##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934771.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	681963
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.253723442474154	33.0	31.0	34.0	30.0	34.0
2	32.42927842126333	34.0	31.0	34.0	31.0	34.0
3	32.439075140440174	34.0	31.0	34.0	30.0	34.0
4	35.9833685991762	37.0	35.0	37.0	35.0	37.0
5	35.86871575144106	37.0	35.0	37.0	35.0	37.0
6	35.806017335251326	37.0	35.0	37.0	35.0	37.0
7	36.119776292848734	37.0	35.0	37.0	35.0	37.0
8	35.960486126080156	37.0	35.0	37.0	35.0	37.0
9	37.692534638976014	39.0	38.0	39.0	35.0	39.0
10	37.40002023570194	39.0	37.0	39.0	35.0	39.0
11	37.25511647992633	39.0	37.0	39.0	34.0	39.0
12	37.08449138736266	39.0	37.0	39.0	34.0	39.0
13	36.99195117623683	39.0	37.0	39.0	33.0	39.0
14	38.15171644209437	40.0	37.0	41.0	33.0	41.0
15	38.248575069321944	40.0	37.0	41.0	34.0	41.0
16	38.34335440485774	40.0	37.0	41.0	34.0	41.0
17	38.34752325272779	40.0	37.0	41.0	34.0	41.0
18	38.33547861100969	40.0	37.0	41.0	34.0	41.0
19	38.29326664349826	40.0	37.0	41.0	34.0	41.0
20	38.23769324728761	40.0	36.0	41.0	34.0	41.0
21	38.15203757388597	40.0	36.0	41.0	34.0	41.0
22	38.09999956009344	40.0	36.0	41.0	34.0	41.0
23	37.94850600399142	40.0	36.0	41.0	34.0	41.0
24	37.71098871932935	39.0	35.0	41.0	33.0	41.0
25	37.62879950965082	39.0	35.0	41.0	33.0	41.0
26	37.78282546120537	39.0	36.0	41.0	34.0	41.0
27	37.85550682368398	40.0	36.0	41.0	34.0	41.0
28	37.83865400322305	40.0	36.0	41.0	34.0	41.0
29	37.871249613248814	40.0	36.0	41.0	34.0	41.0
30	37.74774437909388	40.0	36.0	41.0	34.0	41.0
31	37.63718999417857	40.0	36.0	41.0	33.0	41.0
32	37.54084019220984	40.0	35.0	41.0	33.0	41.0
33	37.40289722462949	40.0	35.0	41.0	33.0	41.0
34	37.316525969884	40.0	35.0	41.0	33.0	41.0
35	37.25542294816581	40.0	35.0	41.0	33.0	41.0
36	37.15943386957944	39.0	35.0	41.0	33.0	41.0
37	37.0464174742618	39.0	35.0	41.0	32.0	41.0
38	36.9515354938611	39.0	35.0	41.0	32.0	41.0
39	36.98992320697751	39.0	35.0	41.0	32.0	41.0
40	36.88968316462917	39.0	35.0	41.0	31.0	41.0
41	36.87838489771439	39.0	35.0	41.0	32.0	41.0
42	36.766001967848695	39.0	35.0	41.0	31.0	41.0
43	36.659755734548646	39.0	35.0	41.0	31.0	41.0
44	36.577176474383506	39.0	35.0	41.0	31.0	41.0
45	36.54985387770304	39.0	35.0	41.0	31.0	41.0
46	36.52178637257447	39.0	35.0	41.0	31.0	41.0
47	36.4273428323824	39.0	35.0	41.0	31.0	41.0
48	36.375835639176906	39.0	35.0	41.0	31.0	41.0
49	36.336770469952185	39.0	35.0	40.0	31.0	41.0
50	36.20082174546126	38.0	35.0	40.0	31.0	41.0
51	35.25982054744905	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	10.0
11	12.0
12	12.0
13	17.0
14	15.0
15	19.0
16	38.0
17	63.0
18	133.0
19	223.0
20	415.0
21	654.0
22	1070.0
23	1563.0
24	2404.0
25	3555.0
26	4893.0
27	5749.0
28	6350.0
29	7318.0
30	8895.0
31	11311.0
32	15096.0
33	21707.0
34	42706.0
35	64555.0
36	49071.0
37	77688.0
38	144164.0
39	212100.0
40	153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.13032965131539	21.890483794575367	25.99407885765064	11.985107696458606
2	31.161661263147707	24.603387573812654	26.248931393638657	17.98601976940098
3	30.172164765537136	25.074820774734114	27.31790434378405	17.435110115944706
4	26.889875257162043	26.830927777606707	28.03099288377815	18.2482040814531
5	24.6201040232388	29.927576716038846	26.806732916595184	18.645586344127175
6	25.69538230079931	33.7031481180064	25.831166793506394	14.770302787687895
7	81.56263609609319	5.07622847573842	8.674664167997385	4.686471260171007
8	82.6461259628455	3.6685274714317346	8.299863775600729	5.385482790122045
9	77.71242721379312	6.112501704637935	10.055824142952037	6.119246938616905
10	40.666575752643475	29.456583421681238	16.481392685526927	13.395448140148366
11	29.16433882776632	25.631889120084228	27.017448160677336	18.186323891472117
12	25.692156319331104	23.356399100830984	31.17515173110565	19.776292848732265
13	22.397402791647053	27.09883087498882	31.811989800033142	18.691776533330987
14	18.24923053010207	30.894931249935848	30.52511646526278	20.3307217546993
15	16.779649335814405	26.917882641726898	37.5018879323365	18.80058009012219
16	19.510589284169374	26.126050826804388	33.41178333721918	20.951576551807065
17	19.701068826314625	26.0081558676937	28.244787473807232	26.04598783218444
18	21.135457495494624	25.52748462893148	31.698493906560916	21.63856396901298
19	22.333469704368124	27.963393908467175	28.678242074716664	21.024894312448037
20	23.68618238819408	28.03685830462943	28.00826437797945	20.26869492919704
21	22.28258717848329	27.916763812699514	29.758066053436917	20.04258295538028
22	21.20320310632688	25.381728920777224	28.188186162592398	25.226881810303492
23	19.523053303478342	26.996919187697866	29.25305331814189	24.2269741906819
24	20.829575798100482	25.721776694630062	30.792286385038487	22.656361122230972
25	18.963638789787716	28.0842215780035	29.960716343848564	22.99142328836022
26	18.342344086116107	29.324904723570043	30.7450697471857	21.587681443128144
27	20.028066038773364	28.295523364170784	30.10573887439641	21.57067172265944
28	16.876722050903055	27.50251846507802	33.16690201667832	22.453857467340605
29	19.524226387648596	25.251223306836295	32.391053473575546	22.833496831939563
30	20.962134309339366	27.53859080331338	30.53963338186969	20.95964150547757
31	22.410160081998583	28.540404684711635	26.712739547453456	22.33669568583633
32	23.744836596706858	27.9696992358823	27.168776018640305	21.116688148770535
33	23.773870429920684	27.2963489221556	26.624025057077876	22.305755590845838
34	20.62223317100781	26.279724853107865	28.769449368954035	24.32859260693029
35	22.15618735913825	25.1171984403846	29.47124697380943	23.255367226667722
36	24.30454438144005	26.360814296376784	29.106564432381226	20.228076889801937
37	22.028467820101678	27.36482771059427	29.28868574981341	21.31801871949065
38	22.80548944737471	29.600432868058824	25.401378080629005	22.192699603937456
39	23.318860407382804	26.50876953735027	27.31453172679456	22.857838328472365
40	23.275016386519503	24.820261509788654	28.526474310189847	23.378247793501995
41	21.801036126593377	24.605000564546756	27.138275830213665	26.4556874786462
42	23.54159976421008	24.036641284057932	28.788365351199406	23.633393600532578
43	23.977987075545155	24.637113743707502	28.402127388142755	22.982771792604584
44	20.8911627170389	26.468884675561576	28.18671980737958	24.45323280001994
45	21.81540640767901	25.988506707841918	27.00542404793222	25.19066283654685
46	23.27486975099822	25.797440623611546	28.288338223627967	22.639351401762266
47	20.620180273709863	24.304984288003894	30.657235069937812	24.41760036834843
48	22.263231289674074	23.59482845843543	30.380973747842628	23.76096650404787
49	23.44188760973836	22.080083523592922	31.334691178260403	23.143337688408316
50	21.66701126014168	24.14808428023221	29.654394739890584	24.530509719735527
51	21.008031227500613	23.724014352684822	26.735614688773442	28.53233973104113
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	352.0
1	471.0
2	590.0
3	2406.5
4	4223.0
5	2861.5
6	1500.0
7	1477.5
8	1455.0
9	1583.5
10	1712.0
11	1638.0
12	1564.0
13	1566.0
14	1568.0
15	1543.0
16	1518.0
17	1536.0
18	1554.0
19	1553.0
20	1552.0
21	1841.0
22	2130.0
23	2246.0
24	2362.0
25	3256.5
26	4798.5
27	5446.0
28	5794.5
29	6143.0
30	7613.0
31	9083.0
32	10420.0
33	11757.0
34	14345.0
35	16933.0
36	18648.0
37	20363.0
38	23247.0
39	26131.0
40	29697.5
41	33264.0
42	39313.0
43	45362.0
44	52141.5
45	58921.0
46	66365.5
47	73810.0
48	78607.0
49	83404.0
50	79725.5
51	76047.0
52	63219.5
53	50392.0
54	43871.5
55	37351.0
56	31449.5
57	25548.0
58	22742.5
59	19937.0
60	18866.5
61	17796.0
62	14902.5
63	12009.0
64	11241.0
65	10473.0
66	8009.5
67	5546.0
68	4668.5
69	3791.0
70	3266.5
71	2742.0
72	2221.5
73	1701.0
74	1154.0
75	593.5
76	580.0
77	495.0
78	410.0
79	258.0
80	106.0
81	79.0
82	52.0
83	35.0
84	18.0
85	12.0
86	6.0
87	3.5
88	1.0
89	0.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	681963.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.70438087222254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.31184888716099	18.50930011640681
2	12.6249575432302	6.742833494601165
3	5.353400615143653	4.288777469651596
4	2.8297649743033917	3.022684866106912
5	1.673100720991922	2.233955944547921
6	1.0782402247009513	1.7276242579299008
7	0.7591415520329469	1.419068359899257
8	0.5219016445699078	1.1149648235547305
9	0.41901276624477374	1.007052885011159
>10	3.7788269760893454	23.61222980349505
>50	1.4581601854449084	27.632340799443384
>100	0.1860890141424906	6.662396980439822
>500	0.002777447972275979	0.46712283952096273
>1k	0.002777447972275979	1.5596473593913631
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGC	2813	0.4124857213661151	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2613	0.3831586171097259	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGC	2280	0.3343289885228377	No Hit
CTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGCT	1696	0.24869384409418105	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGC	1112	0.1630586996655244	No Hit
ACTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGC	854	0.12522673517478222	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2896051545318441	0.0
2	0.0	0.0	0.0	1.4994948406291837	0.0
3	0.0	0.0	0.0	2.1369194516418046	0.0
4	0.0	0.0	0.0	2.831092009390539	0.0
5	0.0	0.0	0.0	4.688084250905108	0.0
6	0.0	0.0	0.0	5.7051482265166875	0.0
7	0.0	0.0	0.0	6.768695662374645	0.0
8	0.0	0.0	0.0	8.532134441311332	0.0
9	0.0	0.0	0.0	9.18671540831394	0.0
10	0.0	0.0	0.0	10.613185759344715	0.0
11	0.0	0.0	0.0	12.66051090748325	0.0
12	0.0	0.0	0.0	14.25678519215852	0.0
13	0.0	0.0	0.0	14.867228867255262	0.0
14	0.0	0.0	0.0	15.061081026389994	0.0
15	0.0	0.0	0.0	15.481338430384053	0.0
16	0.0	0.0	0.0	16.45059922605772	0.0
17	0.0	0.0	0.0	17.712837793252714	0.0
18	0.0	0.0	0.0	19.255736748181352	0.0
19	0.0	0.0	0.0	20.01853472989004	0.0
20	0.0	0.0	0.0	20.758164299236174	0.0
21	0.0	0.0	0.0	21.661145839290402	0.0
22	0.0	0.0	0.0	22.62674074693202	0.0
23	0.0	0.0	0.0	23.5820711680839	0.0
24	0.0	0.0	0.0	24.287534660971343	0.0
25	0.0	0.0	0.0	24.899298055759623	0.0
26	0.0	0.0	0.0	25.472789579493316	0.0
27	0.0	0.0	0.0	25.997891381203967	0.0
28	0.0	0.0	0.0	26.577101690267654	0.0
29	0.0	0.0	0.0	27.17640106574697	0.0
30	0.0	0.0	0.0	27.913537831231313	0.0
31	0.0	0.0	0.0	28.58322225692596	0.0
32	0.0	0.0	0.0	29.159793126606576	0.0
33	1.4663552128194637E-4	0.0	0.0	29.757919417915634	0.0
34	1.4663552128194637E-4	0.0	0.0	30.328038324659843	0.0
35	1.4663552128194637E-4	0.0	0.0	30.97953994571553	0.0
36	1.4663552128194637E-4	0.0	0.0	31.58763745247176	0.0
37	1.4663552128194637E-4	0.0	0.0	32.16083570516289	0.0
38	1.4663552128194637E-4	0.0	0.0	32.74297872465222	0.0
39	1.4663552128194637E-4	0.0	0.0	33.35459548391921	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	45	3.8380676E-10	45.000004	1
GGCACGA	30	2.1641972E-6	45.000004	8
ACGATTG	30	2.1641972E-6	45.000004	1
ACGATAG	30	2.1641972E-6	45.000004	1
CGGTCTA	45	3.8380676E-10	45.000004	31
GAACCGA	30	2.1641972E-6	45.000004	9
GTACGCG	30	2.1641972E-6	45.000004	1
TCACGAC	55	1.8189894E-12	45.0	25
CCTTCGT	20	7.0312666E-4	45.0	13
CTTCGCA	25	3.8891318E-5	45.0	16
ACTATTC	25	3.8891318E-5	45.0	10
CAAACGT	25	3.8891318E-5	45.0	14
GCCCACG	20	7.0312666E-4	45.0	10
CGACGAG	20	7.0312666E-4	45.0	2
TCCGATC	40	6.8084773E-9	45.0	13
CGGCCTA	20	7.0312666E-4	45.0	21
GCGCGGC	20	7.0312666E-4	45.0	25
GGCCGCG	20	7.0312666E-4	45.0	8
GAATCGG	20	7.0312666E-4	45.0	2
TAAACCG	20	7.0312666E-4	45.0	1
>>END_MODULE