##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934775.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	568223
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21088551501787	33.0	31.0	34.0	30.0	34.0
2	32.40111540715529	34.0	31.0	34.0	30.0	34.0
3	32.3790290079775	34.0	31.0	34.0	30.0	34.0
4	35.94333210728886	37.0	35.0	37.0	35.0	37.0
5	35.839524271280816	37.0	35.0	37.0	35.0	37.0
6	35.754103582572334	37.0	35.0	37.0	35.0	37.0
7	36.04416927861069	37.0	35.0	37.0	35.0	37.0
8	35.84991631806527	37.0	35.0	37.0	35.0	37.0
9	37.48989921210511	39.0	37.0	39.0	35.0	39.0
10	37.36826034848994	39.0	37.0	39.0	34.0	39.0
11	37.26083949435345	39.0	37.0	39.0	34.0	39.0
12	37.06524023138803	39.0	37.0	39.0	34.0	39.0
13	36.9027828862964	39.0	35.0	39.0	33.0	39.0
14	37.96978299012888	40.0	37.0	41.0	33.0	41.0
15	38.06643518477781	40.0	37.0	41.0	33.0	41.0
16	38.19627505398409	40.0	37.0	41.0	34.0	41.0
17	38.17829971683653	40.0	37.0	41.0	34.0	41.0
18	38.16389164113385	40.0	37.0	41.0	34.0	41.0
19	38.13350568350806	40.0	37.0	41.0	34.0	41.0
20	38.03579580552002	40.0	36.0	41.0	34.0	41.0
21	37.96966331880265	40.0	36.0	41.0	34.0	41.0
22	37.89064680591951	40.0	36.0	41.0	34.0	41.0
23	37.745033199993664	39.0	35.0	41.0	33.0	41.0
24	37.51396370791045	39.0	35.0	41.0	33.0	41.0
25	37.43609287198864	39.0	35.0	41.0	33.0	41.0
26	37.53869695524468	39.0	35.0	41.0	33.0	41.0
27	37.62928814919495	39.0	35.0	41.0	33.0	41.0
28	37.56448436617314	39.0	35.0	41.0	33.0	41.0
29	37.434069018677526	39.0	35.0	41.0	33.0	41.0
30	37.35430456000549	39.0	35.0	41.0	33.0	41.0
31	37.19497450824764	39.0	35.0	41.0	33.0	41.0
32	36.96663105858087	39.0	35.0	41.0	32.0	41.0
33	36.925668267563964	39.0	35.0	41.0	32.0	41.0
34	36.82740754950081	39.0	35.0	41.0	31.0	41.0
35	36.746289748919	39.0	35.0	41.0	31.0	41.0
36	36.75385720043011	39.0	35.0	41.0	31.0	41.0
37	36.64984697909095	39.0	35.0	41.0	31.0	41.0
38	36.51043164391445	39.0	35.0	41.0	31.0	41.0
39	36.53890110044824	39.0	35.0	41.0	31.0	41.0
40	36.485105319566436	39.0	35.0	41.0	31.0	41.0
41	36.495317859361556	39.0	35.0	40.0	31.0	41.0
42	36.4495224586122	39.0	35.0	40.0	31.0	41.0
43	36.407496704638845	39.0	35.0	40.0	31.0	41.0
44	36.29855884045524	39.0	35.0	40.0	30.0	41.0
45	36.19374611728142	38.0	35.0	40.0	30.0	41.0
46	36.15308426445251	38.0	35.0	40.0	30.0	41.0
47	36.044537091951575	38.0	35.0	40.0	30.0	41.0
48	36.03736561174046	38.0	35.0	40.0	30.0	41.0
49	36.05151674606625	38.0	35.0	40.0	30.0	41.0
50	35.94032448528131	38.0	35.0	40.0	30.0	41.0
51	34.98184339599066	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	9.0
10	9.0
11	6.0
12	8.0
13	6.0
14	7.0
15	25.0
16	32.0
17	58.0
18	118.0
19	246.0
20	440.0
21	686.0
22	1120.0
23	1564.0
24	2207.0
25	3221.0
26	4602.0
27	5291.0
28	5845.0
29	6874.0
30	8286.0
31	10745.0
32	13876.0
33	20641.0
34	39406.0
35	55520.0
36	44209.0
37	69693.0
38	123275.0
39	150095.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.54789404863935	23.141090733743617	24.394471888677508	13.916543328939518
2	33.130654690148056	25.562499230055806	24.230979738588548	17.07586634120759
3	31.352831546769487	23.614496421299382	28.37917507739039	16.653496954540735
4	29.49669407961311	26.408645901345068	26.310796993433915	17.783863025607904
5	25.582737763166925	28.78464968859057	25.5482442632558	20.084368284986702
6	25.20348525138898	33.3608460058815	25.889659517478176	15.546009225251353
7	76.41718128270062	6.401536016669512	11.452193944982868	5.729088755646991
8	74.87993270247773	5.9024361914248455	11.756123916138558	7.461507189958871
9	68.85254556749727	7.786555630447905	14.630699566895391	8.730199235159436
10	39.27489735543968	25.0245062237889	21.192383976009417	14.508212444762004
11	30.792664147702574	24.3545227841886	25.421005485522407	19.431807582586412
12	30.221761526724546	20.840409487120372	30.62406836752472	18.31376061863036
13	23.9722784892551	26.59044072485626	30.577783722235814	18.859497063652828
14	17.79107850263013	30.598726204324713	29.15263901672407	22.457556276321093
15	16.239222981118328	27.185453598323196	37.18628073837208	19.389042682186393
16	19.050267236630688	25.67108335987808	33.99809581801511	21.280553585476124
17	18.7269786685861	25.294822631255688	29.291492952590797	26.68670574756742
18	19.324631350719702	26.680194219522967	31.035526545036017	22.959647884721317
19	22.34703628680993	27.471960832278874	27.655163553745627	22.525839327165567
20	24.680451160899857	27.668714571567854	28.787465484501684	18.863368783030605
21	22.667861033432295	27.82006360179014	29.591199229879816	19.920876134897743
22	19.565029926630917	26.429588383433966	27.583712732501148	26.421668957433965
23	19.787477803608795	26.689521543478527	28.92543948414619	24.59756116876649
24	23.08741462418804	24.25913769769967	29.911672002013294	22.741775676098996
25	19.89588594618662	26.754812811167444	28.11449026174583	25.2348109809001
26	18.839258530541706	30.33949699325793	27.572625536101143	23.248618940099224
27	22.553293337298914	28.78570561205724	27.893802257212396	20.76719879343145
28	19.700364117608757	27.20551614418987	30.155766310057846	22.93835342814353
29	20.813307451475918	26.507902707211784	30.444385390946866	22.23440445036544
30	25.902154611833733	27.19002926667875	26.069694468544917	20.838121652942597
31	25.090853414944487	29.79059277783546	23.375153768854833	21.74340003836522
32	24.790619175922128	28.5314040438349	26.378024120811723	20.299952659431245
33	26.806201086545244	26.152408473433848	24.69734593636653	22.344044503654377
34	21.636223806498506	27.043079917567574	28.269886998590344	23.05080927734358
35	21.664381765609626	24.99529234121111	28.816679367079473	24.52364652609979
36	25.451627266055755	26.68776167103408	25.42910089876686	22.431510164143305
37	23.517703436854898	27.366896447345496	29.242040536901886	19.87335957889772
38	21.972007468898656	26.89947432610084	28.50131022503489	22.627207979965615
39	24.38215278156639	24.494784618010886	28.808407966590583	22.31465463383214
40	24.439524623255306	25.035241445700017	29.278469896501903	21.246764034542775
41	19.747352711875443	26.0190101421449	28.1282172668125	26.105419879167158
42	21.63217609987628	24.55250843418869	28.056414471079137	25.758900994855892
43	25.470105926722432	22.59060263312115	28.095659626590262	23.84363181356615
44	23.187727353521417	24.26036960841078	27.736469660678992	24.815433377388807
45	23.36283466174372	24.087374147121817	26.943647124456422	25.60614406667805
46	24.235027445210772	25.124994940366722	27.89890588730129	22.741071727121216
47	20.13681248383117	24.698225872588754	31.51421184992512	23.650749793654956
48	20.72091414814254	22.034834915165348	31.460711727613983	25.78353920907813
49	22.14341904498762	21.0515941804538	32.42371392921441	24.38127284534417
50	21.06479322378714	21.536967000631794	31.484821980102883	25.913417795478182
51	19.195632700541864	21.928186645031968	27.76814736467901	31.108033289747162
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	529.0
1	692.0
2	855.0
3	2794.5
4	4734.0
5	3226.5
6	1719.0
7	1651.5
8	1584.0
9	1547.5
10	1511.0
11	1573.0
12	1635.0
13	1643.0
14	1651.0
15	1599.0
16	1547.0
17	1473.5
18	1400.0
19	1482.0
20	1564.0
21	1748.0
22	1932.0
23	1983.0
24	2034.0
25	2568.5
26	3636.0
27	4169.0
28	5225.0
29	6281.0
30	7251.0
31	8221.0
32	8956.0
33	9691.0
34	10266.0
35	10841.0
36	12328.5
37	13816.0
38	15662.5
39	17509.0
40	19687.0
41	21865.0
42	26610.0
43	31355.0
44	36684.0
45	42013.0
46	49799.0
47	57585.0
48	64821.0
49	72057.0
50	70448.5
51	68840.0
52	55905.5
53	42971.0
54	35517.5
55	28064.0
56	25242.5
57	22421.0
58	21292.0
59	20163.0
60	19952.5
61	19742.0
62	17638.0
63	15534.0
64	12992.0
65	10450.0
66	9039.0
67	7628.0
68	5753.0
69	3878.0
70	3349.5
71	2821.0
72	2440.5
73	2060.0
74	1557.0
75	937.0
76	820.0
77	604.0
78	388.0
79	242.5
80	97.0
81	70.5
82	44.0
83	39.0
84	34.0
85	22.0
86	10.0
87	6.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	568223.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.045918516754853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.28826194022268	18.469187680860742
2	12.596302099364387	6.813571203836746
3	5.562587338425339	4.513358516921519
4	2.996118066745969	3.241310603991542
5	1.7783445339623494	2.404848068013105
6	1.1710762840708266	1.900370025353018
7	0.8492040371407991	1.607725223482652
8	0.6035761261032269	1.3059416580197138
9	0.45224160384405404	1.1008160610707534
>10	4.397303314018121	28.472114495811734
>50	1.1898776425851658	21.383242122935485
>100	0.1058984524357168	4.877722446308833
>500	0.004604280540683339	0.7608599475860855
>1k	0.004604280540683339	3.148931945808113
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	4759	0.8375232963114834	Illumina PCR Primer Index 5 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	3510	0.6177152280002746	RNA PCR Primer, Index 33 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	2815	0.4954040931113313	Illumina PCR Primer Index 5 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2712	0.4772774069335455	No Hit
CGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	1404	0.2470860912001098	Illumina PCR Primer Index 5 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1394	0.2453262187556646	RNA PCR Primer, Index 33 (96% over 27bp)
ACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1107	0.19481787960008656	RNA PCR Primer, Index 33 (96% over 27bp)
GCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	683	0.12019928795560897	RNA PCR Primer, Index 33 (96% over 26bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	655	0.11527164511116234	No Hit
ACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	631	0.11104795124449379	RNA PCR Primer, Index 33 (96% over 26bp)
GGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	611	0.10752820635560334	Illumina PCR Primer Index 5 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	581	0.10224858902226766	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	571	0.10048871657782243	RNA PCR Primer, Index 33 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5218021797780097	0.0
2	0.0	0.0	0.0	2.5416077842677964	0.0
3	0.0	0.0	0.0	3.5339998556904595	0.0
4	0.0	0.0	0.0	4.539590970446462	0.0
5	0.0	0.0	0.0	7.249266573158778	0.0
6	0.0	0.0	0.0	8.599792687026044	0.0
7	0.0	0.0	0.0	10.033912742004459	0.0
8	0.0	0.0	0.0	12.076772675516478	0.0
9	0.0	0.0	0.0	12.788289104805683	0.0
10	0.0	0.0	0.0	14.36407889156194	0.0
11	0.0	0.0	0.0	16.89618336462973	0.0
12	0.0	0.0	0.0	18.71289968903054	0.0
13	0.0	0.0	0.0	19.44447866418642	0.0
14	0.0	0.0	0.0	19.727642140497657	0.0
15	0.0	0.0	0.0	20.27637036867568	0.0
16	0.0	0.0	0.0	21.472027707431764	0.0
17	0.0	0.0	0.0	22.841736430943484	0.0
18	1.7598724444452266E-4	0.0	0.0	24.422101886055298	0.0
19	1.7598724444452266E-4	0.0	0.0	25.226011618677877	0.0
20	1.7598724444452266E-4	0.0	0.0	26.06652669814492	0.0
21	1.7598724444452266E-4	0.0	0.0	27.065254310367585	0.0
22	1.7598724444452266E-4	0.0	0.0	28.04462332570135	0.0
23	1.7598724444452266E-4	0.0	0.0	29.03490355019068	0.0
24	1.7598724444452266E-4	0.0	0.0	29.77299405339101	0.0
25	1.7598724444452266E-4	0.0	0.0	30.44033768432464	0.0
26	1.7598724444452266E-4	0.0	0.0	31.026199221080457	0.0
27	1.7598724444452266E-4	0.0	0.0	31.60607719152516	0.0
28	1.7598724444452266E-4	0.0	0.0	32.19017885583653	0.0
29	1.7598724444452266E-4	0.0	0.0	32.74506663757011	0.0
30	1.7598724444452266E-4	0.0	0.0	33.41892179654818	0.0
31	1.7598724444452266E-4	0.0	0.0	34.03311727965957	0.0
32	1.7598724444452266E-4	0.0	0.0	34.652768367348735	0.0
33	3.519744888890453E-4	0.0	0.0	35.21680748579343	0.0
34	3.519744888890453E-4	0.0	0.0	35.784718323615905	0.0
35	3.519744888890453E-4	0.0	0.0	36.40067367917173	0.0
36	3.519744888890453E-4	0.0	0.0	36.977911840949766	0.0
37	3.519744888890453E-4	0.0	0.0	37.571164841972255	0.0
38	3.519744888890453E-4	0.0	0.0	38.11338154210583	0.0
39	3.519744888890453E-4	0.0	0.0	38.65207849735051	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCGA	25	3.8883063E-5	45.000004	38
ACCGGCG	100	0.0	45.000004	20
ACACGAG	25	3.8883063E-5	45.000004	35
TACTTTA	25	3.8883063E-5	45.000004	44
CGAGTAG	25	3.8883063E-5	45.000004	27
AACCCTC	25	3.8883063E-5	45.000004	20
CCGGCTA	25	3.8883063E-5	45.000004	24
TAGGTCG	25	3.8883063E-5	45.000004	1
TCCCTAA	25	3.8883063E-5	45.000004	16
ACGGGTC	65	0.0	45.000004	5
GCCGATG	25	3.8883063E-5	45.000004	9
CTATTCG	25	3.8883063E-5	45.000004	44
CACACGA	25	3.8883063E-5	45.000004	34
CACACCG	100	0.0	45.000004	17
TCCTTCG	25	3.8883063E-5	45.000004	35
AGTCGCG	25	3.8883063E-5	45.000004	2
CGAACCG	20	7.030272E-4	45.0	28
TCCGCAC	20	7.030272E-4	45.0	42
CAACGGA	20	7.030272E-4	45.0	14
ACGCATG	20	7.030272E-4	45.0	1
>>END_MODULE