SRR2934769.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934769.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1636376
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	8984	0.5490180740856625	Illumina PCR Primer Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	6142	0.37534160853006887	Illumina PCR Primer Index 10 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC	5097	0.31148097992148505	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	4733	0.28923670354490655	No Hit
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	4708	0.28770893731025143	Illumina PCR Primer Index 10 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG	4581	0.27994788483820343	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4546	0.2778090121096863	No Hit
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	3502	0.21400949415048864	Illumina PCR Primer Index 10 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	2713	0.1657931917847732	Illumina PCR Primer Index 10 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	2426	0.14825443541093244	Illumina PCR Primer Index 10 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	1688	0.10315477616391341	Illumina PCR Primer Index 10 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTG	30	2.1660671E-6	45.000004	1
TCGATAG	60	0.0	45.000004	1
ATCGCTC	30	2.1660671E-6	45.000004	20
TCGCACG	25	3.8915336E-5	45.0	1
GATCGGT	20	7.0341636E-4	45.0	9
CCGGCCG	25	3.8915336E-5	45.0	20
CGTAGAT	20	7.0341636E-4	45.0	33
CGACTCG	20	7.0341636E-4	45.0	44
CCCGCGT	25	3.8915336E-5	45.0	11
TGTCCGA	20	7.0341636E-4	45.0	20
ACGCGCC	25	3.8915336E-5	45.0	32
CGTTTTT	2880	0.0	42.343754	1
AGTTGCG	115	0.0	41.08696	1
TTGTCGC	50	1.0822987E-9	40.5	38
GTTGTCG	50	1.0822987E-9	40.5	37
GCGATAC	100	0.0	40.5	9
CGAGTGC	45	1.9292202E-8	40.000004	11
CGATTCG	45	1.9292202E-8	40.000004	10
TACGCGG	90	0.0	40.000004	2
ACGTCCG	45	1.9292202E-8	40.000004	1