SRR2934775.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934775.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	568223
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	4759	0.8375232963114834	Illumina PCR Primer Index 5 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	3510	0.6177152280002746	RNA PCR Primer, Index 33 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	2815	0.4954040931113313	Illumina PCR Primer Index 5 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2712	0.4772774069335455	No Hit
CGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	1404	0.2470860912001098	Illumina PCR Primer Index 5 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1394	0.2453262187556646	RNA PCR Primer, Index 33 (96% over 27bp)
ACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1107	0.19481787960008656	RNA PCR Primer, Index 33 (96% over 27bp)
GCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	683	0.12019928795560897	RNA PCR Primer, Index 33 (96% over 26bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	655	0.11527164511116234	No Hit
ACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	631	0.11104795124449379	RNA PCR Primer, Index 33 (96% over 26bp)
GGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	611	0.10752820635560334	Illumina PCR Primer Index 5 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	581	0.10224858902226766	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	571	0.10048871657782243	RNA PCR Primer, Index 33 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCGA	25	3.8883063E-5	45.000004	38
ACCGGCG	100	0.0	45.000004	20
ACACGAG	25	3.8883063E-5	45.000004	35
TACTTTA	25	3.8883063E-5	45.000004	44
CGAGTAG	25	3.8883063E-5	45.000004	27
AACCCTC	25	3.8883063E-5	45.000004	20
CCGGCTA	25	3.8883063E-5	45.000004	24
TAGGTCG	25	3.8883063E-5	45.000004	1
TCCCTAA	25	3.8883063E-5	45.000004	16
ACGGGTC	65	0.0	45.000004	5
GCCGATG	25	3.8883063E-5	45.000004	9
CTATTCG	25	3.8883063E-5	45.000004	44
CACACGA	25	3.8883063E-5	45.000004	34
CACACCG	100	0.0	45.000004	17
TCCTTCG	25	3.8883063E-5	45.000004	35
AGTCGCG	25	3.8883063E-5	45.000004	2
CGAACCG	20	7.030272E-4	45.0	28
TCCGCAC	20	7.030272E-4	45.0	42
CAACGGA	20	7.030272E-4	45.0	14
ACGCATG	20	7.030272E-4	45.0	1