##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934772.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	299560
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.780568166644414	34.0	31.0	34.0	31.0	34.0
2	32.97710308452397	34.0	31.0	34.0	31.0	34.0
3	32.93652022967018	34.0	31.0	34.0	31.0	34.0
4	36.348264120710375	37.0	37.0	37.0	35.0	37.0
5	36.24492255307785	37.0	37.0	37.0	35.0	37.0
6	36.22813125918013	37.0	37.0	37.0	35.0	37.0
7	36.32451929496595	37.0	37.0	37.0	35.0	37.0
8	35.792121778608625	37.0	37.0	37.0	35.0	37.0
9	37.78265456002136	39.0	38.0	39.0	35.0	39.0
10	37.724759647482976	39.0	37.0	39.0	35.0	39.0
11	37.689584724262254	39.0	37.0	39.0	35.0	39.0
12	37.55366203765523	39.0	37.0	39.0	35.0	39.0
13	37.482854853785554	39.0	37.0	39.0	35.0	39.0
14	38.63826278541861	40.0	38.0	41.0	35.0	41.0
15	38.77837828815596	40.0	38.0	41.0	35.0	41.0
16	38.89520296434771	40.0	38.0	41.0	35.0	41.0
17	38.88317532380825	40.0	38.0	41.0	35.0	41.0
18	38.78387635198291	40.0	38.0	41.0	35.0	41.0
19	38.765412605154225	40.0	38.0	41.0	35.0	41.0
20	38.71102617171852	40.0	38.0	41.0	35.0	41.0
21	38.679726932834825	40.0	38.0	41.0	35.0	41.0
22	38.646117639204164	40.0	38.0	41.0	35.0	41.0
23	38.49814060622246	40.0	37.0	41.0	35.0	41.0
24	38.32424556015489	40.0	37.0	41.0	34.0	41.0
25	38.259250233676056	40.0	37.0	41.0	34.0	41.0
26	38.35400587528375	40.0	37.0	41.0	35.0	41.0
27	38.39391440779811	40.0	37.0	41.0	35.0	41.0
28	38.32232274001869	40.0	37.0	41.0	35.0	41.0
29	38.290082120443316	40.0	37.0	41.0	35.0	41.0
30	38.194959273601285	40.0	37.0	41.0	34.0	41.0
31	38.07773400988116	40.0	37.0	41.0	34.0	41.0
32	37.99427159834424	40.0	37.0	41.0	34.0	41.0
33	37.88251435438643	40.0	37.0	41.0	34.0	41.0
34	37.77535719054613	40.0	36.0	41.0	34.0	41.0
35	37.65465683001736	40.0	36.0	41.0	34.0	41.0
36	37.590796501535586	40.0	36.0	41.0	33.0	41.0
37	37.55060088129256	40.0	36.0	41.0	33.0	41.0
38	37.47529042595807	40.0	36.0	41.0	33.0	41.0
39	37.4646347977033	40.0	36.0	41.0	33.0	41.0
40	37.36385699025237	40.0	36.0	41.0	33.0	41.0
41	37.285592201896115	40.0	35.0	41.0	33.0	41.0
42	37.24099345707037	40.0	35.0	41.0	33.0	41.0
43	37.185201629055946	40.0	35.0	41.0	33.0	41.0
44	37.126255174255576	40.0	35.0	41.0	33.0	41.0
45	37.047449592736015	40.0	35.0	41.0	33.0	41.0
46	36.99778007744692	40.0	35.0	41.0	33.0	41.0
47	36.8686974228869	39.0	35.0	41.0	33.0	41.0
48	36.76719188142609	39.0	35.0	41.0	32.0	41.0
49	36.75632928294832	39.0	35.0	41.0	32.0	41.0
50	36.67299372412872	39.0	35.0	41.0	32.0	41.0
51	35.920296434771	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	2.0
12	6.0
13	4.0
14	13.0
15	14.0
16	38.0
17	53.0
18	69.0
19	133.0
20	232.0
21	360.0
22	562.0
23	811.0
24	1071.0
25	1477.0
26	1990.0
27	2203.0
28	2325.0
29	2295.0
30	2595.0
31	3220.0
32	4314.0
33	7074.0
34	16919.0
35	20978.0
36	16753.0
37	29027.0
38	62417.0
39	122476.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.94552009614101	21.93350247028976	25.51775938042462	10.603218053144612
2	34.70623581252504	24.36072906930164	23.30818533849646	17.62484977967686
3	32.62618507143811	23.836293230070773	26.068901054880488	17.46862064361063
4	29.354720256376016	25.83756175724396	25.37855521431433	19.4291627720657
5	25.114501268527174	29.852116437441584	25.569835759113367	19.46354653491788
6	26.447790092135133	34.416143677393514	24.70823875016691	14.427827480304448
7	81.40172252637201	6.1483509146748565	8.2985044732274	4.151422085725731
8	82.08939778341568	5.305114167445587	7.962344772332755	4.643143276805982
9	76.83402323407664	7.214247563092535	10.11950861263186	5.8322205901989586
10	41.737214581386034	25.206636400053412	18.411670449993323	14.644478568567232
11	31.7014955267726	25.615569501936175	24.017225263720125	18.665709707571104
12	27.793764187474963	23.287154493256775	28.781880090799838	20.13720122846842
13	26.29523300841234	24.212511683802912	30.343169982641204	19.149085325143545
14	22.00727734009881	26.652757377486978	31.293563893710775	20.04640138870343
15	20.71972225931366	25.763786887434904	33.78722125784484	19.729269595406596
16	25.612231272533048	25.746428094538658	29.728601949525974	18.912738683402324
17	25.294431833355592	25.327814127386834	27.442248631325945	21.935505407931633
18	26.164708238750165	24.544665509413807	30.126852717318737	19.163773534517293
19	25.34483909734277	26.882427560421952	27.997396181065564	19.775337161169716
20	25.53211376685806	27.976699158766188	27.782414207504342	18.708772866871413
21	24.321004139404458	26.891106956870075	30.06776605688343	18.720122846842035
22	23.491454132728002	24.552343437040992	30.05808519161437	21.89811723861664
23	22.049672853518494	25.879623447723326	30.344839097342767	21.72586460141541
24	22.71064227533716	25.952730671651757	29.675857924956606	21.66076912805448
25	22.803445052744024	26.68413673387635	28.025103485111497	22.487314728268124
26	21.248497796768596	27.65889971958873	28.40699692882895	22.685605554813726
27	20.763786887434904	26.52256643076512	30.373214047269325	22.340432634530643
28	19.6918814260916	27.420883963145947	29.66884764321004	23.21838696755241
29	21.42842836159701	26.869074642809455	30.109493924422488	21.59300307117105
30	23.090198958472428	25.481038856990253	29.95259714247563	21.47616504206169
31	23.162304713579918	27.181199092001602	28.238416343971156	21.41807985044732
32	23.86900787822139	26.576311924155426	27.856189077313392	21.698491120309786
33	23.808585926024836	25.68433702764054	27.64721591667779	22.85986112965683
34	21.448457738015755	25.934704232874882	29.724929897182534	22.891908131926826
35	21.4791694485245	26.903458405661635	29.34871144345039	22.268660702363466
36	23.031112298037122	27.314394445186274	29.19181466150354	20.46267859527307
37	21.356322606489517	28.62898918413673	27.701962878889034	22.31272533048471
38	22.25731072239284	28.616971558285485	26.218453732140475	22.9072639871812
39	22.802443583923086	25.68433702764054	27.283682734677527	24.229536653758846
40	24.320336493523833	24.45653625317132	28.242088396314596	22.981038856990253
41	21.548604620109494	24.35104820403258	28.32153825610896	25.778808919748965
42	21.347977032981706	25.229336359994658	30.11550273734811	23.307183869675523
43	21.641407397516357	24.883829616771262	30.137535051408733	23.337227934303645
44	21.636066230471357	25.724729603418346	28.04880491387368	24.590399252236615
45	21.72486313259447	24.43650687675257	28.250767792762723	25.58786219789024
46	22.376151689144077	25.05407931633062	28.13025771130992	24.43951128321538
47	19.953932434236883	26.341968220056085	30.576846040859927	23.12725330484711
48	20.936373347576446	25.719054613433034	30.47135799172119	22.87321404726933
49	22.765055414608092	23.836960875951398	30.213980504740285	23.184003204700225
50	20.347175857924956	24.93390305781813	29.369742288690077	25.34917879556683
51	20.576512217919614	24.93356923487782	28.223060488716783	26.26685805848578
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	118.0
1	267.0
2	416.0
3	1057.0
4	1698.0
5	1194.5
6	691.0
7	702.5
8	714.0
9	705.5
10	697.0
11	676.0
12	655.0
13	667.0
14	679.0
15	696.5
16	714.0
17	714.0
18	714.0
19	711.0
20	708.0
21	903.5
22	1099.0
23	1372.5
24	1646.0
25	1747.5
26	2129.5
27	2410.0
28	2938.0
29	3466.0
30	4208.5
31	4951.0
32	5655.0
33	6359.0
34	7341.5
35	8324.0
36	8995.0
37	9666.0
38	10315.5
39	10965.0
40	12623.0
41	14281.0
42	15516.5
43	16752.0
44	18775.0
45	20798.0
46	22420.5
47	24043.0
48	26450.5
49	28858.0
50	28829.0
51	28800.0
52	25997.5
53	23195.0
54	20690.5
55	18186.0
56	16941.0
57	15696.0
58	14629.0
59	13562.0
60	12262.5
61	10963.0
62	9668.5
63	8374.0
64	7134.0
65	5894.0
66	4924.0
67	3954.0
68	3390.5
69	2827.0
70	2362.0
71	1897.0
72	1577.0
73	1257.0
74	983.0
75	569.5
76	430.0
77	358.5
78	287.0
79	211.5
80	136.0
81	107.0
82	78.0
83	48.5
84	19.0
85	14.0
86	9.0
87	7.0
88	5.0
89	5.5
90	6.0
91	5.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	299560.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.669727442609585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.79158238576021	23.824375391606697
2	10.718595033891095	7.64658726853208
3	4.333406080807177	4.63714241401617
4	2.7645921055065785	3.9444898757363926
5	2.1010756056678233	3.747239709524356
6	1.7830037481922387	3.8159554636301074
7	1.5848186476415929	3.9571034445138507
8	1.390685580222688	3.968430048392852
9	1.2582844803606064	4.039439801375561
>10	7.200290975417511	37.6282746543907
>50	0.05176484513092675	1.260904046733667
>100	0.020905033610566573	1.2435049202087587
>500	9.954777909793606E-4	0.2865529613387973
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	807	0.26939511283215384	No Hit
GCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGC	387	0.12918947790092136	No Hit
CCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGC	384	0.12818800907998398	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	302	0.10081452797436238	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07978368273467752	0.0
2	0.0	0.0	0.0	0.43230070770463347	0.0
3	0.0	0.0	0.0	0.724061957537722	0.0
4	0.0	0.0	0.0	1.075243690746428	0.0
5	0.0	0.0	0.0	2.0676992922953668	0.0
6	0.0	0.0	0.0	2.714314327680598	0.0
7	0.0	0.0	0.0	3.3048471090933367	0.0
8	0.0	0.0	0.0	4.192816130324476	0.0
9	0.0	0.0	0.0	4.561690479369743	0.0
10	0.0	0.0	0.0	5.406596341300574	0.0
11	0.0	0.0	0.0	6.750567498998532	0.0
12	0.0	0.0	0.0	7.699959941247163	0.0
13	0.0	0.0	0.0	8.148617973027106	0.0
14	0.0	0.0	0.0	8.305180932033648	0.0
15	0.0	0.0	0.0	8.62298037121111	0.0
16	0.0	0.0	0.0	9.241220456669783	0.0
17	0.0	0.0	0.0	9.984644144745626	0.0
18	0.0	0.0	0.0	10.802844171451461	0.0
19	0.0	0.0	0.0	11.318266791293897	0.0
20	0.0	0.0	0.0	11.836359994658833	0.0
21	0.0	0.0	0.0	12.493323541193751	0.0
22	0.0	0.0	0.0	13.171651755908666	0.0
23	0.0	0.0	0.0	13.827947656562959	0.0
24	0.0	0.0	0.0	14.368740819869142	0.0
25	0.0	0.0	0.0	14.84110028041127	0.0
26	0.0	0.0	0.0	15.27440245693684	0.0
27	0.0	0.0	0.0	15.787822139137402	0.0
28	0.0	0.0	0.0	16.272866871411402	0.0
29	0.0	0.0	0.0	16.801308585926026	0.0
30	0.0	0.0	0.0	17.346107624515955	0.0
31	0.0	0.0	0.0	17.842836159700894	0.0
32	0.0	0.0	0.0	18.34190145546802	0.0
33	0.0	0.0	0.0	18.844638803578583	0.0
34	0.0	0.0	0.0	19.374081986914142	0.0
35	0.0	0.0	0.0	19.920883963145947	0.0
36	0.0	0.0	0.0	20.429630124182133	0.0
37	0.0	0.0	0.0	20.948724796368005	0.0
38	0.0	0.0	0.0	21.537922286019494	0.0
39	0.0	0.0	0.0	22.310054746962212	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATG	20	7.024926E-4	45.000004	1
GGATGCG	20	7.024926E-4	45.000004	1
ATGCGAG	20	7.024926E-4	45.000004	1
ACCCAGT	20	7.024926E-4	45.000004	39
CGTTAGG	35	1.2078999E-7	45.0	2
TATTGCG	25	3.8838778E-5	45.0	1
TTTTACG	35	1.2078999E-7	45.0	1
ATAGCGG	30	2.16011E-6	44.999996	2
CGTTTTT	585	0.0	40.384617	1
ATTGCGG	40	3.4468212E-7	39.375004	2
CACGGGC	70	0.0	38.571426	4
TTGTACG	35	6.2328327E-6	38.571426	1
ATCGAGG	35	6.2328327E-6	38.571426	2
CAGCGGG	185	0.0	37.702705	3
ACACGGG	90	0.0	37.500004	3
CTCGTAC	30	1.1379081E-4	37.499996	29
CGTGCGG	30	1.1379081E-4	37.499996	2
TTTCGCG	30	1.1379081E-4	37.499996	1
ACGGGCC	30	1.1379081E-4	37.499996	5
ACGATGC	30	1.1379081E-4	37.499996	43
>>END_MODULE