##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934769.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1636376
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06715327039751	33.0	31.0	34.0	30.0	34.0
2	32.24169261832244	34.0	31.0	34.0	30.0	34.0
3	32.25302131050565	34.0	31.0	34.0	30.0	34.0
4	35.839008883043995	37.0	35.0	37.0	35.0	37.0
5	35.71623331068165	37.0	35.0	37.0	33.0	37.0
6	35.639574278772116	37.0	35.0	37.0	33.0	37.0
7	36.020508122827515	37.0	35.0	37.0	35.0	37.0
8	35.84853542217681	37.0	35.0	37.0	35.0	37.0
9	37.56601844563841	39.0	38.0	39.0	35.0	39.0
10	37.189267014427	39.0	37.0	39.0	34.0	39.0
11	37.104061658200806	39.0	37.0	39.0	34.0	39.0
12	37.074473715087485	39.0	37.0	39.0	33.0	39.0
13	37.04421416593741	39.0	37.0	39.0	33.0	39.0
14	38.1133914210426	40.0	37.0	41.0	33.0	41.0
15	38.16382115112908	40.0	37.0	41.0	33.0	41.0
16	38.27187516805429	40.0	37.0	41.0	34.0	41.0
17	38.26614482246134	40.0	37.0	41.0	34.0	41.0
18	38.25675456007666	40.0	37.0	41.0	34.0	41.0
19	38.22689773010604	40.0	37.0	41.0	34.0	41.0
20	38.15734953335908	40.0	37.0	41.0	34.0	41.0
21	38.11687656137709	40.0	37.0	41.0	34.0	41.0
22	38.08426119669318	40.0	37.0	41.0	34.0	41.0
23	37.8876150713528	40.0	36.0	41.0	33.0	41.0
24	37.61602773445712	39.0	36.0	41.0	33.0	41.0
25	37.568622370408754	39.0	36.0	41.0	33.0	41.0
26	37.771506670838484	39.0	36.0	41.0	33.0	41.0
27	37.849755190738556	40.0	37.0	41.0	33.0	41.0
28	37.80686101482789	40.0	36.0	41.0	33.0	41.0
29	37.85456704327123	40.0	37.0	41.0	33.0	41.0
30	37.729867096559715	40.0	36.0	41.0	33.0	41.0
31	37.61382408444025	40.0	36.0	41.0	33.0	41.0
32	37.5502586202682	40.0	36.0	41.0	33.0	41.0
33	37.487903146954004	40.0	36.0	41.0	33.0	41.0
34	37.39549773401712	40.0	36.0	41.0	32.0	41.0
35	37.384750815216066	40.0	36.0	41.0	32.0	41.0
36	37.32940840002542	40.0	36.0	41.0	32.0	41.0
37	37.24433137616293	40.0	36.0	41.0	32.0	41.0
38	37.17569250587884	40.0	36.0	41.0	32.0	41.0
39	37.19470525111588	40.0	36.0	41.0	32.0	41.0
40	37.09564611067383	39.0	36.0	41.0	31.0	41.0
41	37.031952314138074	39.0	36.0	41.0	31.0	41.0
42	36.95398429211868	39.0	35.0	41.0	31.0	41.0
43	36.92472940204452	39.0	35.0	41.0	31.0	41.0
44	36.89372919182389	39.0	35.0	41.0	31.0	41.0
45	36.84442267547312	39.0	35.0	41.0	31.0	41.0
46	36.754566798828634	39.0	35.0	41.0	31.0	41.0
47	36.603553828704406	39.0	35.0	41.0	31.0	41.0
48	36.58179232645798	39.0	35.0	41.0	31.0	41.0
49	36.582507932162294	39.0	35.0	41.0	31.0	41.0
50	36.470703554684256	39.0	35.0	41.0	31.0	41.0
51	35.44576368756325	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	10.0
10	19.0
11	16.0
12	13.0
13	9.0
14	25.0
15	34.0
16	64.0
17	128.0
18	292.0
19	510.0
20	952.0
21	1656.0
22	2707.0
23	3985.0
24	5838.0
25	8534.0
26	11382.0
27	13208.0
28	15423.0
29	18378.0
30	23062.0
31	30208.0
32	40001.0
33	55284.0
34	91685.0
35	117208.0
36	126691.0
37	197674.0
38	371405.0
39	499692.0
40	280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64800754838741	22.11863288144045	25.336108571624127	10.89725099854801
2	31.965391817039606	25.894476575065877	25.042716343920958	17.097415263973563
3	30.53754149413093	25.50911282003647	27.32587131563895	16.62747437019365
4	27.82306755904511	25.7995717365691	28.419446386405077	17.95791431798071
5	25.227514947664844	29.679303534151074	26.080252949199938	19.012928568984144
6	24.803590372872737	34.501422655917715	25.85322688673019	14.841760084479361
7	79.8062914635756	5.129566798828631	10.08252382093113	4.9816179166646295
8	80.19629962795837	4.804397033444636	9.04755386292637	5.951749475670629
9	74.92874498281569	6.4198570499689565	11.022222276542799	7.62917569067256
10	36.339753210753514	30.26706575994759	19.305220804998363	14.087960224300527
11	24.98215569037923	25.368313883850657	29.09789681589072	20.551633609879392
12	23.013965005597736	23.004126191046556	32.991684062831524	20.990224740524184
13	22.42522500941104	24.637430517191646	34.68609903836282	18.25124543503449
14	19.756950725261188	28.593733958454536	30.995443589981765	20.653871726302512
15	17.720743887712846	27.434464939598236	35.458965421150154	19.385825751538764
16	20.445362190596782	27.7827956410996	30.095650388419287	21.67619177988433
17	21.197695395190348	27.47088688663241	28.6851554899363	22.646262228240943
18	21.474709968858015	26.83001950651928	31.595427945655523	20.09984257896718
19	20.6716549252739	28.867326335756577	29.065752614313578	21.395266124655947
20	20.926119669318055	29.661153671283376	30.092655966599363	19.32007069279921
21	20.740526627132148	28.810737874424948	31.492823165336087	18.955912333106816
22	19.624462837391896	25.55983465902702	30.034417517734312	24.781284985846774
23	18.2418955056784	28.07050457840985	30.4375644717351	23.250035444176646
24	20.165903190953667	26.283506969058458	30.32438754907185	23.226202290916024
25	18.58778178120432	29.601265234884895	28.112854258434496	23.698098725476296
26	18.21323461111627	27.95543322561563	29.302434159386355	24.52889800388175
27	20.173480911477558	27.58687489916743	29.13676318890035	23.102881000454666
28	16.42147037111275	27.97492752276983	30.291143355805755	25.312458750311663
29	18.51579343622737	25.08763267121982	30.090883757767163	26.305690134785646
30	19.84012231907581	26.771536615056686	29.091113533808855	24.297227532058645
31	19.12683881944003	30.134027876233827	26.320662243885266	24.418471060440876
32	19.953910348232924	28.24546436760256	27.737451539255037	24.063173744909484
33	19.38625352608447	26.0507365055464	28.423846353160886	26.13916361520824
34	17.755760289811143	27.732868240551074	29.31117298224858	25.20019848738921
35	19.05197827394193	24.82351244457264	28.978181053743153	27.146328227742277
36	19.380875788938486	27.00363486142549	27.388204178012877	26.227285171623148
37	18.222706761771132	28.794421331038834	29.62577060528876	23.357101301901277
38	19.08791133578102	27.929400088977108	29.27896767002205	23.70372090521983
39	19.576796530870656	25.387135963861606	31.086437346917823	23.949630158349915
40	21.221772991048514	24.367627000151554	31.227786278948116	23.18281372985182
41	20.143658914577088	25.99873134291874	26.04108102294338	27.816528719560786
42	20.491256288285822	24.17323402445404	28.265569771250615	27.069939916009524
43	20.286413391543263	24.15875080054951	28.94114800021511	26.61368780769212
44	18.72625851271346	25.225070521689393	29.119163321877124	26.929507643720026
45	19.57850762905347	23.42878409363129	29.361222604095882	27.63148567321936
46	21.90902335404577	25.013016568319262	28.30266393542804	24.775296142206926
47	17.96048096525493	24.906745149036652	31.503884192874988	25.628889692833432
48	18.993923157025037	24.57442543767447	29.588493109163178	26.84315829613732
49	20.530122661295447	21.781729871374306	32.624714613267365	25.063432854062878
50	19.291959794081556	22.2637095630833	31.485001002214652	26.959329640620496
51	18.81254674964678	22.256192953208796	28.20030359770615	30.730956699438273
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	601.0
1	897.5
2	1194.0
3	4343.5
4	7493.0
5	5122.5
6	2752.0
7	2812.5
8	2873.0
9	2893.0
10	2913.0
11	3102.0
12	3291.0
13	3306.5
14	3322.0
15	3358.0
16	3394.0
17	3521.5
18	3649.0
19	3899.0
20	4149.0
21	4130.5
22	4112.0
23	4728.5
24	5345.0
25	6124.5
26	9165.5
27	11427.0
28	13879.0
29	16331.0
30	19657.0
31	22983.0
32	27624.0
33	32265.0
34	37069.0
35	41873.0
36	46099.5
37	50326.0
38	58094.0
39	65862.0
40	72744.5
41	79627.0
42	94016.0
43	108405.0
44	121506.0
45	134607.0
46	165440.0
47	196273.0
48	210887.0
49	225501.0
50	214488.5
51	203476.0
52	168512.5
53	133549.0
54	110243.5
55	86938.0
56	72191.5
57	57445.0
58	49291.0
59	41137.0
60	34652.5
61	28168.0
62	23304.5
63	18441.0
64	15356.5
65	12272.0
66	9818.0
67	7364.0
68	5818.5
69	4273.0
70	3265.0
71	2257.0
72	1991.5
73	1726.0
74	1298.5
75	687.5
76	504.0
77	372.0
78	240.0
79	175.0
80	110.0
81	75.5
82	41.0
83	42.5
84	44.0
85	40.5
86	37.0
87	21.5
88	6.0
89	4.0
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1636376.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.79454118203367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.47495948181286	19.429104995551135
2	13.220143587647065	7.613359379338035
3	5.763823194951194	4.978999330589491
4	2.9518774869347557	3.399918314474435
5	1.822189684882549	2.623455796141376
6	1.2103431052600393	2.091076463328043
7	0.8705434914193279	1.7546830290097644
8	0.6529091707708695	1.5040176004711403
9	0.510173663782793	1.322119491460437
>10	4.585857309677113	29.8803001675643
>50	0.8420016568223085	15.937317287180653
>100	0.08419607459116413	4.499872797873785
>500	0.007536729425067313	1.4941287417068763
>1k	0.0027993566435964303	2.2177243102722266
>5k	6.460053792914839E-4	1.2539222950383102
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	8984	0.5490180740856625	Illumina PCR Primer Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	6142	0.37534160853006887	Illumina PCR Primer Index 10 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC	5097	0.31148097992148505	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	4733	0.28923670354490655	No Hit
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	4708	0.28770893731025143	Illumina PCR Primer Index 10 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG	4581	0.27994788483820343	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4546	0.2778090121096863	No Hit
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	3502	0.21400949415048864	Illumina PCR Primer Index 10 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	2713	0.1657931917847732	Illumina PCR Primer Index 10 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	2426	0.14825443541093244	Illumina PCR Primer Index 10 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	1688	0.10315477616391341	Illumina PCR Primer Index 10 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.111064938620464E-5	0.0	0.0	0.3073254557632231	0.0
2	6.111064938620464E-5	0.0	0.0	1.6898316768273307	0.0
3	6.111064938620464E-5	0.0	0.0	2.4886089749544116	0.0
4	6.111064938620464E-5	0.0	0.0	3.3689078793626894	0.0
5	6.111064938620464E-5	0.0	0.0	5.901333189927009	0.0
6	6.111064938620464E-5	0.0	0.0	7.193823424445237	0.0
7	6.111064938620464E-5	0.0	0.0	8.595701721364772	0.0
8	6.111064938620464E-5	0.0	0.0	10.71226906285597	0.0
9	6.111064938620464E-5	0.0	0.0	11.438263577564081	0.0
10	6.111064938620464E-5	0.0	0.0	13.302871711635957	0.0
11	6.111064938620464E-5	0.0	0.0	15.739475523962708	0.0
12	6.111064938620464E-5	0.0	0.0	17.950886593301295	0.0
13	6.111064938620464E-5	0.0	0.0	18.68195329190846	0.0
14	1.2222129877240929E-4	0.0	0.0	18.950290153363287	0.0
15	1.2222129877240929E-4	0.0	0.0	19.467652911066896	0.0
16	1.2222129877240929E-4	0.0	0.0	20.5972221543215	0.0
17	1.2222129877240929E-4	0.0	0.0	22.111177382215335	0.0
18	1.2222129877240929E-4	0.0	0.0	23.67096559714882	0.0
19	1.2222129877240929E-4	0.0	0.0	24.71241328398852	0.0
20	1.8333194815861392E-4	0.0	0.0	25.613795362435038	0.0
21	2.4444259754481857E-4	0.0	0.0	26.724175861782378	0.0
22	2.4444259754481857E-4	0.0	0.0	27.977433059394663	0.0
23	2.4444259754481857E-4	0.0	0.0	29.175446229961818	0.0
24	2.4444259754481857E-4	0.0	0.0	30.113433587390674	0.0
25	2.4444259754481857E-4	0.0	0.0	30.92871076085203	0.0
26	2.4444259754481857E-4	0.0	0.0	31.669921827257305	0.0
27	2.4444259754481857E-4	0.0	0.0	32.452138139400724	0.0
28	2.4444259754481857E-4	0.0	0.0	33.210521298283524	0.0
29	0.0032388644174688457	0.0	0.0	34.00654861718822	0.0
30	0.0032388644174688457	0.0	0.0	34.884219763672895	0.0
31	0.003361085716241255	0.0	0.0	35.688802573491664	0.0
32	0.003361085716241255	0.0	0.0	36.44877460925851	0.0
33	0.003361085716241255	0.0	0.0	37.20868553437596	0.0
34	0.00342219636562746	0.0	0.0	37.9239245747921	0.0
35	0.00342219636562746	0.0	0.0	38.693368761213804	0.0
36	0.00342219636562746	0.0	0.0	39.39901342967631	0.0
37	0.00342219636562746	0.0	0.0	40.11584134697649	0.0
38	0.0034833070150136645	0.0	0.0	40.855524647147114	0.0
39	0.003544417664399869	0.0	0.0	41.68589615100686	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTG	30	2.1660671E-6	45.000004	1
TCGATAG	60	0.0	45.000004	1
ATCGCTC	30	2.1660671E-6	45.000004	20
TCGCACG	25	3.8915336E-5	45.0	1
GATCGGT	20	7.0341636E-4	45.0	9
CCGGCCG	25	3.8915336E-5	45.0	20
CGTAGAT	20	7.0341636E-4	45.0	33
CGACTCG	20	7.0341636E-4	45.0	44
CCCGCGT	25	3.8915336E-5	45.0	11
TGTCCGA	20	7.0341636E-4	45.0	20
ACGCGCC	25	3.8915336E-5	45.0	32
CGTTTTT	2880	0.0	42.343754	1
AGTTGCG	115	0.0	41.08696	1
TTGTCGC	50	1.0822987E-9	40.5	38
GTTGTCG	50	1.0822987E-9	40.5	37
GCGATAC	100	0.0	40.5	9
CGAGTGC	45	1.9292202E-8	40.000004	11
CGATTCG	45	1.9292202E-8	40.000004	10
TACGCGG	90	0.0	40.000004	2
ACGTCCG	45	1.9292202E-8	40.000004	1
>>END_MODULE