##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934774.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 939151 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.096481822411945 33.0 31.0 34.0 30.0 34.0 2 32.26786107878286 34.0 31.0 34.0 30.0 34.0 3 32.27662750718468 34.0 31.0 34.0 30.0 34.0 4 35.85957103809717 37.0 35.0 37.0 35.0 37.0 5 35.74541474161237 37.0 35.0 37.0 35.0 37.0 6 35.65957018626398 37.0 35.0 37.0 33.0 37.0 7 35.999353671560804 37.0 35.0 37.0 35.0 37.0 8 35.810293552368044 37.0 35.0 37.0 35.0 37.0 9 37.47227868574915 39.0 37.0 39.0 35.0 39.0 10 37.28513199687803 39.0 37.0 39.0 34.0 39.0 11 37.16302809665326 39.0 37.0 39.0 34.0 39.0 12 36.927217241955766 39.0 35.0 39.0 33.0 39.0 13 36.7462953241811 39.0 35.0 39.0 33.0 39.0 14 37.773361259265016 40.0 36.0 41.0 33.0 41.0 15 37.90267273313876 40.0 36.0 41.0 33.0 41.0 16 38.021247914339654 40.0 36.0 41.0 33.0 41.0 17 38.00120427918407 40.0 36.0 41.0 33.0 41.0 18 37.98737263762696 40.0 36.0 41.0 34.0 41.0 19 37.95547148435129 40.0 36.0 41.0 34.0 41.0 20 37.87407562788093 40.0 36.0 41.0 34.0 41.0 21 37.786971424190575 39.0 35.0 41.0 33.0 41.0 22 37.7466381870434 39.0 35.0 41.0 33.0 41.0 23 37.555956390399416 39.0 35.0 41.0 33.0 41.0 24 37.29895618489466 39.0 35.0 41.0 33.0 41.0 25 37.21091389989469 39.0 35.0 41.0 32.0 41.0 26 37.38037866115246 39.0 35.0 41.0 33.0 41.0 27 37.4649646329504 39.0 35.0 41.0 33.0 41.0 28 37.40293520424298 39.0 35.0 41.0 33.0 41.0 29 37.45278341821496 39.0 35.0 41.0 33.0 41.0 30 37.31983568137605 39.0 35.0 41.0 33.0 41.0 31 37.154829202119785 39.0 35.0 41.0 32.0 41.0 32 37.073094741953106 39.0 35.0 41.0 32.0 41.0 33 36.945934146905024 39.0 35.0 41.0 32.0 41.0 34 36.843294635260996 39.0 35.0 41.0 31.0 41.0 35 36.760165298232124 39.0 35.0 41.0 31.0 41.0 36 36.70804481920373 39.0 35.0 41.0 31.0 41.0 37 36.62364305633492 39.0 35.0 41.0 31.0 41.0 38 36.51984931070722 39.0 35.0 41.0 31.0 41.0 39 36.589515424037245 39.0 35.0 41.0 31.0 41.0 40 36.47956292438596 39.0 35.0 41.0 31.0 41.0 41 36.404303461317724 39.0 35.0 41.0 31.0 41.0 42 36.30999807272739 39.0 35.0 40.0 31.0 41.0 43 36.23529017165504 39.0 35.0 40.0 30.0 41.0 44 36.153000955117975 38.0 35.0 40.0 30.0 41.0 45 36.09786392177616 38.0 35.0 40.0 30.0 41.0 46 36.013004298563274 38.0 35.0 40.0 30.0 41.0 47 35.887417465349024 38.0 35.0 40.0 30.0 41.0 48 35.835503555871206 38.0 35.0 40.0 29.0 41.0 49 35.853288768259844 38.0 35.0 40.0 30.0 41.0 50 35.72976017701094 38.0 35.0 40.0 29.0 41.0 51 34.755763450180005 37.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 13.0 10 13.0 11 15.0 12 12.0 13 15.0 14 18.0 15 34.0 16 62.0 17 121.0 18 211.0 19 445.0 20 718.0 21 1224.0 22 1938.0 23 2771.0 24 4045.0 25 5780.0 26 7861.0 27 9567.0 28 11044.0 29 12517.0 30 14884.0 31 19167.0 32 25123.0 33 35792.0 34 68502.0 35 90459.0 36 72530.0 37 109867.0 38 196407.0 39 247855.0 40 137.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.730775988099886 20.855006276945883 24.39607688220531 13.01814085274892 2 32.732329518895256 24.073658016655468 24.0972963879078 19.096716076541473 3 31.483329091913863 23.854843363846708 27.669565384054323 16.992262160185103 4 29.09021020048959 24.999387744888736 27.019829612064513 18.89057244255716 5 24.486477680373017 29.215536159786872 26.064285721891366 20.23370043794874 6 25.46896079544184 33.177092927548394 26.270748793325033 15.083197483684732 7 76.28176938532782 6.284186462027938 11.414990773581671 6.019053379062579 8 75.59295576536681 5.311712387038932 11.713238872130253 7.382092975464009 9 69.83658644882452 7.345038231338731 14.395235696922008 8.42313962291474 10 39.700325080844294 23.562132181086962 21.153893250393175 15.583649487675572 11 30.67238388714914 22.81784292408782 26.643851734172674 19.865921454590367 12 28.58571198880691 20.179502550708033 31.289856476754004 19.94492898373105 13 23.393149770377715 25.07381667058865 32.411720798891764 19.121312760141873 14 18.09772869325593 28.935495995851575 30.921971014245848 22.044804296646653 15 17.96942131776466 24.34826774395172 37.96737691808879 19.714934020194836 16 20.296310178022487 24.778017592485128 32.90258967940193 22.023082550090454 17 18.972667867041615 24.375419927146964 29.127264944614872 27.52464726119655 18 21.263566774672018 24.595086413154007 32.4534606256076 21.687886186566377 19 22.010411531266005 26.247536338671846 29.067423662435537 22.67462846762661 20 23.639968439580002 27.25994009483033 28.884598962254206 20.215492503335458 21 22.25510061747259 27.669352425754752 30.47742056389228 19.598126392880378 22 20.402469890358418 24.876084889437376 28.33665725745913 26.384787962745076 23 19.835681376051348 25.775088351074533 30.190991650969867 24.198238621904252 24 22.604565187067895 23.587261260436286 30.403949950540436 23.404223601955383 25 20.116679852334716 25.690969822744158 28.251154500181546 25.941195824739577 26 19.833551793055644 25.94162174133872 29.86090628663548 24.363920178970154 27 20.984271964785215 26.430893434602105 28.2341178362159 24.35071676439678 28 17.513903514983216 27.835459899419796 29.605995202049513 25.04464138354748 29 22.575176941727157 23.431375785150628 28.84903492622592 25.144412346896296 30 22.02883242417886 24.29332450266251 28.081000818824663 25.596842254333968 31 22.77993634676426 28.178216282578628 25.84206373628948 23.199783634367634 32 25.492812124993748 26.777483067153206 25.1421762847508 22.58752852310225 33 22.463373834452607 25.31861223594502 26.236356027944392 25.98165790165799 34 20.25276020576031 25.092131084351717 30.402352763293656 24.252755946594316 35 21.2788997722411 25.176994966730586 27.673718070895948 25.870387190132366 36 21.470881679303965 26.507132505848368 28.224108796136083 23.79787701871158 37 20.402789327807774 27.745910934450368 29.791375401825693 22.05992433591616 38 20.423020366266982 29.596305599419047 25.802134055119996 24.178539979193975 39 22.57986202431771 23.402839373008174 29.87155420161401 24.145744401060107 40 22.602648562371762 22.64055513969532 30.56590473736385 24.190891560569067 41 18.940191726357103 25.47321996143325 28.433020888014816 27.15356742419483 42 21.62389221754542 23.948864453107117 29.08339553490333 25.34384779444413 43 22.636402452853694 23.112683689843276 29.005878713859644 25.245035143443385 44 21.692464790007147 23.535938310239782 27.691606568059875 27.0799903316932 45 22.38628293000806 22.740219623894347 26.757464986993572 28.11603245910402 46 22.906859493308318 24.34869366055086 28.138925476307858 24.605521369832967 47 18.683576975374567 23.542007621777543 32.345277809425745 25.429137593422148 48 19.359719576511125 23.34427584062627 31.269306000845443 26.02669858201716 49 21.986773160013673 20.13510074524757 33.200518340501155 24.677607754237606 50 20.317606007979546 20.599243359161626 31.893380297737 27.18977033512183 51 18.98608423991456 21.584281973825295 28.32079186414112 31.10884192211902 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 768.0 1 1099.0 2 1430.0 3 4852.0 4 8274.0 5 5444.0 6 2614.0 7 2655.5 8 2697.0 9 2709.0 10 2721.0 11 2707.0 12 2693.0 13 2585.0 14 2477.0 15 2458.0 16 2439.0 17 2449.5 18 2460.0 19 2328.5 20 2197.0 21 2439.0 22 2681.0 23 3234.5 24 3788.0 25 3719.0 26 4692.5 27 5735.0 28 6764.0 29 7793.0 30 9466.0 31 11139.0 32 12086.0 33 13033.0 34 15025.5 35 17018.0 36 19351.5 37 21685.0 38 23582.0 39 25479.0 40 31008.5 41 36538.0 42 43709.0 43 50880.0 44 59285.0 45 67690.0 46 78476.5 47 89263.0 48 104310.5 49 119358.0 50 116666.0 51 113974.0 52 96387.5 53 78801.0 54 66957.5 55 55114.0 56 49053.5 57 42993.0 58 39623.5 59 36254.0 60 33480.0 61 30706.0 62 27300.5 63 23895.0 64 21295.0 65 18695.0 66 15619.0 67 12543.0 68 9851.0 69 7159.0 70 6161.5 71 5164.0 72 4376.5 73 3589.0 74 2812.5 75 1532.0 76 1028.0 77 727.5 78 427.0 79 289.5 80 152.0 81 116.5 82 81.0 83 51.0 84 21.0 85 14.5 86 8.0 87 4.5 88 1.0 89 3.0 90 5.0 91 3.5 92 2.0 93 2.0 94 2.0 95 1.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 939151.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.97932301059467 #Duplication Level Percentage of deduplicated Percentage of total 1 66.3375783831296 16.57067798172837 2 12.601731468751328 6.2956544170143 3 5.964521944227527 4.469691607459186 4 3.2927072332535245 3.289983902350451 5 2.1921956630372956 2.737978178471668 6 1.5114679786264278 2.265326811496809 7 1.0372381610126742 1.8136654943995558 8 0.7324176953369184 1.4636238552396963 9 0.5764119326183906 1.2958541866832318 >10 4.098031206923893 22.37566758506272 >50 1.410617076651203 24.719715482377243 >100 0.23517463270897693 8.625943922813551 >500 0.005599396016880403 0.9798369938141643 >1k 0.0034457821642340943 1.9062009464593774 >5k 8.614455410585236E-4 1.190178634629657 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC 6040 0.6431340647031202 No Hit CCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC 5022 0.5347382902217002 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4732 0.5038593367839677 No Hit CTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGCT 3832 0.40802810197721134 No Hit TCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC 2351 0.25033248114520457 No Hit CGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTG 1789 0.1904911989658745 No Hit ACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC 1704 0.1814404712341253 No Hit GCCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTG 1240 0.13203414573375313 No Hit TTATTAGGGCGATCTGAGTGGGACTTGACTTAATGAAGGAAAAGAGGGAAA 1037 0.11041887832734032 No Hit ACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTG 1032 0.10988648257841392 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.43177295237932983 0.0 2 0.0 0.0 0.0 2.14800388861855 0.0 3 0.0 0.0 0.0 3.0899184476191794 0.0 4 0.0 0.0 0.0 4.033749631315944 0.0 5 0.0 0.0 0.0 6.589781621911706 0.0 6 0.0 0.0 0.0 7.915234078438931 0.0 7 0.0 0.0 0.0 9.34737864305101 0.0 8 0.0 0.0 0.0 11.532330796645056 0.0 9 0.0 0.0 0.0 12.263097201621465 0.0 10 0.0 0.0 0.0 13.930347728959454 0.0 11 0.0 0.0 0.0 16.372021112685818 0.0 12 0.0 0.0 0.0 18.306747264284443 0.0 13 0.0 0.0 0.0 19.06807318524923 0.0 14 0.0 0.0 0.0 19.334271059712442 0.0 15 1.064791497852848E-4 0.0 0.0 19.91042973920062 0.0 16 1.064791497852848E-4 0.0 0.0 21.150485917600044 0.0 17 1.064791497852848E-4 0.0 0.0 22.590084022697095 0.0 18 1.064791497852848E-4 0.0 0.0 24.379572613988593 0.0 19 2.129582995705696E-4 0.0 0.0 25.32351027683514 0.0 20 2.129582995705696E-4 0.0 0.0 26.16874176783073 0.0 21 2.129582995705696E-4 0.0 0.0 27.246736680256955 0.0 22 2.129582995705696E-4 0.0 0.0 28.33197217486858 0.0 23 2.129582995705696E-4 0.0 0.0 29.397296068470354 0.0 24 2.129582995705696E-4 0.0 0.0 30.22368074995395 0.0 25 2.129582995705696E-4 0.0 0.0 30.98532610836809 0.0 26 2.129582995705696E-4 0.0 0.0 31.664450125698636 0.0 27 2.129582995705696E-4 0.0 0.0 32.33292622805065 0.0 28 2.129582995705696E-4 0.0 0.0 33.01811955691896 0.0 29 2.129582995705696E-4 0.0 0.0 33.703419364937055 0.0 30 2.129582995705696E-4 0.0 0.0 34.52256346423525 0.0 31 2.129582995705696E-4 0.0 0.0 35.24566337042712 0.0 32 2.129582995705696E-4 0.0 0.0 35.9129682021315 0.0 33 2.129582995705696E-4 0.0 0.0 36.577611055091246 0.0 34 2.129582995705696E-4 0.0 0.0 37.246938990641546 0.0 35 2.129582995705696E-4 0.0 0.0 37.945442213233015 0.0 36 2.129582995705696E-4 0.0 0.0 38.59368727712583 0.0 37 2.129582995705696E-4 0.0 0.0 39.251621943649106 0.0 38 3.194374493558544E-4 0.0 0.0 39.95001868709079 0.0 39 3.194374493558544E-4 0.0 0.0 40.64511457688913 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGTAA 20 7.032626E-4 45.000004 32 ACTATCG 20 7.032626E-4 45.000004 25 TCGTTGC 30 2.1650758E-6 45.000004 33 GATCGGC 20 7.032626E-4 45.000004 10 CGAAATT 20 7.032626E-4 45.000004 14 CACGCGA 20 7.032626E-4 45.000004 14 CGCGAAA 30 2.1650758E-6 45.000004 28 TCGATAG 30 2.1650758E-6 45.000004 1 CCGCTAG 30 2.1650758E-6 45.000004 3 TGGCACG 40 6.8121153E-9 45.000004 1 ACGATAG 40 6.8121153E-9 45.000004 1 CGGATAC 20 7.032626E-4 45.000004 10 AATTCGT 20 7.032626E-4 45.000004 19 TCCGTCA 20 7.032626E-4 45.000004 28 CCGACCA 20 7.032626E-4 45.000004 23 CATAAGT 30 2.1650758E-6 45.000004 12 TTACGAC 20 7.032626E-4 45.000004 42 CGGTCTA 40 6.8121153E-9 45.000004 31 ACGTAAG 20 7.032626E-4 45.000004 1 GCTACGA 80 0.0 45.000004 10 >>END_MODULE