4	0.0	0.0	40.75036486568905	0.0
27	2.577038415911666E-4	0.0	0.0	41.66426858925186	0.0
28	2.577038415911666E-4	0.0	0.0	42.59904632398355	0.0
29	2.577038415911666E-4	0.0	0.0	43.53081751389668	0.0
30	2.577038415911666E-4	0.0	0.0	44.53801002811549	0.0
31	3.4360512212155546E-4	0.0	0.0	45.46445533863573	0.0
32	3.4360512212155546E-4	0.0	0.0	46.34502936535275	0.0
33	3.4360512212155546E-4	0.0	0.0	47.20000481047171	0.0
34	3.4360512212155546E-4	0.0	0.0	47.98754775037431	0.0
35	3.4360512212155546E-4	0.0	0.0	48.79355946559095	0.0
36	3.4360512212155546E-4	0.0	0.0	49.583164036226286	0.0
37	3.4360512212155546E-4	0.0	0.0	50.39458753211634	0.0
38	3.4360512212155546E-4	0.0	0.0	51.17817901311455	0.0
39	3.4360512212155546E-4	0.0	0.0	51.95240725453495	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAGA	25	3.8908358E-5	45.000004	2
TAAAGCG	25	3.8908358E-5	45.000004	1
GCGCAAA	25	3.8908358E-5	45.000004	1
AACGGAC	25	3.8908358E-5	45.000004	4
GTTAACG	35	1.211938E-7	45.000004	1
CTCGGCT	25	3.8908358E-5	45.000004	41
AGCGCGC	25	3.8908358E-5	45.000004	43
AGACCGC	25	3.8908358E-5	45.000004	22
CGCAGCG	25	3.8908358E-5	45.000004	2
CGTCGCA	25	3.8908358E-5	45.000004	37
TTCGCTC	35	1.211938E-7	45.000004	13
GCGTCGC	25	3.8908358E-5	45.000004	40
TATGTCG	25	3.8908358E-5	45.000004	13
TCGCCGA	20	7.033324E-4	45.0	36
AACGTAC	20	7.033324E-4	45.0	13
CCCTCGT	20	7.033324E-4	45.0	21
CTGTACG	45	3.8562575E-10	45.0	1
TCATTCG	20	7.033324E-4	45.0	29
ATTACGA	20	7.033324E-4	45.0	44
ACGATAA	20	7.033324E-4	45.0	17
>>END_MODULE