FastQCFastQC Report
Sat 14 Jan 2017
SRR2934773.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934773.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2669802
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC80530.30163285517053323TruSeq Adapter, Index 16 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69920.2618920803864856No Hit
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC65730.24619803266309634TruSeq Adapter, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT54780.20518375519982382TruSeq Adapter, Index 27 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG37270.1395983672197414TruSeq Adapter, Index 27 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC30340.1136413861402456TruSeq Adapter, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG207.034967E-445.010
CGTTTTT44350.040.7891771
CCGCGTA451.9299478E-840.043
ACGCGAA403.4605182E-739.3751
CCGTACA403.4605182E-739.37526
CGTTAGG4500.039.02
ACGCATT301.14032635E-437.50000413
TAGCCGT301.14032635E-437.50000444
ACCTACG301.14032635E-437.50000440
AGTCGTC1200.037.50000435
TACGAAT2050.037.3170712
AGGGCGA18650.037.278826
CGTAAGG4350.037.241382
TTACCGG1400.036.9642832
TACGGGA11650.036.8884124
CGAAAGG3300.036.818182
CGAATAT2050.036.2195114
CGCGACA250.002107606336.00000427
CGACACG250.002107606336.00000429
TACGCGC250.002107606336.00000434
Produced by FastQC (version 0.11.5)